##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513156_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2685328 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.750256207062975 31.0 31.0 34.0 27.0 34.0 2 30.841470390209317 33.0 31.0 34.0 27.0 34.0 3 30.945755229901152 33.0 31.0 34.0 27.0 34.0 4 34.33865397448654 37.0 35.0 37.0 31.0 37.0 5 34.09027984663326 37.0 35.0 37.0 30.0 37.0 6 34.017456712922964 37.0 35.0 37.0 30.0 37.0 7 33.879540227488036 37.0 35.0 37.0 29.0 37.0 8 33.84685967598744 37.0 35.0 37.0 29.0 37.0 9 35.399632000262166 39.0 35.0 39.0 30.0 39.0 10 35.239793798001585 39.0 35.0 39.0 29.0 39.0 11 35.259496419059424 39.0 35.0 39.0 29.0 39.0 12 35.1898133114465 39.0 35.0 39.0 28.0 39.0 13 35.16783350115889 39.0 35.0 39.0 28.0 39.0 14 36.33407762478178 40.0 37.0 41.0 28.0 41.0 15 36.31126514153951 40.0 37.0 41.0 28.0 41.0 16 36.273188228775034 40.0 36.0 41.0 28.0 41.0 17 36.20275400249057 40.0 36.0 41.0 27.0 41.0 18 36.15213411546001 40.0 36.0 41.0 27.0 41.0 19 36.22158187007323 40.0 37.0 41.0 27.0 41.0 20 36.18761171819607 40.0 37.0 41.0 27.0 41.0 21 36.131922804216096 40.0 37.0 41.0 27.0 41.0 22 36.08013024852085 40.0 37.0 41.0 27.0 41.0 23 36.003491193627 40.0 37.0 41.0 27.0 41.0 24 35.93667700928899 40.0 36.0 41.0 27.0 41.0 25 35.87792329279701 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5828419343349474 1101 2 0.623129148915929 1101 3 0.5496076287159966 1101 4 0.7554328359634681 1101 5 0.6630440741374741 1101 6 0.675387336361652 1101 7 0.7445482429577055 1101 8 0.7126725908987837 1101 9 0.8459329103486937 1101 10 0.9063438156915566 1101 11 0.8976738466834675 1101 12 0.8540077974101337 1101 13 0.8852427750450858 1101 14 0.9564152171938076 1101 15 0.9748138696602098 1101 16 1.0358455098614172 1101 17 0.9800718305335607 1101 18 1.023053907920108 1101 19 1.0325411037035366 1101 20 1.029677929094575 1101 21 1.0545418420640544 1101 22 1.0755457398828483 1101 23 1.1247805252499603 1101 24 1.1309107172215747 1101 25 1.1347612580702844 1102 1 0.42198689426908587 1102 2 0.4851983773675883 1102 3 0.4537729780384794 1102 4 0.5543387273746276 1102 5 0.5565995024014185 1102 6 0.4543890560382522 1102 7 0.5186216695528074 1102 8 0.49605353460076174 1102 9 0.5147334886163009 1102 10 0.57984963742296 1102 11 0.7190507162273363 1102 12 0.6369029665695294 1102 13 0.619439294280788 1102 14 0.6835554210504995 1102 15 0.6030104905638893 1102 16 0.6666794228261921 1102 17 0.7284279709692711 1102 18 0.7774785725707716 1102 19 0.8359345203302198 1102 20 0.7913396177500402 1102 21 0.8093039328039637 1102 22 0.7406062385270644 1102 23 0.6498222773115288 1102 24 0.7577885681093761 1102 25 0.8528181722984698 1103 1 0.41422057199499207 1103 2 0.3856043010895718 1103 3 0.4066921915794559 1103 4 0.5339488805599117 1103 5 0.4406625102986226 1103 6 0.3396916589574346 1103 7 0.5010383100005313 1103 8 0.4683249312244939 1103 9 0.4753206352138051 1103 10 0.4664317245231828 1103 11 0.5619787866165495 1103 12 0.5735718338083444 1103 13 0.4830141873893581 1103 14 0.612794780859808 1103 15 0.629959376061997 1103 16 0.5939764594496566 1103 17 0.6913937966293986 1103 18 0.6569722712354533 1103 19 0.6711154249411777 1103 20 0.7305440533275345 1103 21 0.5375971678329492 1103 22 0.5858756449363725 1103 23 0.537040226705642 1103 24 0.6865642922218882 1103 25 0.669679707812854 1104 1 0.3235751375005904 1104 2 0.40496494176568376 1104 3 0.3789262681984269 1104 4 0.5005405303427963 1104 5 0.47615377611875687 1104 6 0.4100735567449334 1104 7 0.39899220454395135 1104 8 0.5177561695716904 1104 9 0.46914580796711647 1104 10 0.5636173943032503 1104 11 0.5507666554287809 1104 12 0.4731800900548464 1104 13 0.4475684105306712 1104 14 0.5800262687977522 1104 15 0.509744919550343 1104 16 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2214 12 -0.8587030503749418 2214 13 -0.8786972117266174 2214 14 -0.9884162150076818 2214 15 -0.9624039101944177 2214 16 -1.066271366447026 2214 17 -1.0873978333072714 2214 18 -1.0281081596465 2214 19 -1.0058243878765012 2214 20 -0.9951681960488372 2214 21 -1.0980937625144662 2214 22 -1.1556320668003508 2214 23 -1.1651373151665538 2214 24 -1.0996546123176287 2214 25 -1.0971057452321347 2215 1 -0.4833078272701563 2215 2 -0.4904894468049079 2215 3 -0.5059521629296171 2215 4 -0.3354571716614956 2215 5 -0.56346621956137 2215 6 -0.587327058280664 2215 7 -0.7235449653140051 2215 8 -0.7000737275306577 2215 9 -0.9801924605643677 2215 10 -0.9127479870148463 2215 11 -0.7851477108298752 2215 12 -0.8236538196330301 2215 13 -0.7221405768147662 2215 14 -0.8900095483156392 2215 15 -0.8746043824779974 2215 16 -0.8929915456170718 2215 17 -0.8746005257634621 2215 18 -0.8977390422052949 2215 19 -0.9571323549976469 2215 20 -0.878980536910035 2215 21 -0.8445087123533668 2215 22 -0.9052614036961089 2215 23 -0.99287332903058 2215 24 -1.0136404205513827 2215 25 -1.0283741171605314 2216 1 -0.49372972863823605 2216 2 -0.45737726140418644 2216 3 -0.5068712610083885 2216 4 -0.3632665166554858 2216 5 -0.6783462989540894 2216 6 -0.6807533980729872 2216 7 -1.008140491861198 2216 8 -0.8896922877683338 2216 9 -0.9957985993200609 2216 10 -1.0351183843439955 2216 11 -0.8573036887634871 2216 12 -0.9976872301336499 2216 13 -1.1054898377000342 2216 14 -1.3122426734459438 2216 15 -1.1873055607151315 2216 16 -1.2383540972711415 2216 17 -1.3348042417637629 2216 18 -1.250406726387446 2216 19 -1.180070128683404 2216 20 -1.2331682177018095 2216 21 -1.3748776129345117 2216 22 -1.228608684182106 2216 23 -1.3785028107886106 2216 24 -1.3384751596468334 2216 25 -1.4535919008246054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64656.0 3 1.0 4 1061.0 5 8690.0 6 9678.0 7 7616.0 8 6177.0 9 5370.0 10 4830.0 11 4358.0 12 4116.0 13 3913.0 14 3757.0 15 3792.0 16 3850.0 17 4226.0 18 4544.0 19 5482.0 20 7004.0 21 8985.0 22 11549.0 23 14043.0 24 16263.0 25 18402.0 26 20362.0 27 22427.0 28 25232.0 29 30521.0 30 38912.0 31 49735.0 32 63712.0 33 82688.0 34 109692.0 35 147075.0 36 211033.0 37 345931.0 38 1315645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.5090358602953 18.60525845548585 13.273983370122403 27.611722314096447 2 14.333113354268587 19.674135526966552 38.89361454900816 27.099136569756705 3 17.788026636605334 23.46694463716917 29.23743946860859 29.50758925761691 4 11.773882292831198 16.148966548665054 36.2288913533591 35.84825980514465 5 13.392016929021025 37.57054535195436 35.27750229855885 13.759935420465762 6 33.84024878283506 36.098504433797885 16.8835123091315 13.177734474235555 7 29.116586739935528 30.830473130984725 21.14926866482253 18.90367146425722 8 26.30957546458294 36.2462033531538 19.324080471221837 18.120140711041422 9 26.394731108913877 14.803841061822325 18.991879546923034 39.809548282340764 10 14.985671591766053 28.57093912055787 33.772626130026346 22.67076315764973 11 35.66007578962421 21.951769020395272 23.08663969541151 19.301515494569006 12 23.457022754762175 24.129342858674992 30.698894138816563 21.714740247746274 13 29.281823300542804 20.84155082731048 25.32118981368384 24.555436058462877 14 21.58376072322861 20.72891886617917 26.629915852726633 31.05740455786559 15 23.98016182753094 29.258697633957564 22.618689411498334 24.14245112701316 16 23.447787383887555 27.41140002264155 25.904358797137633 23.236453796333258 17 22.770123898973765 27.716435833561782 26.60695449290218 22.90648577456227 18 22.869934777596036 26.97929265969506 27.688473153543647 22.462299409165258 19 24.132806122752974 26.45490606734075 26.64866265871432 22.763625151191956 20 24.265452860879567 26.36851066238463 26.8002270113744 22.5658094653614 21 23.825620198181888 25.948949674730414 26.482258479557863 23.74317164752984 22 23.941794754824837 26.530233770586424 26.212134471618892 23.315837002969843 23 