FastQCFastQC Report
Thu 26 May 2016
SRR1513160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118198
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22640.10688330363828123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4350.012.0486774
GGTCGGT752.0962159E-411.38721811
CTAGGAC3100.011.373813
GTAGGAC7900.011.21816353
GACACGC700.001507594810.8434337
TGTAGGA9050.010.4318582
GTCTTAC1951.2732926E-1110.2858581
CTGTAGG9500.010.2549231
TAGGACG5350.010.1528274
GGTATCA12250.010.1359191
GTCTTAG2750.010.0721251
GTATCAA23000.010.0494761
TGGTCGG951.663576E-49.98855210
TAGGACA4150.09.8738444
GTATTGG2650.09.7313651
CCTACAC3150.09.6806693
AGGACGT5250.09.6014935
GTTCTAG2201.4551915E-119.5511541
GTACTAA900.0010604629.5511541
AAACGAA900.00112559369.48912415
Produced by FastQC (version 0.11.5)