FastQCFastQC Report
Thu 26 May 2016
SRR1513162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897848
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38870.20481092268716988No Hit
GTACATGGGAAGCAGTGGTATCAAC27780.1463763167545557No Hit
TATCAACGCAGAGTACTTTTTTTTT26620.14026413074176647No Hit
CCCATGTACTCTGCGTTGATACCAC24600.12962049647811627No Hit
GGTATCAACGCAGAGTACTTTTTTT23420.12340292794786516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC400.005208111414.279343
GGCGAGG4200.011.74854619
AGGACGT10250.011.3299995
TAGGACC4800.011.3038794
GTATTAA2050.011.1483861
GGACGTG10300.010.9965836
TTAGGAC7650.010.9506043
TGGCGAG10850.010.847383518
CAGGACT3600.010.8411474
GACGTGA6100.010.7370927
TAGGACG11100.010.7196594
TGCTCGA803.8131655E-410.674810
GTCCTAC11600.010.5897661
AGGACTA2100.010.4256325
TCTATAC1658.967618E-1010.38497453
AGGACCT8550.010.3540615
TCCTACA13300.010.2361282
GATATAG1409.3587005E-810.2027651
TGTAGGA12450.010.1702972
CGTGAAA6850.010.1120579
Produced by FastQC (version 0.11.5)