##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513156_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2685328 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.557306593458975 31.0 30.0 34.0 25.0 34.0 2 29.39089154099611 31.0 30.0 34.0 23.0 34.0 3 29.569169948699006 31.0 30.0 34.0 24.0 34.0 4 32.64763894764438 37.0 33.0 37.0 23.0 37.0 5 32.772808014514425 37.0 33.0 37.0 25.0 37.0 6 33.14571888424803 37.0 35.0 37.0 27.0 37.0 7 33.10341902367234 37.0 35.0 37.0 26.0 37.0 8 32.99243518855052 37.0 35.0 37.0 26.0 37.0 9 34.24131614461995 39.0 35.0 39.0 25.0 39.0 10 33.80634991330668 39.0 34.0 39.0 19.0 39.0 11 34.287163430314656 39.0 35.0 39.0 26.0 39.0 12 34.27695536634631 39.0 35.0 39.0 26.0 39.0 13 34.33040023416134 39.0 35.0 39.0 26.0 39.0 14 34.55823944039611 39.0 34.0 41.0 17.0 41.0 15 35.002611971423974 40.0 34.0 41.0 23.0 41.0 16 34.8484218687624 40.0 34.0 41.0 19.0 41.0 17 34.45354943604654 39.0 34.0 41.0 17.0 41.0 18 34.18522057640631 39.0 33.0 41.0 17.0 41.0 19 34.82646887084185 39.0 34.0 41.0 22.0 41.0 20 35.04009752253729 40.0 35.0 41.0 24.0 41.0 21 35.08973168268457 40.0 35.0 41.0 24.0 41.0 22 34.779206860390985 40.0 35.0 41.0 18.0 41.0 23 34.313470831123794 39.0 34.0 41.0 15.0 41.0 24 34.56242291444472 40.0 34.0 41.0 18.0 41.0 25 34.20596143189957 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1568442970746204 1101 2 1.3884882212860532 1101 3 1.261343101010759 1101 4 1.699584267931229 1101 5 1.4925320147310472 1101 6 1.189163800609201 1101 7 1.1325044688841786 1101 8 1.232638559554708 1101 9 1.5814035773942479 1101 10 1.859878937236985 1101 11 1.4418804189161705 1101 12 1.3412056371310186 1101 13 1.1933591214054786 1101 14 2.296656348843321 1101 15 1.9366354941462731 1101 16 2.0626176267670715 1101 17 2.3670507800390794 1101 18 2.477905898274308 1101 19 1.9933975012294525 1101 20 1.6076897402511392 1101 21 1.6748760147016526 1101 22 1.9230125070183135 1101 23 2.128628179576907 1101 24 1.81714883730573 1101 25 2.12503502447543 1102 1 0.8049045465083182 1102 2 0.9608750753689534 1102 3 0.8371550499734361 1102 4 1.1866649960227136 1102 5 1.209830138708945 1102 6 0.9174701485343704 1102 7 0.8747651182945688 1102 8 0.8666839406670306 1102 9 1.188996191339868 1102 10 1.488542300934327 1102 11 0.9800346740308754 1102 12 0.9357857421961953 1102 13 1.0009442962309976 1102 14 1.9898785045267502 1102 15 1.4815051049687042 1102 16 1.6465405723610687 1102 17 1.9398317215093215 1102 18 2.2196489089110116 1102 19 1.535506888531124 1102 20 1.3817543018947873 1102 21 1.2525640477610978 1102 22 1.4477592979678775 1102 23 1.7602825035034684 1102 24 1.5759980426217268 1102 25 1.848680639672068 1103 1 0.6992559869737462 1103 2 0.9655356204440046 1103 3 0.7578390944916755 1103 4 1.2118976777320754 1103 5 1.0686009342673017 1103 6 0.8209072846139946 1103 7 0.7286992017623319 1103 8 0.7574567537196373 1103 9 1.1791268374290667 1103 10 1.434953943719023 1103 11 0.9759907741320575 1103 12 0.8944114697338392 1103 13 0.7610069428221422 1103 14 1.677460245476361 1103 15 1.2673819784103486 1103 16 1.3535724006364234 