##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513162_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1897848
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.48082986624851	31.0	30.0	34.0	25.0	34.0
2	29.349697657557403	31.0	30.0	34.0	22.0	34.0
3	29.51250416260944	31.0	30.0	34.0	23.0	34.0
4	32.61987893656394	37.0	33.0	37.0	23.0	37.0
5	32.73254602054538	37.0	33.0	37.0	25.0	37.0
6	33.05590015638766	37.0	35.0	37.0	26.0	37.0
7	33.004718502219355	37.0	35.0	37.0	26.0	37.0
8	32.925263772441205	37.0	35.0	37.0	26.0	37.0
9	34.14511488802054	39.0	35.0	39.0	25.0	39.0
10	33.750813026122216	39.0	34.0	39.0	19.0	39.0
11	34.17874877229367	39.0	35.0	39.0	25.0	39.0
12	34.17330154996607	39.0	35.0	39.0	25.0	39.0
13	34.20943036534011	39.0	35.0	39.0	25.0	39.0
14	34.500731881583775	40.0	34.0	41.0	17.0	41.0
15	34.91136856060127	40.0	34.0	41.0	21.0	41.0
16	34.740328519459936	40.0	34.0	41.0	18.0	41.0
17	34.38677280793826	39.0	34.0	41.0	17.0	41.0
18	34.132205529631456	39.0	33.0	41.0	16.0	41.0
19	34.74279394345596	40.0	34.0	41.0	18.0	41.0
20	34.9543198401558	40.0	35.0	41.0	22.0	41.0
21	34.99634744194477	40.0	36.0	41.0	22.0	41.0
22	34.69539604857712	40.0	35.0	41.0	18.0	41.0
23	34.24241878169379	40.0	34.0	41.0	10.0	41.0
24	34.477558265993906	40.0	34.0	41.0	16.0	41.0
25	34.10853134708364	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9118188775248548
1101	2	1.1260957061373418
1101	3	1.0362994465191022
1101	4	1.4152834957709146
1101	5	1.286162439342739
1101	6	0.9475613675371051
1101	7	0.966790410784995
1101	8	0.9596755081137402
1101	9	1.2523049836338274
1101	10	1.6563853701218036
1101	11	1.2366980185950425
1101	12	1.1803596069457711
1101	13	1.106737956331287
1101	14	2.1079396435438014
1101	15	1.6528327300300276
1101	16	1.7868945044253834
1101	17	2.2256704013122857
1101	18	2.1682258069317797
1101	19	1.7797354547876694
1101	20	1.3961002027195377
1101	21	1.3776656385521449
1101	22	1.5370714241030683
1101	23	1.8438037435728916
1101	24	1.5770630770420837
1101	25	1.8820922513260925
1102	1	0.6795348171084967
1102	2	0.8674444043800662
1102	3	0.763033333763488
1102	4	1.082613525298477
1102	5	1.004875650162404
1102	6	0.7441639147726562
1102	7	0.7543826965621037
1102	8	0.834260469404903
1102	9	1.106134496099422
1102	10	1.2958152420119404
1102	11	0.892462313580026
1102	12	0.8341765144719986
1102	13	0.8084899564194998
1102	14	1.7358421578508398
1102	15	1.1855818975743162
1102	16	1.3871991201898624
1102	17	1.7945746645276301
1102	18	1.9815101157713855
1102	19	1.327746436435703
1102	20	1.1007818242206682
1102	21	1.066240288008352
1102	22	1.3153809179574623
1102	23	1.5950909932767345
1102	24	1.400962128558234
1102	25	1.5964151379826106
1103	1	0.7070621704354103
1103	2	0.9337508410484787
1103	3	0.7245949667332603
1103	4	1.1809423322907762
1103	5	0.9431831251632303
1103	6	0.7462302221803299
1103	7	0.6974996876249406
1103	8	0.7632576931819344
1103	9	1.1202336564138875
1103	10	1.3509689915960266
1103	11	0.9354732704469413
1103	12	0.7606783527001539
