##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513154_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1959103 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.457944783913863 31.0 30.0 34.0 25.0 34.0 2 29.355477991713556 31.0 30.0 34.0 22.0 34.0 3 29.520845509398946 31.0 30.0 34.0 23.0 34.0 4 32.59475076093498 37.0 33.0 37.0 22.0 37.0 5 32.75783560129304 37.0 33.0 37.0 25.0 37.0 6 33.07457239359033 37.0 35.0 37.0 26.0 37.0 7 33.04581229266659 37.0 35.0 37.0 26.0 37.0 8 32.93335725584617 37.0 35.0 37.0 26.0 37.0 9 34.16329973462345 39.0 35.0 39.0 25.0 39.0 10 33.75604039195489 39.0 34.0 39.0 19.0 39.0 11 34.19462376403895 39.0 35.0 39.0 26.0 39.0 12 34.193513562074074 39.0 35.0 39.0 25.0 39.0 13 34.22929677510575 39.0 35.0 39.0 25.0 39.0 14 34.478360760000875 39.0 34.0 41.0 17.0 41.0 15 34.90158097864175 40.0 34.0 41.0 22.0 41.0 16 34.734660199080906 39.0 34.0 41.0 18.0 41.0 17 34.35965081978844 39.0 34.0 41.0 17.0 41.0 18 34.0883975982886 39.0 33.0 41.0 17.0 41.0 19 34.72125610547276 39.0 34.0 41.0 18.0 41.0 20 34.93671848800191 40.0 35.0 41.0 22.0 41.0 21 34.9847021825805 40.0 35.0 41.0 23.0 41.0 22 34.67285027892867 40.0 35.0 41.0 18.0 41.0 23 34.20508365308001 39.0 34.0 41.0 10.0 41.0 24 34.45655128903381 40.0 34.0 41.0 17.0 41.0 25 34.08893611004628 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0475286138196118 1101 2 1.2735980942219314 1101 3 1.1634086033313729 1101 4 1.5238060072527873 1101 5 1.3954830040780877 1101 6 1.14469078891711 1101 7 1.1333784020331947 1101 8 1.1360481896361918 1101 9 1.5898592401589724 1101 10 1.9186095227441058 1101 11 1.5231878957042113 1101 12 1.3259645150264419 1101 13 1.2850846555003912 1101 14 2.3481911548564156 1101 15 1.9131944393515923 1101 16 1.8628322267053434 1101 17 2.242878405135059 1101 18 2.428312827642685 1101 19 1.912029612747979 1101 20 1.5202129767473167 1101 21 1.4614504544373048 1101 22 1.8180827209940347 1101 23 1.95161826927977 1101 24 1.7404120889068722 1101 25 2.0593136664354503 1102 1 0.9963124226950235 1102 2 1.2174614351886959 1102 3 1.1261324116762736 1102 4 1.3933066217586614 1102 5 1.4131290486938681 1102 6 1.0779253886925275 1102 7 0.9645550702619161 1102 8 1.0318010256471197 1102 9 1.5268498413137124 1102 10 1.5920407643996484 1102 11 1.3441283991569364 1102 12 1.2647747609172129 1102 13 1.2278975893017474 1102 14 2.3922459210347142 1102 15 1.7480572586514995 1102 16 1.8910641297283632 1102 17 2.1387024177258027 1102 18 2.4017458376604566 1102 19 1.7214426049052918 1102 20 1.5415880552183907 1102 21 1.4163090296678575 1102 22 1.6653461804410696 1102 23 1.9722625720587459 1102 24 1.852799024246707 1102 25 2.0799340963243083 1103 1 0.6486825521410573 1103 2 0.8183807937494194 1103 3 0.741394460432975 1103 4 1.105674095231592 1103 5 0.9454143611625696 1103 6 0.6733582034579939 1103 7 0.6724023822865064 1103 8 0.7512682200251675 1103 9 0.9869490702106987 1103 10 1.346248522453557 1103 11 0.8364556847088807 1103 12 0.7268455055911147 1103 13 0.7465076880952424 1103 14 1.6035342421549288 1103 15 1.2396948620058623 1103 16 1.2112046786004456 1103 17 1.660365143973344 1103 18 1.81672661451082 1103 19 1.2240454585524603 1103 20 1.0005086416224174 1103 21 1.0190736364314432 1103 22 1.2561147145616118 1103 23 1.6265293275481199 1103 24 1.2197950751194782 1103 25 1.3643568140379116 1104 1 0.6653040385238178 1104 2 0.8953673497810684 1104 3 0.6711791172733363 1104 4 1.2540701141846355 1104 5 1.071485490151474 1104 6 0.8597982885718238 1104 7 0.8552520112899842 1104 8 0.8093720083676814 1104 9 1.095495107138703 1104 10 1.2844052964193509 1104 11 0.9973227575122934 1104 12 0.7390616713881855 1104 13 0.810556165406318 1104 14 1.7817738628348678 1104 15 1.3656574935387837 1104 16 1.4838195968386998 1104 17 1.9050026167275291 