##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513164_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2241470 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.996405930036985 31.0 31.0 34.0 28.0 34.0 2 31.089430150749283 33.0 31.0 34.0 28.0 34.0 3 31.190924259526113 33.0 31.0 34.0 28.0 34.0 4 34.59279713759274 37.0 35.0 37.0 32.0 37.0 5 34.37118141219825 37.0 35.0 37.0 30.0 37.0 6 34.299665844289684 37.0 35.0 37.0 30.0 37.0 7 34.17793635426751 37.0 35.0 37.0 30.0 37.0 8 34.14785475603064 37.0 35.0 37.0 30.0 37.0 9 35.73573636943613 39.0 37.0 39.0 30.0 39.0 10 35.577507617768696 39.0 37.0 39.0 30.0 39.0 11 35.60567886253218 39.0 37.0 39.0 30.0 39.0 12 35.54298072247231 39.0 37.0 39.0 30.0 39.0 13 35.52378439149308 39.0 37.0 39.0 30.0 39.0 14 36.71818226431761 40.0 37.0 41.0 30.0 41.0 15 36.707485712501175 40.0 37.0 41.0 30.0 41.0 16 36.67534698211442 40.0 37.0 41.0 30.0 41.0 17 36.61271977764592 40.0 37.0 41.0 30.0 41.0 18 36.5660762802982 40.0 37.0 41.0 30.0 41.0 19 36.64423659473471 40.0 37.0 41.0 30.0 41.0 20 36.61508251281525 40.0 37.0 41.0 30.0 41.0 21 36.55952834523772 40.0 37.0 41.0 30.0 41.0 22 36.50944067955404 40.0 37.0 41.0 30.0 41.0 23 36.43875893944599 40.0 37.0 41.0 30.0 41.0 24 36.38772680428469 40.0 37.0 41.0 30.0 41.0 25 36.333946026491546 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5115061821963813 1101 2 0.5075854271592917 1101 3 0.5494066688273733 1101 4 0.5726650378693137 1101 5 0.5699647574240245 1101 6 0.5575086957691084 1101 7 0.6344171429659085 1101 8 0.6380145052882682 1101 9 0.7503042851790056 1101 10 0.7328679403338256 1101 11 0.76561131772349 1101 12 0.7033672682479732 1101 13 0.8339179506660912 1101 14 0.8814143517588349 1101 15 0.9223816505180196 1101 16 0.8624012151350655 1101 17 0.8036181211504498 1101 18 0.8752625690460007 1101 19 0.985984783474656 1101 20 0.9240553145342432 1101 21 0.9392010774683115 1101 22 0.8413353232842269 1101 23 0.887445808924447 1101 24 0.9807600849402291 1101 25 0.9312728799634229 1102 1 0.3534374820180908 1102 2 0.41996283833708503 1102 3 0.36443166613353384 1102 4 0.4647429322570318 1102 5 0.3506762521345479 1102 6 0.29343758527276265 1102 7 0.38856021146739295 1102 8 0.3828519807666808 1102 9 0.34598174198013254 1102 10 0.4175864550765809 1102 11 0.4985221512395981 1102 12 0.42695197059967427 1102 13 0.40551935635379976 1102 14 0.33184664619974313 1102 15 0.5028024206734827 1102 16 0.41420136889409775 1102 17 0.5309330275996942 1102 18 0.4882214866496568 1102 19 0.5590866197946482 1102 20 0.48296968939907003 1102 21 0.3986210536096806 1102 22 0.3972145429647682 1102 23 0.385125858689797 1102 24 0.38844763542584815 1102 25 0.40244019531693453 1103 1 0.3836781445299273 1103 2 0.3631704009919794 1103 3 0.36091992816387375 1103 4 0.46969189433099245 1103 5 0.4377333088459423 1103 6 0.3598364405094472 1103 7 0.36361363946399194 1103 8 0.41804235400417866 1103 9 0.46210108342491907 1103 10 0.5075608203065016 1103 11 0.5325781299861205 1103 12 0.6064379407264155 1103 13 0.6332958879384307 1103 14 0.7337137086785646 1103 15 0.648366327126837 1103 16 0.5118215267903565 1103 17 0.5883885831895626 1103 18 0.4944558836954016 1103 19 0.5325971702155456 1103 20 0.5881492642715926 1103 21 0.5157055942608579 1103 22 0.4714195894948787 1103 23 0.4999039906110241 1103 24 0.5514339771177816 1103 25 0.5062531065080336 1104 1 0.4078565345054592 1104 2 0.4599242046754277 1104 3 0.43820506421554484 1104 4 0.5665663370561518 