##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513154_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1959103 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.585787475186347 31.0 31.0 34.0 27.0 34.0 2 30.70255213738124 31.0 31.0 34.0 27.0 34.0 3 30.801114591728968 33.0 31.0 34.0 27.0 34.0 4 34.17956432101835 37.0 35.0 37.0 30.0 37.0 5 33.920922993839525 37.0 35.0 37.0 30.0 37.0 6 33.840816945306095 37.0 35.0 37.0 30.0 37.0 7 33.69141132446839 37.0 35.0 37.0 28.0 37.0 8 33.65852382442373 37.0 35.0 37.0 28.0 37.0 9 35.188186634393396 39.0 35.0 39.0 28.0 39.0 10 35.02897142212533 39.0 35.0 39.0 28.0 39.0 11 35.04552644756299 39.0 35.0 39.0 28.0 39.0 12 34.981634962531324 39.0 35.0 39.0 27.0 39.0 13 34.95324237674078 39.0 35.0 39.0 27.0 39.0 14 36.095375791880265 40.0 36.0 41.0 27.0 41.0 15 36.067228726616214 40.0 36.0 41.0 27.0 41.0 16 36.02383131463736 40.0 36.0 41.0 27.0 41.0 17 35.95351392958921 40.0 36.0 41.0 27.0 41.0 18 35.917421901758104 40.0 36.0 41.0 27.0 41.0 19 35.989104707613635 40.0 36.0 41.0 27.0 41.0 20 35.95670875905963 40.0 36.0 41.0 27.0 41.0 21 35.90473548353507 40.0 36.0 41.0 27.0 41.0 22 35.84815652877873 40.0 36.0 41.0 27.0 41.0 23 35.76703573012751 40.0 36.0 41.0 27.0 41.0 24 35.69969368634523 40.0 36.0 41.0 27.0 41.0 25 35.64269974575099 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7536227416560202 1101 2 0.7586786442185272 1101 3 0.7090464585594525 1101 4 0.8013301984254682 1101 5 0.7784929719875464 1101 6 0.7181861549733313 1101 7 0.8960380913209178 1101 8 0.8530710311760927 1101 9 0.9531974174234605 1101 10 1.0797947519601578 1101 11 1.077236122215865 1101 12 1.0044810128350719 1101 13 1.080947730386164 1101 14 1.0879061001116455 1101 15 1.1816924739252102 1101 16 1.1539180250022838 1101 17 1.1713137703011256 1101 18 1.1160899273315366 1101 19 1.1789274081762215 1101 20 1.148148581841454 1101 21 1.1875027823995907 1101 22 1.2001676236158119 1101 23 1.2041850302036536 1101 24 1.2344185221126338 1101 25 1.2354267346609547 1102 1 0.7485356056070742 1102 2 0.7880564459674382 1102 3 0.7317588609679611 1102 4 0.8093541420500827 1102 5 0.8857251314230865 1102 6 0.8021033425700423 1102 7 0.8058383548068875 1102 8 0.8803581866770287 1102 9 0.8860513772285898 1102 10 0.9472741284356374 1102 11 1.026826746359312 1102 12 0.9689974983919711 1102 13 1.0325233411342722 1102 14 1.1654960876446907 1102 15 1.0848631474059687 1102 16 1.1619264954573723 1102 17 1.2509307430418275 1102 18 1.222966203868019 1102 19 1.2866153629768888 1102 20 1.232258520921377 1102 21 1.197506849632724 1102 22 1.170161611184227 1102 23 1.2048127987957713 1102 24 1.3207817967658144 1102 25 1.2731733106695842 1103 1 0.707262001190319 1103 2 0.6329001571891446 1103 3 0.5930757011436931 1103 4 0.8080511913074986 1103 5 0.755847393267203 1103 6 0.6672297625359889 1103 7 0.6772326536082147 1103 8 0.7846501956812091 1103 9 0.8433200354396817 1103 10 0.7825702694373291 1103 11 0.7536980100364872 1103 12 0.7423813822954699 1103 13 0.8691200381487008 1103 14 0.9222365990719013 1103 15 0.9412869013844869 1103 16 0.8362889530597641 1103 17 0.9951740301786387 1103 18 0.8684355926894227 1103 19 0.8106939362403836 1103 20 0.8257531284251556 1103 21 0.7376858448040764 1103 22 0.7645655629238846 1103 23 0.8056973249222708 1103 24 0.8437433374043763 1103 25 0.8737643735377745 1104 1 0.5086063980254316 1104 2 0.530754822417812 1104 3 0.5664079980834167 1104 4 0.7233250421251185 1104 5 0.6914543150810175 