##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513163_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1977595 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.54188243801183 31.0 30.0 34.0 25.0 34.0 2 29.371693395260404 31.0 30.0 34.0 22.0 34.0 3 29.553524356604868 31.0 30.0 34.0 23.0 34.0 4 32.6174965045927 37.0 33.0 37.0 22.0 37.0 5 32.76368164361257 37.0 33.0 37.0 25.0 37.0 6 33.12994672822292 37.0 35.0 37.0 27.0 37.0 7 33.08829714880954 37.0 35.0 37.0 26.0 37.0 8 32.96904876883285 37.0 35.0 37.0 26.0 37.0 9 34.22206063425524 39.0 35.0 39.0 25.0 39.0 10 33.79953529413252 39.0 34.0 39.0 19.0 39.0 11 34.2709801551885 39.0 35.0 39.0 26.0 39.0 12 34.266973268035166 39.0 35.0 39.0 26.0 39.0 13 34.31020760064624 39.0 35.0 39.0 26.0 39.0 14 34.534911344334915 40.0 34.0 41.0 17.0 41.0 15 34.979936741344915 40.0 34.0 41.0 22.0 41.0 16 34.8331468273332 40.0 34.0 41.0 19.0 41.0 17 34.44806241925167 39.0 34.0 41.0 17.0 41.0 18 34.18409583357563 39.0 33.0 41.0 17.0 41.0 19 34.82520637440932 40.0 34.0 41.0 19.0 41.0 20 35.037894513285075 40.0 35.0 41.0 24.0 41.0 21 35.07995974908917 40.0 36.0 41.0 24.0 41.0 22 34.769239910092814 40.0 35.0 41.0 18.0 41.0 23 34.316994632369116 40.0 34.0 41.0 11.0 41.0 24 34.566301492469385 40.0 34.0 41.0 18.0 41.0 25 34.20854168826276 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0236032528966348 1101 2 1.1640585984110956 1101 3 1.150355947702451 1101 4 1.5126968671745757 1101 5 1.4057185465346862 1101 6 1.0090583745376733 1101 7 1.033051621729065 1101 8 1.0311228720139098 1101 9 1.443600768817376 1101 10 1.710994809346147 1101 11 1.3585279712056604 1101 12 1.2918581746492777 1101 13 1.2463453303722076 1101 14 2.2707234669931893 1101 15 1.8148417677772386 1101 16 1.8491916482857107 1101 17 2.2526764075028183 1101 18 2.276543933104726 1101 19 1.8287209544217617 1101 20 1.4695413186837598 1101 21 1.5617930319485325 1101 22 1.6899578481430453 1101 23 2.009857042270667 1101 24 1.6356559873577652 1101 25 1.9367877123254473 1102 1 1.2038140969241695 1102 2 1.4396085555021223 1102 3 1.3125263770470923 1102 4 1.5381222041445497 1102 5 1.5605736803644206 1102 6 1.275525932238203 1102 7 1.2956085150627388 1102 8 1.3384219291494048 1102 9 1.8107304653201766 1102 10 1.9042124064625128 1102 11 1.5344245516106554 1102 12 1.4612140868827197 1102 13 1.4596732635002994 1102 14 2.52284035689037 1102 15 2.106811369908627 1102 16 2.310460989186886 1102 17 2.6661872112176326 1102 18 2.9626886302825497 1102 19 2.1826293529682346 1102 20 1.9564963298759679 1102 21 1.9845901588882882 1102 22 2.0953933624229037 1102 23 2.5481182517089422 1102 24 2.1463093555469683 1102 25 2.3439771131953293 1103 1 0.769170053640547 1103 2 1.0738378222674427 1103 3 0.7816580953829906 1103 4 1.2838803198234956 1103 5 1.089095506685723 1103 6 0.8528508613227004 1103 7 0.8222889610197157 1103 8 0.8340286155220724 1103 9 1.0677167146072932 1103 10 1.2493095448679696 1103 11 0.9428685292904433 1103 12 0.8590803780668352 1103 13 0.8162375608496788 1103 14 1.8269644525079585 1103 15 1.3437576977712453 1103 16 1.3997124904337355 1103 17 1.8604369978187947 1103 18 1.9943299003131543 1103 19 1.435976639541444 1103 20 0.9578894794937085 1103 21 1.1345219458100146 1103 22 1.3226966720618236 1103 23 1.749865419747941 1103 24 1.5366950390010103 1103 25 1.6215973527238816 1104 1 0.9210458133678436 1104 2 1.1423364152617808 1104 3 0.9400549639125302 1104 4 1.3380416738201717 1104 5 1.1527155817012442 1104 6 1.0373553983118882 1104 7 0.9173306135915453 1104 8 1.0118192077283368 1104 9 1.354275628555051 1104 10 1.4325673856254113 1104 11 1.0932448150185365 1104 12 1.103809682816454 1104 13 1.0490031354916098 1104 14 2.052605783159862 1104 15 1.4864553941479741 