##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513157_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2777751
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.449113329452494	31.0	30.0	34.0	25.0	34.0
2	29.284805225522373	31.0	30.0	34.0	22.0	34.0
3	29.46656629769911	31.0	30.0	34.0	23.0	34.0
4	32.52102834271322	37.0	33.0	37.0	22.0	37.0
5	32.66973353623129	37.0	33.0	37.0	25.0	37.0
6	33.02915236102876	37.0	35.0	37.0	26.0	37.0
7	32.981950506002875	37.0	35.0	37.0	26.0	37.0
8	32.86339020308156	37.0	35.0	37.0	25.0	37.0
9	34.09981096217768	39.0	35.0	39.0	24.0	39.0
10	33.67306176831545	38.0	34.0	39.0	18.0	39.0
11	34.14502235801553	39.0	35.0	39.0	25.0	39.0
12	34.13276927989586	39.0	35.0	39.0	25.0	39.0
13	34.18152742992442	39.0	35.0	39.0	25.0	39.0
14	34.41015627390648	39.0	34.0	41.0	17.0	41.0
15	34.85812407231606	40.0	34.0	41.0	21.0	41.0
16	34.70508263699662	40.0	34.0	41.0	18.0	41.0
17	34.337819696581874	39.0	34.0	41.0	17.0	41.0
18	34.07917448324202	39.0	33.0	41.0	16.0	41.0
19	34.70551968120973	40.0	34.0	41.0	18.0	41.0
20	34.92123484070387	40.0	35.0	41.0	22.0	41.0
21	34.96084890258342	40.0	35.0	41.0	22.0	41.0
22	34.65498959409969	40.0	35.0	41.0	18.0	41.0
23	34.20597634561197	39.0	34.0	41.0	10.0	41.0
24	34.46145064838425	40.0	34.0	41.0	16.0	41.0
25	34.10714639289123	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0346229581090718
1101	2	1.2233414811340921
1101	3	1.1764262834649344
1101	4	1.5457223116454273
1101	5	1.380494533340368
1101	6	1.1137798764290352
1101	7	1.061323344912509
1101	8	1.1808845792685077
1101	9	1.4781510733103502
1101	10	1.8467292045824166
1101	11	1.462790405682476
1101	12	1.2737015966107066
1101	13	1.1813541278167463
1101	14	2.210022978802968
1101	15	1.777198741091361
1101	16	1.8576512053803498
1101	17	2.221191410197598
1101	18	2.2697741930917132
1101	19	1.8591539779033894
1101	20	1.4384238840153358
1101	21	1.4002744903111193
1101	22	1.7375056488187113
1101	23	1.9750080503962835
1101	24	1.5783460688757316
1101	25	1.909629463786061
1102	1	1.02036095057057
1102	2	1.2630524053483683
1102	3	1.1506703722014677
1102	4	1.6201984556975475
1102	5	1.5611604887190325
1102	6	1.1728893956098503
1102	7	1.2028755296786784
1102	8	1.258537614033429
1102	9	1.6134016609377682
1102	10	1.7841435288875687
1102	11	1.4129589052098765
1102	12	1.3090657075294558
1102	13	1.3179287943645406
1102	14	2.3116557418597026
1102	15	1.8944842139445086
1102	16	1.9048781048164969
1102	17	2.3562757989596292
1102	18	2.54908103238472
1102	19	1.9195884147876043
1102	20	1.6649224417878372
1102	21	1.6147520291395807
1102	22	1.7210354251368472
1102	23	2.0658031376201293
1102	24	1.8418941522325483
1102	25	2.0601520136289437
1103	1	0.7994544079326786
1103	2	1.0968499992664036
1103	3	0.9295612377773743
1103	4	1.3146683295398773
1103	5	1.1917146252102313
1103	6	0.8473577583987222
1103	7	0.8678597960110253
1103	8	0.8427263577913635
1103	9	1.2157902433989847
1103	10	1.516045280285617
1103	11	1.0506112271071046
1103	12	1.0445367931566452
1103	13	0.9905839518406765
1103	14	1.9173865297975894