1104 6 0.6516161689383111 1104 7 0.5763643500873954 1104 8 0.6919182297396986 1104 9 0.7158510705701886 1104 10 0.8129446990160005 1104 11 0.7703042743881099 1104 12 0.8779368083018255 1104 13 0.8843844966492327 1104 14 0.9320599911234098 1104 15 0.9238544370024186 1104 16 0.872200639369332 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-1.0111644548727625 2216 5 -1.4364602990404904 2216 6 -1.5308197189492745 2216 7 -1.6558592789188395 2216 8 -1.6136732047189497 2216 9 -1.8623733315761655 2216 10 -1.8308682294472547 2216 11 -1.8909436730667366 2216 12 -1.8747647489726091 2216 13 -1.924711603248987 2216 14 -1.9813905190426695 2216 15 -1.9862857567081917 2216 16 -2.030844043164443 2216 17 -2.289698801296616 2216 18 -2.170733108134037 2216 19 -2.343592419076046 2216 20 -2.155976380551408 2216 21 -2.3944548265457684 2216 22 -2.2963412805049686 2216 23 -2.4448625119475835 2216 24 -2.3546462352167907 2216 25 -2.4697993839651744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53448.0 3 0.0 4 875.0 5 7151.0 6 8052.0 7 6197.0 8 4916.0 9 4409.0 10 3721.0 11 3405.0 12 3108.0 13 3093.0 14 2997.0 15 2938.0 16 3017.0 17 3177.0 18 3545.0 19 4118.0 20 5305.0 21 6996.0 22 8888.0 23 10320.0 24 11957.0 25 13532.0 26 14860.0 27 16615.0 28 18631.0 29 22474.0 30 28078.0 31 36114.0 32 45927.0 33 60561.0 34 80025.0 35 106865.0 36 154358.0 37 253985.0 38 945445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.42768425778857 19.462805817864947 13.449747224997877 27.659762699348605 2 13.84344202125433 20.89934260432417 38.98282848598886 26.274386888432634 3 16.837772802495945 24.977922612247568 30.21968149348296 27.964623091773532 4 11.422177711215447 17.444180032729882 37.280877724384986 33.852764531669685 5 12.688952003432705 38.199873316850905 35.99476921190822 13.116405467808177 6 31.95648611836513 37.45578461191844 17.78610710006452 12.80162216965191 7 27.40088461100551 31.75593191262539 22.677513184155426 18.165670292213676 8 25.32692684679975 36.44759572973503 20.596340297971054 17.629137125494168 9 26.36609120886974 14.637267613398535 19.762942695837715 39.23369848189401 10 14.319316767047516 29.18058292867064 34.8862375889403 21.613862715341554 11 34.30498549591318 22.36513343096305 24.348745318648383 18.98113575447539 12 22.663688432920576 24.9924582832041 31.71793417701877 20.625919106856553 13 28.624681805908114 21.652715554006093 25.791650566611352 23.930952073474444 14 21.051086812820525 21.53544756025414 27.558794268933788 29.854671357991542 15 23.505042869108976 29.92216335741408 23.327410554728363 23.24538321874858 16 22.560018539096717 27.861679554367484 27.005930775462033 22.572371131073762 17 21.91338327807554 28.214558854912802 27.54896364830107 22.323094218710594 18 22.05462392756976 27.50930677721278 28.63051111579719 21.805558179420263 19 23.304338771366282 27.10873292522139 27.432605636355007 22.154322667057322 20 23.48896408203142 27.111438244951895 27.545718627351395 21.853879045665288 21 23.19325277539997 26.726122933795182 27.058063271309546 23.022561019495306 22 23.076196684091325 27.425589594410905 26.854409241789984 22.643804479707786 23 22.54189763047594 27.14582585715883 27.242916159818193 23.06936035254704 24 22.9608142093601 27.142115549820506 27.462415197159107 22.434655043660285 25 22.873446286733124 26.9057121724958 27.266898984583204 22.953942556187876 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 182.0 2 298.0 3 414.0 4 414.0 5 414.0 6 907.5 7 1401.0 8 1401.0 9 