1103	13	0.7274351858948478
1103	14	1.6663691525517237
1103	15	1.3212731934917201
1103	16	1.3206325609671552
1103	17	1.6665003957082902
1103	18	1.8574069505837443
1103	19	1.3092274583861183
1103	20	0.9150276716462216
1103	21	0.970503104357725
1103	22	1.150012454521132
1103	23	1.557790648935324
1103	24	1.3409821399772994
1103	25	1.6692095339221495
1104	1	0.5217248827242003
1104	2	0.6704328762579834
1104	3	0.6049288440094038
1104	4	1.1256194633809926
1104	5	0.920445020674407
1104	6	0.6904825598393032
1104	7	0.5948041488193709
1104	8	0.5648705267786056
1104	9	0.9829882518672193
1104	10	1.1294556218271623
1104	11	0.7362555120093504
1104	12	0.708017349945905
1104	13	0.6741620316309422
1104	14	1.6234697647032306
1104	15	1.1548715910189387
1104	16	1.2754585424385567
1104	17	1.6831683574768803
1104	18	1.7377214013310223
1104	19	1.0646737936793684
1104	20	0.9246334426203973
1104	21	0.8378099830757506
1104	22	1.1081741868494674
1104	23	1.2972998916460625
1104	24	1.104164550766363
1104	25	1.2279998223845183
1105	1	0.45128929620889124
1105	2	0.6605494053257495
1105	3	0.5966535602116743
1105	4	0.9906929927927592
1105	5	0.8418781992089848
1105	6	0.4893731687144367
1105	7	0.43381773671066526
1105	8	0.4405112216530469
1105	9	0.6958128992700381
1105	10	1.0167793232375502
1105	11	0.6050119619577501
1105	12	0.5354962801144652
1105	13	0.4183871778297714
1105	14	1.1930625579608858
1105	15	0.8880673254530649
1105	16	0.9916565128547958
1105	17	1.3347163450929784
1105	18	1.084515776976012
1105	19	0.8185201129088142
1105	20	0.6441058368412413
1105	21	0.48271022387382345
1105	22	0.6942938978917752
1105	23	0.9069146147282794
1105	24	0.7189843822077151
1105	25	0.99201771024142
1106	1	0.4873531338058399
1106	2	0.7017161914656995
1106	3	0.6520401138537366
1106	4	1.1217912041481881
1106	5	1.04589692772538
1106	6	0.6202028890605931
1106	7	0.4867932691584542
1106	8	0.5537664943112262
1106	9	0.7833300645577239
1106	10	0.7034797878878507
1106	11	0.6791707549033603
1106	12	0.6419682413187573
1106	13	0.6054678697959872
1106	14	1.6334354225559125
1106	15	1.1417257988436305
1106	16	1.1758547319831862
1106	17	1.5508443743884044
1106	18	1.5391377735904612
1106	19	1.1656092471737551
1106	20	0.8450559732998784
1106	21	0.7013061681157851
1106	22	0.9242629787823873
1106	23	1.3826292952595978
1106	24	0.875918402728324
1106	25	1.2547964572401895
1107	1	0.44580288551001246
1107	2	0.5682942284491332
1107	3	0.5463785386413527
1107	4	0.7706725009894768
1107	5	0.5845690965342598
1107	6	0.37599525672133893
1107	7	0.3680174778668359
1107	8	0.4317472009455443
1107	9	0.590035289261408
1107	10	0.9037939910546342
1107	11	0.4524882346065837
1107	12	0.3464658659643334
1107	13	0.32800538317921024
1107	14	1.1681520747857377
1107	15	0.7473166783434877
1107	16	0.9350063834328779
1107	17	1.3565627644199836
1107	18	1.400222204931147
1107	19	0.8979021232625684
1107	20	0.6345016521993116
1107	21	0.6164160047193761
1107	22	0.7528961412049782
1107	23	1.0975013274116208
1107	24	0.7871239421731957
