##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513157_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2777751 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.668021719729378 31.0 31.0 34.0 27.0 34.0 2 30.77240724600585 33.0 31.0 34.0 27.0 34.0 3 30.8883262034646 33.0 31.0 34.0 27.0 34.0 4 34.26900503320852 37.0 35.0 37.0 31.0 37.0 5 34.00222554145422 37.0 35.0 37.0 30.0 37.0 6 33.91127570469779 37.0 35.0 37.0 30.0 37.0 7 33.774111502434884 37.0 35.0 37.0 29.0 37.0 8 33.73743398886365 37.0 35.0 37.0 29.0 37.0 9 35.28464142394333 39.0 35.0 39.0 29.0 39.0 10 35.12649009936456 39.0 35.0 39.0 28.0 39.0 11 35.14181616710785 39.0 35.0 39.0 28.0 39.0 12 35.06835709896244 39.0 35.0 39.0 27.0 39.0 13 35.041906923982744 39.0 35.0 39.0 27.0 39.0 14 36.20284539542961 40.0 36.0 41.0 27.0 41.0 15 36.19389138911299 40.0 37.0 41.0 27.0 41.0 16 36.15506141479204 40.0 36.0 41.0 27.0 41.0 17 36.09640316932655 40.0 36.0 41.0 27.0 41.0 18 36.05093581102122 40.0 36.0 41.0 27.0 41.0 19 36.12312334690906 40.0 37.0 41.0 27.0 41.0 20 36.09307889728057 40.0 37.0 41.0 27.0 41.0 21 36.04451550912951 40.0 37.0 41.0 27.0 41.0 22 35.98583962349397 40.0 37.0 41.0 27.0 41.0 23 35.90715366496133 40.0 37.0 41.0 27.0 41.0 24 35.85021299605328 40.0 37.0 41.0 27.0 41.0 25 35.79410033512723 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5543282373227392 1101 2 0.5774632371954169 1101 3 0.539387072080789 1101 4 0.6572930967039881 1101 5 0.5907095645450156 1101 6 0.5658403199052273 1101 7 0.5836203508890137 1101 8 0.6185496632636358 1101 9 0.6885144207899287 1101 10 0.7698706409715754 1101 11 0.704189512925609 1101 12 0.7566820733980393 1101 13 0.6904195275758838 1101 14 0.7760069418931366 1101 15 0.7569153596946592 1101 16 0.742171335655307 1101 17 0.797076585747007 1101 18 0.7823722677414011 1101 19 0.814168400374264 1101 20 0.8820155329026846 1101 21 0.8281509907820919 1101 22 0.916411175882601 1101 23 0.929919553093292 1101 24 0.9625917618952684 1101 25 1.016447704740358 1102 1 0.593232312178614 1102 2 0.6249874253013772 1102 3 0.5250644325120852 1102 4 0.6051946053631099 1102 5 0.6665712583307339 1102 6 0.6962789890489418 1102 7 0.7078686215580632 1102 8 0.7383095460449027 1102 9 0.7086246033282819 1102 10 0.8200030248983836 1102 11 0.8425104059083992 1102 12 0.8302187205474212 1102 13 0.8218672527004571 1102 14 0.9048861336513951 1102 15 0.7882503763457009 1102 16 0.8727573909593858 1102 17 1.0455823536984 1102 18 1.0124811873121757 1102 19 0.9824795011643488 1102 20 0.9975493272311695 1102 21 1.0352768959487975 1102 22 0.9895691724086078 1102 23 1.0424488865339896 1102 24 1.0656366760901577 1102 25 1.0386576008462924 1103 1 0.4870035473930301 1103 2 0.4430451731985805 1103 3 0.4986927248933988 1103 4 0.6114970751941158 1103 5 0.5025574084195341 1103 6 0.40836428636929867 1103 7 0.4040553769391053 1103 8 0.4668371409526557 1103 9 0.5285385109396046 1103 10 0.6119926292271529 1103 11 0.529339339004629 1103 12 0.4319049691253696 1103 13 0.5772445847566914 1103 14 0.602862440003598 1103 15 0.6131051581061513 1103 16 0.5885776028999317 1103 17 0.6469893398257724 1103 18 0.5888355778345229 1103 19 0.5811762410900414 1103 20 0.5878772552532396 1103 21 0.5154467906093103 1103 22 0.602238684892626 1103 23 0.6359605006391789 1103 24 0.6422437914808654 1103 25 0.6441466673035805 1104 1 0.2897793130858375 1104 2 0.35944322603851475 1104 3 0.34211092038091806 1104 4 0.44800398572586886 1104 5 0.41298376945065485 1104 6 0.3347270556528059 1104 7 0.351518174160681 1104 8 0.4063277362724307 