1104 16 1.7155411182593596 1104 17 2.0437036672114957 1104 18 2.1139843939955654 1104 19 1.5123991650143793 1104 20 1.3644295466285143 1104 21 1.3022799557055222 1104 22 1.5595503694942678 1104 23 1.8451463748018284 1104 24 1.6777264466386157 1104 25 1.9484775732353086 1105 1 0.5837886599455118 1105 2 0.8652916666423991 1105 3 0.7323556101609618 1105 4 1.0551777832316205 1105 5 0.9318468738823285 1105 6 0.5658222962141579 1105 7 0.5551977466346969 1105 8 0.6327798117905346 1105 9 0.8991840824144859 1105 10 1.1354182743600205 1105 11 0.6848471321951237 1105 12 0.6503765480882322 1105 13 0.632650684108981 1105 14 1.4499838112623564 1105 15 0.9886307219287787 1105 16 1.204020679354116 1105 17 1.5900407918750687 1105 18 1.137133162938298 1105 19 1.0572289029682125 1105 20 0.7422825977622765 1105 21 0.7686219268890895 1105 22 0.9965916033569329 1105 23 1.22953925320909 1105 24 1.0953428146043578 1105 25 1.2688989046965489 1106 1 0.30887721096470955 1106 2 0.4870295852247928 1106 3 0.4230769926421658 1106 4 0.846642599617752 1106 5 0.7744307876893828 1106 6 0.41149316268217717 1106 7 0.5290838290784095 1106 8 0.46673308447834216 1106 9 0.6293242347518415 1106 10 0.5596812536208304 1106 11 0.5820243526304338 1106 12 0.5787834190553411 1106 13 0.38590289911775955 1106 14 1.4418958226284602 1106 15 0.9926697830413218 1106 16 1.1911117167083418 1106 17 1.40731860034834 1106 18 1.4279603626541473 1106 19 0.9032253609274434 1106 20 0.796907970219209 1106 21 0.6251199437409198 1106 22 0.728655705415072 1106 23 1.136198092524758 1106 24 0.7632801925075441 1106 25 1.2336363595577282 1107 1 0.6199183885130815 1107 2 0.7846748342732042 1107 3 0.7720675378021191 1107 4 1.0618577562823006 1107 5 0.8757182428491745 1107 6 0.5805415398123728 1107 7 0.6004089527089533 1107 8 0.6537663825442621 1107 9 0.998786569873694 1107 10 1.1068178121560521 1107 11 0.7291438013810136 1107 12 0.7866585454701536 1107 13 0.6799245562430656 1107 14 1.5917615938980347 1107 15 1.0117980507407367 1107 16 1.1016447201805946 1107 17 1.5417560387126983 1107 18 1.6616202937838978 1107 19 1.1108250916616456 1107 20 0.896502634481223 1107 21 0.7418845687896436 1107 22 1.0335921477295003 1107 23 1.3791134740864308 1107 24 1.0529305466486534 1107 25 1.3438072059217419 1108 1 0.46431717287322627 1108 2 0.8447839039610869 1108 3 0.5287942795569336 1108 4 1.0291312108980364 1108 5 0.7964297632326804 1108 6 0.543052898013265 1108 7 0.4426364935459546 1108 8 0.5253744295794291 1108 9 0.7176957270437754 1108 10 1.0172635534029126 1108 11 0.6752472722968292 1108 12 0.43217741595028514 1108 13 0.41072375121225946 1108 14 1.5000060168527796 1108 15 0.81925606271448 1108 16 0.9955837254531872 1108 17 1.2955535864248802 1108 18 1.453813812538499 1108 19 0.8552305765034092 1108 20 0.679951803588331 1108 21 0.7157940432927603 1108 22 0.9795091993052907 1108 23 1.411723258602919 1108 24 0.9689352551190993 1108 25 1.2420626039557803 1109 1 0.5571467242083479 1109 2 0.7685454532245046 1109 3 0.5578198217645713 1109 4 0.9562555787870295 1109 5 1.0652701868663215 1109 6 0.585686852733609 1109 7 0.4584248427731197 1109 8 0.582359127206125 1109 9 0.7654108034469687 1109 10 1.1138675684420818 1109 11 0.7642613910275315 1109 12 0.6551510365426196 1109 13 0.5881436494050902 1109 14 1.6226911381435443 1109 15 1.1320345996183292 1109 16 1.3229638175234513 1109 17 1.6302931926583284 1109 18 1.878705212351285 1109 19 1.2801835926729765 1109 20 1.1016894674394848 1109 21 0.9119581371052377 1109 22 1.1944102077922665 1109 23 1.6054423675630218 1109 24 1.3373817252842883 1109 25 1.4333016273875998 1110 1 0.4377043610519742 1110 2 0.7162966320987856 1110 3 0.6364888438249778 1110 4 1.036646063062463 1110 5 0.9497817617340729 1110 