##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513159_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93251
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.744077811498	31.0	27.0	34.0	2.0	34.0
2	26.470665193938938	31.0	26.0	34.0	2.0	34.0
3	26.6602181209853	31.0	26.0	34.0	2.0	34.0
4	29.262292093382378	35.0	28.0	37.0	2.0	37.0
5	29.608304468584787	35.0	30.0	37.0	2.0	37.0
6	29.635274688743284	35.0	30.0	37.0	2.0	37.0
7	29.518535994252073	35.0	30.0	37.0	2.0	37.0
8	29.194432231289746	35.0	30.0	37.0	2.0	37.0
9	29.97251504005319	37.0	27.0	39.0	2.0	39.0
10	29.669794425797043	37.0	27.0	39.0	2.0	39.0
11	30.009683542267645	37.0	28.0	39.0	2.0	39.0
12	30.02673429775552	37.0	29.0	39.0	2.0	39.0
13	29.93730898328168	37.0	28.0	39.0	2.0	39.0
14	30.090369004085748	38.0	25.0	41.0	2.0	41.0
15	30.370794951260578	38.0	27.0	41.0	2.0	41.0
16	30.197349090090185	38.0	27.0	40.0	2.0	41.0
17	29.989533624304297	38.0	27.0	40.0	2.0	41.0
18	29.79918714008429	38.0	25.0	40.0	2.0	41.0
19	30.30708517871122	38.0	27.0	41.0	2.0	41.0
20	30.548734061833116	38.0	28.0	41.0	2.0	41.0
21	30.576326259235827	38.0	29.0	41.0	2.0	41.0
22	30.343234925094638	38.0	27.0	41.0	2.0	41.0
23	29.967571393336264	38.0	27.0	41.0	2.0	41.0
24	30.182024857642276	38.0	28.0	41.0	2.0	41.0
25	29.90530932644154	38.0	27.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	2.4480617022803806
1101	2	2.3532789092605206
1101	3	2.3334882931717473
1101	4	2.4973368455350844
1101	5	3.0572661996091846
1101	6	1.8683151761625183
1101	7	1.5467540810529457
1101	8	2.1347996860164855
1101	9	2.327612985892351
1101	10	2.671985749842719
1101	11	1.5927129969244618
1101	12	1.8876900608683123
1101	13	1.6990566508368694
1101	14	2.896878100381663
1101	15	2.221600766884297
1101	16	2.6283999078028053
1101	17	3.1256438638455784
1101	18	2.8525318311413628
1101	19	2.872592670674372
1101	20	2.1233402434860835
1101	21	1.7985792234975477
1101	22	2.0326619802753285
1101	23	2.499967141039903
1101	24	1.9266059916511686
1101	25	2.164899467382206
1102	1	2.5187835189876004
1102	2	2.8303267600309994
1102	3	2.676445849267992
1102	4	2.807150308633215
1102	5	3.1358820417286744
1102	6	3.2050007668627103
1102	7	2.861271518198123
1102	8	3.3290684072925067
1102	9	3.7712464003286854
1102	10	3.370120380824069
1102	11	3.096741112631406
1102	12	2.687419717532279
1102	13	2.3119249936322213
1102	14	3.89893270973554
1102	15	3.0835093908367135
1102	16	2.640781632593292
1102	17	3.096771195556858
1102	18	3.852369625139737
1102	19	3.565807052939853
1102	20	3.7355597622749457
1102	21	3.2999579745113294
1102	22	3.7963819045791993
1102	23	3.9256497579633916
1102	24	3.8541539775933167
1102	25	3.947002738536572
1103	1	-0.27367032386340995
1103	2	0.3104684517441818
1103	3	0.2378455916466926
1103	4	0.5945590677573058
1103	5	0.36892197302966423
1103	6	0.5636310803019526
1103	7	0.3117867607915059
1103	8	0.6113247404827149
1103	9	0.5032665806635883
1103	10	1.4206785603002352
1103	11	0.736285981673916
1103	12	0.8750538952909714
1103	13	0.8055381323183504
1103	14	1.52291295876946
1103	15	0.9976356252720926
1103	16	0.5794892324215404
1103	17	0.74345432136192
1103	18	1.245832484736134
1103	19	0.9386602088007372
1103	20	0.8692008099348847
1103	21	1.5180236679419892
1103	22	1.3548297362666162