1103	15	1.498214209403109
1103	16	1.4799780502938304
1103	17	1.8794555826647965
1103	18	2.176960992603057
1103	19	1.556980774294054
1103	20	1.2953932585087031
1103	21	1.3868626895629177
1103	22	1.4419320235366087
1103	23	1.8801822235104524
1103	24	1.5050717446748365
1103	25	1.7733739550196077
1104	1	0.761143144831415
1104	2	0.8941634129391893
1104	3	0.8092207756182894
1104	4	1.1251353520047118
1104	5	0.9903057627326675
1104	6	0.7579877742310686
1104	7	0.6404213585688581
1104	8	0.7117736554167138
1104	9	1.1077348298256169
1104	10	1.2179066446117162
1104	11	0.8845541729393318
1104	12	0.8053053232232159
1104	13	0.8364677899231125
1104	14	1.7435541496923292
1104	15	1.28045201123372
1104	16	1.4676260244818735
1104	17	1.8277087227437292
1104	18	1.939872330398991
1104	19	1.341247128585735
1104	20	1.1459925598365786
1104	21	1.0404920498759438
1104	22	1.3185674469108974
1104	23	1.495951721733995
1104	24	1.3155845967122914
1104	25	1.6336819413353965
1105	1	0.7543412156846863
1105	2	0.9886990226574461
1105	3	0.8903510496603317
1105	4	1.192178312264197
1105	5	1.0290215691162459
1105	6	0.8128024041733397
1105	7	0.8785366776474461
1105	8	0.8238085046840311
1105	9	1.0839245597633393
1105	10	1.4860733103382984
1105	11	0.9386697869525165
1105	12	0.8442230830484192
1105	13	0.941129506859987
1105	14	1.567140113619942
1105	15	1.2789658827835382
1105	16	1.4701352945402633
1105	17	1.7416958041199138
1105	18	1.4084668257401347
1105	19	1.3063055197628657
1105	20	1.118508632812656
1105	21	1.0115966584441765
1105	22	1.1778209006898166
1105	23	1.4821959186254006
1105	24	1.290703444248372
1105	25	1.5237824604191204
1106	1	0.4625380438166431
1106	2	0.6607643569126793
1106	3	0.5647042412575232
1106	4	1.024503065730272
1106	5	0.8299208643773497
1106	6	0.49794850646968314
1106	7	0.4887912500437679
1106	8	0.43313715450630497
1106	9	0.6641614624035057
1106	10	0.4862052402272141
1106	11	0.4926449994179549
1106	12	0.47590620676204765
1106	13	0.3923490337706639
1106	14	1.4071200184454042
1106	15	0.9470612536131142
1106	16	1.0609032592280982
1106	17	1.3754934260836649
1106	18	1.5721536064859336
1106	19	0.9072031256778317
1106	20	0.7949196088967412
1106	21	0.6484186122212492
1106	22	0.8947979042643937
1106	23	1.4462486439228357
1106	24	0.9111644828986059
1106	25	1.2208738488162894
1107	1	0.6265988886534508
1107	2	0.7692084475287508
1107	3	0.7082206187080757
1107	4	1.0400338834937273
1107	5	0.9480919921722943
1107	6	0.6884513123543954
1107	7	0.6169895652548618
1107	8	0.6301676689044342
1107	9	0.9693665025343421
1107	10	1.178398655742889
1107	11	0.7919678665214605
1107	12	0.7635588882057505
1107	13	0.7237783030149885
1107	14	1.5630875984175745
1107	15	0.8923944346347383
1107	16	1.0658735190252386
1107	17	1.5715706503560014
1107	18	1.7538572800329604
1107	19	1.141008362505481
1107	20	0.9898820613210049
1107	21	0.8177959408248725
1107	22	1.133067037150596
1107	23	1.3347783366261368
1107	24	1.122477295192823
1107	25	1.397880405991863
1108	1	0.5750406686793426
1108	2	0.7488537395449733
1108	3	0.6397107024580144
