##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2118198 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.26046195870263 31.0 30.0 34.0 23.0 34.0 2 29.081261525126546 31.0 30.0 34.0 16.0 34.0 3 29.258423433503385 31.0 30.0 34.0 22.0 34.0 4 32.30442102201966 35.0 33.0 37.0 19.0 37.0 5 32.44376210344831 37.0 33.0 37.0 22.0 37.0 6 32.806943449101546 37.0 35.0 37.0 25.0 37.0 7 32.7515784643362 37.0 35.0 37.0 25.0 37.0 8 32.63598681520802 37.0 35.0 37.0 25.0 37.0 9 33.85357270661194 39.0 34.0 39.0 22.0 39.0 10 33.41454339962553 38.0 33.0 39.0 17.0 39.0 11 33.885736366477545 39.0 34.0 39.0 23.0 39.0 12 33.87641712436703 39.0 34.0 39.0 23.0 39.0 13 33.920612709482306 39.0 35.0 39.0 23.0 39.0 14 34.13061432406224 39.0 33.0 41.0 11.0 41.0 15 34.561241678067866 39.0 34.0 41.0 17.0 41.0 16 34.40388811621954 39.0 34.0 41.0 17.0 41.0 17 34.01319187346981 39.0 33.0 41.0 11.0 41.0 18 33.7406994058157 39.0 33.0 41.0 10.0 41.0 19 34.378872985433844 39.0 34.0 41.0 18.0 41.0 20 34.58522196697381 39.0 34.0 41.0 18.0 41.0 21 34.63678702368711 40.0 34.0 41.0 18.0 41.0 22 34.32027459189368 40.0 34.0 41.0 10.0 41.0 23 33.856134790043235 39.0 34.0 41.0 8.0 41.0 24 34.102069306079976 39.0 34.0 41.0 2.0 41.0 25 33.74693631095865 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8428878827573101 1101 2 1.124934688378275 1101 3 1.0216151684283403 1101 4 1.3693084218309295 1101 5 1.2111954064540384 1101 6 1.007750037246602 1101 7 1.0482520045367494 1101 8 1.079226374069087 1101 9 1.3572711310566845 1101 10 1.7708041295956392 1101 11 1.3391152164923597 1101 12 1.2375983872783678 1101 13 1.1281040623985348 1101 14 2.1655135864865542 1101 15 1.7770917090197926 1101 16 1.8394486876312328 1101 17 2.2009736933209396 1101 18 2.191577976942739 1101 19 1.7410401442419285 1101 20 1.4197840363287213 1101 21 1.4577886218564657 1101 22 1.6701303173435278 1101 23 1.9305632583170365 1101 24 1.6160483200939453 1101 25 1.811731465798836 1102 1 1.0551941788198604 1102 2 1.2689131384972328 1102 3 1.1324631002842764 1102 4 1.4375868705283068 1102 5 1.3665331777668754 1102 6 1.059106298470283 1102 7 1.0349377591993445 1102 8 1.1192115528497908 1102 9 1.5409092290356625 1102 10 1.7649421185018142 1102 11 1.3454719159504123 1102 12 1.2082683888216508 1102 13 1.1856028660749587 1102 14 2.2337743421744207 1102 15 1.7458846152070535 1102 16 1.7445667025614782 1102 17 2.089730276680122 1102 18 2.43675737332066 1102 19 1.8044939240159081 1102 20 1.5254203030300744 1102 21 1.4071675921089977 1102 22 1.6560963858330666 1102 23 1.8969688354809051 1102 24 1.7513691613443925 1102 25 2.0746272919156183 1103 1 0.9453468991507528 1103 2 1.188093563706623 1103 3 0.9851149340023149 1103 4 1.5068221137439508 1103 5 1.2721385975552622 1103 6 1.0246287110299903 1103 7 1.0487839161632593 1103 8 0.9607403976591016 1103 9 1.354405503235121 1103 10 1.7371625618690345 1103 11 1.3578492340630532 1103 12 1.2198601172810086 1103 13 1.2045455777119827 1103 14 2.077205098217071 1103 15 1.752614868058906 1103 16 1.8078383343952353 1103 17 2.1972365944300094 1103 18 2.437322069714021 1103 19 1.8015359808766718 1103 20 1.4902301828357736 1103 21 1.5569530824616322 1103 22 1.7723879730423349 1103 23 2.115276327004885 1103 24 1.7808979291450484 1103 25 2.0439385305188438 1104 1 0.973957036224391 1104 2 1.1635427433155243 1104 3 1.0797546258658137 1104 4 1.434334504380466 1104 5 1.3930559818061141 1104 6 1.1093073666484727 1104 7 1.0339667250053282 1104 8 1.0655938115053445 1104 9 1.443923765845554 1104 10 1.6538628658228731 1104 11 1.2127749308523477 1104 12 1.1584743354983402 1104 13 1.1903718265724095 1104 14 2.0959819124478756 1104 15 1.6780701389270547 1104 16 