##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513161_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72302 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.75350612707809 31.0 30.0 34.0 20.0 34.0 2 28.706301347127326 31.0 29.0 34.0 16.0 34.0 3 28.833835855163066 31.0 29.0 34.0 20.0 34.0 4 31.981826228873338 35.0 32.0 37.0 19.0 37.0 5 31.74769715913806 35.0 32.0 37.0 17.0 37.0 6 31.38867527869215 36.0 32.0 37.0 8.0 37.0 7 31.06689994744267 36.0 32.0 37.0 8.0 37.0 8 30.96315454620896 36.0 32.0 37.0 7.0 37.0 9 32.18874996542281 39.0 32.0 39.0 2.0 39.0 10 32.12530773699206 38.0 32.0 39.0 2.0 39.0 11 32.08673342369506 38.0 32.0 39.0 2.0 39.0 12 32.00351304251611 38.0 32.0 39.0 2.0 39.0 13 31.8788276949462 38.0 32.0 39.0 2.0 39.0 14 32.78123703355371 39.0 32.0 41.0 2.0 41.0 15 32.70045088655915 39.0 32.0 41.0 2.0 41.0 16 32.512779729468065 38.0 32.0 41.0 2.0 41.0 17 32.664407623578875 39.0 32.0 41.0 2.0 41.0 18 32.66205637465077 39.0 32.0 41.0 2.0 41.0 19 32.67005062100633 39.0 32.0 41.0 2.0 41.0 20 32.777475035268736 39.0 32.0 41.0 2.0 41.0 21 32.696785704406516 39.0 32.0 41.0 2.0 41.0 22 32.717974606511575 39.0 32.0 41.0 2.0 41.0 23 32.6325689469171 39.0 32.0 41.0 2.0 41.0 24 32.50894857680286 39.0 32.0 41.0 2.0 41.0 25 32.49510386987912 39.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.9323463108092227 1101 2 2.2005689855277133 1101 3 1.8879824877944316 1101 4 1.8601713379238092 1101 5 2.062378345817457 1101 6 1.4171050063169481 1101 7 2.6164537123616114 1101 8 2.16021318525339 1101 9 2.4880191705824046 1101 10 3.311087310003984 1101 11 3.4195412316117313 1101 12 3.492143029332084 1101 13 2.888714216983182 1101 14 2.967681027420298 1101 15 3.3689820126686527 1101 16 3.281906288924631 1101 17 3.0591493908257235 1101 18 3.804016421220986 1101 19 3.4887286848658263 1101 20 3.470455166363564 1101 21 3.357433360550793 1101 22 3.383489750169538 1101 23 3.662311432043552 1101 24 3.8416695805620975 1101 25 3.6055631895721376 1102 1 -0.2262627638886201 1102 2 -0.09168383793176815 1102 3 -0.08665591125450689 1102 4 0.07673563354497048 1102 5 -0.4589214362312859 1102 6 -1.0832912686979128 1102 7 -0.8353988733328599 1102 8 -0.7513184176790446 1102 9 -1.3098805718388356 1102 10 -0.8413795019635195 1102 11 -0.43690814425512414 1102 12 -0.6065294493681321 1102 13 0.503336765031527 1102 14 0.07744920653660614 1102 15 0.48330735445585304 1102 16 -0.6389377768570199 1102 17 0.01456845165394327 1102 18 -0.6267345199321781 1102 19 -0.24953166781893543 1102 20 0.2515132206532442 1102 21 -0.3365432592233262 1102 22 0.20060883081150394 1102 23 -0.422194214875411 1102 24 -0.03023452088430645 1102 25 -0.04383249103020148 1103 1 1.3462493720337108 1103 2 1.128077488929076 1103 3 1.1145981340529367 1103 4 1.4943124943863921 1103 5 0.8840195022800401 1103 6 1.3735250743441618 1103 7 0.9062071137221537 1103 8 1.2305788315118917 1103 9 1.1929511433715163 1103 10 0.6556876501400382 1103 11 0.12043408875458539 1103 12 0.4324066347742601 1103 13 0.6895220401124362 1103 14 0.4039055172162165 1103 15 -0.07383261318168621 1103 16 0.9791852004892547 1103 17 1.3595320438869507 1103 18 0.4951303667992164 1103 19 0.9972320862263686 1103 20 1.5852510847309134 1103 21 1.446931659870522 