1107	25	1.0899038189558254
1108	1	0.60286926621162
1108	2	0.6725261574391936
1108	3	0.4795465926632687
1108	4	0.9834714902020849
1108	5	0.8315138005179534
1108	6	0.582620373513997
1108	7	0.5145444888241073
1108	8	0.5020056312521746
1108	9	0.7250926238405313
1108	10	0.9993877224739833
1108	11	0.6323606120804683
1108	12	0.3274634876410545
1108	13	0.33995794412374636
1108	14	1.3706242568973437
1108	15	0.8161795425242389
1108	16	0.9438722205177399
1108	17	1.2176281258401858
1108	18	1.4422690494057946
1108	19	0.868893374430499
1108	20	0.569492733487003
1108	21	0.7737894439447217
1108	22	0.8841698182626558
1108	23	1.056393071232229
1108	24	0.798765197588132
1108	25	1.2027860494833078
1109	1	0.5720841792973417
1109	2	0.773094198613812
1109	3	0.734692683246756
1109	4	1.0343292456364352
1109	5	1.083108870744823
1109	6	0.7367668193994845
1109	7	0.6789734334761377
1109	8	0.7404455818466715
1109	9	0.8458940403336186
1109	10	1.2425058511269853
1109	11	0.9050996242398739
1109	12	0.858795524935978
1109	13	0.742742539340469
1109	14	1.758836102767006
1109	15	1.1945440714291493
1109	16	1.4326390573701318
1109	17	1.7813971517570266
1109	18	1.9603516689076628
1109	19	1.3050088964129145
1109	20	1.0521060891375669
1109	21	0.984650339844606
1109	22	1.2337564639592031
1109	23	1.656466468037209
1109	24	1.3691809809111675
1109	25	1.6014895129200326
1110	1	0.5086042212319164
1110	2	0.6365534061250422
1110	3	0.5914943318750687
1110	4	1.0037562975137888
1110	5	0.8214992092696249
1110	6	0.5568155241904478
1110	7	0.5295786704911407
1110	8	0.5332821986994958
1110	9	0.795642506922384
1110	10	1.0852064574315676
1110	11	0.7337399597336329
1110	12	0.6999720277122492
1110	13	0.558113464080229
1110	14	1.3319119046273187
1110	15	0.9306617199900842
1110	16	1.1935255073118896
1110	17	1.406358888228631
1110	18	1.1776834673723613
1110	19	0.8939157402654203
1110	20	0.7331854140857743
1110	21	0.5964827908144485
1110	22	0.9638843481011534
1110	23	-0.327888429528123
1110	24	0.5493877418365614
1110	25	0.9136063881097414
1111	1	0.4401722529462866
1111	2	0.775076478899738
1111	3	0.6897353320496471
1111	4	1.0479246681854306
1111	5	1.00658094101113
1111	6	0.6262566076783358
1111	7	0.5464141820123984
1111	8	0.5307048011515292
1111	9	0.7533407995180426
1111	10	1.071239918442842
1111	11	0.7030107219467254
1111	12	0.545159754900908
1111	13	0.35979321255008045
1111	14	1.360090320429883
1111	15	0.9477325224770397
1111	16	1.068275416133936
1111	17	1.4707799989921284
1111	18	1.7014160938677207
1111	19	1.0839599723742381
1111	20	0.7689525577009846
1111	21	0.6657220728680358
1111	22	1.0711849147008152
1111	23	1.5043829255577066
1111	24	1.2609088104248514
1111	25	1.4911290356754208
1112	1	0.09269913372213878
1112	2	0.2542640821184037
1112	3	0.16008741750591682
1112	4	0.5841074223229583
1112	5	0.3324005411376305
1112	6	0.170183836323055
1112	7	0.011718655114052012
1112	8	0.08963255687028493
1112	9	0.38364184663846146
1112	10	0.5663201215420344
1112	11	0.055409348040406314
