FastQCFastQC Report
Thu 26 May 2016
SRR1513156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685328
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38540.14352064254348074No Hit
CCCATGTACTCTGCGTTGATACCAC35650.13275845632265407No Hit
GTATCAACGCAGAGTACTTTTTTTT34900.12996550142105545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG250.006070800618.97956811
TAGGACC5450.012.2267414
GGACCGA951.3317887E-511.0216286
ATCGTCC700.001505931610.8450648
GTAGGAC14600.010.6940133
GTCCTAC15650.010.649661
TGTAGGA15800.010.5459932
CGACTAC1106.115255E-610.35422916
TCCTACA17050.010.2754292
ACCGAGT856.6641107E-410.0476338
TGGCGAG13600.09.91029718
AGAACCG1252.5054378E-69.9001675
AGGACCT12800.09.8912425
TCTTATA3200.09.8190732
ACCGTGC1554.1372914E-89.7955438
GTCCTAG3600.09.7883891
GGACGTG13050.09.773976
GACGTGG7300.09.6210377
GTCCTAA8000.09.5238381
TACACCG1401.0301974E-69.5193915
Produced by FastQC (version 0.11.5)