1103 22 0.7661428113940261 1103 23 1.1319117721796061 1103 24 1.2179450907661789 1103 25 1.0613455024973106 1104 1 1.3494030417584817 1104 2 1.11594063877617 1104 3 1.6709819261018666 1104 4 2.1415793139582604 1104 5 1.7566303585491525 1104 6 1.6557307318365169 1104 7 1.5523271443031987 1104 8 1.7276162932855925 1104 9 1.642110164778245 1104 10 1.5684361210880553 1104 11 1.5565006025160528 1104 12 1.0796734543461284 1104 13 1.5187460462286424 1104 14 1.9463367098767677 1104 15 1.9497721268794734 1104 16 1.6519680751069927 1104 17 1.2141765392997925 1104 18 1.0296296022732179 1104 19 1.5576296397431904 1104 20 1.2171318186758668 1104 21 1.410138846414867 1104 22 1.006968499467419 1104 23 0.9196602431276233 1104 24 0.7850322467294788 1104 25 1.145443361824526 1105 1 0.4329239003355987 1105 2 0.6895947845265589 1105 3 0.8311861843674002 1105 4 1.3081882805499134 1105 5 0.5953009488209204 1105 6 0.5089417410108261 1105 7 0.8026300696969955 1105 8 0.5468131082414551 1105 9 0.7644920238746664 1105 10 1.3363087193224317 1105 11 1.3615897491319444 1105 12 1.5576031756547621 1105 13 1.3555990841375944 1105 14 1.236452687027537 1105 15 1.359933110088754 1105 16 1.2276128734452314 1105 17 -0.05733116366021562 1105 18 0.3569621278055024 1105 19 1.3541503252200826 1105 20 0.9602510847309134 1105 21 1.3917036724491396 1105 22 0.9689730000732695 1105 23 1.1214951055129418 1105 24 0.9313884869925957 1105 25 1.322351791805481 1106 1 -0.158301598840076 1106 2 0.015112278573088389 1106 3 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-0.1603196162397893 1107 16 5.919283180020329E-4 1107 17 0.1683049796667646 1107 18 -0.2822970032313705 1107 19 0.016345236073462388 1107 20 0.7584162223455948 1107 21 0.30004710329560424 1107 22 0.7225648297426517 1107 23 0.3325042798248674 1107 24 0.4088854577386556 1107 25 0.686727765494247 1108 1 0.4215211111641466 1108 2 0.8062477787841473 1108 3 0.06260538042974773 1108 4 0.7648740885892948 1108 5 0.9322984877872855 1108 6 1.0045939149238698 1108 7 0.8648121861859224 1108 8 1.026140425714793 1108 9 1.1904149114874585 1108 10 1.022444896516852 1108 11 1.020162349624151 1108 12 0.9725334463684661 1108 13 0.8368952285182303 1108 14 1.4984707346075226 1108 15 1.2893014447893236 1108 16 0.7212141859965016 1108 17 0.6625211743217321 1108 18 1.4609818160745789 1108 19 1.3153480282553573 1108 20 0.9776423890787385 1108 21 1.5310802105951637 1108 22 1.2944036809592419 1108 23 0.9562777142085963 1108 24 1.393488569027049 1108 25 1.1323599952509298 1109 1 0.6631302131552061 1109 2 1.0212823175895096 1109 3 0.5992165141152412 1109 4 0.5101808744487002 1109 5 0.5067804057068344 1109 6 1.0883809933472754 1109 7 1.5400661479900677 1109 8 1.5022883330695294 1109 9 1.3148358785740086 1109 10 1.7235420115107587 1109 11 1.0570579205302835 1109 12 1.5043497811917064 1109 13 1.6882564637884485 1109 14 2.421428881702198 1109 15 2.058663492737317 1109 16 2.452705449710443 1109 17 1.8230250345411534 1109 18 2.0370890895406504 1109 19 1.7908457933914832 1109 20 2.137821178188858 1109 21 1.9960944324561964 1109 22 1.9133390730762656 1109 23 1.626168002709207 1109 24 1.6075478945044992 1109 25 1.3775448794443506 1110 1 0.09948213065440115 1110 2 0.06809185674516627 