1103 17 1.729119724268827 1103 18 1.8336650379530468 1103 19 1.3588117962919881 1103 20 0.9972240209914531 1103 21 1.1511449515233494 1103 22 1.3038744333576275 1103 23 1.7270144598727342 1103 24 1.405413872555691 1103 25 1.6659027537853675 1104 1 0.708845778293135 1104 2 0.8667338954547041 1104 3 0.7979714314571424 1104 4 1.1465309379439006 1104 5 1.0353119925739733 1104 6 0.7459495564284921 1104 7 0.6573632328624157 1104 8 0.7549859848664084 1104 9 1.0389532012749214 1104 10 1.2338250992284259 1104 11 0.8247025686416549 1104 12 0.5905045066307721 1104 13 0.663073099334099 1104 14 1.6081030463993073 1104 15 1.1794485239726313 1104 16 1.2897477964951278 1104 17 1.6883867534505939 1104 18 1.8517385205819537 1104 19 1.0822038961761749 1104 20 0.9606754360872003 1104 21 0.8955197326825441 1104 22 1.170281433869377 1104 23 1.4820157018410853 1104 24 1.1825835014914574 1104 25 1.5817448831476 1105 1 0.5246317638201106 1105 2 0.8129389060825858 1105 3 0.5992076689484875 1105 4 1.0710482052649581 1105 5 0.7461813890536391 1105 6 0.5315374334794356 1105 7 0.5033956896941163 1105 8 0.5058711440457913 1105 9 0.7585096962236904 1105 10 1.170868936251665 1105 11 0.6503578551167806 1105 12 0.685690315424452 1105 13 0.6674132154048849 1105 14 1.41925217109182 1105 15 0.9870271583496475 1105 16 1.1457292633815186 1105 17 1.5068712056967755 1105 18 1.1213535285827163 1105 19 0.9742897073581318 1105 20 0.7834262660172229 1105 21 0.7260510073117601 1105 22 0.8652260255915749 1105 23 1.2273842146542506 1105 24 1.030258503282674 1105 25 1.1956318813086142 1106 1 0.5286519454114043 1106 2 0.8284609451835223 1106 3 0.7558956001560837 1106 4 1.2745984512594788 1106 5 1.126815416622236 1106 6 0.7999733960136837 1106 7 0.692722898265913 1106 8 0.7915570241037457 1106 9 0.8712103287810251 1106 10 0.9415018163239566 1106 11 0.7972526225336267 1106 12 0.7947028231341875 1106 13 0.6981750812338774 1106 14 1.6109604344995958 1106 15 1.1311157594082104 1106 16 1.3427945415776534 1106 17 1.5249014207089004 1106 18 1.7198596318286974 1106 19 1.149171371801529 1106 20 0.9884666435435108 1106 21 0.8240568738866259 1106 22 1.1314469616616876 1106 23 1.5696081342147608 1106 24 1.1362441308914342 1106 25 1.4976545228451101 1107 1 0.5136215397640065 1107 2 0.685319637163623 1107 3 0.6636534316921754 1107 4 0.9692056571372518 1107 5 0.8278425751943672 1107 6 0.5735933700508582 1107 7 0.5230132733806911 1107 8 0.7012151027928084 1107 9 0.9210320525447173 1107 10 1.0903537316062426 1107 11 0.6205608692886955 1107 12 0.598521904847388 1107 13 0.48108648217388605 1107 14 1.3837969236349252 1107 15 0.7637980606296395 1107 16 1.0211807319747024 1107 17 1.5620186165883538 1107 18 1.618325978084421 1107 19 1.1206513187970586 1107 20 0.8504309180096996 1107 21 0.8419514565505892 1107 22 1.1132268658606463 1107 23 1.36592803773771 1107 24 1.0721405182385269 1107 25 1.4147308100829008 1108 1 0.40521850685267324 1108 2 0.6138074970560616 1108 3 0.4093994008719868 1108 4 0.809917888194903 1108 5 0.8162561560950294 1108 6 0.3946110659283093 1108 7 0.30182206435124925 