##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513163_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1977595 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.74907096751357 31.0 31.0 34.0 27.0 34.0 2 30.856256210194704 33.0 31.0 34.0 27.0 34.0 3 30.960177387179883 33.0 31.0 34.0 27.0 34.0 4 34.34112191828964 37.0 35.0 37.0 31.0 37.0 5 34.079742818929056 37.0 35.0 37.0 30.0 37.0 6 33.9906325612676 37.0 35.0 37.0 30.0 37.0 7 33.85551035474908 37.0 35.0 37.0 29.0 37.0 8 33.824554572599546 37.0 35.0 37.0 29.0 37.0 9 35.3852472321178 39.0 35.0 39.0 29.0 39.0 10 35.228496734670145 39.0 35.0 39.0 28.0 39.0 11 35.240092132109964 39.0 35.0 39.0 29.0 39.0 12 35.17103148015645 39.0 35.0 39.0 28.0 39.0 13 35.13526733228998 39.0 35.0 39.0 28.0 39.0 14 36.294232135497914 40.0 37.0 41.0 27.0 41.0 15 36.27679580500558 40.0 37.0 41.0 27.0 41.0 16 36.24545622334199 40.0 37.0 41.0 27.0 41.0 17 36.17520270834018 40.0 36.0 41.0 27.0 41.0 18 36.129417297272695 40.0 36.0 41.0 27.0 41.0 19 36.21448678824532 40.0 37.0 41.0 27.0 41.0 20 36.176435013235775 40.0 37.0 41.0 27.0 41.0 21 36.1273385096544 40.0 37.0 41.0 27.0 41.0 22 36.06821416923081 40.0 37.0 41.0 27.0 41.0 23 35.98699582068118 40.0 37.0 41.0 27.0 41.0 24 35.92256857445533 40.0 37.0 41.0 27.0 41.0 25 35.86723874200734 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4406225790584948 1101 2 0.4499609961315869 1101 3 0.4045386691247863 1101 4 0.6369567107693186 1101 5 0.5624819724622654 1101 6 0.6061474202967716 1101 7 0.5655425938152376 1101 8 0.6731353252803771 1101 9 0.6451865178964553 1101 10 0.8179214649988324 1101 11 0.6813260204885054 1101 12 0.6077930311669419 1101 13 0.6762302884579441 1101 14 0.7630041893404282 1101 15 0.8565954685507364 1101 16 0.7252835755155189 1101 17 0.6788787697827203 1101 18 0.8256263458776942 1101 19 0.8441067912797919 1101 20 0.8761627118662219 1101 21 0.8655390339881919 1101 22 0.8105759361513805 1101 23 0.9133204941375439 1101 24 0.8184666948346759 1101 25 0.8273440522276587 1102 1 0.5856180899018604 1102 2 0.5915315778526455 1102 3 0.5128540883157164 1102 4 0.6221849780115036 1102 5 0.5896557674942642 1102 6 0.6651869107137642 1102 7 0.6419340199509094 1102 8 0.6606716610145327 1102 9 0.6570214170060851 1102 10 0.7684437365970567 1102 11 0.8893925298694754 1102 12 0.7719769723537979 1102 13 0.8932212978714844 1102 14 0.8942711888938746 1102 15 0.8715555476278638 1102 16 0.8384249560987627 1102 17 1.0007324185560194 1102 18 0.9273055075996766 1102 19 0.995120651491888 1102 20 0.9413874198822256 1102 21 0.9751223685016441 1102 22 0.9916508602227978 1102 23 0.9816635374553115 1102 24 1.0462392329028205 1102 25 1.098206829424818 1103 1 0.2804195451141993 1103 2 0.2501102111309379 1103 3 0.36074731061953 1103 4 0.40847582664929405 1103 5 0.2814263068994336 1103 6 0.24359234614058067 1103 7 0.24826115570984797 1103 8 0.3042024146758635 1103 9 0.31086194377505905 1103 10 0.33086988568923204 1103 11 0.3357837598295532 1103 12 0.2981462861600477 1103 13 0.34891820815451524 1103 14 0.43850904752505926 1103 15 0.41389797728079714 1103 16 0.4355037217029647 1103 17 0.4264919879562967 1103 18 0.46115578175660943 1103 19 0.3249998435210202 1103 20 0.44023795496350004 1103 21 0.3820977148787108 1103 22 0.3187712432597323 1103 23 0.35819227454421565 1103 24 0.4108511824922374 1103 25 0.48493817426615493 1104 1 0.3821021900618007 1104 2 0.5353894376309682 1104 3 