FastQCFastQC Report
Thu 26 May 2016
SRR1513158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907068
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC20530.10765216552320106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT1850.011.8674771
TGGCGAG8500.011.27752718
TAAAGCG600.00583087111.0949425
TAGGACC3950.010.8500924
CGTGAAA5700.010.822499
TTAGGAC6700.010.661443
GTATTAT2150.010.655531
ACGTGAA5750.010.5627758
GGCGAGG4150.010.52013219
GCGCCAC1002.4258141E-510.4395413
TATGGCG9300.010.40947116
TAGGACT2750.010.0434584
GACGTGA5600.09.9983857
GTCCTAA6250.09.92740251
GTAGGAC10150.09.852643
TAATACC1451.5588193E-79.8523824
GTCTAGA2159.094947E-129.767571
GGACGTG8550.09.7667096
CTTACAC2054.0017767E-119.7565173
GTCCTAG2350.09.7486761
Produced by FastQC (version 0.11.5)