1112	12	0.07618640727800852
1112	13	9.330979993222854E-4
1112	14	0.4859692163854703
1112	15	0.378918614750134
1112	16	0.4130314687007868
1112	17	0.8196863203667348
1112	18	0.997123954298516
1112	19	0.4696305862397949
1112	20	0.20375569554840922
1112	21	0.11297338176855476
1112	22	0.43374992049862726
1112	23	0.6627076207332223
1112	24	0.35859715154716554
1112	25	0.5932540224985701
1113	1	-0.0899970014317617
1113	2	0.023754940715949857
1113	3	0.0400133605746511
1113	4	0.2837533376203467
1113	5	0.33398119376433755
1113	6	0.02393759702659537
1113	7	0.05887672037672331
1113	8	0.10162283689805918
1113	9	0.24709012250053064
1113	10	0.6483219729653129
1113	11	0.14803374054213947
1113	12	0.04777168846292312
1113	13	-0.06081881128093869
1113	14	0.508468637815696
1113	15	0.20153375013546082
1113	16	0.24991876330851426
1113	17	0.6975294122436182
1113	18	0.48670044122283684
1113	19	0.22003327080355461
1113	20	0.056208831396595826
1113	21	-0.05668179064523571
1113	22	0.08142638428016369
1113	23	0.3596550865976056
1113	24	0.290515688922774
1113	25	0.5810739715844306
1114	1	-1.0121174249249876
1114	2	-0.4776225952159123
1114	3	-0.552836938118201
1114	4	-0.2592962867955819
1114	5	-3.7303655265290345
1114	6	-1.3497236083912796
1114	7	-0.927714040846439
1114	8	-0.5772835332444899
1114	9	-0.30958285319001533
1114	10	-0.04649141793785816
1114	11	-0.5850507806620939
1114	12	-0.6035141694657469
1114	13	-0.5528629350166696
1114	14	-6.230020745280065
1114	15	-2.4913088594345396
1114	16	-1.4094459968499962
1114	17	-0.39509317011872014
1114	18	-6.549551998594989
1114	19	-2.5649924581844417
1114	20	-1.5613282834079243
1114	21	-1.1620694998781005
1114	22	-1.0344232637946007
1114	23	-0.37820319695358506
1114	24	-0.5987928814918462
1114	25	-0.31446870304738184
1115	1	-0.20720441770259512
1115	2	0.11213510920163472
1115	3	0.030349969347025052
1115	4	-2.68730604330959
1115	5	-0.5862239139867285
1115	6	-0.16158750057181948
1115	7	-0.07581501367212695
1115	8	0.09212563492070558
1115	9	0.29466533069033574
1115	10	0.07444438494544414
1115	11	-0.056190004577935326
1115	12	0.014062586126605936
1115	13	-0.03940263713877101
1115	14	-5.2419989885183504
1115	15	-1.6817493033956055
1115	16	-0.6228404617056853
1115	17	0.31231517919189855
1115	18	0.4632342773541964
1115	19	0.14917102054067044
1115	20	0.17862202017549578
1115	21	-0.005726232672380149
1115	22	-0.9359125330119795
1115	23	-0.10209805891165757
1115	24	0.19881620743323936
1115	25	0.6475963255494008
1116	1	0.2098162353557349
1116	2	0.5453837101980454
1116	3	0.3892764626983656
1116	4	0.5362995869028708
1116	5	-0.03880656728471621
1116	6	0.24982704026911762
1116	7	0.22030857167258944
1116	8	0.35954636553066877
1116	9	0.5700054830020918
1116	10	0.6693131867054376
1116	11	0.3231752478934595
1116	12	0.2141264944146215
1116	13	0.17972470057389245
1116	14	1.0998466235987294
1116	15	0.6559189327042247
1116	16	0.7230981531270544
1116	17	1.1639433819552991
1116	18	1.0050659319729789
1116	19	0.8281126562068835
