##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513162_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1897848
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.40586970084011	31.0	31.0	34.0	26.0	34.0
2	30.525124772900675	31.0	31.0	34.0	27.0	34.0
3	30.61790301436153	33.0	31.0	34.0	27.0	34.0
4	33.98704374639065	37.0	35.0	37.0	30.0	37.0
5	33.70943984976668	37.0	35.0	37.0	28.0	37.0
6	33.6176137393511	37.0	35.0	37.0	28.0	37.0
7	33.456127677242854	37.0	35.0	37.0	27.0	37.0
8	33.41688164700229	37.0	35.0	37.0	27.0	37.0
9	34.924496060801495	39.0	35.0	39.0	27.0	39.0
10	34.75449087598164	39.0	35.0	39.0	27.0	39.0
11	34.76561821600044	39.0	35.0	39.0	27.0	39.0
12	34.70109408129629	39.0	35.0	39.0	27.0	39.0
13	34.66577144218083	39.0	35.0	39.0	27.0	39.0
14	35.79209241203721	40.0	36.0	41.0	27.0	41.0
15	35.763395698707164	40.0	36.0	41.0	27.0	41.0
16	35.718540683974695	40.0	36.0	41.0	27.0	41.0
17	35.657989470178855	40.0	36.0	41.0	27.0	41.0
18	35.608417007052196	40.0	36.0	41.0	26.0	41.0
19	35.68652389443201	40.0	36.0	41.0	26.0	41.0
20	35.647449637694905	40.0	36.0	41.0	26.0	41.0
21	35.590441384136135	40.0	36.0	41.0	26.0	41.0
22	35.533880479363994	40.0	36.0	41.0	25.0	41.0
23	35.45102400192218	40.0	36.0	41.0	25.0	41.0
24	35.38625221830199	40.0	36.0	41.0	25.0	41.0
25	35.32734075647787	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9769541209012651
1101	2	0.8590126553276924
1101	3	0.9007518315756791
1101	4	1.01295642955791
1101	5	1.0223598610637765
1101	6	0.8666999760647656
1101	7	0.9994100435638558
1101	8	1.0968237823757363
1101	9	1.1084879612206038
1101	10	1.1454172670737819
1101	11	1.174049381877765
1101	12	1.2556631311343907
1101	13	1.2569639239284953
1101	14	1.3553719944709215
1101	15	1.2829680065261684
1101	16	1.3410844302090865
1101	17	1.3938933012416115
1101	18	1.4557086391439285
1101	19	1.5251987619570215
1101	20	1.476945841635981
1101	21	1.394506370818391
1101	22	1.5701211208538126
1101	23	1.423164091514721
1101	24	1.3898939996067625
1101	25	1.4634336214872263
1102	1	0.9083336309777579
1102	2	0.8944231298809591
1102	3	0.8437191294334241
1102	4	0.9506977584730976
1102	5	1.0592387137591714
1102	6	0.9131882032027434
1102	7	0.9660931132276573
1102	8	1.0583345027361304
1102	9	1.030803157783069
1102	10	1.167596771355889
1102	11	1.1482875235594463
1102	12	1.1083786307342933
1102	13	1.2014711648499699
1102	14	1.4293093688944865
1102	15	1.3176900740994526
1102	16	1.3221659945447612
1102	17	1.3424975798726067
1102	18	1.3552460832730233
1102	19	1.2443247142731622
1102	20	1.319761274838342
1102	21	1.3090928801604278
1102	22	1.2723394820776406
1102	23	1.3128127120738355
1102	24	1.3194581173442472
1102	25	1.4288240902936806
1103	1	0.6067403560574576
1103	2	0.6922557943688261
1103	3	0.6323554744342594
1103	4	0.5824324080057011
1103	5	0.7268302486311313
1103	6	0.5425357886483084
1103	7	0.7489790343290679
1103	8	0.6854674321584397
1103	9	0.8992209566823277
1103	10	0.8429272489154371
1103	11	0.8355239755007489
1103	12	0.8391486582289573