1110 3 0.3380176742828205 1110 4 0.3696716897886958 1110 5 0.8218786976823438 1110 6 0.5865279479073813 1110 7 0.19104906774514419 1110 8 0.24817940622453705 1110 9 0.4357672353255424 1110 10 0.14958132830096105 1110 11 0.19909788185803023 1110 12 0.3939727267282862 1110 13 0.4240766378135845 1110 14 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2216 13 -0.3408414350648705 2216 14 -0.7370519295922975 2216 15 -0.6347811947419757 2216 16 -0.23358075695755076 2216 17 -0.41240944547475067 2216 18 -0.060942328236244236 2216 19 0.35006896565899837 2216 20 -0.3163446599499409 2216 21 -0.3356481273635197 2216 22 0.2927385560748803 2216 23 0.2278780842363446 2216 24 -0.598987533347298 2216 25 -0.4741154904104903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4782.0 3 0.0 4 55.0 5 542.0 6 930.0 7 572.0 8 427.0 9 308.0 10 295.0 11 259.0 12 222.0 13 188.0 14 192.0 15 163.0 16 144.0 17 165.0 18 179.0 19 306.0 20 399.0 21 482.0 22 544.0 23 664.0 24 654.0 25 654.0 26 680.0 27 794.0 28 780.0 29 973.0 30 1119.0 31 1407.0 32 1840.0 33 2315.0 34 2948.0 35 4058.0 36 5715.0 37 9776.0 38 26771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39983374896093 19.14103629814353 12.642006095871434 24.817123857024107 2 11.617838271502476 22.93827980191994 35.69867485545135 29.745207071126234 3 19.206030845839962 30.588560758005396 28.613320423265787 21.59208797288886 4 11.303060899874135 19.99197775902848 27.880053666025812 40.82490767507158 5 10.3479777427124 40.47559726490048 33.615701907372035 15.560723085015088 6 31.967975134940126 31.596109284158235 18.047981795224022 18.387933785677614 7 22.277405788279562 33.75297437883902 21.683913452492945 22.28570638038847 8 30.738691381933876 30.1521648914096 21.11495365887398 17.994190067782544 9 27.796680497925312 12.96957123098202 18.39557399723375 40.838174273858925 10 17.48409405255878 31.03596127247579 28.98340248962656 22.496542185338868 11 28.372659124228928 25.374125197089985 18.433791596359715 27.819424082321376 12 24.361705070399157 31.130535808138088 26.440485740366793 18.06727338109596 13 32.66852922464109 19.248430195568584 23.435036375204003 24.648004204586318 14 23.003513042516115 26.187380708694093 25.076761362064676 25.732344886725123 15 33.81095958618019 21.70479378163813 22.6217808635999 21.86246576858178 16 19.855605654062128 25.733727974329895 31.429282730768165 22.981383640839812 17 23.097780975042888 29.75485584638371 27.434840351945105 19.712522826628298 18 25.508453471319076 20.695093942831843 31.53241650294695 22.264036082902127 19 21.56648502116124 28.01858869740809 33.37113772786368 17.043788553566984 20 23.150120328621615 22.53464634449946 31.25086442975298 23.064368897125945 21 20.389477469502918 25.150065005117423 32.997704074576085 21.462753450803575 22 20.318940001659705 26.74061575060164 26.95499432934082 25.98544991839783 23 17.405496618210485 24.70850219228482 31.263226324015548 26.622774865489152 24 21.15570800254488 31.15266520981439 28.566291388896577 19.125335398744156 25 20.27606428591187 23.022876268982877 26.64518270587259 30.055876739232662 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 280.0 1 280.0 2 295.5 3 311.0 4 311.0 5 311.0 6 572.5 7 834.0 8 834.0 9 834.0 10 713.0 11 592.0 12 