FastQCFastQC Report
Thu 26 May 2016
SRR1513159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93251
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33373.578513903336157No Hit
CCCATGTACTCTGCGTTGATACCAC30083.2257026734297756No Hit
GAGTACATGGGAAGCAGTGGTATCA12611.3522643188812988No Hit
CATGTACTCTGCGTTGATACCACTG9981.0702298098679908No Hit
GTATCAACGCAGAGTACTTTTTTTT9010.9662094776463523No Hit
TATCAACGCAGAGTACATGGGAAGC8840.9479791101435909No Hit
GCGTTGATACCACTGCTTCCCATGT8710.9340382408767735No Hit
GTATCAACGCAGAGTACATGGGAAG8260.8857813857224052No Hit
ACGCAGAGTACATGGGAAGCAGTGG6800.7292147001104545No Hit
GGTATCAACGCAGAGTACATGGGAA6590.7066948343717494No Hit
ACTCTGCGTTGATACCACTGCTTCC6510.6981158378998616No Hit
TATCAACGCAGAGTACTTTTTTTTT6490.6959710887818897No Hit
GGTATCAACGCAGAGTACTTTTTTT5540.5940955056782233No Hit
GTACTCTGCGTTGATACCACTGCTT3910.4192984525635114No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3900.41822607800452544No Hit
GCTTCCCATGTACTCTGCGTTGATA3580.38391009211697463No Hit
GGGAAGCAGTGGTATCAACGCAGAG3480.373186346527115No Hit
ATACCACTGCTTCCCATGTACTCTG3400.36460735005522726No Hit
GTGGTATCAACGCAGAGTACATGGG3280.35173885534739574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3220.34530460799347995No Hit
CTGCTTCCCATGTACTCTGCGTTGA3120.33458086240362034No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3010.32278474225477477No Hit
GCAGAGTACATGGGAAGCAGTGGTA2970.3184952440188309No Hit
GATACCACTGCTTCCCATGTACTCT2970.3184952440188309No Hit
GCAGTGGTATCAACGCAGAGTACAT2750.2949030037211397No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2700.2895411309262099No Hit
TACCACTGCTTCCCATGTACTCTGC2630.2820345090133082No Hit
CAGTGGTATCAACGCAGAGTACATG2600.27881738533635025No Hit
CCACTGCTTCCCATGTACTCTGCGT2580.27667263621837834No Hit
AAGCAGTGGTATCAACGCAGAGTAC2410.258442268715617No Hit
CATGGGAAGCAGTGGTATCAACGCA2370.25415277047967316No Hit
GTTGATACCACTGCTTCCCATGTAC2320.24879089768474333No Hit
ACATGGGAAGCAGTGGTATCAACGC1900.2037511662073329No Hit
CTTCCCATGTACTCTGCGTTGATAC1820.1951721697354452No Hit
ATGGGAAGCAGTGGTATCAACGCAG1750.18766554782254347No Hit
CCCATGTACTCTGCGTTTATACCAC1690.18123130046862768No Hit
GTACATGGGAAGCAGTTGTATCAAC1630.17479705311471191No Hit
CCATGTACTCTGCGTTGATACCACT1570.16836280576079612No Hit
GAACAAAAAAAAAAAAAAAAAAAAA1530.16407330752485227No Hit
GTACATGGGAAGCAGTGTTATCAAC1510.16192855840688036No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1470.1576390601709365No Hit
TGATACCACTGCTTCCCATGTACTC1460.15656668561195053No Hit
TTCCCATGTACTCTGCGTTGATACC1370.14691531458107687No Hit
ATCAACGCAGAGTACATGGGAAGCA1350.14477056546310496No Hit
GTACATGGTAAGCAGTGGTATCAAC1170.12546782340135762No Hit
AAAAAGTACTCTGCGTTGATACCAC1160.12439544884237166No Hit
ATTCCATTCCATTCCATTCCATTCC1080.11581645237048396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTGT402.7564872E-416.60107213
TTGTATC402.7564872E-416.60107216
AGCAGTT402.7564872E-416.60107211
GCAGTTG402.7564872E-416.60107212
TGTATCA350.002170480616.26227617
GTGTTAT508.680842E-515.178122515
GCAGTGT508.680842E-515.178122512
AGTGTTA508.680842E-515.178122514
AGTTGTA456.751739E-414.75650814
TTATACC400.00528024714.2294917
GCGTTTA400.00528024714.2294913
CAGTGTT551.9529674E-413.79829413
TGTTATC604.077567E-412.64843616
GTTATCA604.077567E-412.64843617
GTTGTAT550.003064308512.07350715
ATGGGAG803.638894E-410.7065625
GAAGCAG9450.010.3395959
GGAAGCA9450.010.3395958
GGGAAGC10100.09.7680997
AAGCAGT10400.09.57754110
Produced by FastQC (version 0.11.5)