##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513155_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2137970
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.596101442022103	31.0	30.0	34.0	25.0	34.0
2	29.461494314700392	31.0	30.0	34.0	23.0	34.0
3	29.640882706492608	31.0	30.0	34.0	25.0	34.0
4	32.71264049542323	37.0	33.0	37.0	24.0	37.0
5	32.87433967735749	37.0	35.0	37.0	25.0	37.0
6	33.21896518660225	37.0	35.0	37.0	27.0	37.0
7	33.197001828837635	37.0	35.0	37.0	27.0	37.0
8	33.06567070632423	37.0	35.0	37.0	26.0	37.0
9	34.31444547865499	39.0	35.0	39.0	25.0	39.0
10	33.908701712372014	39.0	34.0	39.0	22.0	39.0
11	34.37459459206631	39.0	35.0	39.0	27.0	39.0
12	34.36217954414702	39.0	35.0	39.0	27.0	39.0
13	34.407655860465766	39.0	35.0	39.0	27.0	39.0
14	34.643351403434096	40.0	34.0	41.0	17.0	41.0
15	35.0784669569732	40.0	35.0	41.0	24.0	41.0
16	34.92448397311468	40.0	34.0	41.0	22.0	41.0
17	34.53046020290275	39.0	34.0	41.0	17.0	41.0
18	34.261770745146094	39.0	33.0	41.0	17.0	41.0
19	34.903387325360036	40.0	34.0	41.0	22.0	41.0
20	35.122279077816806	40.0	35.0	41.0	25.0	41.0
21	35.166235728284306	40.0	36.0	41.0	25.0	41.0
22	34.85632819917959	40.0	35.0	41.0	19.0	41.0
23	34.40137887809464	40.0	34.0	41.0	16.0	41.0
24	34.6601570648793	40.0	34.0	41.0	18.0	41.0
25	34.310226991024194	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6134174461604651
1101	2	0.896481748812672
1101	3	0.7046321290374564
1101	4	1.1103118238870664
1101	5	0.9006530158486399
1101	6	0.6732293240890428
1101	7	0.5590388162671687
1101	8	0.7279306373484786
1101	9	1.059318224705322
1101	10	1.340998988550112
1101	11	1.0115145277537678
1101	12	0.7755071262716129
1101	13	0.7145680045687826
1101	14	1.631878795387756
1101	15	1.1904886643436612
1101	16	1.3112490196965823
1101	17	1.6601372053664676
1101	18	1.662362180177034
1101	19	1.1912846911963157
1101	20	0.8259959345098622
1101	21	0.8089233427702567
1101	22	0.9990346780381572
1101	23	1.301684306662743
1101	24	1.0076024929139322
1101	25	1.27426889063765
1102	1	0.9201777096510533
1102	2	1.1844439108549452
1102	3	1.0487320207619426
1102	4	1.2540103703336314
1102	5	1.1842027993984203
1102	6	0.9773207070113585
1102	7	0.9329538935823436
1102	8	0.9230991701043365
1102	9	1.311262042415322
1102	10	1.6484592119162542
1102	11	1.1798088556894584
1102	12	1.125671656664366
1102	13	1.1046549475900491
1102	14	2.1037350248592617
1102	15	1.6163674199615912
1102	16	1.7126593234124314
1102	17	2.0492415027148283
1102	18	2.348928253445486
1102	19	1.6784650314274216
1102	20	1.4590111959135683
1102	21	1.3071030236700096
1102	22	1.5285175767572028
1102	23	1.8214238203705833
1102	24	1.5360096935381833
1102	25	1.8361130626122346
1103	1	0.801241112976232
1103	2	1.1238130610403587
1103	3	0.841909178644908
1103	4	1.2748545223793712
1103	5	1.1385847410324885
1103	6	0.8282671031017799
1103	7	0.7942403921873975
1103	8	0.816688801967679
1103	9	1.098612614857025
1103	10	1.4253962638225914
1103	11	0.9836015828883902
1103	12	1.0289197375491383
1103	13	0.9737897132313762
1103	14	1.7898572680523799
1103	15	1.4302309507834252
1103	16	1.5350299932911824
1103	17	1.7518881726206388
1103	18	1.9572553492315308
1103	19	1.49214780270502
1103	20	1.207250556170024
1103	21	1.1836001842429837
1103	22	1.4648061545446893
1103	23	1.7854104905231125
1103	24	1.4265075114909394
1103	25	1.6430004887117207