1104 9 0.42346393156643813 1104 10 0.5154206233206438 1104 11 0.48533049244863946 1104 12 0.5196779867819785 1104 13 0.47440736919697457 1104 14 0.593739747806481 1104 15 0.5292032039586729 1104 16 0.5752430513072468 1104 17 0.6442305849579029 1104 18 0.5362829054026435 1104 19 0.5728852600370686 1104 20 0.5540935599435386 1104 21 0.5415556837596611 1104 22 0.5303189912821509 1104 23 0.5209824461097554 1104 24 0.4655718319137847 1104 25 0.42760958457430576 1105 1 0.2792799438051006 1105 2 0.3883523989173483 1105 3 0.2357666457609895 1105 4 0.37516537455380927 1105 5 0.4139206752529532 1105 6 0.37321125231626695 1105 7 0.38217509038570796 1105 8 0.3296449736121545 1105 9 0.3932956461418513 1105 10 0.3450204153312768 1105 11 0.35428596866363904 1105 12 0.40757347433497415 1105 13 0.466770528586153 1105 14 0.5020367778813792 1105 15 0.40336766011805736 1105 16 0.3465669767774173 1105 17 0.39065643644053694 1105 18 0.3857395529534102 1105 19 0.43364263603326236 1105 20 0.37308570818213127 1105 21 0.42320369827471893 1105 22 0.34079810125420096 1105 23 0.3901303973871606 1105 24 0.32758971604530984 1105 25 0.38411714026719324 1106 1 0.1642978166850213 1106 2 0.23163175764155497 1106 3 0.173424946544241 1106 4 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16 0.37690537158549375 1107 17 0.39904968920937733 1107 18 0.40413140267753533 1107 19 0.338611915879774 1107 20 0.352631709015256 1107 21 0.3801389265522701 1107 22 0.417926405886746 1107 23 0.3664961635311528 1107 24 0.39001432958262683 1107 25 0.4124901848416016 1108 1 0.18381888692903914 1108 2 0.20391724578525228 1108 3 0.22498381211896756 1108 4 0.2930788578588448 1108 5 0.029240419352937863 1108 6 0.08638657974774588 1108 7 0.05865241364253393 1108 8 0.19854996152180604 1108 9 0.22833561501564503 1108 10 0.25294076146787603 1108 11 0.26707724854956894 1108 12 0.22711350384422957 1108 13 0.21395023325472096 1108 14 0.2367412535132729 1108 15 0.304334680859057 1108 16 0.246447612200285 1108 17 0.370742626071511 1108 18 0.383049313792462 1108 19 0.3405381510304366 1108 20 0.36185700868411885 1108 21 0.26123863394601443 1108 22 0.17869195612598077 1108 23 0.29590283572274245 1108 24 0.28451672013912344 1108 25 0.3416963774914876 1109 1 0.14396783552717096 1109 2 0.07618784901379883 1109 3 0.10140736422885155 1109 4 0.22977083605199056 1109 5 0.12721151465171943 1109 6 0.15417279470101874 1109 7 0.15373082627351664 1109 8 0.023197724195270553 1109 9 -0.00705184604354514 1109 10 0.11486107441038484 1109 11 0.13656358386534606 1109 12 0.16000875306541928 1109 13 0.19381473693639606 1109 14 0.2723951051133895 1109 15 0.25049104591145266 1109 16 0.17410709251747392 1109 17 0.22328528395879488 1109 18 0.19275796573241877 1109 19 0.20326206068775576 1109 20 0.18446834399989598 1109 21 0.19227224694515854 1109 22 0.24710741916761236 1109 23 0.16938989675360716 1109 24 0.17081486514053523 1109 25 0.2597299550899024 1110 1 0.2629853116851919 1110 2 0.2943645075341834 1110 3 0.3152963736961887 1110 4 0.22311591854935386 1110 5 0.3496153762488845 1110 6 0.3197165263339059 1110 7 0.30739469889636695 1110 8 0.3833269288260652 1110 9 0.3646255786323067 1110 10 0.4904729135741732 1110 11 0.44042724291965385 1110 12 0.41633832579809393 1110 13 0.36368833213717977 1110 14 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-0.9318190859841096 2215 24 -0.9993295534159614 2215 25 -1.1127904643350348 2216 1 -0.6681704204235999 2216 2 -0.650536487480867 2216 3 -0.6991394386418754 2216 4 -0.601069709608808 2216 5 -0.818858318720963 