1103	23	1.3798146355932772
1103	24	1.4857018522176197
1103	25	1.040444129691572
1104	1	1.3727981175277435
1104	2	1.88872092061494
1104	3	1.5815553383190846
1104	4	2.062624761187962
1104	5	1.8220134285899903
1104	6	2.3509861683225637
1104	7	1.4890087444754911
1104	8	2.197681545978636
1104	9	2.107012823686347
1104	10	2.0343539574664007
1104	11	2.1113396527773958
1104	12	1.709317527751253
1104	13	2.4287133148001026
1104	14	3.380684534553062
1104	15	3.1711006317126227
1104	16	2.8451611946370825
1104	17	3.1624646262137865
1104	18	3.100544807621489
1104	19	2.974566177027441
1104	20	2.7063626819829736
1104	21	2.445943375971183
1104	22	2.927768765893063
1104	23	2.7577665462845644
1104	24	2.6132780651845557
1104	25	2.866710767733654
1105	1	1.3432307844059856
1105	2	1.3716363971832237
1105	3	1.6014432046049194
1105	4	1.5818779083370167
1105	5	1.4062195007363982
1105	6	1.5109882840530169
1105	7	1.4390246124539132
1105	8	1.1813375282065017
1105	9	1.0933134689841353
1105	10	1.0449326097461018
1105	11	0.8577693576329963
1105	12	1.0677222669874737
1105	13	0.6298134946371903
1105	14	1.0231260874992145
1105	15	0.9652400583496714
1105	16	1.3606060269654456
1105	17	1.5898950715750502
1105	18	1.34142072003025
1105	19	1.1693720587581105
1105	20	1.1328410486390617
1105	21	1.1720091751883643
1105	22	1.209795635669856
1105	23	1.775168429284669
1105	24	1.6375560721664684
1105	25	1.3621619472533801
1106	1	1.1868723439079254
1106	2	1.2141552785875938
1106	3	1.5936865195671572
1106	4	1.966037940996742
1106	5	2.1466767369070467
1106	6	2.1425457447592855
1106	7	2.316550638173446
1106	8	2.1253056849731884
1106	9	1.709459621260109
1106	10	1.7054755776987456
1106	11	1.801737514399683
1106	12	1.35227013389909
1106	13	1.5889888365437024
1106	14	2.5164920805590185
1106	15	2.4102288315686984
1106	16	2.582181858767857
1106	17	3.007021015645268
1106	18	2.9400122693260258
1106	19	2.167637010789136
1106	20	2.0392505199597366
1106	21	1.7979532454067808
1106	22	2.2770542598788808
1106	23	2.497254569313249
1106	24	2.322797958265671
1106	25	2.3375650907520544
1107	1	0.1757178255569407
1107	2	0.2609836943375683
1107	3	0.15819767408551044
1107	4	0.7998510385602238
1107	5	0.7928163482980217
1107	6	0.0955117157678167
1107	7	0.5620014452054178
1107	8	1.1611851956136725
1107	9	1.245698955073209
1107	10	1.6109962932328266
1107	11	1.228127973945277
1107	12	1.169171542349794
1107	13	0.7425819279387866
1107	14	1.89893270973554
1107	15	0.4776699747783191
1107	16	1.1590298077757701
1107	17	1.257355137162694
1107	18	1.45090977112514
1107	19	0.8577778558595597
1107	20	1.0713261856326106
1107	21	1.1028776825405302
1107	22	0.8985716856010946
1107	23	1.37090523241595
1107	24	1.4088985031407617
1107	25	1.7937180670037236
1108	1	1.3673360786976154
1108	2	1.2045260747933995
1108	3	1.1628155796418937
1108	4	1.7875862786416583
1108	5	1.544492201120903
1108	6	0.8598495676969087
1108	7	0.30548423978310524
1108	8	1.098669678457906
1108	9	1.3865699019921216
1108	10	0.9840038904322874
1108	11	1.1866661337347395
1108	12	1.706586508336187
1108	13	1.4594496969442012
1108	14	2.8063263241156022
1108	15	2.014042187897143
1108	16	2.2010378518693194
1108	17	1.7737164262038583
1108	18	2.8108084751322906
1108	19	2.2863492844309903
1108	20	1.2066357839244795