1.8355280821967952 1104 17 2.2067655573525187 1104 18 2.2973076740380023 1104 19 1.6879571427234623 1104 20 1.4259641491186201 1104 21 1.385380177194925 1104 22 1.7017805323661221 1104 23 2.014269137288558 1104 24 1.7432214602886376 1104 25 1.9398726149721242 1105 1 0.7467989193527735 1105 2 1.0242095001410334 1105 3 0.7839604754035889 1105 4 1.215052506846952 1105 5 1.1930246073345856 1105 6 0.9496250920375218 1105 7 0.7822101369700505 1105 8 0.8698413595068786 1105 9 1.1698594053132325 1105 10 1.455777050706459 1105 11 0.9350532004787979 1105 12 0.9540648235796638 1105 13 0.8456404435679943 1105 14 1.61116130036595 1105 15 1.4139901656205112 1105 16 1.6121147771669015 1105 17 1.7883575974538815 1105 18 1.5979180775632145 1105 19 1.3629112784382755 1105 20 1.1702461064026508 1105 21 1.115273548266174 1105 22 1.1812553088204965 1105 23 1.5504686161382608 1105 24 1.3730012875700623 1105 25 1.5146596750855323 1106 1 0.6353344613398058 1106 2 0.841722314855339 1106 3 0.7838050027667727 1106 4 1.2736044441302354 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1.6849879709746034 1107 18 1.9297412243245802 1107 19 1.2388849371578914 1107 20 1.1849142755042976 1107 21 1.0139278720448388 1107 22 1.3201194711148716 1107 23 1.7060115532809021 1107 24 1.4582073660734665 1107 25 1.754747548692599 1108 1 0.5143124163921335 1108 2 0.8166567559503584 1108 3 0.5424272241720622 1108 4 1.0456677728070503 1108 5 0.9207093985969621 1108 6 0.6308694941870954 1108 7 0.4703772141457492 1108 8 0.5197595686923293 1108 9 0.6470543846633845 1108 10 1.101448668360831 1108 11 0.6575817495159697 1108 12 0.49123299544611143 1108 13 0.48615581899716176 1108 14 1.4954615068644301 1108 15 0.928872672959919 1108 16 1.052586302767736 1108 17 1.353294217347397 1108 18 1.658721617892617 1108 19 0.9981755591766941 1108 20 0.862056451230238 1108 21 0.8516179031450122 1108 22 0.9967843678840396 1108 23 1.3384733556516224 1108 24 1.0456389946905276 1108 25 1.3627106901624089 1109 1 0.2679622837661384 1109 2 0.5163757783073102 1109 3 0.41482586001897204 1109 4 0.5675707663652148 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1.2428146681609533 1110 18 1.0608599967551342 1110 19 0.6374441067211052 1110 20 0.3432006518571953 1110 21 0.2622937502863749 1110 22 0.6269623795275692 1110 23 -0.6172965353768944 1110 24 0.15519825726703118 1110 25 0.6906824023582558 1111 1 0.13664860231376963 1111 2 0.5349464314984012 1111 3 0.39671899121867327 1111 4 0.7447841296442661 1111 5 0.6461208065530499 1111 6 0.34211983877496976 1111 7 0.3546705992571546 1111 8 0.396879533214765 1111 9 0.6137140097517744 1111 10 0.9087004452356879 1111 11 0.6037451381032213 1111 12 0.439947832500998 1111 13 0.1085938830725297 1111 14 1.1652399518442351 1111 15 0.884035048758463 1111 16 0.8791394951496443 1111 17 1.2547113724299166 1111 18 1.5312315959588503 1111 19 0.9634306141309708 1111 20 0.5332327244075188 1111 21 0.38077528092119195 1111 22 0.6939553014474953 1111 23 1.1451033761471052 1111 24 0.8123537538385861 1111 25 0.999058867742022 1112 1 0.07627034994054682 1112 2 0.22155046753060148 1112 3 0.11548386544004074 1112 4 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22 -0.09085377539313555 2209 23 0.37548122718393984 2209 24 -0.08008391060025133 2209 25 0.07410877211183475 2210 1 0.20413939621991872 2210 2 0.3474655068671453 2210 3 0.20955396895575618 2210 4 0.6134133130330852 2210 5 0.4321179883901607 2210 6 0.13157982399958001 2210 7 0.24798045740772068 2210 8 0.2744720006190562 2210 9 0.3388026478203656 2210 10 0.5254228048481053 2210 11 0.16477383886737584 2210 12 0.1339161652495875 2210 13 0.024099269814044533 2210 14 -5.503937889066478 