1116	20	0.4848679333437005
1116	21	0.3177749182602341
1116	22	0.48385518908390424
1116	23	-0.20831215257410207
1116	24	0.1342006198248029
1116	25	0.8883820205049133
1201	1	0.6814393240562886
1201	2	0.8754982357351082
1201	3	0.9004042563965804
1201	4	1.3756466964964602
1201	5	1.120207703525331
1201	6	0.9122184764281656
1201	7	0.8325959321993892
1201	8	0.9340192973979669
1201	9	1.0963402593368698
1201	10	1.4759620021570683
1201	11	0.9319021039794677
1201	12	0.9142887473937762
1201	13	0.8509477459166561
1201	14	1.820894290068935
1201	15	1.5354427083547648
1201	16	1.5484860867896373
1201	17	1.920757837713417
1201	18	1.302357255672753
1201	19	1.2625729533432377
1201	20	1.3509406321628816
1201	21	1.219388725683899
1201	22	1.404023081963068
1201	23	1.6592746815620245
1201	24	1.374036944168168
1201	25	1.6208919792472685
1202	1	0.5996089098982864
1202	2	0.8990751377602848
1202	3	0.7663844607388519
1202	4	1.1626039123329903
1202	5	0.9415838210221352
1202	6	0.6327553310167389
1202	7	0.6381378041205679
1202	8	0.7829200454366472
1202	9	0.9907847037813156
1202	10	1.2074845345416705
1202	11	0.8238629687851642
1202	12	0.6697257114514272
1202	13	0.7908560655663521
1202	14	1.6318183915102793
1202	15	1.1919606795279094
1202	16	1.1764212263627059
1202	17	1.711995750503057
1202	18	1.8741552852294063
1202	19	1.3234651263043773
1202	20	1.060313921708584
1202	21	1.0419553193711835
1202	22	1.2806217865790117
1202	23	1.653595973297115
1202	24	1.217642125704387
1202	25	1.615589570927618
1203	1	0.46072624084320424
1203	2	0.5454477306846037
1203	3	0.4708888643811875
1203	4	0.784442992792755
1203	5	0.7996148704324924
1203	6	0.38508403679372094
1203	7	0.43779073739418806
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2211	14	-0.10619022199613681
2211	15	-0.2504717474397964
2211	16	-6.837637497446394
2211	17	-2.0629812365149576
2211	18	-0.8736951160212243
2211	19	-0.7908351673040315
2211	20	-0.8812819501083098
2211	21	-0.7571821593850387
2211	22	-0.6352623322818403
2211	23	-0.2743130006484691
2211	24	-0.7292988237819884
2211	25	-8.21409307872062
2212	1	-1.6135969115744082
2212	2	-4.120927750511726
2212	3	-1.967521178344814
2212	4	-3.875491860627438
2212	5	-1.5172026768410767
2212	6	-1.2820952009066886
2212	7	-1.2196260315046636
2212	8	-1.0966844752439187
2212	9	-1.1875307917980251
2212	10	-0.49488705769850583
2212	11	-1.113747681450441
2212	12	-1.2347409477271256
2212	13	-1.5112523730017031
2212	14	-6.593885624792414
2212	15	-5.138925645573853
2212	16	-2.954385403826155
2212	17	-8.036653447466595
2212	18	-3.543590471952495
2212	19	-2.3005856217045917
2212	20	-2.105365918687646
2212	21	-1.6976129374496836
2212	22	-1.5163867124288117
2212	23	-1.1159295477952966
2212	24	-1.4652250726182814
2212	25	-1.296041607433878
2213	1	-0.2847408774599032
2213	2	-0.25059551991445517
2213	3	-0.2579244066302593
2213	4	0.1674888762942217
2213	5	0.0010659250726163805
2213	6	-0.27302471095055836
2213	7	-0.5428108364306183
2213	8	-0.36847344102871205
2213	9	-0.18708708274650832