1401.0 10 2120.5 11 2840.0 12 2840.0 13 2840.0 14 5232.0 15 7624.0 16 7624.0 17 7624.0 18 14413.0 19 21202.0 20 21202.0 21 21202.0 22 34343.5 23 47485.0 24 47485.0 25 47485.0 26 67480.0 27 87475.0 28 87475.0 29 87475.0 30 112918.5 31 138362.0 32 138362.0 33 138362.0 34 159302.5 35 180243.0 36 180243.0 37 180243.0 38 196573.0 39 212903.0 40 212903.0 41 212903.0 42 225799.5 43 238696.0 44 238696.0 45 238696.0 46 251736.5 47 264777.0 48 264777.0 49 264777.0 50 255367.0 51 245957.0 52 245957.0 53 245957.0 54 220338.5 55 194720.0 56 194720.0 57 194720.0 58 167649.5 59 140579.0 60 140579.0 61 140579.0 62 114895.0 63 89211.0 64 89211.0 65 89211.0 66 68933.0 67 48655.0 68 48655.0 69 48655.0 70 35448.0 71 22241.0 72 22241.0 73 22241.0 74 15611.0 75 8981.0 76 8981.0 77 8981.0 78 6180.0 79 3379.0 80 3379.0 81 3379.0 82 2300.5 83 1222.0 84 1222.0 85 1222.0 86 831.5 87 441.0 88 441.0 89 441.0 90 272.0 91 103.0 92 103.0 93 103.0 94 56.5 95 10.0 96 10.0 97 10.0 98 5.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18421696051713463 2 0.008218046728528312 3 0.004338720322514947 4 0.0018886194345065063 5 0.07467703331575726 6 0.31458274526658375 7 0.025623971787088275 8 0.01301616096754484 9 0.004236632785514595 10 0.0032668011840112543 11 0.0 12 0.0 13 0.0 14 0.001327137981004572 15 0.0 16 0.0 17 0.02067272624257122 18 0.029809560804102693 19 0.0 20 0.0 21 8.167002960028136E-4 22 1.020875370003517E-4 23 0.005206464387017936 24 0.0 25 3.062626110010551E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1959103.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.5561505640874 #Duplication Level Percentage of deduplicated Percentage of total 1 80.95615379711948 56.309984226018585 2 12.611869291664766 17.544661586912497 3 3.4319908126269976 7.161482090929444 4 1.2581435952924596 3.5004650138161826 5 0.5949821631536926 2.069233446163233 6 0.32002028411141864 1.3355627439129518 7 0.1930454073629148 0.9399246794168344 8 0.11824898913064233 0.6579955593617676 9 0.0831730113929052 0.5206675052982125 >10 0.3933388711201498 4.777925760453459 >50 0.022331138120255663 1.0613338159948664 >100 0.014055106656858832 1.932371016488569 >500 0.00161793637346005 0.8002945589543645 >1k 0.0010295958740200153 1.3880979962790276 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3878 0.19794773424368192 No Hit CCCATGTACTCTGCGTTGATACCAC 3829 0.19544658958717329 No Hit GTATCAACGCAGAGTACTTTTTTTT 2898 0.14792484111350962 No Hit TATCAACGCAGAGTACTTTTTTTTT 2171 0.11081602141388175 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.020875370003517E-4 0.0 10 0.0 0.0 0.0 1.020875370003517E-4 0.0 11 0.0 0.0 0.0 1.020875370003517E-4 0.0 12 0.0 0.0 0.0 1.5313130550052753E-4 5.104376850017585E-5 13 0.0 0.0 0.0 1.5313130550052753E-4 5.104376850017585E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 320 0.0 12.494715 4 CCTATAC 170 1.0913936E-11 11.20003 3 GGCGAGG 500 0.0 10.816819 19 TGGACCG 80 3.699802E-4 10.709755 5 ATAGGAC 205 0.0 10.6810055 3 TGGCGAG 845 0.0 10.558419 18 TCTATAC 140 9.396172E-8 10.200028 3 ATTACCC 85 6.4681174E-4 10.080028 3 GTTAGAC 95 1.6097414E-4 10.02108 3 TATGGCG 925 0.0 9.953341 16 GTCCTAC 1040 0.0 9.890487 1 TAGTACA 175 2.4829205E-9 9.791777 4 TTAGGAC 770 0.0 9.7673 3 GTACTAA 140 1.0225649E-6 9.524173 1 GGTCTAC 260 0.0 9.524172 1 CCTACAC 210 6.366463E-11 9.520026 3 ATGTCGA 90 0.0011225843 9.49183 17 GGCGTGC 90 0.0011252086 9.489407 8 TCGAACT 160 6.76082E-8 9.488438 19 TCCTACA 1120 0.0 9.435751 2 >>END_MODULE