1108	21	1.3437039400508333
1108	22	1.9767284957704163
1108	23	1.832695788054263
1108	24	1.9364321443344643
1108	25	2.170946330571926
1109	1	-0.3212869567234513
1109	2	-0.31721917503066877
1109	3	-0.2670225625115208
1109	4	-0.03216507017372905
1109	5	0.23555483307023195
1109	6	-0.3618253091503796
1109	7	-0.7191463020888165
1109	8	-0.8784825617485197
1109	9	-0.44556709073600587
1109	10	-0.009240303797128036
1109	11	-0.6399817668047838
1109	12	-1.0997939748915861
1109	13	-0.8056687642333742
1109	14	-0.3416915037051851
1109	15	-0.6238653889266885
1109	16	-0.40965884060076974
1109	17	-0.16247874151840236
1109	18	0.3414207200302499
1109	19	-0.32842904069216416
1109	20	-0.6396227194768791
1109	21	-0.7983556424028393
1109	22	-0.7318335497374413
1109	23	-0.020083944528426656
1109	24	-0.5053224885531726
1109	25	-0.6299919757850994
1110	1	-0.2808271866085086
1110	2	0.17772335370496606
1110	3	0.3794142190976757
1110	4	0.6062257344239725
1110	5	0.7491180514610392
1110	6	0.0046114724588157685
1110	7	0.2578651921640578
1110	8	0.4488737600905601
1110	9	-0.07683145855209617
1110	10	0.6208746387316069
1110	11	-0.1480277438162787
1110	12	-0.6774951243168736
1110	13	-0.0394618676816485
1110	14	0.7842855077890682
1110	15	0.3756748409583679
1110	16	0.5098813892842884
1110	17	0.9345327527344693
1110	18	0.7747540533635835
1110	19	0.6644445225262281
1110	20	0.06037728052312019
1110	21	-0.42364299872467726
1110	22	-0.3458565382431864
1110	23	-1.6007735997008403
1110	24	-0.7030236379784611
1110	25	-0.6210264585437208
1111	1	0.7665321207507994
1111	2	0.2972904532720655
1111	3	0.21569127537473065
1111	4	1.245533093731332
1111	5	0.377516319859307
1111	6	0.7429664508137925
1111	7	0.2860037203025847
1111	8	0.4032460544628549
1111	9	0.9437746020539635
1111	10	1.4097923876493574
1111	11	0.9822752864867503
1111	12	0.8250156981939512
1111	13	0.4337848855651032
1111	14	0.7454110489146082
1111	15	0.7493544946380233
1111	16	0.7427818221847211
1111	17	0.8621518003535193
1111	18	1.6076544862640176
1111	19	0.4097259078076121
1111	20	0.20323442338026254
1111	21	0.266400291318611
1111	22	0.8539703015836508
1111	23	0.5228627639355246
1111	24	0.35325341829859624
1111	25	0.9987138011965371
1112	1	-1.0773568669738047
1112	2	-0.9141487924137586
1112	3	-0.9958369224548385
1112	4	-1.390029973338585
1112	5	-0.769877382328918
1112	6	-1.294749258134793
1112	7	-1.2238699676532967
1112	8	-1.3386148243843259
1112	9	-1.310895385492735
1112	10	-1.0879838087569773
1112	11	-1.1758816250948172
1112	12	-1.7897325672392483
1112	13	-1.2920646074076778
1112	14	-0.7774496520282845
1112	15	-0.6212201362105816
1112	16	-0.5850957796654832
1112	17	-0.043914735850005826
1112	18	-0.5284422936683804
1112	19	-0.7244139249623593
1112	20	-0.8985268290659221
1112	21	-0.8980722224689686
1112	22	-0.6114273144988971
1112	23	0.364705852353957
1112	24	-0.3993706699419306
1112	25	0.18417902090833493
1113	1	0.7823020650921713
1113	2	0.9800362788750334
1113	3	1.3600944912065174
1113	4	1.325001244628055
1113	5	1.680546622889608
1113	6	1.2680128330030342
1113	7	0.883715532300112
1113	8	1.0918669573694721
1113	9	1.75391684076763
1113	10	1.8751603530173213
1113	11	1.6764620521020888
1113	12	1.9514844675198617
1113	13	1.649925887420391
1113	14	2.2348977526870257