2213	10	-4.123408561051761
2213	11	-1.5611941284390838
2213	12	-1.1412336640511853
2213	13	-0.9043244274933286
2213	14	0.03825487388737514
2213	15	-0.3631709638921663
2213	16	-0.20501890290555735
2213	17	-0.012181080703861369
2213	18	0.170550660597371
2213	19	-0.400197773778288
2213	20	-0.7292967399309873
2213	21	-0.7486113888139343
2213	22	-0.8201117060954104
2213	23	-1.1130478769222591
2213	24	-0.7687719890735636
2213	25	-0.39147604693055627
2214	1	-0.8711736999298338
2214	2	-0.5799893900268067
2214	3	-0.526124251386058
2214	4	-0.19665416517155165
2214	5	-3.5362745210748727
2214	6	-1.3534147743752811
2214	7	-1.0975216204396467
2214	8	-0.8569698198469347
2214	9	-0.6730997263952503
2214	10	-4.156304824673537
2214	11	-1.625299896670974
2214	12	-1.1313378677966242
2214	13	-0.81924327056484
2214	14	0.009443180286694997
2214	15	-0.34583708196336005
2214	16	-0.3482324320785892
2214	17	-0.06683164252311968
2214	18	0.3252737256315257
2214	19	-0.43302026521759274
2214	20	-0.7843773527089724
2214	21	-0.8041779731554328
2214	22	-0.47372711299631476
2214	23	-0.1486059613318318
2214	24	-0.5841735560119261
2214	25	-0.2053959329213484
2215	1	-1.6225482266803155
2215	2	-4.204251639872503
2215	3	-2.2157330135686273
2215	4	-1.1907206310446519
2215	5	-1.2281662874382242
2215	6	-1.3973660484293546
2215	7	-1.5404971734548631
2215	8	-1.7550090658897837
2215	9	-7.5169265309020155
2215	10	-3.2179805440503735
2215	11	-2.444383853623229
2215	12	-2.7784424538630894
2215	13	-2.081232770680842
2215	14	-7.458372323377837
2215	15	-4.173294786336918
2215	16	-8.835444535062965
2215	17	-3.9473203864188875
2215	18	-2.3560605551305187
2215	19	-2.758151365779291
2215	20	-2.511763658417749
2215	21	-2.597033507656178
2215	22	-9.027819342494755
2215	23	-10.26300126176972
2215	24	-6.202101223427114
2215	25	-4.344141798071217
2216	1	-1.400789371204322
2216	2	-1.293646154153965
2216	3	-1.1589956128437144
2216	4	-6.490461535303261
2216	5	-2.82343194091219
2216	6	-1.795581751318874
2216	7	-1.4769960715415529
2216	8	-7.115111929924751
2216	9	-3.1307334943326275
2216	10	-1.5537306259398136
2216	11	-1.356950739394648
2216	12	-1.4373834181081762
2216	13	-1.3286384775525448
2216	14	-0.4788301186980277
2216	15	-1.1714856076161055
2216	16	-0.8748490001092861
2216	17	-0.6245480187626455
2216	18	-6.97646941411136
2216	19	-3.8996448524915905
2216	20	-3.1159397097174626
2216	21	-2.3805618064474814
2216	22	-1.8911599166773811
2216	23	-1.2137834069215927
2216	24	-1.74924088123732
2216	25	-0.9539739054590441
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90128.0
3	2.0
4	732.0
5	4307.0
6	5432.0
7	4865.0
8	4371.0
9	4183.0
10	3802.0
11	3575.0
12	3601.0
13	3569.0
14	3434.0
15	3541.0
16	3584.0
17	3843.0
18	4298.0
19	5345.0
20	6852.0
21	8687.0
22	10689.0
23	13095.0
24	14741.0
25	16638.0
26	18766.0
27	21266.0
28	23689.0
29	28368.0
30	34040.0
31	40901.0
32	52107.0
33	68488.0
34	91140.0
35	124712.0
36	180938.0
37	267236.0
