##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513153_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2161669
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.32504513873308	31.0	30.0	34.0	24.0	34.0
2	29.192068720974394	31.0	30.0	34.0	22.0	34.0
3	29.364962905976817	31.0	30.0	34.0	23.0	34.0
4	32.43133754520234	35.0	33.0	37.0	20.0	37.0
5	32.585610933033685	37.0	33.0	37.0	24.0	37.0
6	32.92525960264962	37.0	35.0	37.0	26.0	37.0
7	32.89094028734279	37.0	35.0	37.0	26.0	37.0
8	32.76576987503637	37.0	35.0	37.0	25.0	37.0
9	33.9946023188564	39.0	34.0	39.0	23.0	39.0
10	33.57352813959954	38.0	34.0	39.0	18.0	39.0
11	34.03073412256918	39.0	34.0	39.0	25.0	39.0
12	34.01922172173445	39.0	35.0	39.0	25.0	39.0
13	34.066927915420905	39.0	35.0	39.0	25.0	39.0
14	34.27868512709393	39.0	33.0	41.0	17.0	41.0
15	34.70912891844218	39.0	34.0	41.0	19.0	41.0
16	34.550021765589456	39.0	34.0	41.0	17.0	41.0
17	34.14239367821808	39.0	33.0	41.0	17.0	41.0
18	33.86660399903963	39.0	33.0	41.0	15.0	41.0
19	34.50811664505528	39.0	34.0	41.0	18.0	41.0
20	34.71895928562606	39.0	34.0	41.0	19.0	41.0
21	34.766351370168145	40.0	35.0	41.0	19.0	41.0
22	34.44664099822868	40.0	34.0	41.0	17.0	41.0
23	33.983084366755506	39.0	34.0	41.0	10.0	41.0
24	34.22064155057967	39.0	34.0	41.0	10.0	41.0
25	33.8657597439756	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.861539370860541
1101	2	1.0789676723580826
1101	3	1.0652097148269846
1101	4	1.366498728386567
1101	5	1.2441918738272761
1101	6	0.932352395745724
1101	7	0.9469871507995009
1101	8	1.0566514444573443
1101	9	1.2861573903261885
1101	10	1.696089157256182
1101	11	1.326819385401521
1101	12	1.176632922983849
1101	13	0.9890862440196955
1101	14	2.091770200730842
1101	15	1.7107385514384745
1101	16	1.7575284815444547
1101	17	2.2164650467378877
1101	18	2.213862179289869
1101	19	1.8462293584213327
1101	20	1.4437059688099154
1101	21	1.4422870948755104
1101	22	1.6818156837470113
1101	23	1.958079211534276
1101	24	1.7052785642406576
1101	25	1.9609929897195784
1102	1	1.0719778236857707
1102	2	1.3454152775236032
1102	3	1.1922503219374754
1102	4	1.5940199416623813
1102	5	1.485798495490343
1102	6	1.1685832973979373
1102	7	1.1484498908995207
1102	8	1.255614618988318
1102	9	1.5052433082160306
1102	10	1.813925636952625
1102	11	1.4370232701249606
1102	12	1.3240351243304147
1102	13	1.350214592891625
1102	14	2.322341931557915
1102	15	1.8728924034995984
1102	16	2.016123642598963
1102	17	2.38369758125976
1102	18	2.543867156170265
1102	19	2.037004260175941
1102	20	1.8906211644416828
1102	21	1.5373040610657114
1102	22	1.7857199504250971
1102	23	2.1408971103849623
1102	24	1.932661153429457
1102	25	2.224557548620787
1103	1	0.9021317113193703
1103	2	1.2241028220326164
1103	3	0.9600159766559422
1103	4	1.457630249291114
1103	5	1.271301524301414
1103	6	1.0140119466976643
1103	7	0.9366769447616576
1103	8	1.0301981025166853
1103	9	1.4044303501211814
1103	10	1.555708576555169
1103	11	1.2203555777361075
1103	12	1.202539504164129
