FastQCFastQC Report
Thu 26 May 2016
SRR1513153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161669
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24840.11491120981056767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5150.011.8409714
GTCTACG803.5978522E-410.7422841
CCGTTCA803.8099953E-410.67599
TGGACCG1252.2083623E-710.6513795
CGCAGAT1252.2828863E-710.6257478
CGTGCGC1201.5456426E-610.28049610
GTAGGAC9250.010.1978373
ACTGTCG750.002677898210.1197598
GTCCTAG2750.010.0695351
GCCTACG1053.881839E-510.0033971
TAGACTG3350.09.9359875
TGTAGGA10950.09.9263132
GGACCGT1151.04465435E-59.8986196
GTGCGCC1951.6734703E-109.73323911
GTCCTAC8800.09.6572051
GTCCTAA5150.09.6414021
TGGCGAG7800.09.61179518
AGGACGT6750.09.5806055
AGGACCT7550.09.573145
GGACGTG6650.09.5575016
Produced by FastQC (version 0.11.5)