1108	4	1.0456384265344738
1108	5	0.9483967344856836
1108	6	0.6852928655724426
1108	7	0.5623075968810127
1108	8	0.5969696424992392
1108	9	0.8617446368989903
1108	10	1.1546248440479374
1108	11	0.7672616685075724
1108	12	0.5417406236925473
1108	13	0.5761025470484284
1108	14	1.5688965322508324
1108	15	1.1178580407811722
1108	16	1.1263799544855786
1108	17	1.4312956514288047
1108	18	1.650258415234397
1108	19	1.000019448554788
1108	20	0.9660276778592873
1108	21	0.8984327460973276
1108	22	1.0887437930563593
1108	23	1.3422906201886917
1108	24	1.1092586643183466
1108	25	1.396032619357527
1109	1	0.32121827417353543
1109	2	0.48080201906918774
1109	3	0.3886770009570242
1109	4	0.6502250344359197
1109	5	0.6940238585298601
1109	6	0.36650082719804544
1109	7	0.3075178772548455
1109	8	0.3064765102420921
1109	9	0.41968236700378014
1109	10	0.871318946285264
1109	11	0.5137406227698236
1109	12	0.4271515377395616
1109	13	0.4285885637575788
1109	14	1.1935137617892053
1109	15	0.8035481781360261
1109	16	0.9322303686741904
1109	17	1.1897307618791046
1109	18	1.3651417698081687
1109	19	0.8536449264647246
1109	20	0.7584607704676145
1109	21	0.6114655271791705
1109	22	0.785718357627303
1109	23	1.1259992013271827
1109	24	0.8227096165981536
1109	25	1.0932545465736112
1110	1	0.4142288961702292
1110	2	0.5886643368088329
1110	3	0.48762728115722354
1110	4	0.8821776239545684
1110	5	0.8454477573712964
1110	6	0.41063767602015844
1110	7	0.47588748540864856
1110	8	0.3649699838129834
1110	9	0.6536538738340667
1110	10	0.9423419917206672
1110	11	0.47680422452525306
1110	12	0.462838321232276
1110	13	0.26004228406091556
1110	14	1.0410602194596592
1110	15	0.7683864173241233
1110	16	0.9381096894220278
1110	17	1.2470858543169498
1110	18	0.9632165057775453
1110	19	0.7638970849395363
1110	20	0.4757247707107055
1110	21	0.33653400368012143
1110	22	0.6286059172593781
1110	23	-0.5858065381105106
1110	24	0.08640328897879357
1110	25	0.5680642014346802
1111	1	0.1572415669006091
1111	2	0.47791741810226185
1111	3	0.311398066587504
1111	4	0.68239583777153
1111	5	0.5989463589100765
1111	6	0.27689612238420125
1111	7	0.2507816972706536
1111	8	0.3318335375811614
1111	9	0.5179294147138123
1111	10	0.7284258627728875
1111	11	0.40174997713244665
1111	12	0.318522904007736
1111	13	0.17520695203110392
1111	14	1.0333593944564328
1111	15	0.5870092494643799
1111	16	0.7031559621846171
1111	17	1.0781155652704086
1111	18	1.3175802338676732
1111	19	0.7779322105721675
1111	20	0.3573757650803415
1111	21	0.1765321130188937
1111	22	0.513431540488277
1111	23	0.843387134263665
1111	24	0.6161438372428663
1111	25	0.9436664105701311
1112	1	0.10900752823430082
1112	2	0.29924056443594793
1112	3	0.24652608879369708
1112	4	0.6787706598454477
1112	5	0.3099207616577715
1112	6	0.1620318304052688
1112	7	0.06253822418728561
1112	8	0.19368984265578604
1112	9	0.36703683470733495
1112	10	0.5944084038153505
1112	11	0.2044987184348912
1112	12	0.24333597061192336
1112	13	0.14397162062552837
1112	14	0.6481685536642274
1112	15	0.5264160610204627
1112	16	0.5309185950424364
