FastQCFastQC Report
Thu 26 May 2016
SRR1513163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977595
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30020.1518005456122209No Hit
GTACATGGGAAGCAGTGGTATCAAC21610.10927414359360739No Hit
CCCATGTACTCTGCGTTGATACCAC21590.1091730106518271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3950.011.8104614
TTACACG657.880051E-411.7177564
AGTACGT752.0328841E-411.4248135
TAGTACG855.2076903E-511.2007974
CTTACAC2150.011.0708363
CGCCTTT700.001503475710.84692512
GTCCTAC9150.010.720531
TGGCGAG9050.010.69656418
GGACGTG7650.010.5782616
AGGACGT8050.010.4076985
TTAGTAC1752.2737368E-1010.3369983
GTCTTAT1951.4551915E-1110.256161
TGTGCGG856.6705426E-410.04630810
TCCTACA10150.010.0368312
ATGGCGA10050.010.0104717
GGCGAGA5700.09.98755719
TGTAGGA10400.09.9786562
TATGGCG9650.09.93364716
GACGTGA4650.09.7984617
GTCTTAC2054.0017767E-119.7558591
Produced by FastQC (version 0.11.5)