FastQCFastQC Report
Thu 26 May 2016
SRR1513156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685328
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34420.12817801028403233No Hit
CCCATGTACTCTGCGTTGATACCAC28750.10706327122794682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6450.013.0000234
GGACCGC1302.0190782E-1012.4059426
AACCGTC600.00593576411.0686657
GTAGGAC13050.010.8061853
TGGCGAG13550.010.5052318
TGTAGGA14800.010.5014142
GTCCTAC13750.010.3497461
GCGAATC750.002672939310.12218515
TACACCG856.52989E-410.0698955
TACCGTA951.6669597E-49.9867657
GTCCTAA6600.09.9850721
GTCTAGC1752.3483153E-99.8238241
TATGGCG15300.09.73818516
GGCGAGG6550.09.70722819
ACTGTTC5400.09.663128
GTATTAC2700.09.550941
GTATAGG3000.09.550941
AGCACCG1502.6080306E-79.5104565
AGGACCG1701.6365448E-89.5104565
TCCTACA15850.09.5049482
Produced by FastQC (version 0.11.5)