1112	17	0.862154633314077
1112	18	1.1051480635627016
1112	19	0.5602210377865902
1112	20	0.44739928134805496
1112	21	0.3206100076426708
1112	22	0.5846160884270404
1112	23	0.9178315460524971
1112	24	0.6798629428043128
1112	25	1.0418397450424166
1113	1	-0.016353214179094522
1113	2	0.10324426506423023
1113	3	0.09175032949764272
1113	4	0.35010217640400754
1113	5	0.37995822336993257
1113	6	0.10161515491245154
1113	7	0.031036069900530094
1113	8	0.06473375028200934
1113	9	0.19720685813546623
1113	10	0.4750449245892838
1113	11	0.131177611084901
1113	12	0.01791411593413983
1113	13	-0.13084816703847935
1113	14	0.4372842157737793
1113	15	0.20490390847069762
1113	16	0.3457756008168573
1113	17	0.6468254108484857
1113	18	0.6517993338928534
1113	19	0.3090801872563844
1113	20	0.19123838133491944
1113	21	0.028058872458530004
1113	22	0.16961429848377207
1113	23	0.2734277666243443
1113	24	0.131177968583728
1113	25	0.4834583621470756
1114	1	-1.1643033928153521
1114	2	-0.7372094023881104
1114	3	-0.7890687377728938
1114	4	-0.4931838329149869
1114	5	-3.614142982702109
1114	6	-1.4450705154817953
1114	7	-1.1379509846147826
1114	8	-0.867759987857518
1114	9	-0.7645528988701571
1114	10	-0.3399685595837454
1114	11	-0.781463947937624
1114	12	-1.0213996410542592
1114	13	-0.8791428556601701
1114	14	-6.3277264627308085
1114	15	-2.6675237307302595
1114	16	-1.5810537145817705
1114	17	-0.5808503812439128
1114	18	-6.796049866778734
1114	19	-2.8009885607551794
1114	20	-1.6994832078792044
1114	21	-1.424353467367169
1114	22	-1.1695740516500805
1114	23	-0.6787136369771929
1114	24	-0.9083802338988818
1114	25	-0.7032016892318964
1115	1	-0.9407952785655027
1115	2	-0.5873950222864153
1115	3	-0.6388038620734982
1115	4	-3.2669492770754722
1115	5	-1.2986763724920856
1115	6	-0.9847495302379627
1115	7	-0.7908593632774696
1115	8	-0.5882764320476781
1115	9	-0.4640645292173815
1115	10	-0.7041815155529108
1115	11	-0.7232313748191572
1115	12	-0.8125790943718698
1115	13	-0.9730263784046471
1115	14	-6.352747457587014
1115	15	-2.683396407454211
1115	16	-1.4736417134651134
1115	17	-0.5881745499799536
1115	18	-0.4280270881040735
1115	19	-0.6989250127042936
1115	20	-0.7120966342692583
1115	21	-0.8893887191605785
1115	22	-1.9806710347006273
1115	23	-1.20407191558062
1115	24	-0.8577515941378877
1115	25	-0.4725483935561172
1116	1	-0.562287325807528
1116	2	-0.2870125878275296
1116	3	-0.31726353548920727
1116	4	-0.2074815967780097
1116	5	-0.8930597920502557
1116	6	-0.48445544994874723
1116	7	-0.4980818079311433
1116	8	-0.3683474424490143
1116	9	-0.358441807003949
1116	10	-0.17750109292840932
1116	11	-0.600848817153171
1116	12	-0.8290940487494751
1116	13	-0.8424622562185178
1116	14	0.03381518239729786
1116	15	-0.3659829146788738
1116	16	-0.4439841111923073
1116	17	0.030435463969574528
1116	18	-0.16008881764739868
1116	19	-0.3728708556587321
1116	20	-0.8065880690995328
1116	21	-0.8461736438895002
1116	22	-0.7391134851392707
1116	23	-1.4488803763959837
1116	24	-1.191504847488197
1116	25	-0.6059782422130127
1201	1	0.9460073132806954
1201	2	1.154207336191046