1103	13	0.8986739465105842
1103	14	0.9788006248494696
1103	15	1.037707578157196
1103	16	1.0112699128039182
1103	17	1.100055159634529
1103	18	1.057501349055478
1103	19	0.9972668719072146
1103	20	0.9917999235906834
1103	21	0.9882493494331683
1103	22	1.0992081243954317
1103	23	1.0154420321473552
1103	24	1.0880647019944476
1103	25	1.1044538544420348
1104	1	0.5907734761306713
1104	2	0.6102266286734839
1104	3	0.6762007183882908
1104	4	0.7758204809803715
1104	5	0.7199187213605995
1104	6	0.6636701252539297
1104	7	0.6717633307865327
1104	8	0.7130379936282907
1104	9	0.7716089519508884
1104	10	0.8018599985895065
1104	11	0.9148942969450502
1104	12	0.8216859355793531
1104	13	0.9049880000932689
1104	14	0.809412949039384
1104	15	0.7840221497806468
1104	16	0.8192011169896887
1104	17	0.9777608434051785
1104	18	0.9444165026113964
1104	19	0.9700813874095857
1104	20	1.0265738419647263
1104	21	0.9377766372307121
1104	22	0.8689700846617043
1104	23	0.9202157778411362
1104	24	0.7340287326969985
1104	25	0.7120366762101114
1105	1	0.505236410427198
1105	2	0.518611561594021
1105	3	0.4526885064859876
1105	4	0.5264826756828143
1105	5	0.5909154533867422
1105	6	0.5694277791927007
1105	7	0.5389115936004316
1105	8	0.6399944779764155
1105	9	0.6121124779226861
1105	10	0.6204766996558959
1105	11	0.5845214899275035
1105	12	0.5676310680182937
1105	13	0.6439026887757606
1105	14	0.7468935991941805
1105	15	0.9086437528078193
1105	16	0.7259413130681196
1105	17	0.7832927491499291
1105	18	0.7033023815243666
1105	19	0.7706812326108263
1105	20	0.6650821838979937
1105	21	0.7308751924422694
1105	22	0.6774324169630432
1105	23	0.7245630430630143
1105	24	0.7623225063468055
1105	25	0.8400875018035094
1106	1	0.6013835050673109
1106	2	0.5626285411943179
1106	3	0.5544218766689113
1106	4	0.5953506735409348
1106	5	0.6015857848603261
1106	6	0.5612504088715937
1106	7	0.5886438582956401
1106	8	0.7024098390736171
1106	9	0.7489259743730301
1106	10	0.7418785746666927
1106	11	0.7129196771003947
1106	12	0.7510057851688998
1106	13	0.6549208602299927
1106	14	0.7170144471377995
1106	15	0.7973967108511104
1106	16	0.8485803842857109
1106	17	0.6623788433236655
1106	18	0.8413178799438654
1106	19	0.8850913296864675
1106	20	0.7844315655158027
1106	21	0.8974788837348271
1106	22	0.8649404058390715
1106	23	0.7391820384510623
1106	24	0.9158497030717996
1106	25	0.9236804016359557
1107	1	0.41912877453418673
1107	2	0.4242414149158513
1107	3	0.36546676360419283
1107	4	0.6230357386681504
1107	5	0.46890725666543176
1107	6	0.49883459541358377
1107	7	0.4037519732380517
1107	8	0.41647204484441147
1107	9	0.5798271025991255
1107	10	0.5473673381373487
1107	11	0.6181183406608923
1107	12	0.6703208868276107
1107	13	0.6327422056006071
1107	14	0.6953049451821016
1107	15	0.5959647191581254
1107	16	0.6622322966746808
1107	17	0.6106816620058453
1107	18	0.7606481537417977
1107	19	0.5459540971492203
1107	20	0.7356009500757281
1107	21	0.6826978844181113
1107	22	0.7407283617430238
1107	23	0.6282839231320452
1107	24	0.5314079248109991