1104 18 1.893017491332202 1104 19 1.3531480388486798 1104 20 1.1023569848500756 1104 21 1.1208507275984516 1104 22 1.4512388065026798 1104 23 1.723867091464136 1104 24 1.328452848233482 1104 25 1.651875396251448 1105 1 0.43746508685119423 1105 2 0.7803234011671378 1105 3 0.7110564475157766 1105 4 0.9524699338826323 1105 5 0.9193749907793105 1105 6 0.6260576658859662 1105 7 0.5000699384608538 1105 8 0.6671362634950171 1105 9 0.8562745913461782 1105 10 1.2672041630924582 1105 11 0.9175149723292506 1105 12 0.7638354889611918 1105 13 0.6455814398839266 1105 14 1.471977990462932 1105 15 1.1509161947204092 1105 16 1.3927976708269156 1105 17 1.651713714959925 1105 18 1.2313783529182132 1105 19 1.1654520409092726 1105 20 1.0984724425273953 1105 21 0.9427558114710806 1105 22 1.237786988994067 1105 23 1.4743597023015766 1105 24 1.295016485182515 1105 25 1.4679399830056923 1106 1 0.4922803462883003 1106 2 0.6639787680758076 1106 3 0.547975024470368 1106 4 1.042502137240163 1106 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2216 23 -1.25994555327798 2216 24 -1.7143074747830411 2216 25 -0.8546782075390595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90428.0 3 1.0 4 752.0 5 4468.0 6 5386.0 7 4929.0 8 4542.0 9 4166.0 10 4000.0 11 3736.0 12 3777.0 13 3690.0 14 3654.0 15 3700.0 16 3640.0 17 4070.0 18 4538.0 19 5520.0 20 7026.0 21 9238.0 22 11403.0 23 13405.0 24 15532.0 25 17766.0 26 19912.0 27 22515.0 28 25518.0 29 30117.0 30 36007.0 31 43652.0 32 55833.0 33 73045.0 34 96862.0 35 130765.0 36 186755.0 37 273592.0 38 735163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44259948175614 18.589799962778063 12.283391859031125 25.68420869643467 2 14.476256168524815 20.4265927637652 41.07070853860969 24.026442529100294 3 18.41567439504265 24.954740133144345 29.81665788248297 26.812927589330034 4 11.563060733617032 16.912891882486377 38.86471767342669 32.6593297104699 5 12.68401907745239 38.01905391514003 36.292699001048426 13.00422800635915 6 31.755632203625193 37.106174594228115 18.276531395757264 12.861661806389426 7 27.622683585043024 31.650174755378206 22.706507965843315 18.020633693735455 8 25.303635663750878 36.09279738937145 21.0008158214219 17.602751125455775 9 26.603667144013134 14.884428549913336 20.12788800992472 38.38401629614881 10 13.997752582972419 28.930873802071076 35.49948868965172 21.571884925304783 11 34.28528397444243 22.437771702366092 24.480233214122524 18.796711109068955 12 22.925885817072654 24.85623358806143 31.78285668187683 20.435023912989088 13 28.755278031645215 21.770610490199584 25.69251349184873 23.781597986306476 14 20.683671052040403 21.52027537587459 28.641725449191068 29.154328122893936 15 23.570921569328345 29.83684745364578 23.49968595049763 23.092545026528246 16 22.39473486710808 27.674367194109145 27.525237441013083 22.40566049776969 17 21.77855010179732 27.931836901643926 28.123431358681472 22.166181637877287 18 21.738322366657492 27.387517749471662 29.24010050502704 21.634059378843805 19 23.419434520907163 27.09662629232196 27.440394529321395 22.043544657449477 20 23.640231987831818 27.2180811222174 27.479039351030433 21.662647538920353 21 23.371612099426073 26.79438736953902 26.967586446355263 22.866414084679644 22 23.217373364748944 27.33362542907946 26.972744198682303 22.476257007489295 23 22.49534349866636 27.212246598507466 27.53886544753471 22.75354445529146 24 23.03342009965615 27.141619083075565 27.44430536638727 22.38065545088102 25 22.923189888826933 26.826553267279007 27.46473119729083 22.785525646603226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 339.0 3 443.0 4 443.0 5 443.0 6 875.0 7 1307.0 8 1307.0 9 1307.0 10 2071.5 11 2836.0 12 2836.0 13 2836.0 14 5470.0 15 8104.0 16 8104.0 17 8104.0 18 15141.5 19 22179.0 20 22179.0 21 22179.0 22 35284.0 