1104	1	0.8575319498517473
1104	2	1.0493155671917975
1104	3	0.8907088484836834
1104	4	1.2726393859975929
1104	5	1.1927492357799068
1104	6	0.9588144208515672
1104	7	0.9073937711332505
1104	8	0.8733687405727935
1104	9	1.2617648285626686
1104	10	1.3133036959606272
1104	11	1.094547790385512
1104	12	1.0294014685001898
1104	13	1.0458493475701474
1104	14	1.940035785801939
1104	15	1.5968024807497159
1104	16	1.7905400307293178
1104	17	2.0889505696111925
1104	18	2.125326056597231
1104	19	1.461479860469069
1104	20	1.259284873821585
1104	21	1.2945676315615842
1104	22	1.5155837827587249
1104	23	1.82494841820683
1104	24	1.6099800394043413
1104	25	1.856366590369639
1105	1	0.6033219934228278
1105	2	0.8176426485431065
1105	3	0.7743686698398875
1105	4	1.1051453842018688
1105	5	1.0749908526549916
1105	6	0.7221734585263562
1105	7	0.6125626533386637
1105	8	0.7445536151042944
1105	9	0.9139299658741322
1105	10	1.2942145928229891
1105	11	0.8225087668435975
1105	12	0.7837667408450244
1105	13	0.6892774482030575
1105	14	1.6385568713883885
1105	15	1.2584845786772405
1105	16	1.415528423840101
1105	17	1.7573334820669189
1105	18	1.3871200331332147
1105	19	1.272507900352707
1105	20	1.0955854395295646
1105	21	1.0165241084811925
1105	22	1.2237332747704315
1105	23	1.3966279429460329
1105	24	1.2086588606480149
1105	25	1.3911115181590503
1106	1	0.6183710065319836
1106	2	0.8080400563462113
1106	3	0.7403484251252692
1106	4	1.175102141928157
1106	5	1.0654058069172194
1106	6	0.7299794882695494
1106	7	0.6937606475834315
1106	8	0.6651011036211116
1106	9	0.851634577083594
1106	10	0.909157352374109
1106	11	0.6707320903769087
1106	12	0.7546515112983485
1106	13	0.6537836908432908
1106	14	1.6784310047882656
1106	15	1.2793712987371322
1106	16	1.3184541984187916
1106	17	1.644891872783674
1106	18	1.738598224834135
1106	19	1.2133693145193263
1106	20	0.9658777245464449
1106	21	0.9181455962649565
1106	22	1.1453992525761123
1106	23	1.6002676634504311
1106	24	1.2748133006820197
1106	25	1.5151263819595329
1107	1	0.671740579575399
1107	2	0.9286318916115306
1107	3	0.8949347884343624
1107	4	1.1789721109684024
1107	5	1.0246469329882117
1107	6	0.7204827642433429
1107	7	0.7491894041533769
1107	8	0.827161007141882
1107	9	1.0690044381247006
1107	10	1.213524117439988
1107	11	0.8539814243822335
1107	12	0.8600514680986961
1107	13	0.7720139450012553
1107	14	1.7021080153721826
1107	15	1.166292603366692
1107	16	1.26265104484402
1107	17	1.6803515891059462
1107	18	1.880519889460814
1107	19	1.356421370102879
1107	20	1.1598225729876575
1107	21	0.9980613569388694
1107	22	1.2946468585952644
1107	23	1.6420129294883026
1107	24	1.4123538003580194
1107	25	1.7503880998657948
1108	1	0.5471522113389362
1108	2	0.797886223597061
1108	3	0.5032112125258159
1108	4	1.049297013159645
1108	5	0.9188311261038393
1108	6	0.6247535502667176
1108	7	0.4781748888800834
1108	8	0.5824656511531856
1108	9	0.7953983510480427
1108	10	1.0476767204504327
1108	11	0.6743425145984361
1108	12	0.5537727240796428
1108	13	0.5749545168881269
1108	14	1.5524040719820107
1108	15	0.9985747741090236
1108	16	1.1500200762068502
1108	17	1.3866156557263594
1108	18	1.5445842820542737
1108	19	1.0183094264820625
1108	20	0.8994052705812052
1108	21	0.7994743748179616
1108	22	0.9470841866647035
1108	23	1.2424769150086021
1108	24	1.0070009306083278
1108	25	1.3459500401729585
1109	1	0.35188143089571966
