##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513161_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72302
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.546596221404663	31.0	26.0	33.0	2.0	34.0
2	26.260407734225886	31.0	26.0	34.0	2.0	34.0
3	26.457068960747975	31.0	26.0	34.0	2.0	34.0
4	29.01485436087522	35.0	27.0	37.0	2.0	37.0
5	29.46025006223894	35.0	30.0	37.0	2.0	37.0
6	29.367597023595476	35.0	30.0	37.0	2.0	37.0
7	29.257973500041494	35.0	30.0	37.0	2.0	37.0
8	28.84243866006473	35.0	28.0	37.0	2.0	37.0
9	29.572335481729414	37.0	27.0	39.0	2.0	39.0
10	29.253519957954136	37.0	25.0	39.0	2.0	39.0
11	29.677394816187658	37.0	27.0	39.0	2.0	39.0
12	29.733963099222706	37.0	27.0	39.0	2.0	39.0
13	29.628972919144697	37.0	27.0	39.0	2.0	39.0
14	29.779245387402838	38.0	25.0	41.0	2.0	41.0
15	30.091228458410555	38.0	27.0	41.0	2.0	41.0
16	29.87236867583193	37.0	27.0	40.0	2.0	41.0
17	29.668736687781806	38.0	25.0	40.0	2.0	41.0
18	29.494163370307874	37.0	25.0	40.0	2.0	41.0
19	30.00394179967359	38.0	27.0	40.0	2.0	41.0
20	30.270352134104176	38.0	27.0	40.0	2.0	41.0
21	30.28570440651711	38.0	28.0	40.0	2.0	41.0
22	30.087812232026778	38.0	27.0	41.0	2.0	41.0
23	29.713742358440985	38.0	27.0	40.0	2.0	41.0
24	29.921869381206605	38.0	27.0	41.0	2.0	41.0
25	29.558366296921246	38.0	27.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.968417318988049
1101	2	2.8456900055082315
1101	3	2.7200424515965835
1101	4	2.4822060029088497
1101	5	2.4989533710565084
1101	6	2.3860573531561293
1101	7	2.2587546602046693
1101	8	2.2946439487096413
1101	9	3.0782198774708434
1101	10	3.0710340778087932
1101	11	2.167455459905529
1101	12	2.5087464888499937
1101	13	1.8559706430886749
1101	14	2.335783011855302
1101	15	2.251183281757161
1101	16	2.4318794175449234
1101	17	3.0408714018126837
1101	18	2.762856676733236
1101	19	2.8128787839506693
1101	20	2.1371733297662665
1101	21	2.317834978667598
1101	22	2.047169275227965
1101	23	2.431676038867142
1101	24	2.5598716206027987
1101	25	2.958640586135715
1102	1	0.30762873170847627
1102	2	0.9829002294036151
1102	3	0.5605417581153098
1102	4	0.259796650818501
1102	5	0.38373641048587004
1102	6	0.4453004678777468
1102	7	-0.07441355853913834
1102	8	-0.5798359403333571
1102	9	0.19492286934720937
1102	10	0.695266294967503
1102	11	0.15527000319857365
1102	12	-0.32660124247554734
1102	13	0.05876438194344047
1102	14	0.5305521816591501
1102	15	0.25207490054059534
1102	16	0.24305437296398225
1102	17	-0.003709537359100068
1102	18	0.15903262283983466
1102	19	0.1245490831383087
1102	20	-0.4469360421378319
1102	21	-0.1930625842410585
1102	22	0.4037177198485331
1102	23	0.531339205104512
1102	24	0.6961902257147443
1102	25	0.6336950739502605
1103	1	1.564505754362198
1103	2	1.8909706177531298
1103	3	1.642661499215631
1103	4	1.348490016514294
1103	5	1.2004839833014032
1103	6	1.5835488497547665
1103	7	0.9600726874155541
1103	8	0.46577490109059383
1103	9	1.5427181767905758
1103	10	1.353134417944851
1103	11	1.439351718408929
1103	12	1.4964165568772039
1103	13	1.7439383301635054
1103	14	2.4584445764811527
1103	15	2.1070506286959336
1103	16	2.18166683251091
1103	17	2.2370873882072466
1103	18	2.5949144998624902
1103	19	1.9270369472159778
1103	20	2.0045202685417784
1103	21	2.155845182749232
1103	22	1.7920672344116397