1104 5 0.52132213480931 1104 6 0.48629726208070423 1104 7 0.5347654570983735 1104 8 0.6201732991459963 1104 9 0.6321416742627051 1104 10 0.7471992465457404 1104 11 0.6929157236221428 1104 12 0.5820799305709556 1104 13 0.6147080606854161 1104 14 0.7053174750534481 1104 15 0.6886474908588482 1104 16 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-0.9233861288819085 2216 24 -0.9157424392319271 2216 25 -1.0745858886317237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44258.0 3 1.0 4 773.0 5 6149.0 6 6878.0 7 5400.0 8 4442.0 9 3846.0 10 3470.0 11 3299.0 12 3060.0 13 2826.0 14 2766.0 15 2818.0 16 2879.0 17 3123.0 18 3445.0 19 4137.0 20 5212.0 21 7120.0 22 9028.0 23 10898.0 24 12517.0 25 14189.0 26 16162.0 27 17794.0 28 20137.0 29 24259.0 30 30916.0 31 39468.0 32 50947.0 33 66968.0 34 89354.0 35 120473.0 36 173840.0 37 285097.0 38 1143521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22701177295068 19.16017443328624 13.044372148776914 27.56844164498616 2 13.789912490680411 20.51616800825612 40.02133633642343 25.672583164640038 3 17.114047846377613 24.803644248529896 30.004327768210086 28.077980136882402 4 11.291785508899057 17.18622639160852 37.86078181793036 33.661206281562066 5 12.680859005768289 38.21621952270412 36.1765786666631 12.926342804864491 6 31.87393043693903 37.54185927940937 17.920986511475295 12.663223772176297 7 27.665587732189213 31.520208712012852 22.585863652611955 18.228339903185972 8 25.039042769457577 37.023655902463574 20.507984357904842 17.429316970174003 9 26.38397185276598 14.703726358630062 19.774716834489105 39.13758495411485 10 14.390770690034401 28.95821816995861 35.045625888682025 21.605385251324964 11 34.3315324318416 22.13355521153529 24.73582068910135 18.79909166752176 12 23.115990845293492 24.63798310929881 31.925343636095953 20.320682409311747 13 29.002261908479703 21.613003966147215 25.72811592392493 23.656618201448158 14 20.87991069994539 21.708398916407013 27.595795901905568 29.815894481742028 15 23.182197397243772 30.552316113978772 23.130311804306995 23.135174684470456 16 22.3067005135023 27.879962703047557 27.467733228640135 22.345603554810012 17 21.8860469952173 28.45514088837364 27.582269296571077 22.076542819837982 18 21.961494675294016 27.2993210849253 29.172685773397756 21.566498466382928 19 23.50493203121166 26.93509170321262 27.360526797146516 22.1994494684292 20 23.701633303144813 27.09891276706804 27.64770440826779 21.55174952151936 21 23.000368957429806 26.673079179513625 27.070678770135952 23.255873092920613 22 23.294689016305387 27.753731036981122 26.75322601081077 22.19835393590272 23 22.288824690752122 27.5317055759428 27.381437319442743 22.798032413862334 24 23.11527702802179 26.94463900922163 27.82482031880864 22.115263643947944 25 22.806715423425818 27.096896809098688 27.37524130402098 22.721146463454513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 182.0 2 307.0 3 432.0 4 432.0 5 432.0 6 941.0 7 1450.0 8 1450.0 9 1450.0 10 2234.0 11 3018.0 12 3018.0 13 3018.0 14 5566.5 15 8115.0 16 8115.0 17 8115.0 18 15735.5 19 23356.0 20 23356.0 21 23356.0 22 38297.5 23 53239.0 24 53239.0 25 53239.0 26 76822.0 27 100405.0 28 100405.0 29 100405.0 30 130643.0 31 160881.0 32 160881.0 33 160881.0 34 185379.5 35 209878.0 36 209878.0 37 209878.0 38 230020.5 39 250163.0 40 250163.0 41 250163.0 42 264092.0 43 278021.0 44 278021.0 45 278021.0 46 290720.5 47 303420.0 48 303420.0 49 303420.0 50 292577.0 51 281734.0 52 281734.0 53 281734.0 54 249896.5 55 218059.0 56 218059.0 57 218059.0 58 186600.5 59 155142.0 60 155142.0 61 155142.0 62 127080.5 63 99019.0 64 99019.0 65 99019.0 66 76519.0 67 54019.0 68 54019.0 69 54019.0 70 39541.0 71 25063.0 72 25063.0 73 25063.0 74 17635.0 75 10207.0 76 10207.0 77 10207.0 78 7003.0 79 3799.0 80 3799.0 81 3799.0 82 2576.5 83 1354.0 84 1354.0 85 1354.0 86 879.5 87 405.0 88 405.0 89 405.0 90 248.0 91 91.0 92 91.0 93 91.0 94 54.0 95 17.0 96 17.0 97 17.0 98 9.