1109	2	0.46696460247250116
1109	3	0.5399911252646348
1109	4	0.7378447457275072
1109	5	0.7783331403565583
1109	6	0.5508665646774702
1109	7	0.4556090663912471
1109	8	0.47393351876239365
1109	9	0.6954187754504844
1109	10	1.0393501259376592
1109	11	0.5829654015738583
1109	12	0.552662043226178
1109	13	0.4394262226214636
1109	14	1.3301889762229848
1109	15	0.9610592132096443
1109	16	1.0702018811140661
1109	17	1.3832651295438438
1109	18	1.4311884617557453
1109	19	0.7932510875497343
1109	20	0.5931232419297388
1109	21	0.5903285861726388
1109	22	0.7781333447819208
1109	23	1.1264553113510658
1109	24	0.8579313499370187
1109	25	1.1097425652113415
1110	1	0.36422110200667035
1110	2	0.5521808768723417
1110	3	0.46793041285260273
1110	4	0.8417360567610288
1110	5	0.7519957848560779
1110	6	0.3227002387191149
1110	7	0.4389665206669804
1110	8	0.37462910643900216
1110	9	0.46507704199895983
1110	10	0.7742516220391167
1110	11	0.3254631854622758
1110	12	0.2632803835847355
1110	13	0.14642008987186017
1110	14	0.9950667734985217
1110	15	0.684133909738037
1110	16	0.8573439112531815
1110	17	1.1060883652476647
1110	18	0.750731852316008
1110	19	0.4807531227542512
1110	20	0.33230771286702065
1110	21	0.23098925878511523
1110	22	0.40838010202272557
1110	23	-0.617266308852642
1110	24	-0.01012950720066641
1110	25	0.40255060472144066
1111	1	0.201479937398922
1111	2	0.5269620632418324
1111	3	0.43283800518495497
1111	4	0.8395887680298202
1111	5	0.7094004487452281
1111	6	0.2803505196901739
1111	7	0.24369783932590394
1111	8	0.29537285126659896
1111	9	0.5012532837740693
1111	10	0.8029823523512079
1111	11	0.41906128077800986
1111	12	0.22228917866105036
1111	13	0.14733592614840063
1111	14	1.0213080169660103
1111	15	0.6137848180427667
1111	16	0.7633433654374429
1111	17	1.07670345350855
1111	18	1.31459548344381
1111	19	0.8379360933032345
1111	20	0.33982791956815106
1111	21	0.3398566379657879
1111	22	0.6429026816046104
1111	23	0.9426229685263081
1111	24	0.6290045951185164
1111	25	0.8379909699217905
1112	1	0.09963808605712643
1112	2	0.24837029988205472
1112	3	0.2027052212125362
1112	4	0.6033132316539849
1112	5	0.4489011360811972
1112	6	0.11511537850837783
1112	7	-0.002109871893601678
1112	8	0.18562683561399496
1112	9	0.23077163306226822
1112	10	0.5489550436902775
1112	11	0.01456492810091703
1112	12	-0.05896698041220105
1112	13	0.023534224368503942
1112	14	0.43126098190899853
1112	15	0.3103573682385772
1112	16	0.21658206469786734
1112	17	0.5683306570782491
1112	18	0.8444032942271633
1112	19	0.3426470840086324
1112	20	0.1944817186417609
1112	21	0.0033886085441423575
1112	22	0.41697150945031325
1112	23	0.686288329849603
1112	24	0.413194034282661
1112	25	0.6067528855832407
1113	1	0.07796862183910491
1113	2	0.16857438373131828
1113	3	0.05371802857891339
1113	4	0.4694711873327222
1113	5	0.4233036747277339
1113	6	0.12106359528973343
1113	7	0.1474742523778616
1113	8	0.22916661084720147
1113	9	0.2875291039666408
1113	10	0.5351828826588658
1113	11	0.19291002883679909
1113	12	0.0928979479800347
1113	13	-0.0018121784885067882
1113	14	0.6081383697468326
1113	15	0.32324058633131614
1113	16	0.6106820297811453
1113	17	0.8270083337579948
1113	18	0.580606427971361
1113	19	0.4212438842413846
1113	20	0.3642868242456885
1113	21	0.21965582808554274
1113	22	0.33416404870402516
1113	23	0.3675263901039969
1113	24	0.31947398658510906