6 0.6006528209323392 1110 7 0.576473405026988 1110 8 0.6319498795210308 1110 9 0.761865525302099 1110 10 1.1252488916825598 1110 11 0.6850354094483961 1110 12 0.7394879090423601 1110 13 0.5840762486749824 1110 14 1.3704512473524773 1110 15 1.0071270442208657 1110 16 1.1669297316871905 1110 17 1.3315874117011006 1110 18 1.0597193208779316 1110 19 0.8343876501726868 1110 20 0.8072087701744834 1110 21 0.6206122245830414 1110 22 0.9002138144179312 1110 23 -0.34597499410765664 1110 24 0.5524569714428154 1110 25 0.9490556329069335 1111 1 0.5253019351464232 1111 2 0.8630074143862281 1111 3 0.7676698361877854 1111 4 1.1554598107817284 1111 5 1.0011588605852566 1111 6 0.7014610060136235 1111 7 0.6686929030246063 1111 8 0.6934348134936243 1111 9 0.8680782891393193 1111 10 1.167467348164088 1111 11 0.7657534295922588 1111 12 0.7449287053426445 1111 13 0.5891706754543549 1111 14 1.4594243554071014 1111 15 1.182504536231285 1111 16 1.1422017322490845 1111 17 1.5839625655864111 1111 18 1.6927446341152006 1111 19 1.2890600849892166 1111 20 0.8951320928180593 1111 21 0.8336328295395532 1111 22 1.0823744547189875 1111 23 1.4163749006573525 1111 24 1.1344797129204096 1111 25 1.258623209035214 1112 1 0.06440569930474993 1112 2 0.25837688937606984 1112 3 0.133905525086071 1112 4 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24 -2.2857399587902236 2216 25 -1.9283044256255266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89135.0 3 5.0 4 754.0 5 4393.0 6 5339.0 7 4773.0 8 4391.0 9 4175.0 10 3851.0 11 3793.0 12 3656.0 13 3540.0 14 3557.0 15 3700.0 16 3738.0 17 3936.0 18 4620.0 19 5637.0 20 7284.0 21 9219.0 22 11263.0 23 13585.0 24 15885.0 25 17596.0 26 20179.0 27 22827.0 28 25485.0 29 30504.0 30 36797.0 31 43777.0 32 56215.0 33 73876.0 34 97213.0 35 130383.0 36 186571.0 37 272404.0 38 753539.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77009459018685 18.320087195580253 12.278845549966269 25.630972664266626 2 14.609571788413097 20.237986590248116 40.97478268981938 24.177658931519407 3 18.476887539571965 24.819758704817993 29.616762896759003 27.08659085885104 4 11.56109804240358 16.779289005858054 38.98284245235567 32.6767704993827 5 12.930956249730663 37.81306956594481 36.0798906940371 13.176083490287436 6 31.896836868772183 36.96572659160498 18.254436283707715 12.883000255915128 7 28.09447584366979 31.354347633678515 22.42289066747588 18.128285855175815 8 25.351357611724108 35.861816787785514 20.970849038997926 17.815976561492448 9 26.559163604326418 14.943310038305805 19.929336056312668 38.568190301055104 10 13.954470508502007 28.531747235962328 35.55265580270891 21.961126452826754 11 34.55142074794735 22.341768502699274 24.384710079617722 18.722100669735656 12 23.150747429746055 24.670189100686784 31.479503217432914 20.69956025213425 13 28.846367839114645 21.629035031814915 25.650410205051944 23.874186924018492 14 20.75322920175569 21.310759015413375 28.59678298772858 29.339228795102358 15 23.547989410949942 29.52266816541622 23.377305984189274 23.552036439444564 16 22.60953434878221 27.61533045921784 27.244399622089553 22.530735569910398 17 21.911992650540483 27.773247319835043 27.89961674349334 22.415143286131133 18 21.815145025255873 27.171939224043307 29.161047787367107 21.851867963333717 19 23.51002756632356 26.93128651273362 27.276548392807463 22.282137528135355 20 23.87717785235748 26.851778321248553 27.379040518359844 21.892003308034123 21 23.62776802348315 26.522643705546088 26.720832087700636 23.128756183270127 22 23.34359451713837 27.257228569766355 26.925514935207833 22.473661977887442 23 22.774766226087078 26.974983017343806 27.336284918136766 22.913965838432347 24 23.242809521569033 26.86892235026993 27.323792741804514 22.56447538635652 