1107	25	0.5392947925714111
1108	1	0.3337934734214727
1108	2	0.4842271874462156
1108	3	0.42706469955934523
1108	4	0.5612420157428133
1108	5	0.40756053110695234
1108	6	0.48999636386682255
1108	7	0.38044759032799647
1108	8	0.49466791675471455
1108	9	0.4908502063110092
1108	10	0.5909038199176919
1108	11	0.5987241760469502
1108	12	0.5133496390572887
1108	13	0.6080252685412333
1108	14	0.5102168198104877
1108	15	0.5785851217742817
1108	16	0.49054623534791375
1108	17	0.330045885228472
1108	18	0.5481670529178757
1108	19	0.5108349686348248
1108	20	0.48010638624323576
1108	21	0.5270812191671155
1108	22	0.4188354712308353
1108	23	0.47905581644725714
1108	24	0.4973000083920738
1108	25	0.4265495279829423
1109	1	0.38988877602595196
1109	2	0.40279514779383874
1109	3	0.3541843243536995
1109	4	0.4765318520849817
1109	5	0.4352890315472919
1109	6	0.3865080265286238
1109	7	0.49108048674651883
1109	8	0.5239559151247022
1109	9	0.388992406253422
1109	10	0.5704152291531841
1109	11	0.6113789681616026
1109	12	0.5079478068103498
1109	13	0.6109010467115468
1109	14	0.7286687176451636
1109	15	0.7289288048920497
1109	16	0.6621596263602925
1109	17	0.6523807834622346
1109	18	0.7144323911030739
1109	19	0.6550992619627252
1109	20	0.5659285525414077
1109	21	0.5660455138421341
1109	22	0.6093449086244362
1109	23	0.6594314641156487
1109	24	0.6674017872595215
1109	25	0.677739200313674
1110	1	0.4027243993413805
1110	2	0.42643983564660104
1110	3	0.34969377138147095
1110	4	0.37625635468057794
1110	5	0.3675207686723141
1110	6	0.432894313223386
1110	7	0.49835712886480366
1110	8	0.4576629372560177
1110	9	0.6533529646214404
1110	10	0.6908900069833095
1110	11	0.6253873089402049
1110	12	0.6580819289937736
1110	13	0.5875287769410349
1110	14	0.6821131218784089
1110	15	0.6036712067182677
1110	16	0.6323935250192605
1110	17	0.6845176409849998
1110	18	0.6304416797120638
1110	19	0.5520478682904866
1110	20	0.5746594501487934
1110	21	0.6206523031788365
1110	22	0.5486909528703237
1110	23	0.6640534515702541
1110	24	0.6207967292100065
1110	25	0.6995987396621004
1111	1	0.2166875677708191
1111	2	0.22518017553725755
1111	3	0.14606478094176012
1111	4	0.39028468725625487
1111	5	0.2187295895123995
1111	6	0.22810096739881658
1111	7	0.3842064406657357
1111	8	0.20720996433630035
1111	9	0.27796567439553854
1111	10	0.3911659391158011
1111	11	0.25433583272166516
1111	12	0.2689158572164203
1111	13	0.27994223134947305
1111	14	0.4304823977612813
1111	15	0.38080550535673297
1111	16	0.33317298846079524
1111	17	0.4241493900978526
1111	18	0.3098623842799455
1111	19	0.35953318907266407
1111	20	0.42029539690432927
1111	21	0.18502922936704636
1111	22	0.2679049989415532
1111	23	0.20720633322559934
1111	24	0.15615000703570558
1111	25	0.2682064739721497
1112	1	-0.4475254228472636
1112	2	-0.34347780612056766
1112	3	-0.24222495165601288
1112	4	-1.114094858757312
1112	5	-0.45849729829110686
1112	6	-0.45276844122411575
1112	7	-0.39081288538226033
1112	8	-0.3648845400384175
1112	9	-0.34935652596286815
