FastQCFastQC Report
Thu 26 May 2016
SRR1513159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93251
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC39294.213359642255847No Hit
CCCATGTACTCTGCGTTGATACCAC37704.042852087377079No Hit
GTATCAACGCAGAGTACTTTTTTTT16001.7157992943775402No Hit
GAGTACATGGGAAGCAGTGGTATCA15111.6203579586277894No Hit
TATCAACGCAGAGTACTTTTTTTTT12941.3876526793278356No Hit
CATGTACTCTGCGTTGATACCACTG11721.2568229831315483No Hit
TATCAACGCAGAGTACATGGGAAGC11421.2246517463619693No Hit
GGTATCAACGCAGAGTACTTTTTTT10561.1324275342891765No Hit
GTATCAACGCAGAGTACATGGGAAG9461.0144663328007206No Hit
ACGCAGAGTACATGGGAAGCAGTGG9290.9962359652979593No Hit
GCGTTGATACCACTGCTTCCCATGT9050.9704989758822962No Hit
GGTATCAACGCAGAGTACATGGGAA7640.8192941630652755No Hit
ACTCTGCGTTGATACCACTGCTTCC7200.7721096824698931No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7110.7624583114390194No Hit
CTTATACACATCTCCGAGCCCACGA6520.6991882124588477No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6150.659510353776367No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4040.43323932183032887No Hit
GGGAAGCAGTGGTATCAACGCAGAG4020.43109457271235696No Hit
ATACCACTGCTTCCCATGTACTCTG4010.430022198153371No Hit
GTACTCTGCGTTGATACCACTGCTT3960.4246603253584412No Hit
GCTTCCCATGTACTCTGCGTTGATA3760.40321283417872195No Hit
ATACACATCTCCGAGCCCACGAGAC3650.3914167140298764No Hit
TACCACTGCTTCCCATGTACTCTGC3630.3892719649119044No Hit
GCAGAGTACATGGGAAGCAGTGGTA3600.38605484123494654No Hit
GTGGTATCAACGCAGAGTACATGGG3560.38176534299900267No Hit
CATGGGAAGCAGTGGTATCAACGCA3550.3806929684400167No Hit
CAGTGGTATCAACGCAGAGTACATG3340.35817310270131153No Hit
CCACTGCTTCCCATGTACTCTGCGT3190.3420874843165221No Hit
CTGCTTCCCATGTACTCTGCGTTGA3180.34101510975753613No Hit
TCTCCGAGCCCACGAGACCTCTCTA3100.3324361132856484No Hit
GCAGTGGTATCAACGCAGAGTACAT3050.3270742404907186No Hit
AAGCAGTGGTATCAACGCAGAGTAC2980.31956761857781685No Hit
ACATGGGAAGCAGTGGTATCAACGC2820.30240962563404145No Hit
GATACCACTGCTTCCCATGTACTCT2820.30240962563404145No Hit
ACACATCTCCGAGCCCACGAGACCT2560.27452788710040643No Hit
GTTGATACCACTGCTTCCCATGTAC2520.2702383888644626No Hit
ATGGGAAGCAGTGGTATCAACGCAG2420.25951464327460294No Hit
CCATGTACTCTGCGTTGATACCACT2400.25736989415663103No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2380.2552251450386591No Hit
CTTCCCATGTACTCTGCGTTGATAC2140.22948815562299602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1870.20053404253037502No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1780.19088267149950133No Hit
ATCAACGCAGAGTACATGGGAAGCA1720.18444842414558557No Hit
TGATACCACTGCTTCCCATGTACTC1700.18230367502761363No Hit
TTCCCATGTACTCTGCGTTGATACC1660.1780141767916698No Hit
ATTCCATTCCATTCCATTCCATTCC1560.16729043120181017No Hit
ATCAACGCAGAGTACTTTTTTTTTT1530.16407330752485227No Hit
GTGGTATCAACGCAGAGTACTTTTT1380.14798768914006283No Hit
GGAAGCAGTGGTATCAACGCAGAGT1340.143698190904119No Hit
CATCTCCGAGCCCACGAGACCTCTC1290.13833631810918917No Hit
AAAAAGTACTCTGCGTTGATACCAC1230.1319020707552734No Hit
GTACTGGTTCACTATCGGTCAGTCA1200.12868494707831551No Hit
GTACATGGTAAGCAGTGGTATCAAC1160.12439544884237166No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT940.10080320854468049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGC250.005954454719.0198696
CTTATAC607.3696356E-817.4348811
CTCTCTA402.7381338E-416.61561619
TTATACA651.8353603E-716.0937372
CCTCTCT456.7070546E-414.76943718
TATACAC801.228891E-714.2649033
ACCTCTC500.001487513513.29249217
AGACCTC500.001487513513.29249215
GACCTCT500.001487513513.29249216
CCATGTA8900.010.2579092
TGCGTTG9800.09.59152212
CCCATGT9350.09.5607911
CTGCGTT9850.09.54283411
TACACAT1106.5753025E-59.5099355
ATACACA1106.5753025E-59.5099354
TGATACC10300.09.40245717
TCTGCGT10000.09.39969210
CTCTGCG10200.09.3084699
TACTCTG10050.09.263427
TGTACTC9800.09.2188155
Produced by FastQC (version 0.11.5)