25 23.11999866454005 26.699031336264262 27.329783235627236 22.85118676356845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 210.0 1 210.0 2 329.5 3 449.0 4 449.0 5 449.0 6 839.0 7 1229.0 8 1229.0 9 1229.0 10 1950.5 11 2672.0 12 2672.0 13 2672.0 14 5077.5 15 7483.0 16 7483.0 17 7483.0 18 14221.5 19 20960.0 20 20960.0 21 20960.0 22 33392.0 23 45824.0 24 45824.0 25 45824.0 26 66081.5 27 86339.0 28 86339.0 29 86339.0 30 110912.5 31 135486.0 32 135486.0 33 135486.0 34 158324.0 35 181162.0 36 181162.0 37 181162.0 38 199833.5 39 218505.0 40 218505.0 41 218505.0 42 232036.0 43 245567.0 44 245567.0 45 245567.0 46 254883.0 47 264199.0 48 264199.0 49 264199.0 50 256316.5 51 248434.0 52 248434.0 53 248434.0 54 223282.0 55 198130.0 56 198130.0 57 198130.0 58 170053.5 59 141977.0 60 141977.0 61 141977.0 62 116066.5 63 90156.0 64 90156.0 65 90156.0 66 70037.0 67 49918.0 68 49918.0 69 49918.0 70 36768.0 71 23618.0 72 23618.0 73 23618.0 74 16595.0 75 9572.0 76 9572.0 77 9572.0 78 6674.0 79 3776.0 80 3776.0 81 3776.0 82 2567.5 83 1359.0 84 1359.0 85 1359.0 86 887.0 87 415.0 88 415.0 89 415.0 90 264.5 91 114.0 92 114.0 93 114.0 94 73.5 95 33.0 96 33.0 97 33.0 98 20.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16095307684333748 2 0.26795172924688826 3 0.2649177409934794 4 0.47355499988622546 5 0.26041732508425636 6 0.01906355952558537 7 0.018709594229354344 8 0.01699033421908935 9 0.01764769834066126 10 0.02098508541941095 11 0.020074888943388308 12 0.02796325840225122 13 0.027053061926228576 14 0.024322472498160642 15 0.042273569664162784 16 0.020580553652289776 17 0.043032066727514987 18 0.03180631018990238 19 0.027558726635130044 20 0.029834217825186655 21 0.03478973197242105 22 0.04500415909223072 23 0.02922742017450489 24 0.049959673239465104 25 0.03782372022582986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1977595.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.4439092525815 #Duplication Level Percentage of deduplicated Percentage of total 1 83.94633612567792 63.3323976475235 2 11.20278990593682 16.90364530078467 3 2.716415190744272 6.148109434285342 4 0.924441760445921 2.7897400113751503 5 0.43451083404315227 1.639059796640754 6 0.21640154281877214 0.9795707015122846 7 0.12917441060099968 0.6821795757796266 8 0.08718493121068578 0.5262057630761231 9 0.05959129230810922 0.4046220044223346 >10 0.2580912990342218 3.375952062556004 >50 0.014763378748551402 0.7694011157706738 >100 0.008606495926943621 1.2547361768564504 >500 0.0010156995021237665 0.45021704654379113 >1k 6.77133001415775E-4 0.7441633628733476 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2043 0.10330730002857005 No Hit GTATCAACGCAGAGTACTTTTTTTT 2034 0.10285220179055875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.056647089014687E-5 7 0.0 0.0 0.0 0.0 5.056647089014687E-5 8 0.0 0.0 0.0 0.0 5.056647089014687E-5 9 0.0 0.0 0.0 0.0 5.056647089014687E-5 10 0.0 0.0 0.0 0.0 5.056647089014687E-5 11 0.0 0.0 0.0 0.0 5.056647089014687E-5 12 0.0 0.0 0.0 0.0 5.056647089014687E-5 13 0.0 0.0 0.0 0.0 5.056647089014687E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAATC 40 0.0053233905 14.232339 9 TAGGACC 390 0.0 13.440666 4 GGACGTG 735 0.0 12.1342945 6 GGCGAGG 435 0.0 11.77938 19 AGGACGT 780 0.0 11.460328 5 GTAGGAC 800 0.0 11.437076 3 ATAGGAC 240 0.0 11.119379 3 GGACCGC 60 0.0059322235 11.069318 6 TAGGACG 775 0.0 11.067856 4 TTAGGAC 655 0.0 11.058749 3 GTCCTAC 990 0.0 10.90391 1 GTCTTAG 230 0.0 10.799026 1 GACGTGG 410 0.0 10.645327 7 TAGGACA 435 0.0 10.516583 4 GTCTAGG 110 5.658356E-6 10.421436 1 CGCAGAT 110 6.1387327E-6 10.35053 8 TCCTACA 1135 0.0 10.252217 2 TGGCGAG 845 0.0 10.218864 18 CTAGGAC 255 0.0 10.0915365 3 GTATAGT 190 8.913048E-11 10.055772 1 >>END_MODULE