1103	13	1.211434967837043
1103	14	2.0308146898420887
1103	15	1.6815108143335635
1103	16	1.6225206322508967
1103	17	1.908179518292144
1103	18	2.1699873180585314
1103	19	1.6449465735604463
1103	20	1.3621104837431375
1103	21	1.385840648575929
1103	22	1.573697900328824
1103	23	1.9621412820849926
1103	24	1.5376054274086428
1103	25	1.830828227912626
1104	1	0.8597125110843713
1104	2	0.9984275903203041
1104	3	0.8483337336652887
1104	4	1.283220255707171
1104	5	1.1653191336642195
1104	6	0.9523022615481196
1104	7	0.987474313406473
1104	8	0.9883549943636609
1104	9	1.320891780360121
1104	10	1.492649394254201
1104	11	1.156779632527666
1104	12	1.0888195842488528
1104	13	1.0264539454426966
1104	14	1.9910751584459376
1104	15	1.6569417721808648
1104	16	1.660113684360077
1104	17	2.0913004647760687
1104	18	2.115230741603135
1104	19	1.5962964572211504
1104	20	1.3702948177489915
1104	21	1.256287955765778
1104	22	1.5582640050110044
1104	23	1.8935820727283783
1104	24	1.5629708719329685
1104	25	1.8533462585705465
1105	1	0.6481469693655306
1105	2	0.9218131506303635
1105	3	0.8639684085878905
1105	4	1.0662999444195407
1105	5	0.9901483940872069
1105	6	0.7626399763650156
1105	7	0.676778986562141
1105	8	0.8462962092458497
1105	9	1.1124673475074403
1105	10	1.4964857531414992
1105	11	0.9319129207913832
1105	12	0.9003134883273987
1105	13	0.9020679549226784
1105	14	1.5533178967323238
1105	15	1.4069373548464768
1105	16	1.6042543783809222
1105	17	1.894460779443925
1105	18	1.4148191612729448
1105	19	1.415823187855942
1105	20	1.2790438286502095
1105	21	1.1666996129191674
1105	22	1.378315822263957
1105	23	1.5345716708661854
1105	24	1.410351200582646
1105	25	1.5695713198370527
1106	1	0.6495934710443478
1106	2	0.9616858166173436
1106	3	0.784871614739842
1106	4	1.2663130273212104
1106	5	1.1791367960948733
1106	6	0.7331042978904065
1106	7	0.7266037224043416
1106	8	0.8160218140730677
1106	9	0.9889282170157756
1106	10	0.963513570193939
1106	11	0.8432956993941119
1106	12	0.9342845508178783
1106	13	0.8630451168488165
1106	14	1.879691718219867
1106	15	1.3957600712493488
1106	16	1.527561380817147
1106	17	1.9601250777938901
1106	18	1.9456303292116104
1106	19	1.5604452274990024
1106	20	1.3002715842130073
1106	21	1.1006907670086505
1106	22	1.3640440586233993
1106	23	1.8111431843429742
1106	24	1.3515020660633112
1106	25	1.6507714704773164
1107	1	0.8023646545429663
1107	2	0.9621286983762509
1107	3	0.9250604603920145
1107	4	1.2600382647832475
1107	5	1.1446505742859827
1107	6	0.863985284521469
1107	7	0.7559494883332647
1107	8	0.9052675645020329
1107	9	1.0584114340088036
1107	10	1.3705018745260418
1107	11	0.9278574721538959
1107	12	0.8700649735587618
1107	13	0.8413050248099054
1107	14	1.7473648848969603
1107	15	1.136996206267554
1107	16	1.3168149265451916
1107	17	1.6298404315418438
1107	18	1.9095792464355057
1107	19	1.388366629130033
1107	20	1.1651640045536666
1107	21	1.0942630897086545
1107	22	1.2894622628221626
1107	23	1.60949892009684
1107	24	1.3985102574723527
1107	25	1.5605102463796925
