FastQCFastQC Report
Thu 26 May 2016
SRR1513157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC50790.18284576263315178No Hit
GTCCTACAGTGGACATTTCTAAATT48480.17452968246613895No Hit
CCCATGTACTCTGCGTTGATACCAC42230.15202946556404803No Hit
GTCCTACAGTGTGCATTTCTCATTT40540.14594540691372265No Hit
CTGTAGGACGTGGAATATGGCAAGA38370.13813333160531668No Hit
GTCCTAAAGTGTGTATTTCTCATTT37270.13417329343054868No Hit
GTATCAACGCAGAGTACTTTTTTTT31840.11462510498601207No Hit
CTTTAGGACGTGAAATATGGCGAGG31410.1130770900631482No Hit
CTGTAGGACCTGGAATATGGCGAGA30820.11095306958759082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC400.005322478214.23299611
TAGGACC10950.014.1877874
GTAGGAC28650.012.1758023
TAGGACT6900.012.1555534
TGTAGGA31050.011.8878932
GGACCTG17550.011.7839416
AGGACGT22000.011.6725045
GGACGTG21550.011.4895876
CTGTAGG31100.011.4883881
AGGACCT17900.011.4768765
GGACCGC752.102285E-411.3839376
GTCCTAC27300.011.3378381
GGCGAGG10800.011.24663419
AGGACCG1104.8995935E-711.240195
GACGGTG600.005938049411.0681137
GTCCTAA13600.011.0283021
GACGTGG12700.010.9809647
TTAGGAC14700.010.8926393
TAGGACG23200.010.7635244
TGGCGAG26650.010.68199918
Produced by FastQC (version 0.11.5)