1112	10	-0.2701604426966284
1112	11	-0.2150833316903018
1112	12	-0.24653795352886476
1112	13	-0.32145407561844763
1112	14	-0.3577801026779497
1112	15	-0.30812593988027714
1112	16	-0.3375234520325492
1112	17	-0.2761760781195832
1112	18	-0.3146263539048064
1112	19	-0.300960854990727
1112	20	-0.3619290312203418
1112	21	-0.33300241264426944
1112	22	-0.34079435802812696
1112	23	-0.3801658513205908
1112	24	-0.3334033855641749
1112	25	-0.30470894821415584
1113	1	-0.69793736452743
1113	2	-0.588421106282567
1113	3	-0.5961499690131049
1113	4	-1.5248331137908693
1113	5	-0.7233086845442926
1113	6	-0.5263208596457716
1113	7	-0.4709795134776087
1113	8	-0.40055452383846557
1113	9	-0.39320285783049513
1113	10	-0.4189130462606201
1113	11	-0.43464593294000764
1113	12	-0.4412845399671852
1113	13	-0.3521136451625324
1113	14	-0.36350794345554505
1113	15	-0.3770822000052476
1113	16	-0.4487569627939436
1113	17	-0.4565371056595069
1113	18	-0.3431336438839807
1113	19	-0.2886812900751963
1113	20	-0.3315217180983794
1113	21	-0.37872099630546074
1113	22	-0.36454580445256113
1113	23	-0.4478099151946964
1113	24	-0.44938348276389917
1113	25	-0.4209991588135509
1114	1	-0.811451885140194
1114	2	-0.8018474977180681
1114	3	-0.7492569661148991
1114	4	-1.5069943487822925
1114	5	-0.9570905500437874
1114	6	-0.9134658291850215
1114	7	-0.7864878827920947
1114	8	-0.851328759362211
1114	9	-0.8448755377855406
1114	10	-0.6878271055260115
1114	11	-0.6392795166627252
1114	12	-0.8788695639217607
1114	13	-0.7113311290449502
1114	14	-0.8059992192584673
1114	15	-0.9895078225284593
1114	16	-0.866029098595682
1114	17	-0.8498812826276279
1114	18	-0.8901135930174746
1114	19	-1.0349029734016355
1114	20	-0.9596272365226994
1114	21	-0.9969944924904723
1114	22	-0.9562409603051734
1114	23	-0.9275325153011593
1114	24	-0.9767449306848803
1114	25	-0.9450850404010609
1115	1	-0.37967455586866805
1115	2	-0.2872765155915822
1115	3	-0.2446503980835324
1115	4	-0.12988043240285663
1115	5	-0.3204979710787299
1115	6	-0.3613530701440979
1115	7	-0.4198234406202417
1115	8	-0.4917258256744006
1115	9	-0.5201705989862049
1115	10	-0.5075315603474237
1115	11	-0.5163184615547749
1115	12	-0.4801830980488191
1115	13	-0.5807246656249632
1115	14	-0.5246595443112696
1115	15	-0.6283178019729263
1115	16	-0.6414110353370646
1115	17	-0.6170670982867463
1115	18	-0.7813860581605425
1115	19	-0.7551119075740189
1115	20	-0.6206303393923562
1115	21	-0.7018998642847194
1115	22	-0.6430317297338775
1115	23	-0.7075506599642694
1115	24	-0.6819771228523734
1115	25	-0.6724213762011786
1116	1	-1.553495225801658
1116	2	-1.3676122405489792
1116	3	-1.3092260430119573
1116	4	-1.2641416925360645
1116	5	-1.5383579140908523
1116	6	-1.5685355269600052
1116	7	-1.6607009139809428
1116	8	-1.6198789973251522
1116	9	-1.7113674567090342
1116	10	-1.8667389988413063
1116	11	-1.8867488955432137
1116	12	-1.8424479467401156
1116	13	-2.011389109862961
1116	14	-2.229259190751236
1116	15	-2.0118545961375247
1116	16	-2.2199053578665797
1116	17	-2.1821669347910344