0.44268912712517405 1104 4 0.6086674545009458 1104 5 0.59568994295892 1104 6 0.5552956779745983 1104 7 0.4861078422765672 1104 8 0.5448022414487426 1104 9 0.5423501542943328 1104 10 0.568985613298878 1104 11 0.5814354246313655 1104 12 0.6432441631985313 1104 13 0.6640094126033986 1104 14 0.7212112418591232 1104 15 0.6910911032124503 1104 16 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-1.3622489543790621 2215 22 -1.4313425655414846 2215 23 -1.2310530521625083 2215 24 -1.2974695743311173 2215 25 -1.3850395601427579 2216 1 -0.27691902931337964 2216 2 -0.24894588369351567 2216 3 -0.23093991255178636 2216 4 -0.1121882494417008 2216 5 -0.43508147851020595 2216 6 -0.5180919254945664 2216 7 -0.6935753057305831 2216 8 -0.5641506672361061 2216 9 -0.8041427083150623 2216 10 -0.7000674909228763 2216 11 -0.6630414896827617 2216 12 -0.6349246992621005 2216 13 -0.6618586127087411 2216 14 -0.7777517666297982 2216 15 -0.7659373621849568 2216 16 -0.7761124300411737 2216 17 -0.8041723226938586 2216 18 -0.7090729916162317 2216 19 -0.6808311018696926 2216 20 -0.7487914196626733 2216 21 -0.9650147640598306 2216 22 -0.8215085271850313 2216 23 -0.8456445270070034 2216 24 -0.8095980764962647 2216 25 -0.9463495750995534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48389.0 3 1.0 4 803.0 5 6953.0 6 7549.0 7 5818.0 8 4845.0 9 4297.0 10 3778.0 11 3454.0 12 3104.0 13 3035.0 14 2914.0 15 2899.0 16 2877.0 17 3136.0 18 3522.0 19 3994.0 20 5203.0 21 6638.0 22 8334.0 23 10153.0 24 12004.0 25 13301.0 26 14698.0 27 16285.0 28 18222.0 29 22007.0 30 27885.0 31 35618.0 32 45682.0 33 59111.0 34 78918.0 35 106626.0 36 152810.0 37 249686.0 38 983046.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.03265201091507 19.118446359979554 13.28555921000977 27.563342419095598 2 14.052213543115316 20.60712504892744 39.19990614022933 26.140755267727915 3 17.02457222005616 24.72332168718018 29.935114289572017 28.316991803191645 4 11.354913484206278 17.228571804215097 37.49063238600334 33.92588232557528 5 12.937052497663334 37.894304306396364 35.86951516289351 13.299128033046795 6 32.0441473288766 37.30913934723514 17.806238108064374 12.84047521582389 7 27.761277374034925 31.496679911949887 22.415348882378648 18.326693831636533 8 25.3464932931643 36.19344655443681 20.51437533094187 17.94568482145702 9 26.340291448946374 14.723142717139174 19.57401748370037 39.362548350214084 10 14.377705408794855 28.816901978235364 34.78462923257413 22.020763380395646 11 34.50044119245852 22.260068416435114 24.311347874564813 18.928142516541556 12 22.84375718992008 24.76159173137068 31.50892877459743 20.885722304111813 13 28.78021030595243 21.441194986840078 25.77686533390305 24.001729373304443 14 21.267351585820283 21.341230619395546 27.478904527151432 29.91251326763274 15 23.49793562382591 29.647425281718455 23.19175564258607 23.66288345186957 16 22.874349904808618 27.645144733881306 26.690095798179104 22.790409563130975 17 22.184980459454444 28.038798869698166 27.169475983614973 22.606744687232414 18 22.1507039589026 27.371846297500912 28.38263962585017 22.09481011774632 19 23.516746350997046 26.944596846169212 27.133917713181923 22.404739089651823 20 23.634465095229306 26.908998050662547 27.389581790002502 22.066955064105642 21 23.4110969242633 26.363349051236995 26.867704132586628 23.357849891913077 22 23.279309746747685 27.18506790831076 26.822556511880858 22.713065833060693 23 22.79720612136947 26.949287623655728 