1103	23	2.6368205966902742
1103	24	1.9367848859089207
1103	25	2.598096368448644
1104	1	0.053420127450884536
1104	2	0.4336885076613868
1104	3	0.20497036771716282
1104	4	0.7629586709485423
1104	5	0.7475023319252578
1104	6	0.3055480852287751
1104	7	0.30191137243084754
1104	8	-0.36000873805313915
1104	9	0.12633820737161372
1104	10	0.37425444852588896
1104	11	0.6577194554119856
1104	12	0.1739777189567171
1104	13	-0.46372604292518105
1104	14	0.5627068088970582
1104	15	0.3851469589711627
1104	16	0.14391836156289628
1104	17	0.1958985502867563
1104	18	0.874539882125486
1104	19	-0.7630433280133815
1104	20	-0.7798834011829925
1104	21	-0.34243463376452965
1104	22	-0.5290336830195521
1104	23	-0.3831526448999405
1104	24	-0.14205685721034556
1104	25	0.32898321860154667
1105	1	0.7240969493307539
1105	2	0.691088542281431
1105	3	0.5569696753162603
1105	4	0.768497878149514
1105	5	0.7405783229240441
1105	6	0.41589947868558497
1105	7	0.4445459578558051
1105	8	0.47658464951826573
1105	9	0.8766411327654176
1105	10	1.1245573739196928
1105	11	-0.053376269012456845
1105	12	0.4402058650533043
1105	13	0.44106883330815805
1105	14	0.2782165890597703
1105	15	0.47143742114876375
1105	16	0.9137816123851223
1105	17	1.1319380171380615
1105	18	0.8052132420008533
1105	19	0.9653624189140899
1105	20	0.7498032874097014
1105	21	0.8857980129379115
1105	22	0.4147087438456012
1105	23	0.9054919803380699
1105	24	1.2793556406259015
1105	25	1.5855177520964396
1106	1	1.58918212976673
1106	2	1.7693079963939056
1106	3	1.036269913455115
1106	4	1.3860102430515084
1106	5	1.8012121386412083
1106	6	1.5909315358389087
1106	7	1.5469456647618323
1106	8	0.8376397878219848
1106	9	1.709485976143327
1106	10	1.5690527027344956
1106	11	1.0873088381500295
1106	12	1.1297094371361034
1106	13	0.9422595275745067
1106	14	2.1227851913678855
1106	15	1.087026356851272
1106	16	0.8352873826727212
1106	17	1.0836691034175985
1106	18	1.9842753412864376
1106	19	1.7070733549829669
1106	20	0.7472387151437196
1106	21	0.3991704254676769
1106	22	0.7143973314990184
1106	23	1.337164447822957
1106	24	1.579685371345814
1106	25	1.255054297251231
1107	1	0.310300861395838
1107	2	0.39699125995496587
1107	3	0.47102541358871974
1107	4	1.5427751847100097
1107	5	1.2429151759619543
1107	6	0.3055480852287751
1107	7	0.41200311555011027
1107	8	0.8785233720386039
1107	9	0.7043198587477626
1107	10	1.4201260081589169
1107	11	0.969646060916574
1107	12	0.6143446914337787
1107	13	1.0867326726711504
1107	14	1.1406884602732035
1107	15	1.0548717296133638
1107	16	0.9971293707372055
1107	17	1.4711279080849202
1107	18	2.1222463041438324
1107	19	1.466314470151758
1107	20	1.1742450391839796
1107	21	1.611693806602446
1107	22	1.8562874178978817
1107	23	1.3599666211551025
1107	24	1.5093192895786451
1107	25	1.668432759885949
1108	1	0.5648680732027778
1108	2	0.8026554003618251
1108	3	0.077806426751863
1108	4	1.0223124802824088
1108	5	1.0124405050405336
1108	6	0.23255247294317272
1108	7	-0.1871193415699537
1108	8	-0.09355480905433566
1108	9	0.23012759708042907
1108	10	0.22586992519122617
1108	11	-0.3183475569533911
1108	12	0.3788440931090875
1108	13	-0.23604355389446852
1108	14	-0.017189481489857883
1108	15	0.6347680200002799
1108	16	0.4725182817862681
1108	17	0.47902579400434675
1108	18	1.402342036094371