1116	18	-2.2840860038115736
1116	19	-2.4513767733194953
1116	20	-2.2398823521219526
1116	21	-2.391500809983768
1116	22	-2.3289561536953585
1116	23	-2.6574699443350553
1116	24	-2.4791728774391615
1116	25	-2.374544127686349
1201	1	0.6725000556513692
1201	2	0.728507830042922
1201	3	0.6702950227767133
1201	4	0.7431563041985498
1201	5	0.7405186279899212
1201	6	0.5800411180955152
1201	7	0.7203861132328413
1201	8	0.7387343832952737
1201	9	0.8171894063548777
1201	10	0.8115394259159885
1201	11	0.9145636091143601
1201	12	0.97003638687098
1201	13	0.9505373309367187
1201	14	1.1020366651925073
1201	15	1.1639591245705603
1201	16	0.9826542760310844
1201	17	1.1326783659697597
1201	18	1.1987893628666129
1201	19	1.1670001549165931
1201	20	1.012327032129683
1201	21	1.1022724355500415
1201	22	0.9907643579265653
1201	23	1.080066384750566
1201	24	0.9089703760209957
1201	25	0.9765519267547731
1202	1	0.4020133743888259
1202	2	0.5143703551295822
1202	3	0.47567269108541765
1202	4	0.4574328960613485
1202	5	0.6188095420566952
1202	6	0.40468078239035066
1202	7	0.5048825554879102
1202	8	0.46532396548830235
1202	9	0.5269777661432329
1202	10	0.47844327557846356
1202	11	0.6093934759269857
1202	12	0.6080586455828936
1202	13	0.5821556487619333
1202	14	0.5918286739500331
1202	15	0.6768422663330114
1202	16	0.7405041209498933
1202	17	0.6034136332567499
1202	18	0.5949123331275104
1202	19	0.6899886606637153
1202	20	0.6197754904402117
1202	21	0.6477978871099737
1202	22	0.6976381035934125
1202	23	0.6177237465458632
1202	24	0.695279899830517
1202	25	0.67417325497955
1203	1	0.5456542163055325
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2211	3	-0.4332872072637137
2211	4	-1.4108992187811467
2211	5	-0.7991606969697074
2211	6	-0.5786676091485319
2211	7	-0.6838652935299052
2211	8	-0.5209970116030078
2211	9	-0.5112069698379997
2211	10	-0.4017930158675185
2211	11	-0.5715498594602266
2211	12	-0.4246767226833512
2211	13	-0.45408226875741065
2211	14	-0.477989361204223
2211	15	-0.47991322778364065
2211	16	-0.47245061823453227
2211	17	-0.5306844227980818
2211	18	-0.6271055302674284
2211	19	-0.5749836120185705
2211	20	-0.6634407294287499
2211	21	-0.5610650640641879
2211	22	-0.4685033882011993
2211	23	-0.5187927743807847
2211	24	-0.4095282969413887
2211	25	-0.4563874530008718
2212	1	-0.3699703871040896
2212	2	-0.6209606996780508
2212	3	-0.6434284144809261
2212	4	-0.3821621040188816
2212	5	-0.5121382925415929
2212	6	-0.48624897376461007
2212	7	-0.5645771771864716
2212	8	-0.5718069106743613
2212	9	-0.7056110348494755
2212	10	-0.6205495732739834
2212	11	-0.6958005999061996
2212	12	-0.6940588856933516
2212	13	-0.5300772529351931
2212	14	-0.674290825630095
2212	15	-0.5933149305455103
2212	16	-0.6753412602217779
2212	17	-0.6614601653023229
2212	18	-0.6010253657754774
2212	19	-0.5746781450678995
2212	20	-0.6358146530641235
2212	21	-0.6068519633952008
2212	22	-0.6267506792138775
2212	23	-0.7500147899560119
2212	24	-0.8340515721719655
2212	25	-0.8498972830413436