1113	15	1.8167632763325194
1113	16	2.2962759471074143
1113	17	2.2567096234827666
1113	18	1.7087676588057619
1113	19	1.8033560871520784
1113	20	1.8664997295027135
1113	21	2.1328195863093384
1113	22	1.9359121692398062
1113	23	1.9755529309114053
1113	24	1.6167042531780034
1113	25	2.211762657102536
1114	1	0.4755126826725373
1114	2	0.7140632229860095
1114	3	0.14961029752904764
1114	4	0.7326309631821424
1114	5	-1.551012667493211
1114	6	0.39859840056339024
1114	7	0.29708087843856745
1114	8	0.6938410803519979
1114	9	1.3276129858923476
1114	10	2.1804824818688644
1114	11	1.3016454587980988
1114	12	1.03191664038901
1114	13	1.4419760408150815
1114	14	-4.459113185021387
1114	15	-0.7409264662311763
1114	16	0.04844348078755445
1114	17	1.075970661231203
1114	18	-4.978840717878246
1114	19	-1.5278430591731187
1114	20	-0.19779265411740354
1114	21	0.309690334608657
1114	22	0.8278689519528903
1114	23	0.8324290146782474
1114	24	1.4930547933940908
1114	25	1.2324375937438603
1115	1	1.2288118792089016
1115	2	1.3463433112421193
1115	3	1.0904120959554255
1115	4	-1.2740715054911007
1115	5	1.2449991554950088
1115	6	1.023634827023571
1115	7	1.030688971357261
1115	8	1.7192771571181602
1115	9	2.165546460768496
1115	10	2.284016889262393
1115	11	1.615454209474592
1115	12	1.978088739802022
1115	13	1.842958514483957
1115	14	-3.0133365728903385
1115	15	0.20820138023649903
1115	16	1.0402423234668774
1115	17	1.4153820096346834
1115	18	2.3668984270366167
1115	19	1.819561295350006
1115	20	1.6113326945358608
1115	21	1.8723230309993113
1115	22	0.5481137377653056
1115	23	1.872602196477505
1115	24	1.8044073598983985
1115	25	2.4873811847683776
1116	1	-1.0745824608701113
1116	2	-0.5575509078984098
1116	3	-0.9713874686660375
1116	4	-0.37537764110345506
1116	5	-1.3170422860917057
1116	6	-1.113025658342874
1116	7	-0.4531052719709656
1116	8	-0.317286577462184
1116	9	-0.23286521382635783
1116	10	0.01952442776114438
1116	11	-0.20414588727619432
1116	12	-1.0839930146122327
1116	13	-0.3257909816056994
1116	14	0.18318846137556832
1116	15	-0.7566458341471183
1116	16	-0.7702873870870199
1116	17	-0.5356782177296644
1116	18	-0.6965539635140523
1116	19	-0.27513353654550343
1116	20	-0.8003822131477669
1116	21	-0.6734320282605424
1116	22	-0.3487257365554228
1116	23	-1.4610267642578023
1116	24	-1.3085088700459693
1116	25	-0.5458365851260005
1201	1	1.9643811467248256
1201	2	2.062306687038298
1201	3	1.7088933857643411
1201	4	1.8523715677573058
1201	5	2.3174513847943707
1201	6	2.1656531391254816
1201	7	2.01307352549739
1201	8	1.9265820934238924
1201	9	2.083689374781237
1201	10	2.8379579720649417
1201	11	2.587388922850387
1201	12	1.637921542349794
1201	13	1.6727256323183504
1201	14	3.006368841122402
1201	15	2.5904665076250346
1201	16	3.1718605559509534
1201	17	3.296303586067804
1201	18	2.70860822003025
1201	19	2.9202778558595597
1201	20	2.91975228052312
1201	21	2.853961167941989
1201	22	2.909976795090145
1201	23	3.5811014002991612
1201	24	3.5339555286882067
1201	25	3.6217860414562786
1202	1	0.7995484002459534
1202	2	1.270493306756606
1202	3	0.5514329984403972
1202	4	0.9801224480389976
1202	5	1.6255499763436667
1202	6	1.4383203926466095
1202	7	0.5348604973283742
1202	8	0.9140380793393845
1202	9	0.5615722973164488
1202	10	1.2758981129100135