1113	25	0.7229777456804527
1114	1	-1.1372887379727956
1114	2	-0.8544706468564769
1114	3	-0.7894818325333155
1114	4	-0.506667781481454
1114	5	-3.6015534291058984
1114	6	-1.4875484945835105
1114	7	-1.1097683642314564
1114	8	-0.7032143258977328
1114	9	-0.5330875214167818
1114	10	-0.34795839128713624
1114	11	-0.7150333317792246
1114	12	-0.7782572692364553
1114	13	-0.816313515143591
1114	14	-6.210910877775557
1114	15	-2.5243129663277983
1114	16	-1.3719079197881996
1114	17	-0.38915567397864237
1114	18	-6.1608609339259495
1114	19	-2.4464959023898274
1114	20	-1.4634816830915014
1114	21	-1.2133175474497406
1114	22	-0.9490899842287277
1114	23	-0.5131502895700066
1114	24	-0.6318614982977451
1114	25	-0.5428903395790385
1115	1	-0.6401394251019177
1115	2	-0.33848200313361687
1115	3	-0.34615616808388694
1115	4	-3.0536823771456056
1115	5	-1.0333535278243815
1115	6	-0.6202397151774974
1115	7	-0.4423147269000651
1115	8	-0.34142634625087
1115	9	-0.14805057501122576
1115	10	-0.4269190721857754
1115	11	-0.4559934039028022
1115	12	-0.3982322609960889
1115	13	-0.5548834646922742
1115	14	-5.774769190525944
1115	15	-2.261572754942776
1115	16	-1.1411001841625463
1115	17	-0.2343309656778203
1115	18	-0.11434872705661547
1115	19	-0.43463142753466144
1115	20	-0.3594322667554195
1115	21	-0.6757170572430908
1115	22	-1.5756869814474186
1115	23	-0.826464306300366
1115	24	-0.5256347319123549
1115	25	-0.1137059155901099
1116	1	0.014870800215994961
1116	2	0.18850790358507652
1116	3	0.12045075126595961
1116	4	0.4102101221975971
1116	5	-0.3195215953874282
1116	6	-0.15101259100850228
1116	7	-0.05254168399313386
1116	8	-0.047108025840728374
1116	9	-0.1268946411311873
1116	10	0.2166701369645736
1116	11	-0.1102754728953883
1116	12	-0.37314357615738913
1116	13	-0.3253390255764117
1116	14	0.5689194671535134
1116	15	0.08266247113081704
1116	16	0.0986173898347289
1116	17	0.497223572885062
1116	18	0.461741277997767
1116	19	0.137645005389615
1116	20	-0.26538182425984047
1116	21	-0.321915944376002
1116	22	-0.20323712640947633
1116	23	-0.7617823844428813
1116	24	-0.5518331709027962
1116	25	-0.014452659301191773
1201	1	0.8550373365388602
1201	2	1.1035400155585826
1201	3	1.02873327388086
1201	4	1.35482894250773
1201	5	1.2696123710062395
1201	6	1.0803020989885397
1201	7	0.9473437436363525
1201	8	1.0168503102046174
1201	9	1.2965652575377717
1201	10	1.6233143497831222
1201	11	1.1909223549526544
1201	12	1.1919263538775269
1201	13	1.0568262537047985
1201	14	1.9673449273539205
1201	15	1.66427463205752
1201	16	1.8530685222570185
1201	17	2.1360671780110962
1201	18	1.519754534680267
1201	19	1.5314638718958662
1201	20	1.4431766900944965
1201	21	1.2871038802323085
1201	22	1.622309958745916
1201	23	1.8125457107718503
1201	24	1.5835841946302978
1201	25	1.9309531471898325
1202	1	0.8656094789799091
1202	2	1.0242909098222164
1202	3	0.9453687313902854
1202	4	1.3255159760440236
1202	5	1.1984531639426308
1202	6	0.8754032768124347
1202	7	0.7841803614666674
1202	8	0.9805229482018092
1202	9	1.2167435203629893
1202	10	1.4103784096022522
1202	11	0.9716386662359611
1202	12	0.9732226703690401
1202	13	0.9939608882627056
1202	14	1.8912130827976483
1202	15	1.525227163996611
1202	16	1.5687668124350438
1202	17	1.9840313965991285
1202	18	2.037671922068249
1202	19	1.5707975004387222
1202	20	1.3623111764008229
1202	21	1.2081849447407649