27.01553402182389 23.23797223315091 24 23.0463770387769 26.86520748687168 27.39605429827644 22.69236117607498 25 23.068221754201442 26.77949731871288 27.07374361282265 23.07853731426303 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 207.0 1 207.0 2 277.0 3 347.0 4 347.0 5 347.0 6 785.0 7 1223.0 8 1223.0 9 1223.0 10 1839.0 11 2455.0 12 2455.0 13 2455.0 14 4761.0 15 7067.0 16 7067.0 17 7067.0 18 13428.5 19 19790.0 20 19790.0 21 19790.0 22 32110.5 23 44431.0 24 44431.0 25 44431.0 26 64053.0 27 83675.0 28 83675.0 29 83675.0 30 108406.0 31 133137.0 32 133137.0 33 133137.0 34 154712.0 35 176287.0 36 176287.0 37 176287.0 38 196338.0 39 216389.0 40 216389.0 41 216389.0 42 229890.5 43 243392.0 44 243392.0 45 243392.0 46 253852.0 47 264312.0 48 264312.0 49 264312.0 50 258587.5 51 252863.0 52 252863.0 53 252863.0 54 227566.5 55 202270.0 56 202270.0 57 202270.0 58 173986.0 59 145702.0 60 145702.0 61 145702.0 62 119558.5 63 93415.0 64 93415.0 65 93415.0 66 72208.5 67 51002.0 68 51002.0 69 51002.0 70 37473.5 71 23945.0 72 23945.0 73 23945.0 74 16900.5 75 9856.0 76 9856.0 77 9856.0 78 6860.0 79 3864.0 80 3864.0 81 3864.0 82 2632.0 83 1400.0 84 1400.0 85 1400.0 86 924.5 87 449.0 88 449.0 89 449.0 90 277.0 91 105.0 92 105.0 93 105.0 94 57.5 95 10.0 96 10.0 97 10.0 98 6.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17415092574566582 2 0.008950265347555995 3 0.005966843565037331 4 0.0018709594229354342 5 0.07524290868453855 6 0.31330985363535 7 0.027255327809789166 8 0.014360877732801713 9 0.0048543812054540995 10 0.0037924853167610154 11 0.0 12 0.0 13 0.0 14 0.0019215258938255811 15 0.0 16 0.0 17 0.021440183657422274 18 0.03200857607346297 19 0.0 20 0.0 21 0.0010113294178029373 22 1.0113294178029373E-4 23 0.006523074744828945 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1977595.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.67385959389716 #Duplication Level Percentage of deduplicated Percentage of total 1 82.21423333067287 59.74825649693221 2 12.167460866937484 17.68512685316106 3 3.102115619201221 6.763281448615946 4 1.0938402564708274 3.1797437286765344 5 0.489852216989615 1.7799725619631266 6 0.25888574755144544 1.1288535881048913 7 0.16514105082915398 0.84010062788026 8 0.10672815976529758 0.6205077837998608 9 0.07141725103913192 0.4671150547139982 >10 0.299331635861383 3.7733253317262023 >50 0.018178747860518395 0.9102166919877039 >100 0.010657219485760165 1.498950385528765 >500 0.0014618014206485674 0.6952305646282045 >1k 6.960959145945188E-4 0.9093188822812668 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3002 0.1518005456122209 No Hit GTACATGGGAAGCAGTGGTATCAAC 2161 0.10927414359360739 No Hit CCCATGTACTCTGCGTTGATACCAC 2159 0.1091730106518271 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 395 0.0 11.810461 4 TTACACG 65 7.880051E-4 11.717756 4 AGTACGT 75 2.0328841E-4 11.424813 5 TAGTACG 85 5.2076903E-5 11.200797 4 CTTACAC 215 0.0 11.070836 3 CGCCTTT 70 0.0015034757 10.846925 12 GTCCTAC 915 0.0 10.72053 1 TGGCGAG 905 0.0 10.696564 18 GGACGTG 765 0.0 10.578261 6 AGGACGT 805 0.0 10.407698 5 TTAGTAC 175 2.2737368E-10 10.336998 3 GTCTTAT 195 1.4551915E-11 10.25616 1 TGTGCGG 85 6.6705426E-4 10.046308 10 TCCTACA 1015 0.0 10.036831 2 ATGGCGA 1005 0.0 10.01047 17 GGCGAGA 570 0.0 9.987557 19 TGTAGGA 1040 0.0 9.978656 2 TATGGCG 965 0.0 9.933647 16 GACGTGA 465 0.0 9.798461 7 GTCTTAC 205 4.0017767E-11 9.755859 1 >>END_MODULE