38	722883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58695881620333	18.530228189052835	12.358833090579022	25.52397990416482
2	14.57228416154809	20.255567937552485	41.21979687656792	23.952351024331502
3	18.379541727513512	25.04754636463744	29.612155813701698	26.96075609414735
4	11.552219836040786	16.751469510628375	38.919218418078025	32.777092235252816
5	12.738980655512433	38.081109642352224	36.07446695582967	13.105442746305673
6	31.80258754710021	37.11438433769862	18.22966456746858	12.853363547732602
7	27.9476585473824	31.63279825458225	22.464032378764085	17.955510819271268
8	25.352005050702008	36.18339992769655	20.76110453540814	17.703490486193306
9	26.685610422297838	14.909775620841012	19.99423466190824	38.410379294952904
10	14.08577188105245	28.753158803146288	35.36736979406843	21.79369952173283
11	34.43281452816099	22.495218711347185	24.42301743187487	18.648949328616954
12	23.121470757820596	24.853243608757843	31.572625571714834	20.45266006170673
13	28.790716844140213	21.761362558317504	25.740497909740416	23.707422687801866
14	20.722240082638095	21.53686829026631	28.450324913172025	29.29056671392357
15	23.400520587891112	29.986358024886588	23.411115784945967	23.202005602276333
16	22.358425832917874	27.827557093965382	27.445472588467055	22.36854448464969
17	21.83766995695531	28.001558169372377	27.953221183091227	22.207550690581087
18	21.659499399931377	27.305151629988778	29.365729835770416	21.669619134309432
19	23.43971113501571	27.002983624062317	27.497724466191652	22.05958077473032
20	23.78604215298681	26.99091933019453	27.63176827188987	21.591270244928786
21	23.37882205110879	26.579881270071848	26.984572910116604	23.05672376870276
22	23.357636529543644	27.36600339376713	26.97870909279723	22.297650983891995
23	22.507013792518237	27.17140322758314	27.62124518995875	22.70033778993988
24	23.04481885893491	27.006078714903474	27.595025058686407	22.354077367475213
25	22.94457747752069	26.784837989256737	27.59751400624819	22.67307052697438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	208.0
1	208.0
2	340.5
3	473.0
4	473.0
5	473.0
6	945.5
7	1418.0
8	1418.0
9	1418.0
10	2305.0
11	3192.0
12	3192.0
13	3192.0
14	5640.5
15	8089.0
16	8089.0
17	8089.0
18	14907.5
19	21726.0
20	21726.0
21	21726.0
22	34069.0
23	46412.0
24	46412.0
25	46412.0
26	66044.0
27	85676.0
28	85676.0
29	85676.0
30	109528.5
31	133381.0
32	133381.0
33	133381.0
34	154272.0
35	175163.0
36	175163.0
37	175163.0
38	192722.0
39	210281.0
40	210281.0
41	210281.0
42	222910.5
43	235540.0
44	235540.0
45	235540.0
46	244198.0
47	252856.0
48	252856.0
49	252856.0
50	244702.0
51	236548.0
52	236548.0
53	236548.0
54	211694.0
55	186840.0
56	186840.0
57	186840.0
58	159995.0
59	133150.0
60	133150.0
61	133150.0
62	108810.5
63	84471.0
64	84471.0
65	84471.0
66	65517.5
67	46564.0
68	46564.0
69	46564.0
70	34215.0
71	21866.0
72	21866.0
73	21866.0
74	15343.5
75	8821.0
76	8821.0
77	8821.0
78	6134.0
79	3447.0
80	3447.0
81	3447.0
82	2334.5
83	1222.0
84	1222.0
85	1222.0
86	788.0
87	354.0
88	354.0
89	354.0
90	234.0
91	114.0
92	114.0
