FastQCFastQC Report
Thu 26 May 2016
SRR1513160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118198
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31090.14677570274355844No Hit
TATCAACGCAGAGTACTTTTTTTTT21560.10178463014316887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA759.809683E-713.9165759
GTGCCGT500.001477550413.3264746
GTCCTAG2400.010.714611
GTCTTAC1803.45608E-1110.5823311
GTAGGAC7400.010.4208293
GGTATCA12800.010.4169821
ATAGGAC2300.010.3481113
TGTAGGA8300.010.2099262
TAGGACC4300.010.1835024
TGCCGTC750.002667492710.1244927
GTATTAG1803.765308E-1010.0532141
ACCGTGC856.6611747E-410.0479128
CCTACAC3250.09.959663
GTATTAA2600.09.89040851
GTAATAC1752.4811015E-99.792273
GTCTTAT1502.5530426E-79.5240971
AGAGTCG800.00444136149.5193615
TAGGACA4600.09.5193614
AATCCCG900.00112570479.48902219
CTGTAGG8650.09.3589391
Produced by FastQC (version 0.11.5)