FastQCFastQC Report
Thu 26 May 2016
SRR1513161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72302
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC27603.8173217891621256No Hit
CCCATGTACTCTGCGTTGATACCAC26123.6126248236563305No Hit
GAGTACATGGGAAGCAGTGGTATCA12311.7025808414704988No Hit
CATGTACTCTGCGTTGATACCACTG9641.3332964509972063No Hit
GTATCAACGCAGAGTACTTTTTTTT8861.225415617825233No Hit
TATCAACGCAGAGTACATGGGAAGC8191.1327487483057177No Hit
GCGTTGATACCACTGCTTCCCATGT8021.1092362590246465No Hit
ACGCAGAGTACATGGGAAGCAGTGG7271.00550468866698No Hit
GTATCAACGCAGAGTACATGGGAAG7261.0041216010622114No Hit
ACTCTGCGTTGATACCACTGCTTCC6740.9322010456142292No Hit
GGTATCAACGCAGAGTACATGGGAA6560.9073054687283891No Hit
GGTATCAACGCAGAGTACTTTTTTT5670.7842106719039584No Hit
TATCAACGCAGAGTACTTTTTTTTT5170.7150562916655141No Hit
GTGGTATCAACGCAGAGTACATGGG3850.5324887278360211No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3650.5048269757406434No Hit
GTACTCTGCGTTGATACCACTGCTT3300.45641890957373243No Hit
ATACCACTGCTTCCCATGTACTCTG3210.44397112113081244No Hit
GGGAAGCAGTGGTATCAACGCAGAG3010.4163093690354347No Hit
GCTTCCCATGTACTCTGCGTTGATA2950.40801084340682137No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2920.40386158059251476No Hit
GCAGTGGTATCAACGCAGAGTACAT2720.376199828497137No Hit
GATACCACTGCTTCCCATGTACTCT2710.3748167408923681No Hit
CATGGGAAGCAGTGGTATCAACGCA2610.36098586484467926No Hit
CTGCTTCCCATGTACTCTGCGTTGA2490.34438881358745266No Hit
GCAGAGTACATGGGAAGCAGTGGTA2450.3388564631683771No Hit
ACATGGGAAGCAGTGGTATCAACGC2440.3374733755636082No Hit
TACCACTGCTTCCCATGTACTCTGC2440.3374733755636082No Hit
CAGTGGTATCAACGCAGAGTACATG2430.33609028795883933No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2410.33332411274930157No Hit
GTTGATACCACTGCTTCCCATGTAC2380.32917484993499485No Hit
CCACTGCTTCCCATGTACTCTGCGT2220.30704544825869273No Hit
AAGCAGTGGTATCAACGCAGAGTAC2080.2876822217919283No Hit
CCATGTACTCTGCGTTGATACCACT1960.27108517053470166No Hit
CTTCCCATGTACTCTGCGTTGATAC1910.26416973251085724No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1900.26278664490608833No Hit
ATGGGAAGCAGTGGTATCAACGCAG1720.2378910680202484No Hit
CCCATGTACTCTGCGTTTATACCAC1710.23650798041547952No Hit
TTCCCATGTACTCTGCGTTGATACC1620.22406019197255952No Hit
ATCAACGCAGAGTACATGGGAAGCA1510.2088462283201018No Hit
TGATACCACTGCTTCCCATGTACTC1500.20746314071533292No Hit
GTACATGGGAAGCAGTTGTATCAAC1490.20608005311056402No Hit
GAACAAAAAAAAAAAAAAAAAAAAA1470.20331387790102623No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1230.17011977538657297No Hit
GTACATGGGAAGCAGTGTTATCAAC1080.1493734613150397No Hit
GTACATGGTAAGCAGTGGTATCAAC1000.13830876047688861No Hit
AAAAAGTACTCTGCGTTGATACCAC970.13415949766258195No Hit
GTACTGGTTCACTATCGGTCAGTCA950.13139332245304416No Hit
CATTTACTCTGCGTTGATACCACTG790.109263920776742No Hit
GGAAGCAGTGGTATCAACGCAGAGT790.109263920776742No Hit
GAGTACATGGGAATCAGTGGTATCA730.10096539514812869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT250.005759119.1227341
TCACTAT250.00601947118.9771639
ACTATCG250.00601947118.97716311
TTCACTA250.00604361218.9640398
GGTTCAC250.00604361218.9640396
GTTCACT250.00604361218.9640397
ATGGGGA550.00301585612.0931145
CCATGTA6600.08.38487152
CTGCGTT7450.08.02390111
TACTCTG7600.07.98485857
TCTGCGT7500.07.97040810
TGCGTTG7250.07.85261912
CCCATGT6950.07.84169631
CTCTGCG7650.07.81412559
GTACTCT7850.07.7305636
GAAGCAG8400.07.68123259
GTGGTAT7300.07.668853315
TGTACTC7450.07.6524025
TACATGG14000.07.6331252
ACTCTGC7850.07.6097738
Produced by FastQC (version 0.11.5)