1108	19	0.7550260776507614
1108	20	0.5479985294113447
1108	21	0.6716060523144485
1108	22	1.0442411474551179
1108	23	1.4760416111830246
1108	24	1.081078364169791
1108	25	1.7256326003326983
1109	1	1.477667748131669
1109	2	2.326426225229767
1109	3	1.8922720911159452
1109	4	2.1996387704083737
1109	5	2.736698936572431
1109	6	2.584035609879379
1109	7	1.8291342138953048
1109	8	2.014937673676261
1109	9	1.9084697344229724
1109	10	2.6517130783809897
1109	11	1.6491453321160918
1109	12	1.6052561207401332
1109	13	1.7942693270482408
1109	14	2.2003643584126173
1109	15	2.024690815611823
1109	16	2.2224573309532722
1109	17	2.7019433072103602
1109	18	2.2586606057814897
1109	19	1.5809504986178453
1109	20	2.3082585862987877
1109	21	1.481195650038952
1109	22	2.0163662998321996
1109	23	2.467914833450397
1109	24	2.9213059139463056
1109	25	3.0124265865171793
1110	1	0.2699655244771435
1110	2	0.2929102729255426
1110	3	0.5377764417443665
1110	4	1.0902285222614196
1110	5	1.2910012246807128
1110	6	0.7685344819386764
1110	7	0.5179031471856703
1110	8	-0.09205555867952242
1110	9	0.8512670273652887
1110	10	0.9353901650712864
1110	11	0.599193672431916
1110	12	0.7643763269921493
1110	13	0.36283200832442475
1110	14	1.1204417029179368
1110	15	1.6695506286959336
1110	16	1.1144972922810226
1110	17	1.7776764686670141
1110	18	0.8671846148050157
1110	19	0.6436317748021843
1110	20	0.9700375099210881
1110	21	0.9381727689561288
1110	22	0.23172240682543332
1110	23	-0.8462972194016807
1110	24	0.23527626521926592
1110	25	0.4716595868394471
1111	1	0.0037134046354232453
1111	2	0.9345156997203432
1111	3	0.7867871822757415
1111	4	0.8104094154213968
1111	5	1.3746643111702568
1111	6	0.42285895904438675
1111	7	0.8659811573609097
1111	8	0.7360959393419577
1111	9	0.6265638052058762
1111	10	0.5793980791470368
1111	11	0.2311891501029173
1111	12	-0.37482661252170146
1111	13	-0.3320521069949649
1111	14	0.5955688934210457
1111	15	0.30069816967953855
1111	16	-0.18086732049455634
1111	17	1.0733237270050573
1111	18	1.2093270681684984
1111	19	0.6365656357405669
1111	20	0.184848137394237
1111	21	0.2408861663557893
1111	22	0.9641983819526239
1111	23	0.8880159518815276
1111	24	0.5745922629581024
1111	25	1.059845002328423
1112	1	-0.30172231581324027
1112	2	-0.08216534715915103
1112	3	0.03081939395247346
1112	4	0.43566106914587266
1112	5	0.6500453868101737
1112	6	0.6783953409828385
1112	7	0.11303321373134523
1112	8	1.0101827958274363
1112	9	0.9928278259133805
1112	10	1.8021475758395873
1112	11	1.344505227180857
1112	12	0.7847937498596629
1112	13	0.7422935933213992
1112	14	1.7111858045513308
1112	15	1.608147119924002
1112	16	2.599978236019677
1112	17	1.5611005461019793
1112	18	1.4293443244238908
1112	19	1.464865894584399
1112	20	2.267678163278621
1112	21	1.6284329020474786
1112	22	1.7745233747625164
1112	23	2.0671934037078152
1112	24	1.9417190964352358
1112	25	2.4314297017819726
1113	1	-1.3380424550041923
1113	2	-1.336043845056789
1113	3	-0.9706305118036518
1113	4	-1.7605836749457637
1113	5	-0.9472432894258702
1113	6	-1.4695675414298037
1113	7	-1.5945932905458768
1113	8	-1.927906228385993
1113	9	-1.7672852667355947
1113	10	-1.0152367941763636
1113	11	-1.6604244523899716
1113	12	-1.8415655367867068
1113	13	-1.9813543695610143
1113	14	-1.8288315943177444
