FastQCFastQC Report
Thu 26 May 2016
SRR1513162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897848
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26310.13863070172110725No Hit
GTACATGGGAAGCAGTGGTATCAAC24990.131675455568623No Hit
CCCATGTACTCTGCGTTGATACCAC20860.10991396571274413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6250.012.3393274
ACCGTAT658.092679E-411.6805828
TGGCGAG10800.011.42522218
GGCCTAT855.088567E-511.226211
GGCGAGA7050.011.17465319
TACCGTA600.005920516311.0722197
CTACACT3000.010.7905644
GTCTTAG2750.010.756751
GGACCTG9400.010.701746
CTATTAG1252.1075175E-710.6873521
CGCAGAT803.8066285E-410.6767828
AGGACCT10000.010.6520695
TGTACCG1252.2057793E-710.6520695
TTAGGAC7350.010.6221643
TATGGCG12050.010.55511116
GTACCGT909.6165575E-510.5446926
GTCCTAC11800.010.512681
ATGGCGA11950.010.48458217
GTATAAG2100.010.4510671
AGGACGT9250.010.28191955
Produced by FastQC (version 0.11.5)