##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513158_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1907068 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.916428255311295 31.0 30.0 34.0 22.0 34.0 2 28.751234355565717 31.0 30.0 34.0 16.0 34.0 3 28.919567629471 31.0 30.0 34.0 16.0 34.0 4 31.97540779877802 35.0 32.0 37.0 18.0 37.0 5 32.092813156111895 35.0 33.0 37.0 19.0 37.0 6 32.45668062177122 36.0 33.0 37.0 23.0 37.0 7 32.39258642062055 36.0 33.0 37.0 23.0 37.0 8 32.27641017520089 36.0 33.0 37.0 22.0 37.0 9 33.42744936205736 38.0 33.0 39.0 17.0 39.0 10 32.98600364538653 38.0 33.0 39.0 17.0 39.0 11 33.457561030859935 38.0 33.0 39.0 18.0 39.0 12 33.43666822577905 38.0 34.0 39.0 18.0 39.0 13 33.486674308414806 38.0 34.0 39.0 18.0 39.0 14 33.66315044875169 39.0 33.0 41.0 10.0 41.0 15 34.07218148487626 39.0 33.0 41.0 17.0 41.0 16 33.9073231788274 39.0 33.0 41.0 11.0 41.0 17 33.51403777946041 39.0 32.0 41.0 10.0 41.0 18 33.233948658359324 38.0 32.0 41.0 10.0 41.0 19 33.86860982408598 39.0 33.0 41.0 11.0 41.0 20 34.07716295381182 39.0 34.0 41.0 15.0 41.0 21 34.12424255453922 39.0 34.0 41.0 10.0 41.0 22 33.80092634347595 39.0 34.0 41.0 8.0 41.0 23 33.31519851415891 39.0 33.0 41.0 2.0 41.0 24 33.55248370797476 39.0 33.0 41.0 2.0 41.0 25 33.162368095946235 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3047775961761303 1101 2 1.474019539378311 1101 3 1.36462045876981 1101 4 1.9166196812518876 1101 5 1.7833669388744724 1101 6 1.4531899940552435 1101 7 1.3845191837756303 1101 8 1.4286915323254092 1101 9 1.8732992566831044 1101 10 2.2889057954523295 1101 11 1.8494543280969538 1101 12 1.701638444044974 1101 13 1.644635747562738 1101 14 2.687943557630348 1101 15 2.300596868465881 1101 16 2.386172145930196 1101 17 2.7201753290022808 1101 18 2.8060458905055157 1101 19 2.219859614011945 1101 20 2.01469578121651 1101 21 2.009531533078878 1101 22 2.2000360326795843 1101 23 2.4554495481858183 1101 24 2.0924407761674857 1101 25 2.3748710333955287 1102 1 0.8999642265061958 1102 2 1.110904013392478 1102 3 1.1166642720675988 1102 4 1.382606339176931 1102 5 1.303479431824968 1102 6 0.9671377594651318 1102 7 0.9186487914011039 1102 8 0.9443247863955477 1102 9 1.3857909287409242 1102 10 1.6554605625569891 1102 11 1.2378617119725135 1102 12 1.1032828538188042 1102 13 1.1123394932914294 1102 14 2.196126047266553 1102 15 1.5975511452290334 1102 16 1.7428369841884148 1102 17 2.1551867457004263 1102 18 2.2644667245202825 1102 19 1.6626831349670042 1102 20 1.454437420830935 1102 21 1.3835164407490765 1102 22 1.5021706392413066 1102 23 1.8100673167459718 1102 24 1.6572664188482307 1102 25 2.0058875947236814 1103 1 0.8526063370202195 1103 2 1.1929548375994479 1103 3 0.96629468269726 1103 4 1.461505450348504 1103 5 1.2095019813113979 1103 6 0.9828013764526418 1103 7 0.9230830135628665 1103 8 0.9138676746344387 1103 9 1.3547839677632112 1103 10 1.597685297835774 1103 11 1.2135762325266697 1103 12 1.121923875971497 1103 13 1.1032088785940104 1103 14 1.883182483336661 1103 15 1.5391665115306452 1103 16 1.6158983405587293 1103 17 1.9201375426983631 1103 18 2.142438461136841 1103 19 1.65983752355735 1103 20 1.3017408922501161 1103 21 1.3686903518208808 1103 22 1.639396281487862 1103 23 1.950515528885738 1103 24 1.6558563673778082 1103 25 1.798230235813854 1104 1 0.5481412063770037 1104 2 0.8160951338487301 1104 3 0.6323795795370053 1104 4 1.0637813650776735 1104 5 1.0243942263951595 1104 6 0.6621185855063416 1104 7 0.5476477486781519 1104 8 0.6137934423156182 1104 9 0.9689822681642184 1104 10 1.1419301445450856 1104 11 0.8691450022534823 1104 12 0.7304728230247761 1104 13 0.7917021844632757 1104 14 1.648306609973595 1104 15 1.3111130935384665 1104 16 