1113	15	-1.4378873878329905
1113	16	-1.6446933603265563
1113	17	-1.2576612343822866
1113	18	-1.1552576764460518
1113	19	-1.5716717304699728
1113	20	-1.7103557645160734
1113	21	-1.6050019246887857
1113	22	-1.5385112779850552
1113	23	-0.9483205878827299
1113	24	-1.4038659405373615
1113	25	-1.074079940091302
1114	1	-1.9258788610224151
1114	2	-1.3534204078878957
1114	3	-1.6425949110407778
1114	4	-1.432759983485706
1114	5	-3.59759293977552
1114	6	-2.5422524322965145
1114	7	-1.6780149194220506
1114	8	-1.8769067228410314
1114	9	-1.38436515654276
1114	10	-1.4014061803457487
1114	11	-1.9177529824457729
1114	12	-2.113091990131341
1114	13	-2.2451108151356394
1114	14	-7.026331064544486
1114	15	-3.9885690294237257
1114	16	-2.7416771845831107
1114	17	-2.740743808373953
1114	18	-8.658824816376828
1114	19	-3.901755787826758
1114	20	-2.5467617827402727
1114	21	-2.654518065114015
1114	22	-2.6438302014857946
1114	23	-2.69304051442084
1114	24	-2.3140163961423603
1114	25	-2.4531856828334107
1115	1	-0.47169643143014994
1115	2	-0.4179433166730995
1115	3	0.11465603473475738
1115	4	-1.7305741911359789
1115	5	-0.4777947052231859
1115	6	-0.7738623524310242
1115	7	-0.39631392460630366
1115	8	0.07216151311245156
1115	9	0.35607200192718835
1115	10	0.5630046365240844
1115	11	0.16561537961111483
1115	12	-0.6617118584233417
1115	13	-0.4795930906015222
1115	14	-5.888037663956002
1115	15	-2.5599575680253785
1115	16	-0.7382443696748844
1115	17	-0.22995496151953176
1115	18	-0.5365745711757626
1115	19	-0.748680265898777
1115	20	-0.6348239937533044
1115	21	-0.6771466205294558
1115	22	-1.3226868639490164
1115	23	-0.8005086382824054
1115	24	-1.138522491140261
1115	25	-0.8008107353764942
1116	1	-0.302742307653304
1116	2	-0.43630651402981613
1116	3	-0.1858268728773922
1116	4	-0.4482638594546984
1116	5	-0.031105163985419182
1116	6	-0.09741045257081282
1116	7	-0.2882315761503378
1116	8	0.7470249010905938
1116	9	-0.5239000402636904
1116	10	0.28990767375880466
1116	11	-0.2711618474825457
1116	12	-0.20174235785147587
1116	13	-0.5029124450302938
1116	14	0.2348496152408437
1116	15	-0.06688347983119769
1116	16	-0.48427308996971163
1116	17	0.20777537270336666
1116	18	0.3008253525756679
1116	19	0.22088384644078474
1116	20	-0.45672004153574264
1116	21	-0.25161605756084526
1116	22	0.1951680096054389
1116	23	-0.7834797909066289
1116	24	-0.3808023233934037
1116	25	0.29577078705329285
1201	1	0.0010322341129764823
1201	2	-0.12217190561135638
1201	3	-0.06099893692143965
1201	4	0.059451854520524705
1201	5	-0.8147029325864459
1201	6	-0.3598896237654827
1201	7	0.016050101745772594
1201	8	0.08970402881644901
1201	9	-0.04480129361440888
1201	10	0.4461056017454723
1201	11	-1.3134714903138125
1201	12	-0.8433420101009865
1201	13	-0.7290951589330703
1201	14	0.2938223023378512
1201	15	0.3144104417800442
1201	16	0.4747937795514048
1201	17	1.1038124660888649
1201	18	-0.04040481477925795
1201	19	0.19777292852439032
1201	20	-0.16837692771990476
1201	21	-0.266467901362919
1201	22	-0.04905426091546161
1201	23	0.15015782410460332
1201	24	-0.5646753944649099
1201	25	-0.4081341611463749
1202	1	1.4907878056442492
1202	2	1.6433744639069765
1202	3	1.0112512428053755
1202	4	2.4582656575399326
1202	5	1.6331762909937098
1202	6	1.646850131806051
1202	7	1.4993355079283752
1202	8	1.3207428498085427