592.0 13 592.0 14 424.5 15 257.0 16 257.0 17 257.0 18 509.5 19 762.0 20 762.0 21 762.0 22 1157.5 23 1553.0 24 1553.0 25 1553.0 26 2140.5 27 2728.0 28 2728.0 29 2728.0 30 3084.0 31 3440.0 32 3440.0 33 3440.0 34 3414.5 35 3389.0 36 3389.0 37 3389.0 38 3451.5 39 3514.0 40 3514.0 41 3514.0 42 4082.5 43 4651.0 44 4651.0 45 4651.0 46 11270.5 47 17890.0 48 17890.0 49 17890.0 50 16167.0 51 14444.0 52 14444.0 53 14444.0 54 10663.5 55 6883.0 56 6883.0 57 6883.0 58 5615.0 59 4347.0 60 4347.0 61 4347.0 62 3703.0 63 3059.0 64 3059.0 65 3059.0 66 2367.0 67 1675.0 68 1675.0 69 1675.0 70 1293.0 71 911.0 72 911.0 73 911.0 74 701.5 75 492.0 76 492.0 77 492.0 78 352.5 79 213.0 80 213.0 81 213.0 82 140.0 83 67.0 84 67.0 85 67.0 86 37.5 87 8.0 88 8.0 89 8.0 90 5.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1687366877818041 2 0.011064700838151089 3 0.009681613233382203 4 0.0041492628143066585 5 0.07745290586705762 6 0.3222594119111505 7 0.02489557688583995 8 0.016597051257226634 9 0.002766175209537772 10 0.002766175209537772 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.02489557688583995 18 0.03319410251445327 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0041492628143066585 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 72302.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.329423805703854 #Duplication Level Percentage of deduplicated Percentage of total 1 84.30493273542601 34.84274293933778 2 9.25640854025835 7.651240629581478 3 2.767552372665819 3.4314403474316064 4 1.2214711197376347 2.0193079029625736 5 0.6559132588180175 1.3554258526735083 6 0.33464962184592734 0.8298525628613317 7 0.24764072016598623 0.7164393792702829 8 0.15059232983066728 0.49791153771679897 9 0.12716685630145239 0.47301596083095904 >10 0.6927247172210695 5.42585267350834 >50 0.073622916806104 2.154850488229924 >100 0.11043437520915601 12.295648806395397 >500 0.03346496218459273 10.648391469115653 >1k 0.02342547352921491 17.65787945008437 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 3258 4.506099416337031 No Hit GTACATGGGAAGCAGTGGTATCAAC 3143 4.347044341788609 No Hit GTATCAACGCAGAGTACTTTTTTTT 1655 2.2890099858925064 No Hit GAGTACATGGGAAGCAGTGGTATCA 1363 1.8851484052999918 No Hit CATGTACTCTGCGTTGATACCACTG 1196 1.6541727753035875 No Hit TATCAACGCAGAGTACTTTTTTTTT 1144 1.5822522198556055 No Hit GGTATCAACGCAGAGTACTTTTTTT 1008 1.394152305607037 No Hit TATCAACGCAGAGTACATGGGAAGC 989 1.3678736411164283 No Hit ACGCAGAGTACATGGGAAGCAGTGG 926 1.2807391220159885 No Hit CTTATACACATCTCCGAGCCCACGA 873 1.2074354789632376 No Hit ACTCTGCGTTGATACCACTGCTTCC 843 1.165942850820171 No Hit GCGTTGATACCACTGCTTCCCATGT 807 1.116151697048491 No Hit GTATCAACGCAGAGTACATGGGAAG 769 1.0635943680672733 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 699 0.9667782357334515 No Hit GGTATCAACGCAGAGTACATGGGAA 651 0.9003900307045448 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 632 0.874111366213936 No Hit ATACACATCTCCGAGCCCACGAGAC 510 0.705374678432132 No Hit TCTCCGAGCCCACGAGACCTCTCTA 477 0.6597327874747587 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 445 0.6154739841221544 No Hit ATACCACTGCTTCCCATGTACTCTG 423 0.5850460568172388 No Hit GTGGTATCAACGCAGAGTACATGGG 388 0.5366379906503278 No