1.446377365678721 1104 17 1.8307232508047946 1104 18 1.9670217543318316 1104 19 1.2985154894029236 1104 20 1.0757542446231838 1104 21 1.035258089284028 1104 22 1.3053814227464855 1104 23 1.567366855528718 1104 24 1.3357227633703985 1104 25 1.6784783238122074 1105 1 0.6594988062999434 1105 2 1.0403160037148318 1105 3 0.9071801182474353 1105 4 1.3581250349066565 1105 5 1.1757167685052892 1105 6 0.7811009774469966 1105 7 0.8293725817777684 1105 8 0.8573300991236437 1105 9 1.184058915377456 1105 10 1.5243804443206699 1105 11 1.1083007261929652 1105 12 1.0075520960456075 1105 13 0.943446836376765 1105 14 1.7223061397604482 1105 15 1.4004540624057213 1105 16 1.7425270868145688 1105 17 2.1144838316297623 1105 18 1.5965898815486028 1105 19 1.4397369168679646 1105 20 1.3350328096897854 1105 21 1.3113707085088748 1105 22 1.3246862717637242 1105 23 1.7953601332919078 1105 24 1.6363026968419447 1105 25 1.8414895086046812 1106 1 0.6062956948927933 1106 2 0.7899811998773068 1106 3 0.7296424781097635 1106 4 1.173091478424567 1106 5 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1.5579972630661558 1107 18 1.6537685100567785 1107 19 1.1175736698333196 1107 20 0.929415883985989 1107 21 0.7179995354359363 1107 22 1.0527449473161141 1107 23 1.3038580912637414 1107 24 1.0536407733856024 1107 25 1.3590250093767722 1108 1 0.7245152709483342 1108 2 0.9367830828037285 1108 3 0.7552553982084902 1108 4 1.1671284509794475 1108 5 1.1567728310178822 1108 6 0.792784984854876 1108 7 0.692983308002816 1108 8 0.723223338175913 1108 9 0.943548574457111 1108 10 1.1483555822765723 1108 11 0.8155724191971743 1108 12 0.6421892224550021 1108 13 0.5663363161177628 1108 14 1.6080388608396774 1108 15 1.230376989977124 1108 16 1.2262814999215905 1108 17 1.5866174171001646 1108 18 1.9364515615010163 1108 19 1.284361087978084 1108 20 1.0195522034672422 1108 21 0.9538072053720583 1108 22 1.184215125276026 1108 23 1.5675722454743664 1108 24 1.353483275426207 1108 25 1.7399688059721043 1109 1 0.4637702272592712 1109 2 0.6691793747387678 1109 3 0.6046336867182944 1109 4 0.9094225772982654 1109 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10 -0.3707791938379117 2211 11 -0.4549681744799585 2211 12 -0.4605104098662096 2211 13 -0.5697875684134814 2211 14 -0.07600670001503573 2211 15 -0.19684965512522012 2211 16 -6.9148393643593025 2211 17 -2.1629609043523317 2211 18 -0.830459171641408 2211 19 -0.8403892631983041 2211 20 -0.953461703918272 2211 21 -0.8759180630476919 2211 22 -0.7056071203134735 2211 23 -0.2627490664618932 2211 24 -0.8165525939388516 2211 25 -7.922334733191956 2212 1 -1.5969689332860675 2212 2 -4.314059865285735 2212 3 -1.9973700904753962 2212 4 -3.817699452006778 2212 5 -1.5892229368607431 2212 6 -1.3519617669564497 2212 7 -1.2337178220480638 2212 8 -1.17160084755184 2212 9 -1.1777176740197746 2212 10 -0.5876562147994235 2212 11 -1.1873448861154472 2212 12 -1.3118136321067766 2212 13 -1.5484558327396023 2212 14 -6.828802184065108 2212 15 -5.458551649637574 2212 16 -3.135751063434853 2212 17 -7.893540774095207 2212 18 -3.6470835448519665 2212 19 -2.5013476782730883 2212 20 -2.497413088703464 2212 21 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-0.5457558098580968 2214 8 -0.2622350801399378 2214 9 -0.17643450392340299 2214 10 -3.684383638101256 2214 11 -1.2977550753125797 2214 12 -0.6476326406908228 2214 13 -0.3711200324532484 2214 14 0.4544627185090704 2214 15 0.2109305222649951 2214 16 0.21918125419740164 2214 17 0.3432769560988831 2214 18 0.8358305747693606 2214 19 0.04039866992042107 2214 20 -0.2996714481558058 2214 21 -0.30502649383697644 2214 22 0.12911164963613686 2214 23 0.21777672031669226 2214 24 -0.09586408627140486 2214 25 0.024862474202471674 2215 1 -2.4825711702433715 2215 2 -4.8310163307249425 2215 3 -3.1087959796147864 2215 4 -2.141368438905623 2215 5 -2.1543795143869993 2215 6 -2.2845097348941117 2215 7 -2.4869797077910256 2215 8 -2.6454021491307493 2215 9 -8.40317770310962 2215 10 -4.209578202542431 2215 11 -3.4576390135870447 2215 12 -3.8197067378459373 2215 13 -3.2116407421441515 2215 14 -8.329852742417799 2215 15 -5.299621140721953 2215 16 -9.700091628266158 2215 17 -5.194288722155768 2215 18 -3.562719482154151 2215 19 -4.031495300794585 2215 20 -3.699212564228443 2215 21 -3.928993959534349 2215 22 -9.900235785047318 2215 23 -10.959818472485683 2215 24 -7.350223574513478 2215 25 -5.696248287998699 2216 1 -2.1729491922131743 2216 2 -2.2560110962582236 2216 3 -2.0540668258785004 2216 4 -6.891699799343524 2216 5 -3.7370574488664303 2216 6 -3.1545608270282024 2216 7 -2.597989193392504 2216 8 -8.255035717765825 2216 9 -4.535594755506629 2216 10 -2.676983832585279 2216 11 -2.6745361525770903 2216 12 -2.836063978982864 2216 13 -2.5358313627275706 2216 14 -1.8991187580165096 2216 15 -2.4521254591569956 2216 16 -2.1290734684055437 2216 17 -1.9891635249285393 2216 18 -7.768037232978578 2216 19 -5.209575597514068 2216 20 -4.730254353967393 2216 21 -3.77962845167891 2216 22 -3.5037581137867804 2216 23 -2.6832773893997413 2216 24 -3.0497427513561988 2216 25 -2.3585695683492602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 106469.0 3 3.0 4 859.0 5 5148.0 6 6239.0 7 5398.0 8 4908.0 9 4823.0 10 4408.0 11 4323.0 12 3951.0 13 4139.0 14 4077.0 15 4068.0 16 4264.0 17 4480.0 18 5196.0 19 6389.0 20 8094.0 21 10253.0 22 12618.0 23 15197.0 24 17639.0 25 19912.0 26 22631.0 27 25146.0 28 28283.0 29 33396.0 30 40035.0 31 47711.0 32 60119.0 33 77684.0 34 100361.0 35 131782.0 36 183850.0 37 262061.0 38 631154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70186416311729 18.417507197231725 12.077115035425456 25.803513604225525 2 14.822598109815713 20.24258065872607 40.50970848270134 24.425112748756874 3 18.47307057475373 24.879834733334032 29.59566437830507 27.051430313607167 4 11.752125502022833 16.612980673042298 38.74368456854994 32.891209256384926 5 12.959298080728916 37.85809914735857 36.06256383246576 13.120038939446744 6 32.12458738518373 36.99399289468633 18.064478571301205 12.816941148828734 7 28.061703188844213 31.480940515780592 22.468809345369927 17.988546950005272 8 25.53405064539205 35.78018699901613 20.854302718555363 17.831459637036463 9 26.82764375257968 14.720639670724397 19.81391187325323 38.6378047034427 10 14.208385102777536 28.58664565994175 35.3428531419092 21.86211609537151 11 34.709048598804316 22.280041936483745 24.276797575697817 18.73411188901412 12 23.18279136757059 24.629467034655807 31.461288234732592 20.72645336304101 13 28.943974345592853 21.48435963375124 25.63075214009639 23.940913880559517 14 21.10722233556957 21.04480844968628 28.408125973250144 29.439843241494014 15 23.660541459243678 29.519967686602005 23.30315317914527 23.51633767500905 16 22.90582108260913 27.47069528869402 27.01529876382632 22.608184864870534 17 22.217081419302247 27.648759361638177 27.755247422123393 22.378911796936187 18 22.256811420275017 26.95918812653947 28.841901197165072 21.942099256020445 19 23.72977721831032 26.765692406540527 27.20779408100914 22.296736294140015 20 23.983727470802915 26.809285580159937 27.242114715456278 21.964872233580866 21 23.6842974885822 26.475869061550426 26.768034669619446 23.071798780247928 22 23.630786792350882 27.108855617206267 26.712949289682044 22.54740830076081 23 22.910532074367257 26.882810623234366 27.256682006862704 22.949975295535676 24 23.330196172243785 26.766646626607532 27.23811262513233 22.665044576016353 25 23.264627631672816 26.565312905215716 