FastQCFastQC Report
Thu 26 May 2016
SRR1513158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907068
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25480.13360824050322276No Hit
GTACATGGGAAGCAGTGGTATCAAC22690.11897845278721053No Hit
CCCATGTACTCTGCGTTGATACCAC22580.11840165112098783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA508.795817E-515.1836811
TAACCCG400.005204686414.2807615
ATCGTCC400.00531929614.23395258
TAGGACC4700.012.5589664
GCCTAAT1052.6350062E-711.7910171
TAGACTG2200.010.8187585
TGTAGGA12350.010.7167972
AATGCCG803.806375E-410.67686418
GTAGGAC11250.010.6638093
TGGCGAG10350.010.54505118
GGCGAGG4750.010.18771319
TAGACAG3100.010.1347335
CCGACAT750.002668839910.1237814
TATGGCG11350.010.11820616
GTGTAGA2650.010.062581
GTCCTAC10900.010.0477431
TCGTCCG856.666276E-410.04696910
TGTACCG1356.2557956E-79.8731185
CTGTAGG12950.09.7809061
GGCGAGA6500.09.63453319
Produced by FastQC (version 0.11.5)