2210 15 -1.9641364256142282 2210 16 -0.5248761017275569 2210 17 0.06273864414588814 2210 18 0.8941649098959203 2210 19 0.3909980517990803 2210 20 0.23699581331941744 2210 21 0.0043918710011467965 2210 22 0.4146102533419125 2210 23 0.7753778047417583 2210 24 0.5394925483608546 2210 25 0.40625773363112927 2211 1 -0.6697466004040109 2211 2 -0.5706235138816531 2211 3 -0.5039124097255296 2211 4 -0.7985957747220773 2211 5 -0.5414345771190341 2211 6 -0.7495733145573666 2211 7 -0.8444316706880066 2211 8 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2215 17 -4.906035444606339 2215 18 -3.3769178940725624 2215 19 -3.77001617036424 2215 20 -3.4978778443528036 2215 21 -3.629737417575793 2215 22 -9.72145039963507 2215 23 -11.071438232143102 2215 24 -7.152889616433605 2215 25 -5.37553794323534 2216 1 -2.296384229150128 2216 2 -2.3306494386736283 2216 3 -2.0894641044843816 2216 4 -7.389174950441319 2216 5 -3.8981789223608736 2216 6 -3.032533028915946 2216 7 -2.649868973014833 2216 8 -8.446516824224268 2216 9 -4.537889597114354 2216 10 -2.7186489241663025 2216 11 -2.6824516189882104 2216 12 -2.784370055351012 2216 13 -2.5823307527914388 2216 14 -2.0092931272120467 2216 15 -2.650184944375148 2216 16 -2.3975860420414534 2216 17 -2.0233951494065607 2216 18 -8.148245461670143 2216 19 -5.457932276476029 2216 20 -4.792160535368488 2216 21 -3.9361081669925753 2216 22 -3.5782135461738314 2216 23 -2.782432531238417 2216 24 -3.189283503525747 2216 25 -2.7287155785174484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109630.0 3 6.0 4 933.0 5 5256.0 6 6208.0 7 5535.0 8 5212.0 9 4911.0 10 4561.0 11 4396.0 12 4152.0 13 4136.0 14 4225.0 15 4280.0 16 4410.0 17 4667.0 18 5283.0 19 6476.0 20 8160.0 21 10316.0 22 12838.0 23 15256.0 24 17498.0 25 19994.0 26 22516.0 27 25591.0 28 28510.0 29 34019.0 30 40444.0 31 48293.0 32 61619.0 33 79831.0 34 104686.0 35 140004.0 36 198868.0 37 287203.0 38 778275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.239555503014536 18.06189856957087 12.154675493557159 25.54387043385743 2 15.02882731677579 20.048770490445406 40.251984022741894 24.670418170036914 3 18.565158986583008 24.571518639235023 29.48794288240512 27.37537949177685 4 11.880098161697397 16.35732641230759 38.218651671110884 33.54392375488413 5 13.21045223010128 37.615665471082096 35.64214629676435 13.531736002052277 6 32.47611820984434 36.48074181696616 17.868928859628287 13.174211113561213 7 28.391811857630618 31.343305429155986 22.08567868345293 18.179204029760466 8 25.90613831597069 35.30491377857187 20.589264356820397 18.199683548637044 9 26.786925094955777 14.825047170969501 19.542994048619626 38.8450336854551 10 14.303259587974784 28.343130528481908 34.93237975751057 22.421230126032743 11 34.985107074186985 22.266199008019704 23.79057543167764 18.958118486115673 12 23.25078200556093 24.42492368834623 31.027392861460346 21.296901444632496 13 29.025933273423504 21.23853728725597 25.559647662218133 24.17588177710239 14 21.253926459282876 20.703608281499978 28.178613995289194 29.863851263927955 15 23.87972010005063 28.930094912077863 23.22989163700664 23.960293350864863 16 23.231141318872893 27.3346875829289 26.36598359223677 23.068187505961436 17 22.411464217299475 27.395462978006325 27.389748249687347 22.803324555006856 18 22.362646625886814 26.96258740647796 28.297385326148294 22.377380641486926 19 23.845988205827577 26.694609511648725 26.929964073485024 22.529438209038673 20 24.177347773566517 26.58516567324738 26.848671280061126 22.38881527312498 21 24.11186882520732 26.13970379885524 26.442988835785936 23.305438540151503 22 23.93683948023683 26.47850630433879 26.58094944815782 23.00370476726656 23 23.3339047346502 