1202	9	2.032301510123908
1202	10	1.8379100589704898
1202	11	1.2702812055884145
1202	12	1.8409678389284885
1202	13	1.2142908942660675
1202	14	1.8230279098144884
1202	15	2.1310890902343935
1202	16	2.0959296530237275
1202	17	1.997504054873911
1202	18	2.516388858836848
1202	19	1.9294407933698245
1202	20	1.4660587300802383
1202	21	-0.12780866340461472
1202	22	0.9939903113347164
1202	23	1.0558911095107852
1202	24	1.102650270524304
1202	25	1.9089938043460783
1203	1	0.7265427913992362
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2211	14	-2.9323139705273924
2211	15	-3.0654921063468024
2211	16	-7.510907953813877
2211	17	-4.168094235724379
2211	18	-3.2656624232144367
2211	19	-3.3205592066301755
2211	20	-3.914283150261639
2211	21	-3.389560800156751
2211	22	-3.5412660989216924
2211	23	-3.3426131639934873
2211	24	-3.262734344860309
2211	25	-9.80361303326076
2212	1	-2.3076931251896227
2212	2	-5.268955852835106
2212	3	-2.221134018991652
2212	4	-4.6456451375473335
2212	5	-2.2627513108162454
2212	6	-1.971685744082766
2212	7	-1.6566114862539116
2212	8	-1.995272017676914
2212	9	-1.5328245402962608
2212	10	-0.9487738453604706
2212	11	-1.3121363768291658
2212	12	-1.3531982890611687
2212	13	-1.5647976642342556
2212	14	-5.967938476740134
2212	15	-4.985911556178017
2212	16	-2.4276643999820884
2212	17	-6.969932919916005
2212	18	-2.928019673806979
2212	19	-1.8389819603470485
2212	20	-1.8904377146514975
2212	21	-1.5074951533852214
2212	22	-1.4600336059244938
2212	23	-1.3186240811808752
2212	24	-1.9899483073683903
2212	25	-1.7184302421956161
2213	1	-0.9295418646854188
2213	2	-0.983241567120821
2213	3	-0.6160920021849279
2213	4	-0.4607711879674987
2213	5	-0.7249361847658236
2213	6	-0.8120218785365481
2213	7	-0.5809812341530716
2213	8	-0.4322468075928825
2213	9	-0.23870689323743832
2213	10	-4.696673005024337
2213	11	-1.6314641079216052
2213	12	-1.0170638352796573
2213	13	-1.0521926222174471
2213	14	-0.2872662078325696
2213	15	-1.0531384469343195
2213	16	-0.5705215428392343
2213	17	-0.037159810672307
2213	18	-0.12129698473134809
2213	19	-0.822175237657973
2213	20	-0.9744713280968753
2213	21	-0.936066581956652
2213	22	-1.0482689000421423
2213	23	-0.6547585349623901
2213	24	-0.015158391402003701
2213	25	-0.14700167076704318
2214	1	-2.47309587165406
2214	2	-2.7586736911899585
2214	3	-2.6632328097274574
2214	4	-1.9612152680385542
2214	5	-5.373703008568516
2214	6	-3.504103589269622
2214	7	-2.3232098329096473
2214	8	-1.9359019281776995
2214	9	-2.546966782559018
2214	10	-5.250270053599866
2214	11	-3.907955192160177
2214	12	-3.387221654504909
2214	13	-3.368104352763325
2214	14	-2.1251428218928297
2214	15	-2.411750184312197
2214	16	-1.7083230048874682
2214	17	-1.5360325304919442
2214	18	-1.0173822481049903
2214	19	-1.9797211828653225
2214	20	-2.6662114387752958
2214	21	-2.3985247359499553
2214	22	-1.777038456645272
2214	23	-1.8177017610333053
2214	24	-2.06448543929433
2214	25	-2.0848304608196493
2215	1	-2.8815324970039207
2215	2	-6.393353152738673
2215	3	-4.2705898669045865
2215	4	-2.9928692731031923
2215	5	-3.789270115059253
2215	6	-4.347632844234305
2215	7	-3.8635270393604024
2215	8	-4.214723732789189
2215	9	-9.668518162007238
2215	10	-5.9533888060988644
2215	11	-6.006515767929869
2215	12	-5.587941366620061