2216 6 -0.7528161569538341 2216 7 -1.1159380496782916 2216 8 -1.0321529782268826 2216 9 -1.066857828196511 2216 10 -1.0898759803495537 2216 11 -1.063340491699499 2216 12 -1.0384858235330512 2216 13 -1.0579413664297448 2216 14 -1.2180895172255362 2216 15 -1.094041117326121 2216 16 -1.2061102562251946 2216 17 -1.4164450431344164 2216 18 -1.3890962406428358 2216 19 -1.321754542541143 2216 20 -1.2970130575045715 2216 21 -1.5901374478049561 2216 22 -1.447159908173198 2216 23 -1.29060150411744 2216 24 -1.4352531831451074 2216 25 -1.508282004993248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72928.0 3 0.0 4 1183.0 5 10062.0 6 11695.0 7 8855.0 8 6858.0 9 6050.0 10 5423.0 11 4823.0 12 4541.0 13 4113.0 14 3991.0 15 4123.0 16 4239.0 17 4320.0 18 4818.0 19 5790.0 20 7303.0 21 9673.0 22 11836.0 23 14388.0 24 16786.0 25 18249.0 26 20432.0 27 22534.0 28 25182.0 29 30445.0 30 38948.0 31 49858.0 32 62959.0 33 82719.0 34 110000.0 35 148825.0 36 214876.0 37 352614.0 38 1376312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.8573156133766 19.40553822583315 13.293898747535337 27.443247413254912 2 13.47081143571452 20.07899884392814 40.52220892893597 25.92798079142138 3 17.303375478967205 24.16493285008484 29.6774435113017 28.854248159646257 4 11.066593896681502 16.816670758778297 37.71254079811297 34.40419454642723 5 12.42629473746407 38.3612518023247 36.270663233873115 12.941790226338115 6 32.403214446952596 37.46080361173815 17.564677200902935 12.571304740406319 7 28.187287221898526 31.333072135872612 22.0062341688051 18.473406473423758 8 25.011188727091916 37.77256657337627 19.95554033720645 17.26070436232537 9 26.09337585905596 14.900671907857573 19.858490005627132 39.147462227459336 10 14.142885121347446 29.41532464203476 34.9294222009732 21.512368035644595 11 34.42673587373382 22.343687393146467 24.403375248537394 18.82620148458231 12 23.120790884424125 24.80780314722234 31.7889904458679 20.282415522485636 13 28.942911009662133 21.669004889207134 25.661011372149627 23.727072728981106 14 20.367583633700878 22.134220011606693 27.63073008393971 29.867466270752725 15 22.966277394914087 31.167084450694105 22.971281443153114 22.895356711238694 16 21.918811297340905 28.337853176904627 27.813472121871254 21.929863403883214 17 21.476880532765858 29.050178146733856 27.808390090036568 21.664551230463722 18 21.409730147063854 27.666794984582044 29.75379165035685 21.169683217997253 19 23.194879598639332 27.13299356205794 27.648950535883166 22.023176303419564 20 23.474602295166125 27.363179781053088 28.085742746560076 21.076475177220708 21 22.517375723883493 26.922057827157897 27.238899733091028 23.321666715867583 22 22.804400253586625 28.255792730012686 27.095536709760314 21.844270306640375 23 21.677781141982187 27.960846901057707 27.850788213891875 22.51058374306823 24 22.72892710685731 27.203086237751332 28.387173652354008 21.68081300303735 25 22.321299355664628 27.411397586388386 27.87299486706128 22.394308190885702 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 125.0 1 125.0 2 261.0 3 397.0 4 397.0 5 397.0 6 1009.0 7 1621.0 8 1621.0 9 1621.0 10 2559.5 11 3498.0 12 3498.0 13 3498.0 14 6448.5 15 9399.0 16 9399.0 17 9399.0 18 18460.0 19 27521.0 20 27521.0 21 27521.0 22 46018.0 23 64515.0 24 64515.0 25 64515.0 26 94790.5 27 125066.0 28 125066.0 29 125066.0 30 164313.0 31 203560.0 32 203560.0 33 203560.0 34 237945.5 35 272331.0 36 272331.0 37 272331.0 38 295658.0 39 318985.0 40 318985.0 41 318985.0 42 336210.0 43 353435.0 44 353435.0 45 353435.0 46 367946.0 47 