FastQCFastQC Report
Thu 26 May 2016
SRR1513157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC55710.20055793337847774No Hit
CCCATGTACTCTGCGTTGATACCAC51700.18612179421409622No Hit
GTCCTACAGTGGACATTTCTAAATT48570.17485368558952907No Hit
GTCCTACAGTGTGCATTTCTCATTT42720.15379348256917197No Hit
GTATCAACGCAGAGTACTTTTTTTT42710.15375748222212862No Hit
CTGTAGGACGTGGAATATGGCAAGA41080.1478894256540633No Hit
GTCCTAAAGTGTGTATTTCTCATTT38810.13971734687522386No Hit
CTTTAGGACGTGAAATATGGCGAGG37150.13374128926602852No Hit
CTGTAGGACCTGGAATATGGCGAGA34740.12506520562858225No Hit
TATCAACGCAGAGTACTTTTTTTTT30060.10821704321229655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11900.014.5588684
GTAGGAC27750.012.1443653
GGCGAGG10400.012.04342519
AGGACCT19550.011.9781995
GTCCTAC28300.011.8464151
TGTAGGA30200.011.7274052
AGGACGT23400.011.3092065
TGGCGAG26000.011.20685918
CTGTAGG29850.011.1993671
AGGGTCG600.005789683711.1058035
GGACGTG23250.011.0947666
TTAGGAC15900.010.8970473
AACCGAG700.001503461310.8472247
TAGGACG25450.010.8470944
ACCGTCC700.001505268710.8456618
GACGTGG11000.010.7855937
TACACCG1158.596908E-710.7609025
GGACCTG20100.010.749836
TCCTACA32450.010.7088812
ATAGCGA803.8034175E-410.67812117
Produced by FastQC (version 0.11.5)