2215	13	-4.91401932010972
2215	14	-7.3224638934641995
2215	15	-5.5025805188450505
2215	16	-9.582506664756853
2215	17	-5.238151682831006
2215	18	-4.7578860465856
2215	19	-4.72409010196435
2215	20	-4.503676452769696
2215	21	-4.267310554955685
2215	22	-11.519408175424427
2215	23	-11.1693610972988
2215	24	-7.982784786222226
2215	25	-6.636876309146988
2216	1	-0.09772828819099288
2216	2	-0.39160916948091185
2216	3	0.43432816588229883
2216	4	-4.886660692705565
2216	5	-1.5326720450673896
2216	6	-0.5084281005998434
2216	7	-0.2059290856340894
2216	8	-5.9763793542285555
2216	9	-1.597131113989569
2216	10	-0.18609430545940597
2216	11	-0.025408919888946002
2216	12	0.35590237248004186
2216	13	0.9711103159791072
2216	14	0.23880805165845942
2216	15	0.8610399903980621
2216	16	1.1264806622981425
2216	17	1.4700217853703634
2216	18	-1.654642237726165
2216	19	-0.03905347831593531
2216	20	-0.14086980238020885
2216	21	0.48696220402582924
2216	22	0.08284737625561078
2216	23	0.8223259158392082
2216	24	-0.17380908572228648
2216	25	0.9385219003635363
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8490.0
3	1.0
4	50.0
5	369.0
6	723.0
7	573.0
8	498.0
9	347.0
10	341.0
11	311.0
12	373.0
13	315.0
14	273.0
15	255.0
16	200.0
17	238.0
18	253.0
19	378.0
20	534.0
21	673.0
22	822.0
23	912.0
24	958.0
25	953.0
26	977.0
27	946.0
28	1102.0
29	1179.0
30	1344.0
31	1536.0
32	1965.0
33	2517.0
34	3180.0
35	4175.0
36	5996.0
37	8800.0
38	19745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.95704467353952	17.542401064183572	10.704190222813436	21.796364039463473
2	14.02448323189752	23.831639655626567	35.64486836450347	26.49900874797244
3	22.432822162446964	31.615873103907266	25.98513629683037	19.966168436815394
4	12.873144699538608	19.474401022847296	30.472788926566235	37.179665351047866
5	11.44188239209107	42.287267231458245	31.971270503736775	14.299579872713911
6	31.903062494812843	31.333167344454587	18.424765540708773	18.33900462002379
7	24.948818679798595	35.39810767443147	20.605322857300944	19.04775078846899
8	30.576534705508063	30.99980634630813	20.729244473953578	17.69441447423023
9	30.865632002655826	14.886435942125212	17.77187595097795	36.47605610424102
10	18.408421285601648	31.38616463558021	28.19221778042134	22.013196298396803
11	29.936508375638027	27.88513410703664	16.445575643561618	25.73278187376371
12	26.12917952052236	32.49270269896385	25.075048072267492	16.303069708246294
13	31.554338202744574	22.6994798583444	21.110004426737493	24.63617751217353
14	23.04946879150066	28.649291722000886	24.378873395307657	23.922366091190792
15	34.13442913276883	23.930488682273506	21.430350323758923	20.50473186119874
16	18.866802229876473	29.49606451702148	30.082582894136202	21.554550358965844
17	24.34313386371498	30.541681079211347	25.78623313732273	19.32895191975095
18	24.27197897212423	25.14767932489452	29.690807221415234	20.889534481566024
19	22.359174413457282	31.58062195661797	30.093514829570605	15.96668880035414
20	25.139726633833213	26.65320126169	27.086215483371145	21.120856621105638
21	21.56493829893199	28.89436113109402	29.9623706491063	19.578329920867688
22	22.629787469559442	28.670854549479742	22.65469338056232	26.04466460039849
23	18.852549735757172	29.329570294125784	28.41232948728591	23.405550482831135