2213	1	-0.5886371187830726
2213	2	-0.5876154238650706
2213	3	-0.6504962777981866
2213	4	-0.41165381017876257
2213	5	-0.7764820409184949
2213	6	-0.8429771654011304
2213	7	-0.9168259849701457
2213	8	-0.9715100093238362
2213	9	-1.0021902564188636
2213	10	-0.9980868290405596
2213	11	-0.9671699182626767
2213	12	-0.9513819400143149
2213	13	-0.9808061505206638
2213	14	-1.0833503977571937
2213	15	-1.0518881292892175
2213	16	-1.0605641376315162
2213	17	-1.1257311988179382
2213	18	-1.121271260724761
2213	19	-1.089806440158796
2213	20	-1.1225288211202482
2213	21	-1.059689273962917
2213	22	-1.2035068332125078
2213	23	-0.9809850669614733
2213	24	-0.9942525874240644
2213	25	-1.0575300115128172
2214	1	-0.6936797808275408
2214	2	-0.7716195929612368
2214	3	-0.8139880162816056
2214	4	-0.6049388639561002
2214	5	-0.8211879834936298
2214	6	-0.8212421167964479
2214	7	-1.0010977986839862
2214	8	-1.01941041644735
2214	9	-1.2447088860457285
2214	10	-1.2525646176989582
2214	11	-1.2550219841741423
2214	12	-1.2907580682394055
2214	13	-1.32243727713373
2214	14	-1.4834158678806446
2214	15	-1.300563294884114
2214	16	-1.4691553491922846
2214	17	-1.4679136810862659
2214	18	-1.5971161404083531
2214	19	-1.407843265597684
2214	20	-1.4754713956059575
2214	21	-1.4354443365520595
2214	22	-1.507853004124371
2214	23	-1.4925843984091358
2214	24	-1.5650386095957245
2214	25	-1.4564806183481593
2215	1	-0.8605205424527611
2215	2	-0.7953200336704072
2215	3	-0.8007181327438495
2215	4	-0.6413332860264873
2215	5	-0.9598553010052626
2215	6	-0.9321443240761482
2215	7	-0.9293816420722862
2215	8	-0.8441650575313133
2215	9	-1.1408174539082694
2215	10	-1.1556601687379597
2215	11	-1.1444982854409886
2215	12	-1.0670206987565507
2215	13	-1.1408231846401264
2215	14	-1.3865780136564112
2215	15	-1.422498713243634
2215	16	-1.3855360379480643
2215	17	-1.4170330254571013
2215	18	-1.4859132891973914
2215	19	-1.3885253277532286
2215	20	-1.326556343396824
2215	21	-1.3815640718396835
2215	22	-1.462085685906949
2215	23	-1.3199002616852482
2215	24	-1.39476816210869
2215	25	-1.4934559514299934
2216	1	-2.106092264164353
2216	2	-1.8368963776485216
2216	3	-1.95474394534736
2216	4	-1.9128309315759182
2216	5	-2.2336958395756135
2216	6	-2.356910622047714
2216	7	-2.5293443572423087
2216	8	-2.615307801863935
2216	9	-2.7743705309497457
2216	10	-2.922832191434349
2216	11	-2.7753675688487327
2216	12	-2.835651227327272
2216	13	-2.8688280948116898
2216	14	-3.257749529700071
2216	15	-3.2645717122049547
2216	16	-3.366356150107002
2216	17	-3.4425214539109135
2216	18	-3.36493867426568
2216	19	-3.5754595130749927
2216	20	-3.376121300467588
2216	21	-3.9111033730385323
2216	22	-3.597337158366443
2216	23	-3.53915009616804
2216	24	-3.5290362095919754
2216	25	-3.597215273342215
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59440.0
3	0.0
4	930.0
5	8191.0
6	9017.0
7	6910.0
8	5592.0
9	4829.0
10	4071.0
11	3785.0
12	3363.0
13	3288.0
14	3093.0
15	3010.0
16	3105.0
17	3309.0
18	3660.0
19	4322.0
20	5483.0
21	7030.0
22	8699.0