1202	11	0.872160049610951
1202	12	0.35931238742021776
1202	13	0.6453268647127182
1202	14	1.8897315171787419
1202	15	1.7130457329771467
1202	16	1.8568297460917975
1202	17	1.78871115649034
1202	18	1.975223536931658
1202	19	1.50566517980322
1202	20	0.8702364354526964
1202	21	1.4810518369560732
1202	22	1.6573359500197213
1202	23	1.7296489355104292
1202	24	1.4143472540403188
1202	25	1.6755932597661385
1203	1	1.6613004729659586
1203	2	1.8431134246269494
1203	3	2.0565773396650506
1203	4	2.719559067757306
1203	5	2.1162102500425988
1203	6	2.5518455150120047
1203	7	2.059283631880369
1203	8	3.2117106395231865
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2211	15	-0.8863071410236145
2211	16	-5.989668160265264
2211	17	-1.8183501301484135
2211	18	-1.2261468475373185
2211	19	-1.1692491711674684
2211	20	-0.8382713681255289
2211	21	-0.4515709266526038
2211	22	-1.0376583400449917
2211	23	-0.6583411672684072
2211	24	-1.1241948091496319
2211	25	-7.07859402611129
2212	1	-3.7941605199418404
2212	2	-6.035609979628369
2212	3	-4.313919114235659
2212	4	-6.607107598909362
2212	5	-3.930881948538964
2212	6	-4.0220551942078515
2212	7	-4.42880147450261
2212	8	-4.344459573242773
2212	9	-4.3634981252187615
2212	10	-3.5057920279350583
2212	11	-4.1413610771496145
2212	12	-3.844161790983538
2212	13	-4.879461867681648
2212	14	-8.209047825544264
2212	15	-8.284325159041632
2212	16	-6.61678527738238
2212	17	-9.165467247265529
2212	18	-6.205245946636417
2212	19	-5.8888888108071065
2212	20	-5.052956052810213
2212	21	-4.89697633205801
2212	22	-4.792523204909855
2212	23	-4.447440266367504
2212	24	-4.696356971311793
2212	25	-4.754359791877054
2213	1	-0.004791150572472702
2213	2	0.14592155190316092
2213	3	0.4004052100886639
2213	4	0.9290185272167655
2213	5	0.5167757091186935
2213	6	-0.08899213114478854
2213	7	0.26011744441630924
2213	8	0.5030179042347029
2213	9	0.28406944234880527
2213	10	-2.7963325684755986
2213	11	-1.002982698771234
2213	12	-0.6010987279204763
2213	13	-0.8870294352492181
2213	14	-0.15157034806678737
2213	15	-0.7849557896722636
2213	16	-0.05723572783282904
2213	17	0.31002824822996544
2213	18	0.2265558551653868
2213	19	-0.28411403603233154
2213	20	-0.9058389356930974
2213	21	-0.49886822394990205
2213	22	-1.2133340157206653
2213	23	-1.8340168429440808
2213	24	-0.7660867010415231
2213	25	-0.5718372693545319
2214	1	-1.6766296557443106
2214	2	-1.3701778808629363
2214	3	-1.3467586204084974
2214	4	-0.6322927840945454
2214	5	-3.8723634300204424
2214	6	-1.8218082806276037
2214	7	-1.476702709070512
2214	8	-0.6565583386748735
2214	9	-0.36374503879901
2214	10	-4.987273509416539
2214	11	-1.8875339166557872
2214	12	-1.330828457650206
2214	13	-0.2330421145952286
2214	14	-0.45003547986525305
2214	15	-0.10802886274533563
2214	16	-0.5234519440490466
2214	17	0.012063616932000087
2214	18	0.21179109040062016
2214	19	-0.389135724387355
2214	20	-0.6248079046620667
2214	21	-0.40018620860121956
2214	22	-0.3870911061444211
2214	23	-0.1802797725403451
2214	24	-0.8882088231636445
2214	25	-1.2548536190375472
2215	1	-1.3284088859549144
2215	2	-3.3251524632885
2215	3	-1.582415846261803
2215	4	-1.1627938734191652
2215	5	-2.0738884844866767
2215	6	-1.7844081353843215
2215	7	-2.2715465725418262
2215	8	-2.0512569588636893
2215	9	-8.450164791885431