1108	1	0.6021021845144503
1108	2	0.750721610424474
1108	3	0.6568220360048223
1108	4	1.1910493598615233
1108	5	1.0888262436566123
1108	6	0.6833055646095971
1108	7	0.6165460176760433
1108	8	0.6533476782090233
1108	9	0.8408277435396911
1108	10	1.0984811435371
1108	11	0.8618814781635038
1108	12	0.5369191702869784
1108	13	0.6368147516933576
1108	14	1.569401738787441
1108	15	1.2097027460406835
1108	16	1.2200611241525152
1108	17	1.5163584432548163
1108	18	1.8623695892678427
1108	19	1.2960488764643543
1108	20	1.0116927168292307
1108	21	1.0056011824504623
1108	22	1.3395282856536284
1108	23	1.5340341932223964
1108	24	1.2435462953098266
1108	25	1.5698411729871609
1109	1	0.5338638226601766
1109	2	0.6769169012859209
1109	3	0.6182801462880931
1109	4	0.8748595463208133
1109	5	0.9375628077459979
1109	6	0.6039228106155861
1109	7	0.6017098363561928
1109	8	0.6656035181991058
1109	9	0.858032102608334
1109	10	1.239987108494752
1109	11	0.7627075052772199
1109	12	0.6489027872581374
1109	13	0.6226873896099505
1109	14	1.6248920697179798
1109	15	1.0917226504849182
1109	16	1.2185908912565537
1109	17	1.5395516908809697
1109	18	1.7941793571148068
1109	19	1.1709035274454394
1109	20	1.069411926187712
1109	21	0.9269242116820706
1109	22	1.17921244766017
1109	23	1.558339156582207
1109	24	1.339798911413581
1109	25	1.4254380500392614
1110	1	0.4159593062017528
1110	2	0.6085370425874466
1110	3	0.45730047950851826
1110	4	0.8418121407970247
1110	5	0.6824700445876388
1110	6	0.47646486624095274
1110	7	0.39747973592051977
1110	8	0.37808271932431836
1110	9	0.52247668691016
1110	10	0.8092748517788237
1110	11	0.5755244384075553
1110	12	0.3904845023512067
1110	13	0.3547193892931517
1110	14	1.171614669122988
1110	15	0.8266738292587164
1110	16	1.0757272830822444
1110	17	1.3496103201953389
1110	18	0.9240473368537181
1110	19	0.7128974544922144
1110	20	0.4873444985868929
1110	21	0.39200994582842696
1110	22	0.6584471622108623
1110	23	-0.573308174408595
1110	24	0.11906442208827173
1110	25	0.5810085950846897
1111	1	0.24171978992382037
1111	2	0.6742507561441293
1111	3	0.640414388253248
1111	4	0.9870379120049861
1111	5	0.7774029643148168
1111	6	0.5589349390269405
1111	7	0.4307779696491778
1111	8	0.4655478915854019
1111	9	0.7528145454779605
1111	10	0.8669677735050882
1111	11	0.4646280241202376
1111	12	0.32497833227106554
1111	13	0.2122217042011556
1111	14	1.2226512759051502
1111	15	0.8518655466775158
1111	16	0.9166760968914573
1111	17	1.280329670049781
1111	18	1.4358413940302057
1111	19	0.9651901504889153
1111	20	0.5200777142891155
1111	21	0.3547036588575807
1111	22	0.6793403349328457
1111	23	1.0736175097560476
1111	24	0.7281576722746479
1111	25	0.9089947917081886
1112	1	0.15573283972933538
1112	2	0.3151237240985516
1112	3	0.24266667345277426
1112	4	0.6199465792370162
1112	5	0.31666485539116707
1112	6	0.1694458218001884
1112	7	0.09267263414731985
1112	8	0.1316361999615765
1112	9	0.3812720159602918
1112	10	0.6344339293671837
1112	11	0.22376140442145953