Hit GCTTCCCATGTACTCTGCGTTGATA 381 0.5269563774169456 No Hit ACACATCTCCGAGCCCACGAGACCT 374 0.5172747641835634 No Hit GTACTCTGCGTTGATACCACTGCTT 358 0.4951453625072612 No Hit GATACCACTGCTTCCCATGTACTCT 335 0.4633343475975768 No Hit GGGAAGCAGTGGTATCAACGCAGAG 331 0.4578019971785013 No Hit CTGCTTCCCATGTACTCTGCGTTGA 325 0.449503471549888 No Hit CAGTGGTATCAACGCAGAGTACATG 318 0.4398218583165058 No Hit TACCACTGCTTCCCATGTACTCTGC 311 0.43014024508312354 No Hit GCAGAGTACATGGGAAGCAGTGGTA 307 0.42460789466404797 No Hit ACATGGGAAGCAGTGGTATCAACGC 303 0.41907554424497245 No Hit CATGGGAAGCAGTGGTATCAACGCA 290 0.40109540538297694 No Hit GCAGTGGTATCAACGCAGAGTACAT 273 0.37758291610190586 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 269 0.37205056568283035 No Hit AAGCAGTGGTATCAACGCAGAGTAC 265 0.3665182152637548 No Hit GTTGATACCACTGCTTCCCATGTAC 255 0.352687339216066 No Hit CCACTGCTTCCCATGTACTCTGCGT 247 0.34162263837791484 No Hit CCATGTACTCTGCGTTGATACCACT 235 0.32502558712068824 No Hit CTTCCCATGTACTCTGCGTTGATAC 226 0.31257779867776825 No Hit ATGGGAAGCAGTGGTATCAACGCAG 212 0.29321457221100383 No Hit TTCCCATGTACTCTGCGTTGATACC 195 0.26970208292993275 No Hit ATCAACGCAGAGTACATGGGAAGCA 183 0.25310503167270615 No Hit TGATACCACTGCTTCCCATGTACTC 180 0.24895576885839948 No Hit ATCAACGCAGAGTACTTTTTTTTTT 170 0.2351248928107106 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 166 0.22959254239163507 No Hit CATCTCCGAGCCCACGAGACCTCTC 156 0.21576166634394625 No Hit GTGGTATCAACGCAGAGTACTTTTT 146 0.20193079029625738 No Hit CTCCGAGCCCACGAGACCTCTCTAC 127 0.17565212580564854 No Hit AAAAAGTACTCTGCGTTGATACCAC 118 0.16320433736272855 No Hit GGAAGCAGTGGTATCAACGCAGAGT 101 0.1396918480816575 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 99 0.1369256728721197 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 94 0.13001023484827529 No Hit GTACATGGTAAGCAGTGGTATCAAC 92 0.12724405963873753 No Hit CACATCTCCGAGCCCACGAGACCTC 91 0.12586097203396862 No Hit ACCATGTACTCTGCGTTGATACCAC 86 0.1189455340101242 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 83 0.11479627119581753 No Hit AAAGTACTCTGCGTTGATACCACTG 82 0.11341318359104864 No Hit GTACTGGTTCACTATCGGTCAGTCA 80 0.1106470083815109 No Hit TCAACGCAGAGTACATGGGAAGCAG 74 0.10234848275289757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.001383087604768886 8 0.0 0.0 0.0 0.0 0.001383087604768886 9 0.0 0.0 0.0 0.0 0.001383087604768886 10 0.0 0.0 0.0 0.0 0.001383087604768886 11 0.0 0.0 0.0 0.0 0.001383087604768886 12 0.0 0.0 0.0 0.0 0.001383087604768886 13 0.0 0.0 0.0 0.0 0.001383087604768886 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACA 135 0.0 16.950085 2 CTTATAC 140 0.0 16.344725 1 TATACAC 145 0.0 15.770146 3 GCAGAGA 50 0.0014433134 13.338916 3 TACACAT 185 0.0 12.360386 5 ATACACA 195 0.0 11.72652 4 CCTCTCT 65 7.9444086E-4 11.677828 18 CTCTCTA 65 7.990776E-4 11.669752 19 ACACATC 240 0.0 9.527798 6 CACATCT 240 1.8189894E-12 9.488235 7 CCATGTA 710 0.0 9.265847 2 ACATCTC 245 1.4551915E-11 8.901163 8 AGTGGTA 805 0.0 8.6042385 14 AGCAGTG 820 0.0 8.562553 11 GAAGCAG 760 0.0 8.483603 9 CCCATGT 720 0.0 8.475043 1 AAGCAGT 830 0.0 8.453541 10 CAGTGGT 820 0.0 8.446843 13 TACTCTG 765 0.0 8.433987 7 ATCTCCG 260 5.4569682E-11 8.387634 10 >>END_MODULE