93	114.0
94	69.0
95	24.0
96	24.0
97	24.0
98	18.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15428000556419694
2	0.23278998107329985
3	0.233580349954264
4	0.33732943839548796
5	0.22852198911609362
6	0.01570199510182059
7	0.016228907689130004
8	0.015280465031973056
9	0.015860068878013413
10	0.019653839506641207
11	0.018283866779636726
12	0.025607951743237607
13	0.025081039155928186
14	0.022024946149533577
15	0.040150739152977476
16	0.01907423566060085
17	0.039518444048206175
18	0.029770561182981988
19	0.02587140803689231
20	0.02745214579882056
21	0.03166744649729589
22	0.04431334859272186
23	0.027135998246434912
24	0.04747482411657836
25	0.0347235395036905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1897848.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.20839920131029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.04801039798475	65.5758112688812
2	9.665269683827727	14.73149460906931
3	2.228214919478209	5.094260762697324
4	0.80711186254299	2.4603481208315703
5	0.37698071298779345	1.4364548333284168
6	0.22710601174826348	1.0384431362597475
7	0.13428779995184426	0.716370078661709
8	0.10133143934652723	0.6177845425090805
9	0.0685013071983702	0.46983374683064866
>10	0.31687501779207283	4.1137729001067544
>50	0.015545116490362857	0.8101461019731826
>100	0.008669266037541017	1.2982415407781058
>500	0.0013976430756977934	0.690571469115222
>1k	6.9882153784889E-4	0.9464668889577874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2631	0.13863070172110725	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2499	0.131675455568623	No Hit
CCCATGTACTCTGCGTTGATACCAC	2086	0.10991396571274413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.269125873094157E-5	0.0
3	0.0	0.0	0.0	5.269125873094157E-5	0.0
4	0.0	0.0	0.0	5.269125873094157E-5	0.0
5	0.0	0.0	0.0	5.269125873094157E-5	0.0
6	5.269125873094157E-5	0.0	0.0	5.269125873094157E-5	0.0
7	5.269125873094157E-5	0.0	0.0	5.269125873094157E-5	0.0
8	5.269125873094157E-5	0.0	0.0	5.269125873094157E-5	0.0
9	5.269125873094157E-5	0.0	0.0	5.269125873094157E-5	0.0
10	5.269125873094157E-5	0.0	0.0	1.0538251746188315E-4	0.0
11	5.269125873094157E-5	0.0	0.0	1.0538251746188315E-4	0.0
12	5.269125873094157E-5	0.0	0.0	1.0538251746188315E-4	0.0
13	5.269125873094157E-5	5.269125873094157E-5	0.0	1.0538251746188315E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	625	0.0	12.339327	4
ACCGTAT	65	8.092679E-4	11.680582	8
TGGCGAG	1080	0.0	11.425222	18
GGCCTAT	85	5.088567E-5	11.22621	1
GGCGAGA	705	0.0	11.174653	19
TACCGTA	60	0.0059205163	11.072219	7
CTACACT	300	0.0	10.790564	4
GTCTTAG	275	0.0	10.75675	1
GGACCTG	940	0.0	10.70174	6
CTATTAG	125	2.1075175E-7	10.687352	1
CGCAGAT	80	3.8066285E-4	10.676782	8
AGGACCT	1000	0.0	10.652069	5
TGTACCG	125	2.2057793E-7	10.652069	5
TTAGGAC	735	0.0	10.622164	3
TATGGCG	1205	0.0	10.555111	16
GTACCGT	90	9.6165575E-5	10.544692	6
GTCCTAC	1180	0.0	10.51268	1
ATGGCGA	1195	0.0	10.484582	17
GTATAAG	210	0.0	10.451067	1
AGGACGT	925	0.0	10.2819195	5
>>END_MODULE