1202	22	1.5119011453057851
1202	23	1.784268758964096
1202	24	1.4325977200645639
1202	25	1.881467778107428
1203	1	0.4382592885122101
1203	2	0.5472685659961236
1203	3	0.4860631140440361
1203	4	0.7993321352877132
1203	5	0.7484283022751868
1203	6	0.4644796521058936
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2211	17	-2.127236057335743
2211	18	-0.8364331034866694
2211	19	-0.8214032787284964
2211	20	-0.8288628032436165
2211	21	-0.8562951010666069
2211	22	-0.6910014919322194
2211	23	-0.32395649989911846
2211	24	-0.7579036018684775
2211	25	-7.65137903599712
2212	1	-1.6056167309778822
2212	2	-3.989914171784669
2212	3	-1.8071626536096304
2212	4	-3.513524701998268
2212	5	-1.2966836728375313
2212	6	-1.0562646688360147
2212	7	-0.9769611395834517
2212	8	-0.9248066241758934
2212	9	-1.0070220124872193
2212	10	-0.47420026311790764
2212	11	-0.9669295377418337
2212	12	-1.1264084049282062
2212	13	-1.412084699856642
2212	14	-6.907591577619311
2212	15	-5.499196660954485
2212	16	-3.079946214377909
2212	17	-7.672749115500185
2212	18	-3.3091283580590876
2212	19	-2.207185532806605
2212	20	-2.1253078518512964
2212	21	-1.6899919621639867
2212	22	-1.613217878486843
2212	23	-1.0709761901281496
2212	24	-1.3836975959366953
2212	25	-1.1880788629867425
2213	1	-0.34186683679462604
2213	2	-0.3344614348345196
2213	3	-0.29307017770939225
2213	4	0.09045810807125321
2213	5	-0.13837256102097228
2213	6	-0.4634438721417311
2213	7	-0.46905001506888766
2213	8	-0.5011090960550888
2213	9	-0.31603733475212437
2213	10	-3.9324423295073316
2213	11	-1.6728853010509326
2213	12	-1.158454014591392
2213	13	-0.9513804100457364
2213	14	0.0042634031749528845
2213	15	-0.6041513317056797
2213	16	-0.43056570529353877
2213	17	-0.03639398656248005
2213	18	0.12187019489690698
2213	19	-0.4094466382741544
2213	20	-0.6982380762886109
2213	21	-0.8416134715340604
2213	22	-1.01760153561942
2213	23	-1.3110578542023035
2213	24	-0.7905352095574258
2213	25	-0.46657399012556056
2214	1	-1.122829732422172
2214	2	-0.9143445216348205
2214	3	-0.7573587418683445
2214	4	-0.4042989394209897
2214	5	-3.407532503072467
2214	6	-1.4793231215470044
2214	7	-1.2231059546606922
2214	8	-0.9885183560912054
2214	9	-0.8974339485164577
2214	10	-4.310829933112441
2214	11	-1.9598833325213505
2214	12	-1.5304566807699373
2214	13	-1.1573752731085136
2214	14	-0.5162090276398956
2214	15	-0.6991446906867864
2214	16	-0.6851566882294762
2214	17	-0.39075316895176115
2214	18	-0.09133346323295655
2214	19	-0.8190542774977914
2214	20	-1.2028450999396298
2214	21	-1.2333370162422241
2214	22	-1.0007637517069057
2214	23	-0.8314484529427588
2214	24	-1.2110283060244953
2214	25	-0.8963964316233657
2215	1	-2.194683124863392
2215	2	-4.6704342796942555
2215	3	-2.760452518509233
2215	4	-1.6809831809620093
2215	5	-1.8617141376034603
2215	6	-1.9404932229697849
2215	7	-2.0562461658187523
2215	8	-2.1730707000036205
2215	9	-7.975590823279731
2215	10	-3.6660349200994844
2215	11	-2.8521131169371294
2215	12	-3.3856281072552825
2215	13	-2.701771835780619
2215	14	-7.963794729735547
2215	15	-4.577919779351401
2215	16	-9.309538555940268
2215	17	-4.367197359350239
2215	18	-2.9447889647546432
2215	19	-3.3957780033870755
2215	20	-3.177980709447308
2215	21	-3.2738961764000223
2215	22	-9.419827486165069
2215	23	-10.30160528439189
2215	24	-6.466384868639274
2215	25	-4.733014513989833
2216	1	-1.9724133788979046
