FastQCFastQC Report
Thu 26 May 2016
SRR1513161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72302
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC32584.506099416337031No Hit
GTACATGGGAAGCAGTGGTATCAAC31434.347044341788609No Hit
GTATCAACGCAGAGTACTTTTTTTT16552.2890099858925064No Hit
GAGTACATGGGAAGCAGTGGTATCA13631.8851484052999918No Hit
CATGTACTCTGCGTTGATACCACTG11961.6541727753035875No Hit
TATCAACGCAGAGTACTTTTTTTTT11441.5822522198556055No Hit
GGTATCAACGCAGAGTACTTTTTTT10081.394152305607037No Hit
TATCAACGCAGAGTACATGGGAAGC9891.3678736411164283No Hit
ACGCAGAGTACATGGGAAGCAGTGG9261.2807391220159885No Hit
CTTATACACATCTCCGAGCCCACGA8731.2074354789632376No Hit
ACTCTGCGTTGATACCACTGCTTCC8431.165942850820171No Hit
GCGTTGATACCACTGCTTCCCATGT8071.116151697048491No Hit
GTATCAACGCAGAGTACATGGGAAG7691.0635943680672733No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6990.9667782357334515No Hit
GGTATCAACGCAGAGTACATGGGAA6510.9003900307045448No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6320.874111366213936No Hit
ATACACATCTCCGAGCCCACGAGAC5100.705374678432132No Hit
TCTCCGAGCCCACGAGACCTCTCTA4770.6597327874747587No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4450.6154739841221544No Hit
ATACCACTGCTTCCCATGTACTCTG4230.5850460568172388No Hit
GTGGTATCAACGCAGAGTACATGGG3880.5366379906503278No Hit
GCTTCCCATGTACTCTGCGTTGATA3810.5269563774169456No Hit
ACACATCTCCGAGCCCACGAGACCT3740.5172747641835634No Hit
GTACTCTGCGTTGATACCACTGCTT3580.4951453625072612No Hit
GATACCACTGCTTCCCATGTACTCT3350.4633343475975768No Hit
GGGAAGCAGTGGTATCAACGCAGAG3310.4578019971785013No Hit
CTGCTTCCCATGTACTCTGCGTTGA3250.449503471549888No Hit
CAGTGGTATCAACGCAGAGTACATG3180.4398218583165058No Hit
TACCACTGCTTCCCATGTACTCTGC3110.43014024508312354No Hit
GCAGAGTACATGGGAAGCAGTGGTA3070.42460789466404797No Hit
ACATGGGAAGCAGTGGTATCAACGC3030.41907554424497245No Hit
CATGGGAAGCAGTGGTATCAACGCA2900.40109540538297694No Hit
GCAGTGGTATCAACGCAGAGTACAT2730.37758291610190586No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2690.37205056568283035No Hit
AAGCAGTGGTATCAACGCAGAGTAC2650.3665182152637548No Hit
GTTGATACCACTGCTTCCCATGTAC2550.352687339216066No Hit
CCACTGCTTCCCATGTACTCTGCGT2470.34162263837791484No Hit
CCATGTACTCTGCGTTGATACCACT2350.32502558712068824No Hit
CTTCCCATGTACTCTGCGTTGATAC2260.31257779867776825No Hit
ATGGGAAGCAGTGGTATCAACGCAG2120.29321457221100383No Hit
TTCCCATGTACTCTGCGTTGATACC1950.26970208292993275No Hit
ATCAACGCAGAGTACATGGGAAGCA1830.25310503167270615No Hit
TGATACCACTGCTTCCCATGTACTC1800.24895576885839948No Hit
ATCAACGCAGAGTACTTTTTTTTTT1700.2351248928107106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1660.22959254239163507No Hit
CATCTCCGAGCCCACGAGACCTCTC1560.21576166634394625No Hit
GTGGTATCAACGCAGAGTACTTTTT1460.20193079029625738No Hit
CTCCGAGCCCACGAGACCTCTCTAC1270.17565212580564854No Hit
AAAAAGTACTCTGCGTTGATACCAC1180.16320433736272855No Hit
GGAAGCAGTGGTATCAACGCAGAGT1010.1396918480816575No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT990.1369256728721197No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT940.13001023484827529No Hit
GTACATGGTAAGCAGTGGTATCAAC920.12724405963873753No Hit
CACATCTCCGAGCCCACGAGACCTC910.12586097203396862No Hit
ACCATGTACTCTGCGTTGATACCAC860.1189455340101242No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT830.11479627119581753No Hit
AAAGTACTCTGCGTTGATACCACTG820.11341318359104864No Hit
GTACTGGTTCACTATCGGTCAGTCA800.1106470083815109No Hit
TCAACGCAGAGTACATGGGAAGCAG740.10234848275289757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA1350.016.9500852
CTTATAC1400.016.3447251
TATACAC1450.015.7701463
GCAGAGA500.001443313413.3389163
TACACAT1850.012.3603865
ATACACA1950.011.726524
CCTCTCT657.9444086E-411.67782818
CTCTCTA657.990776E-411.66975219
ACACATC2400.09.5277986
CACATCT2401.8189894E-129.4882357
CCATGTA7100.09.2658472
ACATCTC2451.4551915E-118.9011638
AGTGGTA8050.08.604238514
AGCAGTG8200.08.56255311
GAAGCAG7600.08.4836039
CCCATGT7200.08.4750431
AAGCAGT8300.08.45354110
CAGTGGT8200.08.44684313
TACTCTG7650.08.4339877
ATCTCCG2605.4569682E-118.38763410
Produced by FastQC (version 0.11.5)