1112	12	0.10832720273008078
1112	13	0.0294179901955971
1112	14	0.4366292377976748
1112	15	0.32585460421686463
1112	16	0.36939194275856835
1112	17	0.7365229109515923
1112	18	0.807378403325572
1112	19	0.40757645288531563
1112	20	0.19312667797088068
1112	21	0.23293411019785282
1112	22	0.4129397544814708
1112	23	0.7973114079093264
1112	24	0.5362248906415275
1112	25	0.7150512691025313
1113	1	-0.12434177807666202
1113	2	0.07561422001790419
1113	3	-0.09697649062833236
1113	4	0.07492845354468614
1113	5	0.1346602752190691
1113	6	-0.09121258739647686
1113	7	-0.1675453143634016
1113	8	-0.1231909843468415
1113	9	0.039209972133299686
1113	10	0.29597062903644655
1113	11	-0.0718722313334581
1113	12	-0.1970727438265527
1113	13	-0.33000059482079536
1113	14	0.5039990022651253
1113	15	0.08234802473327107
1113	16	0.1775453919362988
1113	17	0.46024082902642505
1113	18	0.3661849345588166
1113	19	0.23237326320366236
1113	20	0.03595142465655243
1113	21	-0.07983218237671963
1113	22	-0.026385947753695405
1113	23	0.18044720598832242
1113	24	0.09462629777714682
1113	25	0.5265209410242377
1114	1	-1.0465777755861474
1114	2	-0.665360730270919
1114	3	-0.6468182200250148
1114	4	-0.4647176054140161
1114	5	-3.548966631921676
1114	6	-1.2502891925605368
1114	7	-0.8786667487431785
1114	8	-0.6324033915480811
1114	9	-0.48377402206956077
1114	10	-0.34828536423225387
1114	11	-0.7896168762900615
1114	12	-0.8152833269849111
1114	13	-0.7569092531438812
1114	14	-6.019432592754356
1114	15	-2.44430260339594
1114	16	-1.4194475298330431
1114	17	-0.5373533903553067
1114	18	-6.324908787184221
1114	19	-2.511012779221879
1114	20	-1.5794929534173292
1114	21	-1.2841630933485035
1114	22	-1.081086578348966
1114	23	-0.5821743740366543
1114	24	-0.6685831745993767
1114	25	-0.5981247996649017
1115	1	-1.0993524090552853
1115	2	-0.7041499565162646
1115	3	-0.6907201717252498
1115	4	-3.4902887949497554
1115	5	-1.4646869810911802
1115	6	-1.0073137372726038
1115	7	-0.8873383539644806
1115	8	-0.7096716321203331
1115	9	-0.48351316385638654
1115	10	-0.7889204190546693
1115	11	-0.9214943549207035
1115	12	-0.9502513824011629
1115	13	-0.9687743615071085
1115	14	-6.495258431488736
1115	15	-2.8041821007523353
1115	16	-1.5230239222045512
1115	17	-0.685944660480871
1115	18	-0.48037407322077996
1115	19	-0.877237093404382
1115	20	-0.8139353918724623
1115	21	-0.8747435406841149
1115	22	-2.159825638940987
1115	23	-1.2510219968597198
1115	24	-1.0541062203916525
1115	25	-0.7050667167220226
1116	1	-0.28427118523919503
1116	2	-0.04830736315912887
1116	3	-0.1504658619879855
1116	4	-0.03176415173486191
1116	5	-0.5416765413057334
1116	6	-0.31260927819894846
1116	7	-0.2747255384751952
1116	8	-0.15960891167087965
1116	9	-0.18886320985416205
1116	10	0.14457109158236392
1116	11	-0.222597759423401
1116	12	-0.5717459806858542
1116	13	-0.47485290719336604
1116	14	0.42677075020122857
1116	15	-0.1280366810549438
1116	16	-0.11829311553783839
1116	17	0.35969947936904845
1116	18	0.15231887765125407
1116	19	-0.11647870141658245