2216	2	-1.9321904126170928
2216	3	-1.786794557303292
2216	4	-6.735069071624636
2216	5	-3.554248754392521
2216	6	-2.7057575970790886
2216	7	-2.290793894366125
2216	8	-8.30991068623647
2216	9	-4.048652847233797
2216	10	-2.2107240706161946
2216	11	-2.2902111313237157
2216	12	-2.4191445580872397
2216	13	-2.168597016481975
2216	14	-1.4577419329794878
2216	15	-2.104627423821455
2216	16	-1.8037203311865753
2216	17	-1.569672779485849
2216	18	-7.406556350540701
2216	19	-4.811533015818419
2216	20	-4.169726487038886
2216	21	-3.2995656655724765
2216	22	-3.0806726316334228
2216	23	-2.2854193667133984
2216	24	-2.8000202761704784
2216	25	-2.1331501477020893
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91483.0
3	3.0
4	735.0
5	4518.0
6	5552.0
7	5041.0
8	4697.0
9	4528.0
10	4124.0
11	4034.0
12	3991.0
13	3789.0
14	3943.0
15	3860.0
16	3972.0
17	4229.0
18	4905.0
19	6081.0
20	7703.0
21	9864.0
22	12249.0
23	14539.0
24	16962.0
25	19472.0
26	21974.0
27	24909.0
28	27621.0
29	33054.0
30	40130.0
31	48007.0
32	60845.0
33	79955.0
34	105325.0
35	140836.0
36	201598.0
37	296011.0
38	817431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.455590839963605	18.612004673908665	12.227265529173978	25.70513895695375
2	14.485072736993162	20.442424754026092	40.94187606104092	24.13062644793982
3	18.170792732770956	24.9706297130224	29.926083019073218	26.93249453513343
4	11.36371753445103	16.967707857522836	39.270226528091854	32.39834807993429
5	12.638580411342302	37.91536605863241	36.42999977489476	13.016053755130525
6	31.53823950313604	37.38839531111773	18.393341588825052	12.680023596921172
7	27.7594184050113	31.668109114555314	22.72641363791595	17.846058842517436
8	25.203568852258083	36.08167267494571	21.09111782058995	17.623640652206255
9	26.463437397809553	14.917899111741322	20.237533291572184	38.38113019887694
10	13.582936685788713	28.902296048931973	36.03259511821765	21.48217214706166
11	34.341794092673034	22.476988465578017	24.433044914304695	18.74817252744425
12	22.554820272599503	25.006643747298053	31.92273789761892	20.515798082483528
13	28.61139754801691	21.659495866887	25.894416133457348	23.83469045163874
14	20.45127471914633	21.48205399801255	28.975649055961135	29.091022226879982
15	23.437392522992766	29.64903127390815	23.58142048631664	23.33215571678244
16	22.372813163010143	27.92924460995413	27.391748791230896	22.30619343580483
17	21.7754886601643	27.956901698963254	28.147302753117824	22.120306887754623
18	21.69750892843357	27.447733630659254	29.173484154808488	21.681273286098687
19	23.24012194460092	27.270179529551353	27.588188067170588	21.901510458677137
20	23.52409724519363	27.164733013649347	27.607250017428374	21.703919723728653
21	23.378364341281138	26.74478779184295	27.147418531027135	22.72942933584878
22	23.07131496158423	27.41326327916583	27.202408221194723	22.31301353805522
23	22.386193073142614	27.20132465947755	27.611841834385746	22.80064043299409
24	22.821882396764682	27.290709874145612	27.571207825864775	22.31619990322493
25	22.804548285409073	26.872101581680706	27.483895661385667	22.839454471524554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	203.0
2	318.5
3	434.0
4	434.0
5	434.0
6	900.0
7	1366.0
8	1366.0
9	1366.0
10	2179.0
11	2992.0
12	2992.0
13	2992.0
14	5844.0
15	8696.0
16	8696.0
17	8696.0
18	16309.0
19	23922.0
20	23922.0
21	23922.0
22	38593.5
23	53265.0