26.361151132814914 26.941477643805417 23.36346648872946 24 23.535246379385427 26.474625232974773 26.761610480346043 23.228517907293753 25 23.56151768172888 26.182985114100042 26.851055992141454 23.40444121202962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 225.0 2 349.0 3 473.0 4 473.0 5 473.0 6 873.5 7 1274.0 8 1274.0 9 1274.0 10 2016.0 11 2758.0 12 2758.0 13 2758.0 14 5062.5 15 7367.0 16 7367.0 17 7367.0 18 13821.0 19 20275.0 20 20275.0 21 20275.0 22 32313.0 23 44351.0 24 44351.0 25 44351.0 26 63599.5 27 82848.0 28 82848.0 29 82848.0 30 107124.5 31 131401.0 32 131401.0 33 131401.0 34 155132.0 35 178863.0 36 178863.0 37 178863.0 38 201516.5 39 224170.0 40 224170.0 41 224170.0 42 241669.0 43 259168.0 44 259168.0 45 259168.0 46 271432.5 47 283697.0 48 283697.0 49 283697.0 50 278729.5 51 273762.0 52 273762.0 53 273762.0 54 250221.0 55 226680.0 56 226680.0 57 226680.0 58 195797.0 59 164914.0 60 164914.0 61 164914.0 62 136054.0 63 107194.0 64 107194.0 65 107194.0 66 83695.0 67 60196.0 68 60196.0 69 60196.0 70 44676.0 71 29156.0 72 29156.0 73 29156.0 74 20647.0 75 12138.0 76 12138.0 77 12138.0 78 8493.0 79 4848.0 80 4848.0 81 4848.0 82 3283.5 83 1719.0 84 1719.0 85 1719.0 86 1118.5 87 518.0 88 518.0 89 518.0 90 338.5 91 159.0 92 159.0 93 159.0 94 96.0 95 33.0 96 33.0 97 33.0 98 22.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1627798723254389 2 0.2570581220452479 3 0.2598435084916519 4 0.46454580733245904 5 0.2560667133100871 6 0.01751488765450633 7 0.01675952861819339 8 0.01590974970234133 9 0.01746767771473677 10 0.01959212500436692 11 0.01954491506459736 12 0.026720825909570308 13 0.0269568756084181 14 0.024265909041553246 15 0.0410726475995162 16 0.019828174703214713 17 0.04069496808135972 18 0.03063925091044369 19 0.025587787355100894 20 0.02813712410265707 21 0.03389673675454325 22 0.04371640422661149 23 0.02818433404242663 24 0.04796529880587179 25 0.035785134345325605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2118198.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.99014387504384 #Duplication Level Percentage of deduplicated Percentage of total 1 84.50643320276889 63.37149584242421 2 10.478684034012776 15.715960466634856 3 2.5558592559641156 5.749927599873344 4 0.9756558938054182 2.926583033960112 5 0.4482350055357606 1.6806603777478877 6 0.27819832804899836 1.2517279587714618 7 0.17043920737992124 0.8946882478358108 8 0.11018011167618535 0.660993794141243 9 0.08527642733169975 0.5755402399278498 >10 0.3690227448466422 4.602578537014479 >50 0.013862592080646073 0.7092914209832156 >100 0.006753358729820033 0.9401687320172711 >500 0.0010816928602152961 0.5543106438011705 >1k 3.1814495888676846E-4 0.3660731048671534 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2264 0.10688330363828123 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 4.7209939769558845E-5 0.0 12 0.0 0.0 0.0 4.7209939769558845E-5 4.7209939769558845E-5 13 0.0 4.7209939769558845E-5 0.0 9.441987953911769E-5 4.7209939769558845E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 435 0.0 12.048677 4 GGTCGGT 75 2.0962159E-4 11.387218 11 CTAGGAC 310 0.0 11.37381 3 GTAGGAC 790 0.0 11.2181635 3 GACACGC 70 0.0015075948 10.843433 7 TGTAGGA 905 0.0 10.431858 2 GTCTTAC 195 1.2732926E-11 10.285858 1 CTGTAGG 950 0.0 10.254923 1 TAGGACG 535 0.0 10.152827 4 GGTATCA 1225 0.0 10.135919 1 GTCTTAG 275 0.0 10.072125 1 GTATCAA 2300 0.0 10.049476 1 TGGTCGG 95 1.663576E-4 9.988552 10 TAGGACA 415 0.0 9.873844 4 GTATTGG 265 0.0 9.731365 1 CCTACAC 315 0.0 9.680669 3 AGGACGT 525 0.0 9.601493 5 GTTCTAG 220 1.4551915E-11 9.551154 1 GTACTAA 90 0.001060462 9.551154 1 AAACGAA 90 0.0011255936 9.489124 15 >>END_MODULE