382457.0 48 382457.0 49 382457.0 50 364828.0 51 347199.0 52 347199.0 53 347199.0 54 305161.0 55 263123.0 56 263123.0 57 263123.0 58 224583.5 59 186044.0 60 186044.0 61 186044.0 62 150341.5 63 114639.0 64 114639.0 65 114639.0 66 87815.5 67 60992.0 68 60992.0 69 60992.0 70 43857.0 71 26722.0 72 26722.0 73 26722.0 74 18611.0 75 10500.0 76 10500.0 77 10500.0 78 7117.0 79 3734.0 80 3734.0 81 3734.0 82 2550.5 83 1367.0 84 1367.0 85 1367.0 86 888.5 87 410.0 88 410.0 89 410.0 90 256.0 91 102.0 92 102.0 93 102.0 94 54.5 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18208975534524152 2 0.00871208398448961 3 0.005760055526935279 4 0.0017640170051239296 5 0.07808475273701639 6 0.3240391237371528 7 0.02869227659354636 8 0.01447213951142489 9 0.004968047891981678 10 0.0038520371336379687 11 0.0 12 0.0 13 0.0 14 0.0015480149228638564 15 0.0 16 0.0 17 0.01965618948566664 18 0.028584275552416327 19 0.0 20 0.0 21 9.000086760836374E-4 22 7.2000694086691E-5 23 0.005112049280155061 24 0.0 25 1.8000173521672748E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2777751.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.08909224231859 #Duplication Level Percentage of deduplicated Percentage of total 1 79.34514311678623 53.23193625542041 2 13.41456573065635 17.99942075389299 3 3.867431876520949 7.783874817143915 4 1.4481646610926069 3.886242101204317 5 0.6766945570785667 2.269941177985944 6 0.3651871355377612 1.4700044053080576 7 0.21980159846456435 1.032240280007876 8 0.14044246739752472 0.7537726119977084 9 0.08623194102515089 0.5206700381103472 >10 0.3954227745132954 4.57099525357616 >50 0.022232070475135982 1.0207933330601227 >100 0.015248199008925913 2.048718463023105 >500 0.001770589962913518 0.811065103910454 >1k 0.0015559729974393979 2.213690828759266 >5k 1.0730848258202744E-4 0.38663457659929223 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 5571 0.20055793337847774 No Hit CCCATGTACTCTGCGTTGATACCAC 5170 0.18612179421409622 No Hit GTCCTACAGTGGACATTTCTAAATT 4857 0.17485368558952907 No Hit GTCCTACAGTGTGCATTTCTCATTT 4272 0.15379348256917197 No Hit GTATCAACGCAGAGTACTTTTTTTT 4271 0.15375748222212862 No Hit CTGTAGGACGTGGAATATGGCAAGA 4108 0.1478894256540633 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3881 0.13971734687522386 No Hit CTTTAGGACGTGAAATATGGCGAGG 3715 0.13374128926602852 No Hit CTGTAGGACCTGGAATATGGCGAGA 3474 0.12506520562858225 No Hit TATCAACGCAGAGTACTTTTTTTTT 3006 0.10821704321229655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.60003470433455E-5 0.0 5 0.0 0.0 0.0 3.60003470433455E-5 0.0 6 0.0 0.0 0.0 3.60003470433455E-5 0.0 7 0.0 0.0 0.0 3.60003470433455E-5 0.0 8 0.0 0.0 0.0 7.2000694086691E-5 0.0 9 0.0 0.0 0.0 1.44001388173382E-4 0.0 10 0.0 0.0 0.0 1.44001388173382E-4 0.0 11 0.0 0.0 0.0 1.44001388173382E-4 0.0 12 0.0 0.0 0.0 1.44001388173382E-4 0.0 13 0.0 0.0 0.0 1.44001388173382E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1190 0.0 14.558868 4 GTAGGAC 2775 0.0 12.144365 3 GGCGAGG 1040 0.0 12.043425 19 AGGACCT 1955 0.0 11.978199 5 GTCCTAC 2830 0.0 11.846415 1 TGTAGGA 3020 0.0 11.727405 2 AGGACGT 2340 0.0 11.309206 5 TGGCGAG 2600 0.0 11.206859 18 CTGTAGG 2985 0.0 11.199367 1 AGGGTCG 60 0.0057896837 11.105803 5 GGACGTG 2325 0.0 11.094766 6 TTAGGAC 1590 0.0 10.897047 3 AACCGAG 70 0.0015034613 10.847224 7 TAGGACG 2545 0.0 10.847094 4 ACCGTCC 70 0.0015052687 10.845661 8 GACGTGG 1100 0.0 10.785593 7 TACACCG 115 8.596908E-7 10.760902 5 GGACCTG 2010 0.0 10.74983 6 TCCTACA 3245 0.0 10.708881 2 ATAGCGA 80 3.8034175E-4 10.678121 17 >>END_MODULE