FastQCFastQC Report
Thu 26 May 2016
SRR1513155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137970
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC28990.1355959157518581No Hit
CCCATGTACTCTGCGTTGATACCAC24300.11365921879165752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC551.9084242E-413.8622633
TAGGACC4450.012.4212294
TAGGACT3550.011.8119334
TTAGGAC5800.011.3377773
CTTCGCA600.00592857911.0702989
ATAGGAC2800.010.8917773
TGTACCG803.732306E-410.6997175
GTCCTAA5400.010.6131731
GACGTGA3800.010.4854437
GTAGGAC9400.010.4427813
TGGCGAG8000.010.43868618
AGGACGT8000.010.1052885
GTCCTAC9400.010.0599351
GGACGTG7550.010.0517996
TCTACAC3100.09.8377353
TAGGACG8650.09.6953434
TAGGACA3750.09.6571494
GGCGAGG4350.09.59901719
GTATAGG2800.09.5518551
TCTAGGA3200.09.5370292
Produced by FastQC (version 0.11.5)