1201	3	1.135775721581286
1201	4	1.5266994922891115
1201	5	1.3256611872659079
1201	6	1.128482498552728
1201	7	1.021409538628589
1201	8	1.108794811666641
1201	9	1.3280187248660411
1201	10	1.7682566107440252
1201	11	1.2470089287311694
1201	12	1.2102474455519214
1201	13	1.01648666737794
1201	14	1.9921731393411548
1201	15	1.7244877480480127
1201	16	1.7889833856417994
1201	17	2.1383924078277445
1201	18	1.4496304731926202
1201	19	1.4997863836827676
1201	20	1.5287538473208997
1201	21	1.4810213751058612
1201	22	1.6209329479548629
1201	23	1.9939013880615946
1201	24	1.632788103123879
1201	25	2.0594713145619963
1202	1	0.8480375851022117
1202	2	1.064631789712923
1202	3	0.9421766612112208
1202	4	1.4197219878629
1202	5	1.128729449297687
1202	6	0.8932677838553857
1202	7	0.8105346529000812
1202	8	0.9724506895610432
1202	9	1.1997693642078389
1202	10	1.4397599029203292
1202	11	1.065112137590539
1202	12	0.985703663657155
1202	13	1.0280625147227909
1202	14	1.877923049659728
1202	15	1.4987031801967845
1202	16	1.5755705154889341
1202	17	1.93612722852275
1202	18	1.988619040256971
1202	19	1.5813024626949996
1202	20	1.3809527780868365
1202	21	1.21118760397286
1202	22	1.4021548140228788
1202	23	1.7385876187800875
1202	24	1.3876447864153576
1202	25	1.7652809353541983
1203	1	0.7945312126078363
1203	2	0.9169940000638306
1203	3	0.8512596451220631
1203	4	1.1919639797282855
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2211	13	-0.7029842559990698
2211	14	-0.10886220222789689
2211	15	-0.3734082329091919
2211	16	-6.724300283621943
2211	17	-2.0377958380975585
2211	18	-0.8014894479032222
2211	19	-0.8410881190336994
2211	20	-0.8946052547586021
2211	21	-0.7647493730510249
2211	22	-0.5911765260541557
2211	23	-0.2570309152565642
2211	24	-0.641089220746835
2211	25	-7.956712302907121
2212	1	-1.4977866075856419
2212	2	-4.000957056035919
2212	3	-1.7552622087757364
2212	4	-3.8130841498801047
2212	5	-1.3702857331189833
2212	6	-1.141918804581401
2212	7	-1.0864721451692425
2212	8	-0.900281681615823
2212	9	-0.9557179517965011
2212	10	-0.39122295600058266
2212	11	-0.851532331785549
2212	12	-0.9986810526270204
2212	13	-1.1971361145845734
2212	14	-6.188911229742011
2212	15	-5.032925878296194
2212	16	-2.833522448884345
2212	17	-7.348724677780567
2212	18	-3.0013056956880355
2212	19	-1.841926160386528
2212	20	-1.9713769302632969
2212	21	-1.5776226292033826
2212	22	-1.424543000412008
2212	23	-0.9173092140442307
2212	24	-1.2046941666093431
2212	25	-1.0308273959051775
2213	1	-0.5716890680079203
2213	2	-0.4545746585132804
2213	3	-0.507428161913861
2213	4	-0.17513192871707162
2213	5	-0.2864747350641892
2213	6	-0.6472015261604227
2213	7	-0.6621518029424678
2213	8	-0.7228027170182614
2213	9	-0.5133978682495837
2213	10	-4.253367396316875
2213	11	-1.8895974260790709
2213	12	-1.3995495535654712
2213	13	-1.2129484606008774
2213	14	-0.39304310072945725
2213	15	-0.8960109280331849
2213	16	-0.7346706302452262
2213	17	-0.3362324818456912
2213	18	-0.30983554622485343
2213	19	-0.8830688678618088
2213	20	-1.16186839451494