2215	10	-3.7737658841442077
2215	11	-2.789857809175757
2215	12	-2.9615474118985716
2215	13	-2.2900501029757656
2215	14	-5.779374622929886
2215	15	-3.6232794074076438
2215	16	-8.12149115973532
2215	17	-3.878277704781869
2215	18	-2.5213243780089662
2215	19	-2.606274431722138
2215	20	-2.969034484182764
2215	21	-2.3242965934959194
2215	22	-7.302850002295475
2215	23	-9.187178828459007
2215	24	-5.912173964775846
2215	25	-4.859065674229996
2216	1	-1.6105241563054769
2216	2	-1.5477311917495804
2216	3	-1.1890706293871744
2216	4	-6.504683356485117
2216	5	-3.7090637667207815
2216	6	-2.2293279214805786
2216	7	-1.8815287472298827
2216	8	-6.366883815667016
2216	9	-3.165316307036946
2216	10	-1.893670815813845
2216	11	-1.3298459256344621
2216	12	-1.99446482128657
2216	13	-1.0558255040452877
2216	14	-1.2169266134230519
2216	15	-1.9043251590416332
2216	16	-1.6113307319278327
2216	17	-1.2884975502958333
2216	18	-6.282821704212175
2216	19	-5.0088888108071075
2216	20	-4.209319689173849
2216	21	-3.732733907815586
2216	22	-3.485856538243187
2216	23	-2.4044099633372014
2216	24	-2.745447880402704
2216	25	-2.6276931252103886
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11023.0
3	2.0
4	73.0
5	477.0
6	867.0
7	659.0
8	620.0
9	450.0
10	416.0
11	425.0
12	436.0
13	357.0
14	347.0
15	282.0
16	277.0
17	261.0
18	306.0
19	436.0
20	604.0
21	753.0
22	889.0
23	1000.0
24	1071.0
25	1149.0
26	1216.0
27	1245.0
28	1246.0
29	1340.0
30	1655.0
31	1941.0
32	2345.0
33	3110.0
34	4035.0
35	5316.0
36	7754.0
37	11583.0
38	27285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.434051418630126	17.717304924933956	10.892630855473701	21.95601280096222
2	13.744514241459427	23.79098184321487	36.90409603304363	25.560407882282078
3	20.872418244406195	31.841652323580032	26.25	21.03592943201377
4	12.10020804812056	19.14905084782304	32.58809706037707	36.16264404367933
5	11.23433550260851	42.16210401764105	32.38207927714731	14.221481202603131
6	31.345316488277813	32.565796529460975	19.271358400720707	16.817528581540508
7	25.406187999356533	34.13587859938871	21.750227894257065	18.707705506997694
8	29.56373463741072	32.08823971001437	21.02932029255947	17.318705360015443
9	30.18103041482562	14.41165115181674	18.535240873407403	36.87207755995024
10	17.166972319637935	31.299936724472616	29.786686400051476	21.746404555837973
11	29.951416222477235	28.165721088362417	17.9835051104128	23.899357578747544
12	25.545652477020926	30.91154799060459	26.697555691410063	16.845243840964425
13	31.01162641039942	22.73370800978163	21.715860826290275	24.53880475352868
14	22.075637642919965	27.522148572409204	25.50249908831542	24.899714696355407
15	32.4507858177332	25.035670224749236	21.58558171968031	20.927962237837257
16	19.31297791791341	29.54430895616829	29.755584869641687	21.38712825627661
17	23.65296117910816	29.926091200669365	26.689765401242184	19.731182218980294
18	24.04457744741556	25.68029947120593	29.78837511128273	20.486747970095784
19	22.446506140397922	30.70520727194723	30.18823403228401	16.66005255537084
20	25.18582077545986	26.811819595645414	27.288035608945137	20.71432401994959
21	22.115075193615517	28.543539355973657	30.043121017741832	19.298264432669
22	22.2178120105559	28.226308223733614	23.416078439786308	26.13980132592418
