FastQCFastQC Report
Thu 26 May 2016
SRR1513154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959103
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34270.17492699465010264No Hit
CCCATGTACTCTGCGTTGATACCAC30430.15532618754603508No Hit
GTATCAACGCAGAGTACTTTTTTTT19860.10137292424134924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGT604.1358412E-412.6509926
ACAGTCG550.003095750512.0768728
TCCAACG1850.011.79950818
TAGGACC3850.010.885954
TGTCGCC700.001504944810.84564510
GTCTTAA1855.456968E-1210.8365571
TCTTATA1909.094947E-1210.535152
TGGCGAG8550.010.32344418
CGGTTTC1752.401066E-1010.30388913
GACGTGG4250.010.2706787
GTATTAC1605.697075E-910.14314651
CCGGTTT1606.2755134E-910.08357612
GTATTAG1909.094947E-1110.0489391
GTCCTAC9700.010.0385791
ATTACAC2500.09.9064673
TAGGACT2600.09.891564
GTAGGAC9350.09.8820183
CCAACGT1451.6399645E-79.81818219
TGCCGGT1752.6211637E-99.76108110
GCCGGTT1856.6029315E-109.746922511
Produced by FastQC (version 0.11.5)