2213	21	-1.1336079276302442
2213	22	-1.3659602559286341
2213	23	-1.686504613703626
2213	24	-1.120080387608695
2213	25	-0.8122409537522159
2214	1	-0.9966652694384734
2214	2	-0.7985841113069903
2214	3	-0.7915776887638266
2214	4	-0.3370631149825911
2214	5	-3.6725861345163615
2214	6	-1.5343439241279668
2214	7	-1.21140776423616
2214	8	-0.8994476589243341
2214	9	-0.7603740585102656
2214	10	-4.427577931856426
2214	11	-1.9849131280892038
2214	12	-1.4204777545862015
2214	13	-1.1610517873163815
2214	14	-0.42759334676516403
2214	15	-0.756021319866548
2214	16	-0.7023065016368477
2214	17	-0.3232837105413253
2214	18	-0.010570533482535893
2214	19	-0.6528139289966362
2214	20	-1.0072263687159975
2214	21	-1.0284588377533979
2214	22	-0.8429043867286126
2214	23	-0.569604625085347
2214	24	-1.050182838452102
2214	25	-0.8895942485469206
2215	1	-2.054752741601135
2215	2	-4.559331121813287
2215	3	-2.7987372857049664
2215	4	-1.7197410722335889
2215	5	-1.742947261779399
2215	6	-1.9874160034740136
2215	7	-2.099783713078118
2215	8	-2.2482159871897736
2215	9	-7.880823922159003
2215	10	-3.65209790750502
2215	11	-2.9956473372577577
2215	12	-3.4482209169939466
2215	13	-2.7783090606938643
2215	14	-7.857651648117052
2215	15	-4.676389724297014
2215	16	-9.250813668532079
2215	17	-4.41411862387967
2215	18	-2.9332241233695378
2215	19	-3.436596166484616
2215	20	-3.195939582939708
2215	21	-3.3117482882730513
2215	22	-9.25230599893214
2215	23	-10.399797121065536
2215	24	-6.406615401544631
2215	25	-4.857233499292953
2216	1	-1.9620676487836413
2216	2	-1.9571565131669892
2216	3	-1.8381104853908994
2216	4	-6.980104650025954
2216	5	-3.5604080999025634
2216	6	-2.8117668897908032
2216	7	-2.251578724745606
2216	8	-7.668292148236208
2216	9	-3.889807098103528
2216	10	-2.213250127945564
2216	11	-2.2313527392016823
2216	12	-2.3960862646455467
2216	13	-2.138608974357865
2216	14	-1.4118771530774978
2216	15	-2.1001238226778156
2216	16	-1.7497582470771817
2216	17	-1.4735397287127228
2216	18	-7.4499088652413405
2216	19	-4.541938806593741
2216	20	-4.049963448748748
2216	21	-3.131229052106523
2216	22	-2.789300309008649
2216	23	-2.0525473155457306
2216	24	-2.4710574065100843
2216	25	-1.9715558695052238
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	130933.0
3	3.0
4	1095.0
5	6475.0
6	7987.0
7	6875.0
8	6368.0
9	6030.0
10	5697.0
11	5279.0
12	5148.0
13	5194.0
14	5214.0
15	5138.0
16	5216.0
17	5696.0
18	6399.0
19	8112.0
20	10195.0
21	12977.0
22	16172.0
23	19246.0
24	22261.0
25	25637.0
26	28783.0
27	32152.0
28	36525.0
29	43065.0
30	51519.0
31	61716.0
32	79364.0
33	102918.0
34	135819.0
35	182953.0
36	262579.0
37	382307.0
38	1048704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67021117387054	18.506517976951507	12.345371819851476	25.47789902932648
2	14.094887797201155	19.69037297459239	42.30722306645539	23.90751616175106
3	18.93114081648344	24.326692999874027	29.254297134016728	27.487869049625814
4	11.230374190272014	16.360981428839608	39.241057590020915	33.16758679086746
5	12.435016406744325	38.2498221444667	36.4780525730489	12.837108875740071