23	18.798734380865557	30.0874135249638	27.742800450474608	23.371051643696035
24	21.251166732826228	34.12224141445569	24.75404735594202	19.87254449677606
25	21.875737486859325	25.82652163652357	23.94284610928751	28.354894767329604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	385.0
1	385.0
2	348.5
3	312.0
4	312.0
5	312.0
6	588.5
7	865.0
8	865.0
9	865.0
10	811.5
11	758.0
12	758.0
13	758.0
14	683.0
15	608.0
16	608.0
17	608.0
18	861.0
19	1114.0
20	1114.0
21	1114.0
22	1525.0
23	1936.0
24	1936.0
25	1936.0
26	2624.0
27	3312.0
28	3312.0
29	3312.0
30	3997.0
31	4682.0
32	4682.0
33	4682.0
34	5069.5
35	5457.0
36	5457.0
37	5457.0
38	6132.0
39	6807.0
40	6807.0
41	6807.0
42	7968.5
43	9130.0
44	9130.0
45	9130.0
46	15082.5
47	21035.0
48	21035.0
49	21035.0
50	18587.5
51	16140.0
52	16140.0
53	16140.0
54	12184.5
55	8229.0
56	8229.0
57	8229.0
58	6637.0
59	5045.0
60	5045.0
61	5045.0
62	4249.5
63	3454.0
64	3454.0
65	3454.0
66	2767.0
67	2080.0
68	2080.0
69	2080.0
70	1590.5
71	1101.0
72	1101.0
73	1101.0
74	811.5
75	522.0
76	522.0
77	522.0
78	357.5
79	193.0
80	193.0
81	193.0
82	125.0
83	57.0
84	57.0
85	57.0
86	36.0
87	15.0
88	15.0
89	15.0
90	10.5
91	6.0
92	6.0
93	6.0
94	6.0
95	6.0
96	6.0
97	6.0
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14262581634513302
2	0.3034820001930274
3	0.3120609966649151
4	0.5190292865492059
5	0.3066991238699853
6	0.009651371030873664
7	0.006434247353915776
8	0.005361872794929813
9	0.007506621912901738
10	0.008578996471887701
11	0.010723745589859626
12	0.015013243825803477
13	0.01608561838478944
14	0.018230367502761365
15	0.038605484123494654
16	0.008578996471887701
17	0.030026487651606953
18	0.021447491179719252
19	0.017157992943775402
20	0.017157992943775402
21	0.026809363974649066
22	0.03538836044653677
23	0.017157992943775402
24	0.04503973147741043
25	0.031098862210592916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	93251.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.43427952515255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.56233443044071	45.71961694780753
2	8.162077546760857	8.72269466279182
3	2.8417757084370234	4.5554471265723695
4	1.1158384843862887	2.384961019184781
5	0.6321746809023039	1.688989930402891
6	0.3351529260656659	1.0745193081039346
7	0.2167456048807899	0.8107151665933877
8	0.17460062615396965	0.74637269305423
9	0.11439351368708356	0.5501281487597989
>10	0.6984025046158786	6.970434633408757
>50	0.05217949747130128	1.8487737396917996
>100	0.06823472746247089	8.54253573688218
>500	0.02006903748896203	8.22832999109929
>1k	0.0060207112466886085	8.156480895647231
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3337	3.578513903336157	No Hit
CCCATGTACTCTGCGTTGATACCAC	3008	3.2257026734297756	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1261	1.3522643188812988	No Hit
CATGTACTCTGCGTTGATACCACTG	998	1.0702298098679908	No Hit
GTATCAACGCAGAGTACTTTTTTTT	901	0.9662094776463523	No Hit
TATCAACGCAGAGTACATGGGAAGC	884	0.9479791101435909	No Hit
GCGTTGATACCACTGCTTCCCATGT	871	0.9340382408767735	No Hit
GTATCAACGCAGAGTACATGGGAAG	826	0.8857813857224052	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	680	0.7292147001104545	No Hit
GGTATCAACGCAGAGTACATGGGAA	659	0.7066948343717494	No Hit
ACTCTGCGTTGATACCACTGCTTCC	651	0.6981158378998616	No Hit
