FastQCFastQC Report
Thu 26 May 2016
SRR1513154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959103
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38780.19794773424368192No Hit
CCCATGTACTCTGCGTTGATACCAC38290.19544658958717329No Hit
GTATCAACGCAGAGTACTTTTTTTT28980.14792484111350962No Hit
TATCAACGCAGAGTACTTTTTTTTT21710.11081602141388175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3200.012.4947154
CCTATAC1701.0913936E-1111.200033
GGCGAGG5000.010.81681919
TGGACCG803.699802E-410.7097555
ATAGGAC2050.010.68100553
TGGCGAG8450.010.55841918
TCTATAC1409.396172E-810.2000283
ATTACCC856.4681174E-410.0800283
GTTAGAC951.6097414E-410.021083
TATGGCG9250.09.95334116
GTCCTAC10400.09.8904871
TAGTACA1752.4829205E-99.7917774
TTAGGAC7700.09.76733
GTACTAA1401.0225649E-69.5241731
GGTCTAC2600.09.5241721
CCTACAC2106.366463E-119.5200263
ATGTCGA900.00112258439.4918317
GGCGTGC900.00112520869.4894078
TCGAACT1606.76082E-89.48843819
TCCTACA11200.09.4357512
Produced by FastQC (version 0.11.5)