23.268075065442613 26.41092188708603 26.49490145342374 23.826101594047618 24 23.673234703544598 26.265804400803177 26.81028164902016 23.25067924663207 25 23.572305028255425 26.20975114744025 26.597376481431485 23.620567342872835 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 98.0 1 98.0 2 218.5 3 339.0 4 339.0 5 339.0 6 826.5 7 1314.0 8 1314.0 9 1314.0 10 2060.0 11 2806.0 12 2806.0 13 2806.0 14 5146.5 15 7487.0 16 7487.0 17 7487.0 18 14642.5 19 21798.0 20 21798.0 21 21798.0 22 35702.5 23 49607.0 24 49607.0 25 49607.0 26 72604.5 27 95602.0 28 95602.0 29 95602.0 30 126139.5 31 156677.0 32 156677.0 33 156677.0 34 185482.0 35 214287.0 36 214287.0 37 214287.0 38 243033.5 39 271780.0 40 271780.0 41 271780.0 42 294859.0 43 317938.0 44 317938.0 45 317938.0 46 340880.0 47 363822.0 48 363822.0 49 363822.0 50 362207.5 51 360593.0 52 360593.0 53 360593.0 54 329813.5 55 299034.0 56 299034.0 57 299034.0 58 261803.5 59 224573.0 60 224573.0 61 224573.0 62 186590.0 63 148607.0 64 148607.0 65 148607.0 66 116073.5 67 83540.0 68 83540.0 69 83540.0 70 61561.0 71 39582.0 72 39582.0 73 39582.0 74 28013.0 75 16444.0 76 16444.0 77 16444.0 78 11408.5 79 6373.0 80 6373.0 81 6373.0 82 4287.0 83 2201.0 84 2201.0 85 2201.0 86 1423.0 87 645.0 88 645.0 89 645.0 90 406.0 91 167.0 92 167.0 93 167.0 94 90.0 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17617959519284052 2 0.008341625306107858 3 0.005623149201885207 4 0.0020109275291510013 5 0.0791337222119607 6 0.32085465909564864 7 0.02927016736875346 8 0.01463508368437673 9 0.00495284002550154 10 0.0038356580648620953 11 0.0 12 0.0 13 0.0 14 0.0013406183527673342 15 0.0 16 0.0 17 0.020854063265269642 18 0.030573546322832816 19 0.0 20 0.0 21 6.330697776956855E-4 22 1.1171819606394452E-4 23 0.005623149201885207 24 0.0 25 1.1171819606394452E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2685328.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.4523566430799 #Duplication Level Percentage of deduplicated Percentage of total 1 78.8710672329535 51.62297221351605 2 13.248820733345234 17.343330794782872 3 3.882967298952921 7.624480814534499 4 1.5296701077757024 4.00482053761575 5 0.7653907827701766 2.504831524259985 6 0.43740941177202114 1.717768609100527 7 0.2761970568428021 1.2654423787770865 8 0.1843515912638756 0.9652996879297984 9 0.13364366180453272 0.7872563353965686 >10 0.6229915557968965 6.940535794561507 >50 0.030510461358536512 1.343001533213852 >100 0.014244968268232688 1.7435675364623526 >500 0.0016524786619322072 0.7597583805169026 >1k 0.0010826584335840927 1.3769338593322598 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3854 0.14352064254348074 No Hit CCCATGTACTCTGCGTTGATACCAC 3565 0.13275845632265407 No Hit GTATCAACGCAGAGTACTTTTTTTT 3490 0.12996550142105545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.447879737596301E-5 0.0 0.0 0.0 0.0 7 7.447879737596301E-5 0.0 0.0 0.0 0.0 8 7.447879737596301E-5 0.0 0.0 0.0 0.0 9 7.447879737596301E-5 0.0 0.0 0.0 0.0 10 7.447879737596301E-5 7.447879737596301E-5 0.0 0.0 0.0 11 1.1171819606394452E-4 7.447879737596301E-5 0.0 0.0 0.0 12 1.1171819606394452E-4 7.447879737596301E-5 0.0 0.0 0.0 13 1.4895759475192603E-4 7.447879737596301E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAACG 25 0.0060708006 18.979568 11 TAGGACC 545 0.0 12.226741 4 GGACCGA 95 1.3317887E-5 11.021628 6 ATCGTCC 70 0.0015059316 10.845064 8 GTAGGAC 1460 0.0 10.694013 3 GTCCTAC 1565 0.0 10.64966 1 TGTAGGA 1580 0.0 10.545993 2 CGACTAC 110 6.115255E-6 10.354229 16 TCCTACA 1705 0.0 10.275429 2 ACCGAGT 85 6.6641107E-4 10.047633 8 TGGCGAG 1360 0.0 9.910297 18 AGAACCG 125 2.5054378E-6 9.900167 5 AGGACCT 1280 0.0 9.891242 5 TCTTATA 320 0.0 9.819073 2 ACCGTGC 155 4.1372914E-8 9.795543 8 GTCCTAG 360 0.0 9.788389 1 GGACGTG 1305 0.0 9.77397 6 GACGTGG 730 0.0 9.621037 7 GTCCTAA 800 0.0 9.523838 1 TACACCG 140 1.0301974E-6 9.519391 5 >>END_MODULE