27.297166584836695 22.872892878274772 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 292.0 3 399.0 4 399.0 5 399.0 6 802.5 7 1206.0 8 1206.0 9 1206.0 10 1944.5 11 2683.0 12 2683.0 13 2683.0 14 5011.5 15 7340.0 16 7340.0 17 7340.0 18 13763.5 19 20187.0 20 20187.0 21 20187.0 22 32260.0 23 44333.0 24 44333.0 25 44333.0 26 62789.0 27 81245.0 28 81245.0 29 81245.0 30 104712.0 31 128179.0 32 128179.0 33 128179.0 34 149578.0 35 170977.0 36 170977.0 37 170977.0 38 189239.0 39 207501.0 40 207501.0 41 207501.0 42 220951.0 43 234401.0 44 234401.0 45 234401.0 46 243929.5 47 253458.0 48 253458.0 49 253458.0 50 246290.5 51 239123.0 52 239123.0 53 239123.0 54 216372.5 55 193622.0 56 193622.0 57 193622.0 58 167670.0 59 141718.0 60 141718.0 61 141718.0 62 116324.0 63 90930.0 64 90930.0 65 90930.0 66 70561.5 67 50193.0 68 50193.0 69 50193.0 70 36915.5 71 23638.0 72 23638.0 73 23638.0 74 16682.5 75 9727.0 76 9727.0 77 9727.0 78 6834.5 79 3942.0 80 3942.0 81 3942.0 82 2700.0 83 1458.0 84 1458.0 85 1458.0 86 954.0 87 450.0 88 450.0 89 450.0 90 289.0 91 128.0 92 128.0 93 128.0 94 80.5 95 33.0 96 33.0 97 33.0 98 22.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16837365002191845 2 0.2416274616322019 3 0.2458223828410943 4 0.40648786514167295 5 0.24262375541931383 6 0.017094303926236506 7 0.01714674044134766 8 0.016779684835569575 9 0.01767110559245921 10 0.019768566196905406 11 0.019663693166683094 12 0.02569389240446591 13 0.025956074980021684 14 0.023124503164019324 15 0.03869814815203233 16 0.01982100271201656 17 0.039379822848477346 18 0.02957419452269138 19 0.02585120194979938 20 0.028420591190245967 21 0.03224845679336028 22 0.04242114072492434 23 0.027791353008912112 24 0.046458752388483265 25 0.03544708421514073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1907068.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.78842525654427 #Duplication Level Percentage of deduplicated Percentage of total 1 83.96873514484973 62.79889472267156 2 10.99805248590688 16.45054052619595 3 2.7308606503421573 6.12710302902457 4 0.9739180554882038 2.913511907955139 5 0.44201955803180165 1.6528973338896067 6 0.23025702781602772 1.0332336308767816 7 0.14468330829861953 0.75744457519926 8 0.1022142736464151 0.6115555651805502 9 0.07834125160299464 0.5273116956013225 >10 0.3060411138523631 3.935616001271918 >50 0.01487518782633135 0.7722704386540028 >100 0.008371252722961079 1.2615620899700353 >500 9.925936790223064E-4 0.46354154133877956 >1k 6.380959365143268E-4 0.6945169421705824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2053 0.10765216552320106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 5.243651511115493E-5 6 0.0 0.0 0.0 0.0 5.243651511115493E-5 7 0.0 0.0 0.0 0.0 5.243651511115493E-5 8 0.0 0.0 0.0 0.0 5.243651511115493E-5 9 0.0 0.0 0.0 5.243651511115493E-5 5.243651511115493E-5 10 0.0 0.0 0.0 5.243651511115493E-5 5.243651511115493E-5 11 0.0 0.0 0.0 5.243651511115493E-5 5.243651511115493E-5 12 0.0 0.0 0.0 5.243651511115493E-5 5.243651511115493E-5 13 0.0 0.0 0.0 5.243651511115493E-5 1.5730954533346478E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAT 185 0.0 11.867477 1 TGGCGAG 850 0.0 11.277527 18 TAAAGCG 60 0.005830871 11.094942 5 TAGGACC 395 0.0 10.850092 4 CGTGAAA 570 0.0 10.82249 9 TTAGGAC 670 0.0 10.66144 3 GTATTAT 215 0.0 10.65553 1 ACGTGAA 575 0.0 10.562775 8 GGCGAGG 415 0.0 10.520132 19 GCGCCAC 100 2.4258141E-5 10.43954 13 TATGGCG 930 0.0 10.409471 16 TAGGACT 275 0.0 10.043458 4 GACGTGA 560 0.0 9.998385 7 GTCCTAA 625 0.0 9.9274025 1 GTAGGAC 1015 0.0 9.85264 3 TAATACC 145 1.5588193E-7 9.852382 4 GTCTAGA 215 9.094947E-12 9.76757 1 GGACGTG 855 0.0 9.766709 6 CTTACAC 205 4.0017767E-11 9.756517 3 GTCCTAG 235 0.0 9.748676 1 >>END_MODULE