24	21.48441283502373	34.43013103457819	24.134161696946215	19.951294433451867
25	21.671878026508757	26.21267882343175	23.31829879078004	28.797144359279446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	240.0
2	233.0
3	226.0
4	226.0
5	226.0
6	494.5
7	763.0
8	763.0
9	763.0
10	715.0
11	667.0
12	667.0
13	667.0
14	578.0
15	489.0
16	489.0
17	489.0
18	659.0
19	829.0
20	829.0
21	829.0
22	1112.0
23	1395.0
24	1395.0
25	1395.0
26	1851.5
27	2308.0
28	2308.0
29	2308.0
30	2746.0
31	3184.0
32	3184.0
33	3184.0
34	3422.5
35	3661.0
36	3661.0
37	3661.0
38	4002.0
39	4343.0
40	4343.0
41	4343.0
42	5491.5
43	6640.0
44	6640.0
45	6640.0
46	12236.5
47	17833.0
48	17833.0
49	17833.0
50	15598.5
51	13364.0
52	13364.0
53	13364.0
54	10000.0
55	6636.0
56	6636.0
57	6636.0
58	5093.0
59	3550.0
60	3550.0
61	3550.0
62	3101.5
63	2653.0
64	2653.0
65	2653.0
66	2191.0
67	1729.0
68	1729.0
69	1729.0
70	1337.5
71	946.0
72	946.0
73	946.0
74	735.0
75	524.0
76	524.0
77	524.0
78	376.5
79	229.0
80	229.0
81	229.0
82	148.5
83	68.0
84	68.0
85	68.0
86	41.5
87	15.0
88	15.0
89	15.0
90	11.5
91	8.0
92	8.0
93	8.0
94	5.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18533373903903075
2	0.23650798041547952
3	0.24895576885839948
4	0.4785483112500346
5	0.2503388564631684
6	0.011064700838151089
7	0.013830876047688862
8	0.011064700838151089
9	0.011064700838151089
10	0.012447788442919975
11	0.012447788442919975
12	0.02074631407153329
13	0.019363226466764406
14	0.019363226466764406
15	0.03596027772399104
16	0.015213963652457746
17	0.037343365328759925
18	0.023512489281071063
19	0.019363226466764406
20	0.02489557688583995
21	0.02489557688583995
22	0.04149262814306658
23	0.027661752095377724
24	0.04287571574783547
25	0.03319410251445327
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	72302.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.8090785870377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.40489601964815	45.28367126773809
2	7.408099522144211	7.676136206467318
3	2.1863904535625616	3.398246244917153
4	0.9183373822045436	1.9031285441619872
5	0.5072212285432073	1.3139332245304418
6	0.29365439547238315	0.9128378191474649
7	0.21089724765743878	0.764847445437194
8	0.12814009984249444	0.5311056402312523
9	0.11212258736218265	0.522807114602639
>10	0.6593875971061695	6.654034466543111
>50	0.05339170826770603	1.8865314929047607
>100	0.08275714781494434	9.87109623523554
>500	0.026695854133853014	10.149096843794087
>1k	0.008008756240155903	9.132527454288955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2760	3.8173217891621256	No Hit
CCCATGTACTCTGCGTTGATACCAC	2612	3.6126248236563305	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1231	1.7025808414704988	No Hit
CATGTACTCTGCGTTGATACCACTG	964	1.3332964509972063	No Hit
GTATCAACGCAGAGTACTTTTTTTT	886	1.225415617825233	No Hit
TATCAACGCAGAGTACATGGGAAGC	819	1.1327487483057177	No Hit
GCGTTGATACCACTGCTTCCCATGT	802	1.1092362590246465	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	727	1.00550468866698	No Hit
GTATCAACGCAGAGTACATGGGAAG	726	1.0041216010622114	No Hit
ACTCTGCGTTGATACCACTGCTTCC	674	0.9322010456142292	No Hit
GGTATCAACGCAGAGTACATGGGAA	656	0.9073054687283891	No Hit
GGTATCAACGCAGAGTACTTTTTTT	567	0.7842106719039584	No Hit
TATCAACGCAGAGTACTTTTTTTTT	517	0.7150562916655141	No Hit
