FastQCFastQC Report
Thu 26 May 2016
SRR1513163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977595
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC20430.10330730002857005No Hit
GTATCAACGCAGAGTACTTTTTTTT20340.10285220179055875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATC400.005323390514.2323399
TAGGACC3900.013.4406664
GGACGTG7350.012.13429456
GGCGAGG4350.011.7793819
AGGACGT7800.011.4603285
GTAGGAC8000.011.4370763
ATAGGAC2400.011.1193793
GGACCGC600.005932223511.0693186
TAGGACG7750.011.0678564
TTAGGAC6550.011.0587493
GTCCTAC9900.010.903911
GTCTTAG2300.010.7990261
GACGTGG4100.010.6453277
TAGGACA4350.010.5165834
GTCTAGG1105.658356E-610.4214361
CGCAGAT1106.1387327E-610.350538
TCCTACA11350.010.2522172
TGGCGAG8450.010.21886418
CTAGGAC2550.010.09153653
GTATAGT1908.913048E-1110.0557721
Produced by FastQC (version 0.11.5)