23	10381.0
24	11750.0
25	13236.0
26	14527.0
27	15926.0
28	18071.0
29	21585.0
30	27086.0
31	34535.0
32	43920.0
33	57834.0
34	76422.0
35	103519.0
36	148745.0
37	246099.0
38	903085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23732732028959	19.5808710045879	13.637923135228034	27.54387853989448
2	13.798601896808476	20.75939788608821	39.19990051187903	26.242099705224287
3	16.803773656488502	24.997523432543574	29.990599582250493	28.20810332871743
4	11.268185988035754	17.441212843571655	37.31105304451789	33.9795481238747
5	12.681189797845734	38.187524618716395	35.884610131811314	13.246675451626563
6	31.86974019288118	37.66003397567156	17.71523651359291	12.754989317854355
7	27.56283086212313	31.749850841926175	22.457039312376008	18.230278983574692
8	25.27053724863816	36.514031048480376	20.392883058874492	17.82254864400697
9	26.261435077530106	14.682426141294638	19.697551717033438	39.35858706414182
10	14.329562118603834	29.034928393289693	34.7752683769655	21.860241111140972
11	34.338629858660966	22.39778949631372	24.28487423650366	18.97870640852165
12	22.778852679455888	25.004900287061975	31.58366739591369	20.632579637568448
13	28.67663796046891	21.616746968144973	25.828780808578983	23.877834262807138
14	21.040262868921737	21.55358652101244	27.594386846437086	29.81176376362874
15	23.233420168527722	30.056780100408464	23.305870649282767	23.40392908178105
16	22.495057559931038	27.999660668293775	26.909583907668054	22.595697864107137
17	21.91842187784099	28.318238473315432	27.32258379030749	22.440755858536086
18	21.94147401243128	27.48742826031157	28.722755520839755	21.8483422064174
19	23.357191935286703	27.019392490863336	27.47501380510979	22.14840176874017
20	23.588506561115537	27.01001344680923	27.6848303973764	21.71664959469884
21	23.190372187255477	26.533444688289553	27.12849114912519	23.147691975329785
22	23.178159250982823	27.42286390841833	26.970087683569854	22.42888915702899
23	22.488595723468755	27.22988713494327	27.291064748679094	22.990452392908878
24	22.932289624880394	26.966279702062547	27.71186101310537	22.38956965995169
25	22.826931163013224	26.902867777469826	27.37882841916573	22.891372640351218
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	201.0
2	347.0
3	493.0
4	493.0
5	493.0
6	1029.5
7	1566.0
8	1566.0
9	1566.0
10	2356.5
11	3147.0
12	3147.0
13	3147.0
14	5465.5
15	7784.0
16	7784.0
17	7784.0
18	14631.0
19	21478.0
20	21478.0
21	21478.0
22	33584.5
23	45691.0
24	45691.0
25	45691.0
26	65230.0
27	84769.0
28	84769.0
29	84769.0
30	107958.0
31	131147.0
32	131147.0
33	131147.0
34	152201.0
35	173255.0
36	173255.0
37	173255.0
38	190333.0
39	207411.0
40	207411.0
41	207411.0
42	220304.0
43	233197.0
44	233197.0
45	233197.0
46	243493.0
47	253789.0
48	253789.0
49	253789.0
50	246881.0
51	239973.0
52	239973.0
53	239973.0
54	214223.0
55	188473.0
56	188473.0
57	188473.0
58	162162.5
59	135852.0
60	135852.0
61	135852.0
62	111038.0
63	86224.0
64	86224.0
65	86224.0
66	66673.0
67	47122.0
68	47122.0
69	47122.0
70	34643.5
71	22165.0