23 48389.0 24 48389.0 25 48389.0 26 68507.5 27 88626.0 28 88626.0 29 88626.0 30 114345.0 31 140064.0 32 140064.0 33 140064.0 34 161387.0 35 182710.0 36 182710.0 37 182710.0 38 200141.5 39 217573.0 40 217573.0 41 217573.0 42 230182.5 43 242792.0 44 242792.0 45 242792.0 46 252861.5 47 262931.0 48 262931.0 49 262931.0 50 252957.0 51 242983.0 52 242983.0 53 242983.0 54 217252.0 55 191521.0 56 191521.0 57 191521.0 58 164585.5 59 137650.0 60 137650.0 61 137650.0 62 112150.0 63 86650.0 64 86650.0 65 86650.0 66 66890.5 67 47131.0 68 47131.0 69 47131.0 70 34230.5 71 21330.0 72 21330.0 73 21330.0 74 14948.5 75 8567.0 76 8567.0 77 8567.0 78 5952.5 79 3338.0 80 3338.0 81 3338.0 82 2260.0 83 1182.0 84 1182.0 85 1182.0 86 804.0 87 426.0 88 426.0 89 426.0 90 265.0 91 104.0 92 104.0 93 104.0 94 62.0 95 20.0 96 20.0 97 20.0 98 16.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1667599916900745 2 0.24638827055034881 3 0.24669453316134987 4 0.40712509755740256 5 0.24266207544983595 6 0.018733063039564537 7 0.018630975502564186 8 0.017405925058559965 9 0.01827366912306295 10 0.0209279450850721 11 0.01980498217806823 12 0.027767810064095663 13 0.02618545324059021 14 0.02404161496358282 15 0.04149858379064296 16 0.020723770011071393 17 0.04083501480014068 18 0.030319998489104452 19 0.026338584546090737 20 0.028176160212097064 21 0.033842018515616586 22 0.04292780930864788 23 0.02812511644359689 24 0.04951245544517057 25 0.03649629447762573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1959103.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.31136786659961 #Duplication Level Percentage of deduplicated Percentage of total 1 82.77378227668798 59.85485419919407 2 11.59968025223668 16.775774917088352 3 3.033061592130002 6.579744976517006 4 1.1138371644755656 3.2217235577553143 5 0.5109235715675088 1.8472791167667528 6 0.24229581273090348 1.0512444988152654 7 0.15634044352206578 0.7913633926766007 8 0.10614522984435339 0.6140405410047843 9 0.07792335783822854 0.507127013463674 >10 0.35276181895758146 4.36642771499142 >50 0.01945200405786013 0.9484299320928847 >100 0.011367182715576386 1.6349603010767508 >500 0.001643345424210452 0.7997947648567661 >1k 7.859478115788862E-4 1.0072350737004045 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3427 0.17492699465010264 No Hit CCCATGTACTCTGCGTTGATACCAC 3043 0.15532618754603508 No Hit GTATCAACGCAGAGTACTTTTTTTT 1986 0.10137292424134924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.104376850017585E-5 2 0.0 0.0 0.0 0.0 5.104376850017585E-5 3 0.0 0.0 0.0 0.0 5.104376850017585E-5 4 0.0 0.0 0.0 0.0 5.104376850017585E-5 5 0.0 0.0 0.0 0.0 1.020875370003517E-4 6 0.0 0.0 0.0 0.0 1.020875370003517E-4 7 0.0 0.0 0.0 0.0 1.020875370003517E-4 8 0.0 0.0 0.0 0.0 1.020875370003517E-4 9 0.0 0.0 0.0 5.104376850017585E-5 1.020875370003517E-4 10 0.0 0.0 0.0 5.104376850017585E-5 1.020875370003517E-4 11 0.0 0.0 0.0 5.104376850017585E-5 1.020875370003517E-4 12 0.0 0.0 0.0 5.104376850017585E-5 1.020875370003517E-4 13 0.0 0.0 0.0 5.104376850017585E-5 2.552188425008792E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCCGT 60 4.1358412E-4 12.650992 6 ACAGTCG 55 0.0030957505 12.076872 8 TCCAACG 185 0.0 11.799508 18 TAGGACC 385 0.0 10.88595 4 TGTCGCC 70 0.0015049448 10.845645 10 GTCTTAA 185 5.456968E-12 10.836557 1 TCTTATA 190 9.094947E-12 10.53515 2 TGGCGAG 855 0.0 10.323444 18 CGGTTTC 175 2.401066E-10 10.303889 13 GACGTGG 425 0.0 10.270678 7 GTATTAC 160 5.697075E-9 10.1431465 1 CCGGTTT 160 6.2755134E-9 10.083576 12 GTATTAG 190 9.094947E-11 10.048939 1 GTCCTAC 970 0.0 10.038579 1 ATTACAC 250 0.0 9.906467 3 TAGGACT 260 0.0 9.89156 4 GTAGGAC 935 0.0 9.882018 3 CCAACGT 145 1.6399645E-7 9.818182 19 TGCCGGT 175 2.6211637E-9 9.761081 10 GCCGGTT 185 6.6029315E-10 9.7469225 11 >>END_MODULE