TATCAACGCAGAGTACTTTTTTTTT	649	0.6959710887818897	No Hit
GGTATCAACGCAGAGTACTTTTTTT	554	0.5940955056782233	No Hit
GTACTCTGCGTTGATACCACTGCTT	391	0.4192984525635114	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	390	0.41822607800452544	No Hit
GCTTCCCATGTACTCTGCGTTGATA	358	0.38391009211697463	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	348	0.373186346527115	No Hit
ATACCACTGCTTCCCATGTACTCTG	340	0.36460735005522726	No Hit
GTGGTATCAACGCAGAGTACATGGG	328	0.35173885534739574	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	322	0.34530460799347995	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	312	0.33458086240362034	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	301	0.32278474225477477	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	297	0.3184952440188309	No Hit
GATACCACTGCTTCCCATGTACTCT	297	0.3184952440188309	No Hit
GCAGTGGTATCAACGCAGAGTACAT	275	0.2949030037211397	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	270	0.2895411309262099	No Hit
TACCACTGCTTCCCATGTACTCTGC	263	0.2820345090133082	No Hit
CAGTGGTATCAACGCAGAGTACATG	260	0.27881738533635025	No Hit
CCACTGCTTCCCATGTACTCTGCGT	258	0.27667263621837834	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	241	0.258442268715617	No Hit
CATGGGAAGCAGTGGTATCAACGCA	237	0.25415277047967316	No Hit
GTTGATACCACTGCTTCCCATGTAC	232	0.24879089768474333	No Hit
ACATGGGAAGCAGTGGTATCAACGC	190	0.2037511662073329	No Hit
CTTCCCATGTACTCTGCGTTGATAC	182	0.1951721697354452	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	175	0.18766554782254347	No Hit
CCCATGTACTCTGCGTTTATACCAC	169	0.18123130046862768	No Hit
GTACATGGGAAGCAGTTGTATCAAC	163	0.17479705311471191	No Hit
CCATGTACTCTGCGTTGATACCACT	157	0.16836280576079612	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	153	0.16407330752485227	No Hit
GTACATGGGAAGCAGTGTTATCAAC	151	0.16192855840688036	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	147	0.1576390601709365	No Hit
TGATACCACTGCTTCCCATGTACTC	146	0.15656668561195053	No Hit
TTCCCATGTACTCTGCGTTGATACC	137	0.14691531458107687	No Hit
ATCAACGCAGAGTACATGGGAAGCA	135	0.14477056546310496	No Hit
GTACATGGTAAGCAGTGGTATCAAC	117	0.12546782340135762	No Hit
AAAAAGTACTCTGCGTTGATACCAC	116	0.12439544884237166	No Hit
ATTCCATTCCATTCCATTCCATTCC	108	0.11581645237048396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0010723745589859626	0.0
11	0.0	0.0	0.0	0.0010723745589859626	0.0
12	0.0	0.0	0.0	0.0010723745589859626	0.0
13	0.0	0.0	0.0	0.0010723745589859626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTGT	40	2.7564872E-4	16.601072	13
TTGTATC	40	2.7564872E-4	16.601072	16
AGCAGTT	40	2.7564872E-4	16.601072	11
GCAGTTG	40	2.7564872E-4	16.601072	12
TGTATCA	35	0.0021704806	16.262276	17
GTGTTAT	50	8.680842E-5	15.1781225	15
GCAGTGT	50	8.680842E-5	15.1781225	12
AGTGTTA	50	8.680842E-5	15.1781225	14
AGTTGTA	45	6.751739E-4	14.756508	14
TTATACC	40	0.005280247	14.22949	17
GCGTTTA	40	0.005280247	14.22949	13
CAGTGTT	55	1.9529674E-4	13.798294	13
TGTTATC	60	4.077567E-4	12.648436	16
GTTATCA	60	4.077567E-4	12.648436	17
GTTGTAT	55	0.0030643085	12.073507	15
ATGGGAG	80	3.638894E-4	10.706562	5
GAAGCAG	945	0.0	10.339595	9
GGAAGCA	945	0.0	10.339595	8
GGGAAGC	1010	0.0	9.768099	7
AAGCAGT	1040	0.0	9.577541	10
>>END_MODULE