24	53265.0
25	53265.0
26	75333.0
27	97401.0
28	97401.0
29	97401.0
30	124434.5
31	151468.0
32	151468.0
33	151468.0
34	176433.5
35	201399.0
36	201399.0
37	201399.0
38	222649.0
39	243899.0
40	243899.0
41	243899.0
42	257797.5
43	271696.0
44	271696.0
45	271696.0
46	279704.0
47	287712.0
48	287712.0
49	287712.0
50	276466.5
51	265221.0
52	265221.0
53	265221.0
54	235901.0
55	206581.0
56	206581.0
57	206581.0
58	176222.0
59	145863.0
60	145863.0
61	145863.0
62	118213.0
63	90563.0
64	90563.0
65	90563.0
66	69666.5
67	48770.0
68	48770.0
69	48770.0
70	35482.0
71	22194.0
72	22194.0
73	22194.0
74	15546.0
75	8898.0
76	8898.0
77	8898.0
78	6209.0
79	3520.0
80	3520.0
81	3520.0
82	2414.5
83	1309.0
84	1309.0
85	1309.0
86	875.0
87	441.0
88	441.0
89	441.0
90	281.0
91	121.0
92	121.0
93	121.0
94	71.5
95	22.0
96	22.0
97	22.0
98	18.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16688728092536376
2	0.26333391020453983
3	0.26721609751306147
4	0.4687624241687208
5	0.2635210035688059
6	0.01884965644980987
7	0.01870933642661029
8	0.017025496148215365
9	0.01950448322474123
10	0.022077016983400144
11	0.021281870185269204
12	0.029093018143378998
13	0.027970457957782382
14	0.026099524315121355
15	0.04139440684387526
16	0.02174960359593446
17	0.0421895536420062
18	0.03260101872336843
19	0.02834464468631459
20	0.03044944503430825
21	0.03629611266762396
22	0.04476208740066512
23	0.03115104515030613
24	0.04939264816625116
25	0.038026726287085415
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2137970.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.57595656099923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.99046547147483	61.890933479778134
2	11.734830075931582	17.502723559867615
3	2.9453482549936827	6.589564905642713
4	1.0533868281236471	3.1422932134431147
5	0.4563830122092458	1.7017599846847347
6	0.2392414303480639	1.0704995110337119
7	0.14033973738189265	0.7326179111141846
8	0.09301635189811289	0.5549426734893027
9	0.060332078898373845	0.40493902456439157
>10	0.26476016646899303	3.340243217379773
>50	0.012385821911458096	0.6230759336605514
>100	0.007737092818558369	1.1323146235487442
>500	0.0011402212766552055	0.5500223859927843
>1k	6.334562648079047E-4	0.7640695758001576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2899	0.1355959157518581	No Hit
CCCATGTACTCTGCGTTGATACCAC	2430	0.11365921879165752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.6773341066525724E-5	0.0	0.0	0.0	0.0
9	4.6773341066525724E-5	0.0	0.0	0.0	0.0
10	4.6773341066525724E-5	0.0	0.0	4.6773341066525724E-5	0.0
11	4.6773341066525724E-5	0.0	0.0	4.6773341066525724E-5	0.0
12	4.6773341066525724E-5	0.0	0.0	4.6773341066525724E-5	0.0
13	4.6773341066525724E-5	0.0	0.0	9.354668213305145E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAC	55	1.9084242E-4	13.862263	3
TAGGACC	445	0.0	12.421229	4
TAGGACT	355	0.0	11.811933	4
TTAGGAC	580	0.0	11.337777	3
CTTCGCA	60	0.005928579	11.070298	9
ATAGGAC	280	0.0	10.891777	3
TGTACCG	80	3.732306E-4	10.699717	5
GTCCTAA	540	0.0	10.613173	1
GACGTGA	380	0.0	10.485443	7
GTAGGAC	940	0.0	10.442781	3
TGGCGAG	800	0.0	10.438686	18
AGGACGT	800	0.0	10.105288	5
GTCCTAC	940	0.0	10.059935	1
GGACGTG	755	0.0	10.051799	6
TCTACAC	310	0.0	9.837735	3
TAGGACG	865	0.0	9.695343	4
TAGGACA	375	0.0	9.657149	4
GGCGAGG	435	0.0	9.599017	19
GTATAGG	280	0.0	9.551855	1
TCTAGGA	320	0.0	9.537029	2
>>END_MODULE