GTGGTATCAACGCAGAGTACATGGG	385	0.5324887278360211	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	365	0.5048269757406434	No Hit
GTACTCTGCGTTGATACCACTGCTT	330	0.45641890957373243	No Hit
ATACCACTGCTTCCCATGTACTCTG	321	0.44397112113081244	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	301	0.4163093690354347	No Hit
GCTTCCCATGTACTCTGCGTTGATA	295	0.40801084340682137	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	292	0.40386158059251476	No Hit
GCAGTGGTATCAACGCAGAGTACAT	272	0.376199828497137	No Hit
GATACCACTGCTTCCCATGTACTCT	271	0.3748167408923681	No Hit
CATGGGAAGCAGTGGTATCAACGCA	261	0.36098586484467926	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	249	0.34438881358745266	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	245	0.3388564631683771	No Hit
ACATGGGAAGCAGTGGTATCAACGC	244	0.3374733755636082	No Hit
TACCACTGCTTCCCATGTACTCTGC	244	0.3374733755636082	No Hit
CAGTGGTATCAACGCAGAGTACATG	243	0.33609028795883933	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	241	0.33332411274930157	No Hit
GTTGATACCACTGCTTCCCATGTAC	238	0.32917484993499485	No Hit
CCACTGCTTCCCATGTACTCTGCGT	222	0.30704544825869273	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	208	0.2876822217919283	No Hit
CCATGTACTCTGCGTTGATACCACT	196	0.27108517053470166	No Hit
CTTCCCATGTACTCTGCGTTGATAC	191	0.26416973251085724	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	190	0.26278664490608833	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	172	0.2378910680202484	No Hit
CCCATGTACTCTGCGTTTATACCAC	171	0.23650798041547952	No Hit
TTCCCATGTACTCTGCGTTGATACC	162	0.22406019197255952	No Hit
ATCAACGCAGAGTACATGGGAAGCA	151	0.2088462283201018	No Hit
TGATACCACTGCTTCCCATGTACTC	150	0.20746314071533292	No Hit
GTACATGGGAAGCAGTTGTATCAAC	149	0.20608005311056402	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	147	0.20331387790102623	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	123	0.17011977538657297	No Hit
GTACATGGGAAGCAGTGTTATCAAC	108	0.1493734613150397	No Hit
GTACATGGTAAGCAGTGGTATCAAC	100	0.13830876047688861	No Hit
AAAAAGTACTCTGCGTTGATACCAC	97	0.13415949766258195	No Hit
GTACTGGTTCACTATCGGTCAGTCA	95	0.13139332245304416	No Hit
CATTTACTCTGCGTTGATACCACTG	79	0.109263920776742	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	79	0.109263920776742	No Hit
GAGTACATGGGAATCAGTGGTATCA	73	0.10096539514812869	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.001383087604768886	0.0	0.0	0.0	0.0
9	0.001383087604768886	0.0	0.0	0.0	0.0
10	0.001383087604768886	0.0	0.0	0.0	0.0
11	0.001383087604768886	0.0	0.0	0.0	0.0
12	0.001383087604768886	0.0	0.0	0.0	0.0
13	0.001383087604768886	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAGT	25	0.0057591	19.122734	1
TCACTAT	25	0.006019471	18.977163	9
ACTATCG	25	0.006019471	18.977163	11
TTCACTA	25	0.006043612	18.964039	8
GGTTCAC	25	0.006043612	18.964039	6
GTTCACT	25	0.006043612	18.964039	7
ATGGGGA	55	0.003015856	12.093114	5
CCATGTA	660	0.0	8.3848715	2
CTGCGTT	745	0.0	8.023901	11
TACTCTG	760	0.0	7.9848585	7
TCTGCGT	750	0.0	7.970408	10
TGCGTTG	725	0.0	7.852619	12
CCCATGT	695	0.0	7.8416963	1
CTCTGCG	765	0.0	7.8141255	9
GTACTCT	785	0.0	7.730563	6
GAAGCAG	840	0.0	7.6812325	9
GTGGTAT	730	0.0	7.6688533	15
TGTACTC	745	0.0	7.652402	5
TACATGG	1400	0.0	7.633125	2
ACTCTGC	785	0.0	7.609773	8
>>END_MODULE