1108 8 0.3321032665154604 1108 9 0.6054583766381114 1108 10 0.839152752556636 1108 11 0.4355660492908058 1108 12 0.22059703723373048 1108 13 0.2993131486084124 1108 14 1.3609167059562566 1108 15 0.8630212887875146 1108 16 0.9764764837482858 1108 17 1.257933376155684 1108 18 1.6064136217187865 1108 19 0.8966794339663409 1108 20 0.7493297474871241 1108 21 0.6175753169631406 1108 22 0.8794410012653415 1108 23 1.3149848238759532 1108 24 1.0602884906339796 1108 25 1.3363387040232055 1109 1 0.6468981668047675 1109 2 0.7667819311323782 1109 3 0.8129461328605174 1109 4 1.0327234976995285 1109 5 1.1036391718806513 1109 6 0.6443694904796331 1109 7 0.6251810132656033 1109 8 0.7162981546211284 1109 9 0.883213053961633 1109 10 1.2817377544513349 1109 11 0.8250559532401525 1109 12 0.7574013031099369 1109 13 0.7381900955417748 1109 14 1.7382940797007578 1109 15 1.1891008490562527 1109 16 1.3071250949616484 1109 17 1.8211566695246546 1109 18 1.97079811328355 1109 19 1.3321209228199962 1109 20 1.1935387516409435 1109 21 1.072567739898929 1109 22 1.186921585074309 1109 23 1.5402969190779388 1109 24 1.3645315544201537 1109 25 1.5989156340085486 1110 1 0.3044604132409283 1110 2 0.49282778397084215 1110 3 0.4379005697683489 1110 4 0.7689724446890267 1110 5 0.677545746530221 1110 6 0.2867168459513252 1110 7 0.25567176001644754 1110 8 0.3149281029678548 1110 9 0.5155900181336506 1110 10 0.8252028518328061 1110 11 0.3037179789210569 1110 12 0.33680566177938687 1110 13 0.15300611811056797 1110 14 0.9635502678250987 1110 15 0.6938440543795181 1110 16 0.8128654999391358 1110 17 1.0876702877725037 1110 18 0.7701748892981968 1110 19 0.5205174723280308 1110 20 0.4130393423129064 1110 21 0.18403475623330934 1110 22 0.4586950674521262 1110 23 -0.7099592031167958 1110 24 -0.04222781271523246 1110 25 0.3883177762965673 1111 1 0.15026379797213352 1111 2 0.43689614213440464 1111 3 0.3429092730585559 1111 4 0.7597803946534398 1111 5 0.603377476725278 1111 6 0.3287903025818366 1111 7 0.35255713561083724 1111 8 0.3646750378936261 1111 9 0.5463153180011489 1111 10 0.9052831496278699 1111 11 0.4988625491715979 1111 12 0.4237394181946783 1111 13 0.2002138474390378 1111 14 1.0718269710400747 1111 15 0.6473185039170914 1111 16 0.7339211282518789 1111 17 1.166238755732813 1111 18 1.4248481969495899 1111 19 0.8648928194795147 1111 20 0.42815469461769595 1111 21 0.3798279657860064 1111 22 0.6705267038270506 1111 23 1.0339064559730247 1111 24 0.7982908811725693 1111 25 0.9002406946737338 1112 1 -0.02357232436631307 1112 2 0.11592662383761976 1112 3 0.025346359649073946 1112 4 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2216 22 -2.7387475678009565 2216 23 -2.070093068942114 2216 24 -2.3830701769039635 2216 25 -1.9444506151045857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118224.0 3 3.0 4 1020.0 5 5826.0 6 7008.0 7 6335.0 8 5741.0 9 5489.0 10 5267.0 11 5089.0 12 4842.0 13 4755.0 14 4870.0 15 5132.0 16 5095.0 17 5415.0 18 6203.0 19 7456.0 20 9630.0 21 12447.0 22 15514.0 23 18496.0 24 21214.0 25 24479.0 26 27991.0 27 31584.0 28 35547.0 29 42133.0 30 50920.0 31 61008.0 32 77957.0 33 101560.0 34 134302.0 35 180308.0 36 257039.0 37 373517.0 38 1005912.