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18081883763780018 2 0.009948828224334924 3 0.0057997653325719285 4 0.0023645197125100937 5 0.0807951924406751 6 0.334468005371475 7 0.030292620467817995 8 0.015079389864687013 9 0.005041334481389445 10 0.003970608573837704 11 0.0 12 0.0 13 0.0 14 0.0016953160202902561 15 0.0 16 0.0 17 0.021236063833109522 18 0.030738756262631218 19 0.0 20 0.0 21 8.476580101451281E-4 22 8.922715896264505E-5 23 0.0064689690247917665 24 0.0 25 1.3384073844396757E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2241470.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.88057524773853 #Duplication Level Percentage of deduplicated Percentage of total 1 81.61342960744368 57.03193408908981 2 12.377504734073783 17.29894301897366 3 3.219218792387451 6.748825831810958 4 1.1620326649827621 3.248140443426322 5 0.5214362792206185 1.821913357348862 6 0.30242574729648114 1.2680211114483186 7 0.17594078543279631 0.860639030690792 8 0.11597266131401526 0.6483389028507631 9 0.0836678854292165 0.5262083967199976 >10 0.3896972259601448 4.734228747669127 >50 0.021809999453449427 1.0648641795479021 >100 0.013417314204285186 1.858790188961385 >500 0.0022337147786802266 1.0971573216121977 >1k 0.0012125880227117137 1.7919953798499324 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3556 0.1586458886355829 No Hit CCCATGTACTCTGCGTTGATACCAC 3497 0.15601368744618488 No Hit GTATCAACGCAGAGTACTTTTTTTT 3298 0.1471355851294017 No Hit GTCCTACAGTGGACATTTCTAAATT 3282 0.14642176785770053 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3224 0.14383418024778383 No Hit CTTTAGGACGTGAAATATGGCGAGG 3038 0.13553605446425784 No Hit CTGTAGGACGTGGAATATGGCAAGA 2878 0.12839788174724623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.461357948132253E-5 2 0.0 0.0 0.0 0.0 4.461357948132253E-5 3 0.0 0.0 0.0 0.0 4.461357948132253E-5 4 0.0 0.0 0.0 0.0 4.461357948132253E-5 5 0.0 0.0 0.0 0.0 4.461357948132253E-5 6 0.0 0.0 0.0 0.0 4.461357948132253E-5 7 0.0 0.0 0.0 0.0 4.461357948132253E-5 8 0.0 0.0 0.0 0.0 4.461357948132253E-5 9 0.0 0.0 0.0 0.0 4.461357948132253E-5 10 0.0 0.0 0.0 0.0 4.461357948132253E-5 11 0.0 0.0 0.0 0.0 4.461357948132253E-5 12 0.0 4.461357948132253E-5 0.0 0.0 8.922715896264505E-5 13 0.0 8.922715896264505E-5 0.0 0.0 1.3384073844396757E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATAC 50 0.001474804 13.32982 3 TCCAACG 70 1.1017895E-4 12.201793 18 TAGGACC 795 0.0 11.616664 4 CTAGTAC 140 8.069037E-9 10.881487 3 TACACGG 90 9.308969E-5 10.578749 5 GGCGAGG 945 0.0 10.542641 19 TGGCGAG 1915 0.0 10.456656 18 GTCCTAC 1780 0.0 10.274306 1 AAGTGCG 75 0.0026674701 10.12455 7 GCAACTA 75 0.0026704343 10.123195 13 GTATTAC 170 1.4897523E-9 10.085441 1 GTAGGAC 1930 0.0 10.014632 3 TGTAGGA 1955 0.0 9.984866 2 TTAGGAC 1285 0.0 9.928827 3 CTGTAGG 1930 0.0 9.919963 1 GGACGTG 1860 0.0 9.8275585 6 GTCCTAA 1135 0.0 9.818865 1 GTACTAG 165 9.79162E-9 9.813779 1 TCTAGTA 205 4.0017767E-11 9.7544 2 TAAGACT 245 0.0 9.715178 4 >>END_MODULE