FastQCFastQC Report
Thu 26 May 2016
SRR1513155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137970
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31740.14845858454515262No Hit
CCCATGTACTCTGCGTTGATACCAC31430.14700861097209034No Hit
GTATCAACGCAGAGTACTTTTTTTT27470.12848636790974616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAT500.001513348213.28421710
TATACCG604.031084E-412.6896255
CGAAATC550.003086215112.08193113
TTGCGCG550.00308675612.08164818
GCGTATT658.0820936E-411.68253111
GAACCGC600.005800215511.10290056
GAACCGA600.005800215511.10290056
GTCCTAA5500.010.9069151
GTCCTAC9000.010.8972971
TTAGGAC7000.010.6053983
ATAGGAC3250.010.54264453
GACGTGA5150.010.505287
TCCTACA10900.010.479642
TAGGACT3000.010.4689424
CAATACT2200.010.38242154
CCTACAC3500.010.3334663
TAGGCTG2500.010.2785975
AGGACGT9050.010.0956135
GGACGTG9050.010.095146
TCTACAC3500.010.0615323
Produced by FastQC (version 0.11.5)