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40129898905349 17.748672624469666 12.2317366033361 25.618291783140744 2 14.951622032098658 19.19423317299436 40.77025430727439 25.083890487632594 3 19.235743364127007 23.51471286614159 28.858319220809474 28.391224548921922 4 11.860371250545676 15.699312719275769 37.779716297040835 34.66059973313772 5 13.413917537704314 37.35065926082448 35.53807465524979 13.69734854622143 6 33.71257536327267 35.75594572317269 17.250372370083905 13.281106543470738 7 29.390573669743226 30.87707928278246 21.071468478333742 18.660878569140575 8 26.299040503984028 35.82706947546732 19.787682501088515 18.08620751946014 9 26.67489064492676 15.005035696833172 19.310035041298672 39.010038616941394 10 14.504882749208681 28.296479608217943 34.50765394869854 22.69098369387483 11 35.66184481399111 22.026332775996345 23.188508900692828 19.12331350931972 12 23.73785836359275 23.969925842706672 30.677942488985178 21.614273304715404 13 29.395212763777952 20.898220477784935 25.205831638789732 24.50073511964738 14 21.109716350362245 20.60987465777662 27.76265062484868 30.51775836701246 15 24.028057135044335 29.168437809329056 22.76900288610879 24.03450216951782 16 23.312483541353245 27.238310775753057 26.379909705116923 23.069295977776775 17 22.4232859937494 27.462695908648428 27.34713173979956 22.766886357802612 18 22.49135489465879 26.79120287192951 28.390067749016843 22.327374484394856 19 24.139526342315065 26.42702051741844 26.727512546575895 22.7059405936906 20 24.42898714410876 26.405870976201403 26.721829684771404 22.443312194918434 21 24.005337549331728 25.968697404016872 26.41233835175225 23.613626694899153 22 23.994885478379896 26.534747675403725 26.31910919101293 23.15125765520345 23 23.197565612828434 26.3810952805252 26.94532686467978 23.476012241966583 24 23.7988876353757 26.26765454103782 26.744068208338863 23.18938961524762 25 23.576971062020387 26.137426624997623 26.7874219497103 23.498180363271686 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 161.0 1 161.0 2 303.5 3 446.0 4 446.0 5 446.0 6 890.5 7 1335.0 8 1335.0 9 1335.0 10 2078.0 11 2821.0 12 2821.0 13 2821.0 14 5380.0 15 7939.0 16 7939.0 17 7939.0 18 15333.0 19 22727.0 20 22727.0 21 22727.0 22 36914.0 23 51101.0 24 51101.0 25 51101.0 26 74515.0 27 97929.0 28 97929.0 29 97929.0 30 128643.5 31 159358.0 32 159358.0 33 159358.0 34 189164.0 35 218970.0 36 218970.0 37 218970.0 38 247035.5 39 275101.0 40 275101.0 41 275101.0 42 298986.0 43 322871.0 44 322871.0 45 322871.0 46 342811.5 47 362752.0 48 362752.0 49 362752.0 50 359470.0 51 356188.0 52 356188.0 53 356188.0 54 325609.0 55 295030.0 56 295030.0 57 295030.0 58 257480.5 59 219931.0 60 219931.0 61 219931.0 62 182271.5 63 144612.0 64 144612.0 65 144612.0 66 113044.0 67 81476.0 68 81476.0 69 81476.0 70 60127.5 71 38779.0 72 38779.0 73 38779.0 74 27589.5 75 16400.0 76 16400.0 77 16400.0 78 11373.0 79 6346.0 80 6346.0 81 6346.0 82 4272.5 83 2199.0 84 2199.0 85 2199.0 86 1422.5 87 646.0 88 646.0 89 646.0 90 404.5 91 163.0 92 163.0 93 163.0 94 97.0 95 31.0 96 31.0 97 31.0 98 23.