72	22165.0
73	22165.0
74	15583.5
75	9002.0
76	9002.0
77	9002.0
78	6164.5
79	3327.0
80	3327.0
81	3327.0
82	2304.0
83	1281.0
84	1281.0
85	1281.0
86	836.0
87	391.0
88	391.0
89	391.0
90	246.0
91	101.0
92	101.0
93	101.0
94	54.5
95	8.0
96	8.0
97	8.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17388115381210717
2	0.007060628669946171
3	0.003161475523856494
4	0.0014226639857354223
5	0.07508504369159173
6	0.3111945740649409
7	0.028031749644860913
8	0.01238244580177127
9	0.0036356968524349684
10	0.0026872541952780203
11	0.0
12	0.0
13	0.0
14	0.0010538251746188315
15	0.0
16	0.0
17	0.021234577268569452
18	0.028295205938515623
19	0.0
20	0.0
21	6.849863635022404E-4
22	5.269125873094157E-5
23	0.0050056695794394495
24	0.0
25	1.0538251746188315E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1897848.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.80926019678746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.5129849248579	62.37840894326013
2	10.661236249641723	15.737959207384572
3	2.5095430672941292	5.556825516868695
4	0.9005238116220201	2.6586798530165
5	0.4310108868698976	1.59062973483142
6	0.2544604232895212	1.1268921355416568
7	0.15652888408929014	0.8087296793841441
8	0.10793904764008551	0.6373521002128408
9	0.07679639202349965	0.5101456392933281
>10	0.35812267883915305	4.501890689736145
>50	0.017339291022620835	0.8793788065164029
>100	0.011227129366699225	1.6187395732390017
>500	0.001429508402159224	0.7234886363212375
>1k	8.577050412955314E-4	1.270879484393945
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3887	0.20481092268716988	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2778	0.1463763167545557	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2662	0.14026413074176647	No Hit
CCCATGTACTCTGCGTTGATACCAC	2460	0.12962049647811627	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2342	0.12340292794786516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.5807377619282472E-4	0.0	0.0	0.0	0.0
7	1.5807377619282472E-4	0.0	0.0	0.0	0.0
8	1.5807377619282472E-4	0.0	0.0	0.0	0.0
9	1.5807377619282472E-4	0.0	0.0	5.269125873094157E-5	0.0
10	2.107650349237663E-4	0.0	0.0	5.269125873094157E-5	0.0
11	2.634562936547079E-4	0.0	0.0	5.269125873094157E-5	0.0
12	2.634562936547079E-4	0.0	0.0	5.269125873094157E-5	0.0
13	2.634562936547079E-4	0.0	0.0	5.269125873094157E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGATC	40	0.0052081114	14.27934	3
GGCGAGG	420	0.0	11.748546	19
AGGACGT	1025	0.0	11.329999	5
TAGGACC	480	0.0	11.303879	4
GTATTAA	205	0.0	11.148386	1
GGACGTG	1030	0.0	10.996583	6
TTAGGAC	765	0.0	10.950604	3
TGGCGAG	1085	0.0	10.8473835	18
CAGGACT	360	0.0	10.841147	4
GACGTGA	610	0.0	10.737092	7
TAGGACG	1110	0.0	10.719659	4
TGCTCGA	80	3.8131655E-4	10.6748	10
GTCCTAC	1160	0.0	10.589766	1
AGGACTA	210	0.0	10.425632	5
TCTATAC	165	8.967618E-10	10.3849745	3
AGGACCT	855	0.0	10.354061	5
TCCTACA	1330	0.0	10.236128	2
GATATAG	140	9.3587005E-8	10.202765	1
TGTAGGA	1245	0.0	10.170297	2
CGTGAAA	685	0.0	10.112057	9
>>END_MODULE