6	32.283277726127366	37.147461956533476	17.932621781941496	12.636638535397655
7	28.483319164534144	31.286580976937806	21.966931787043848	18.263168071484202
8	25.04357701294114	37.33476111587399	20.421371081714508	17.20029078947037
9	26.472919171416915	15.09176612753099	20.10357108521714	38.33174361583496
10	13.714300117566783	29.113316013387852	35.69399509208198	21.478388776963392
11	34.52339414706127	22.412067828785357	24.458576341591023	18.60596168256235
12	23.41743206814324	24.687915695285586	31.81250526652392	20.08214697004725
13	29.147563180145337	21.7728611657268	25.55920460356214	23.52037105056572
14	20.03902729209721	22.048259454071633	28.666550715494353	29.246162538336804
15	23.041816385632597	31.117582676022504	23.128505422656776	22.712095515688123
16	21.743384073381385	28.183627746598866	28.336301591336767	21.73668658868298
17	21.264201813596717	28.775163132225163	28.440507069483274	21.520127984694852
18	21.121112794063524	27.44342002819005	30.437730880784308	20.997736296962117
19	23.224146728705083	27.111018444797587	27.7890909902776	21.875743836219733
20	23.61854339549804	27.340359332844542	28.072536374780157	20.968560896877264
21	22.774703281166325	26.966922561939395	27.13722730623559	23.121146850658693
22	22.852213812489982	28.268873726159672	27.252250548793555	21.626661912556795
23	21.700631596081	27.961891770525256	28.17122343247028	22.166253200923464
24	22.861238573414685	27.17337434538002	28.327018246518897	21.638368834686396
25	22.37590479987352	27.454812676399737	28.06175201739895	22.10753050632779
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	160.0
2	318.0
3	476.0
4	476.0
5	476.0
6	1028.0
7	1580.0
8	1580.0
9	1580.0
10	2536.0
11	3492.0
12	3492.0
13	3492.0
14	6699.5
15	9907.0
16	9907.0
17	9907.0
18	19193.0
19	28479.0
20	28479.0
21	28479.0
22	47281.5
23	66084.0
24	66084.0
25	66084.0
26	97089.5
27	128095.0
28	128095.0
29	128095.0
30	167776.0
31	207457.0
32	207457.0
33	207457.0
34	241744.0
35	276031.0
36	276031.0
37	276031.0
38	300094.5
39	324158.0
40	324158.0
41	324158.0
42	340869.0
43	357580.0
44	357580.0
45	357580.0
46	368735.5
47	379891.0
48	379891.0
49	379891.0
50	360624.5
51	341358.0
52	341358.0
53	341358.0
54	300506.0
55	259654.0
56	259654.0
57	259654.0
58	220732.5
59	181811.0
60	181811.0
61	181811.0
62	146353.0
63	110895.0
64	110895.0
65	110895.0
66	84675.5
67	58456.0
68	58456.0
69	58456.0
70	42226.5
71	25997.0
72	25997.0
73	25997.0
74	18221.5
75	10446.0
76	10446.0
77	10446.0
78	7105.5
79	3765.0
80	3765.0
81	3765.0
82	2580.5
83	1396.0
84	1396.0
85	1396.0
86	911.0
87	426.0
88	426.0
89	426.0
90	271.5
91	117.0
92	117.0
93	117.0
94	72.5
95	28.0
96	28.0
97	28.0
98	20.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1702096408209375
2	0.2635585407043324
3	0.2648905535449362
4	0.4711725421033059
5	0.26078651398199476
6	0.01918818497410315
7	0.018792181156626352
8	0.01742416796897922
9	0.019008183238886422
10	0.02181621030826737
11	0.020952201979227078
12	0.02797226965267945
13	0.02775626757041938
14	0.025920249871208757
15	0.04176040257028078