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16660906973002926 2 0.2545685294310416 3 0.25531331740480123 4 0.4483251208046093 5 0.2503232379806117 6 0.01858245994530277 7 0.018917614533494605 8 0.017912150768919104 9 0.018470741749238826 10 0.021226457252149458 11 0.019997557095446067 12 0.02845090059761787 13 0.02722200044091448 14 0.02524831231045146 15 0.04062818396858782 16 0.021189217853461475 17 0.04114953555021956 18 0.032063122270352074 19 0.02722200044091448 20 0.029791518950385208 21 0.03537742875358243 22 0.0449107148177057 23 0.02949360376088135 24 0.04900704867338366 25 0.037276638086669485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2685328.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.44256556301022 #Duplication Level Percentage of deduplicated Percentage of total 1 80.6931589178258 55.228468197196946 2 12.295530395426727 16.830752904419576 3 3.502378949859017 7.191354027066979 4 1.374482638572672 3.7629247242291752 5 0.6650526211650796 2.275895381347138 6 0.3988581438528243 1.6379324796592492 7 0.23444196506264403 1.1232066695164677 8 0.16349616376516027 0.8952077526238109 9 0.11809237679837618 0.7274290717363112 >10 0.5163771276790295 6.014479855167163 >50 0.024645706844535487 1.1394593985641603 >100 0.011281433265300867 1.4793621693409011 >500 0.0013772249268519934 0.6739647725510712 >1k 8.263349560697788E-4 1.0195625965811144 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3442 0.12817801028403233 No Hit CCCATGTACTCTGCGTTGATACCAC 2875 0.10706327122794682 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.723939868798151E-5 0.0 4 0.0 0.0 0.0 7.447879737596301E-5 0.0 5 0.0 0.0 0.0 7.447879737596301E-5 0.0 6 7.447879737596301E-5 0.0 0.0 7.447879737596301E-5 0.0 7 7.447879737596301E-5 0.0 0.0 7.447879737596301E-5 0.0 8 7.447879737596301E-5 0.0 0.0 7.447879737596301E-5 0.0 9 7.447879737596301E-5 0.0 0.0 7.447879737596301E-5 0.0 10 1.1171819606394452E-4 1.1171819606394452E-4 3.723939868798151E-5 7.447879737596301E-5 0.0 11 1.1171819606394452E-4 1.1171819606394452E-4 3.723939868798151E-5 7.447879737596301E-5 0.0 12 1.1171819606394452E-4 1.1171819606394452E-4 3.723939868798151E-5 7.447879737596301E-5 0.0 13 1.1171819606394452E-4 1.1171819606394452E-4 3.723939868798151E-5 7.447879737596301E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 645 0.0 13.000023 4 GGACCGC 130 2.0190782E-10 12.405942 6 AACCGTC 60 0.005935764 11.068665 7 GTAGGAC 1305 0.0 10.806185 3 TGGCGAG 1355 0.0 10.50523 18 TGTAGGA 1480 0.0 10.501414 2 GTCCTAC 1375 0.0 10.349746 1 GCGAATC 75 0.0026729393 10.122185 15 TACACCG 85 6.52989E-4 10.069895 5 TACCGTA 95 1.6669597E-4 9.986765 7 GTCCTAA 660 0.0 9.985072 1 GTCTAGC 175 2.3483153E-9 9.823824 1 TATGGCG 1530 0.0 9.738185 16 GGCGAGG 655 0.0 9.707228 19 ACTGTTC 540 0.0 9.66312 8 GTATTAC 270 0.0 9.55094 1 GTATAGG 300 0.0 9.55094 1 AGCACCG 150 2.6080306E-7 9.510456 5 AGGACCG 170 1.6365448E-8 9.510456 5 TCCTACA 1585 0.0 9.504948 2 >>END_MODULE