16	0.0212042044085305
17	0.04208440569367088
18	0.03186030713336077
19	0.02743226444702927
20	0.02977228700484673
21	0.03470433454978506
22	0.04341641853427467
23	0.029448283881456618
24	0.04791646191469286
25	0.03715235814873255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2777751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.73598393963282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07133715758721	56.535894659860595
2	12.512766807953874	17.451802103196837
3	3.479855286545409	7.280133971243309
4	1.274259013235557	3.554468243277086
5	0.5724956786136723	1.9961774724656125
6	0.31299096212745525	1.3096039624902265
7	0.19112414558892424	0.9329761243085686
8	0.11977760009438777	0.6682247037207991
9	0.08477764027777145	0.5320846944765575
>10	0.3453828025897703	4.142541744236553
>50	0.019560843758814703	0.9442938167401642
>100	0.012746247073989934	1.8028202674146863
>500	0.001567400653599008	0.7415927441089878
>1k	0.0013061672111053543	1.9223334365089761
>5k	5.224668844421417E-5	0.1850520559509693
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5079	0.18284576263315178	No Hit
GTCCTACAGTGGACATTTCTAAATT	4848	0.17452968246613895	No Hit
CCCATGTACTCTGCGTTGATACCAC	4223	0.15202946556404803	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4054	0.14594540691372265	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3837	0.13813333160531668	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3727	0.13417329343054868	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3184	0.11462510498601207	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3141	0.1130770900631482	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3082	0.11095306958759082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.60003470433455E-5	0.0	0.0	0.0	0.0
3	3.60003470433455E-5	0.0	0.0	0.0	0.0
4	3.60003470433455E-5	0.0	0.0	3.60003470433455E-5	0.0
5	3.60003470433455E-5	0.0	0.0	3.60003470433455E-5	0.0
6	3.60003470433455E-5	0.0	0.0	3.60003470433455E-5	0.0
7	3.60003470433455E-5	0.0	0.0	3.60003470433455E-5	0.0
8	3.60003470433455E-5	0.0	0.0	3.60003470433455E-5	0.0
9	3.60003470433455E-5	0.0	0.0	7.2000694086691E-5	0.0
10	3.60003470433455E-5	0.0	0.0	7.2000694086691E-5	0.0
11	3.60003470433455E-5	0.0	0.0	7.2000694086691E-5	0.0
12	3.60003470433455E-5	0.0	0.0	7.2000694086691E-5	0.0
13	3.60003470433455E-5	0.0	0.0	7.2000694086691E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAC	40	0.0053224782	14.232996	11
TAGGACC	1095	0.0	14.187787	4
GTAGGAC	2865	0.0	12.175802	3
TAGGACT	690	0.0	12.155553	4
TGTAGGA	3105	0.0	11.887893	2
GGACCTG	1755	0.0	11.783941	6
AGGACGT	2200	0.0	11.672504	5
GGACGTG	2155	0.0	11.489587	6
CTGTAGG	3110	0.0	11.488388	1
AGGACCT	1790	0.0	11.476876	5
GGACCGC	75	2.102285E-4	11.383937	6
GTCCTAC	2730	0.0	11.337838	1
GGCGAGG	1080	0.0	11.246634	19
AGGACCG	110	4.8995935E-7	11.24019	5
GACGGTG	60	0.0059380494	11.068113	7
GTCCTAA	1360	0.0	11.028302	1
GACGTGG	1270	0.0	10.980964	7
TTAGGAC	1470	0.0	10.892639	3
TAGGACG	2320	0.0	10.763524	4
TGGCGAG	2665	0.0	10.681999	18
>>END_MODULE