1116	20	-0.4359948495240644
1116	21	-0.3801143961927167
1116	22	-0.4868256247621616
1116	23	-1.1101007402154792
1116	24	-0.8088094200520288
1116	25	-0.15931936336652086
1201	1	0.9317267268956932
1201	2	1.1580592083254544
1201	3	1.1565891853786852
1201	4	1.5146395950855123
1201	5	1.417718969784282
1201	6	1.1115197348596553
1201	7	1.0005398731105615
1201	8	1.1077681959746286
1201	9	1.3181375152302124
1201	10	1.7962070189227717
1201	11	1.2054023067080735
1201	12	1.2037856100831306
1201	13	1.083086058179724
1201	14	2.027699425044581
1201	15	1.6612615931497672
1201	16	1.6611499157399905
1201	17	2.0306094248341964
1201	18	1.434784825846691
1201	19	1.4206475081398864
1201	20	1.4502724775126055
1201	21	1.2565571594793568
1201	22	1.5360031962789833
1201	23	1.6883095063840585
1201	24	1.3619044928292041
1201	25	1.7302051749531273
1202	1	0.8988322041821597
1202	2	0.9614104428072494
1202	3	0.940062589386681
1202	4	1.3134452165110915
1202	5	1.1405685071442093
1202	6	0.8507720713082563
1202	7	0.7578084368340043
1202	8	0.8504403492270569
1202	9	1.103835776261505
1202	10	1.5131131425104414
1202	11	0.9981227000683717
1202	12	0.9458364539944171
1202	13	0.9798190985851676
1202	14	1.8186895677888018
1202	15	1.4428100424918426
1202	16	1.5257544630966677
1202	17	1.8523046868419186
1202	18	2.003116066837997
1202	19	1.4704376927309468
1202	20	1.3547436961855936
1202	21	1.274476325332337
1202	22	1.5182485117576618
1202	23	1.7653082742270882
1202	24	1.4232368497362557
1202	25	1.756058966741847
1203	1	0.716903506878797
1203	2	0.8990293138945766
1203	3	0.7967292905921646
1203	4	1.1258051424291722
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2211	12	-0.7251181452168538
2211	13	-0.62233851148779
2211	14	-0.15022868722744676
2211	15	-0.4692819217263917
2211	16	-7.024009007308237
2211	17	-2.236589537300997
2211	18	-0.9673733093374253
2211	19	-0.9811790559652493
2211	20	-1.118361343851042
2211	21	-0.9779120344771997
2211	22	-0.877886319003629
2211	23	-0.5166690709505062
2211	24	-0.8853403491664622
2211	25	-7.589255068533074
2212	1	-1.6454401188715622
2212	2	-4.232322547294505
2212	3	-1.8552086589014927
2212	4	-3.8200304799196445
2212	5	-1.3968337671983626
2212	6	-1.1756506929193868
2212	7	-1.1125111325663823
2212	8	-0.9593304830228426
2212	9	-1.1384772665384304
2212	10	-0.5483825150900614
2212	11	-1.0791033213922674
2212	12	-1.0662535749549136
2212	13	-1.283103006915205
2212	14	-6.588111357896359
2212	15	-5.2830088161764905
2212	16	-2.9566895884227407
2212	17	-7.453622125091446
2212	18	-3.1562785974711183
2212	19	-2.061093458134934
2212	20	-2.131203094252456
2212	21	-1.7139731953568997
2212	22	-1.6825982994823008
2212	23	-1.1954031945613721
2212	24	-1.520049765965723
2212	25	-1.2620462822743974
2213	1	-0.3391468740213739
2213	2	-0.3229079566774402
2213	3	-0.38008761783953915
2213	4	0.029932520197093027
2213	5	-0.12783076565703055
2213	6	-0.35195365271219003
2213	7	-0.4087342754987091
2213	8	-0.38157038076862193
2213	9	-0.20971836529646026
2213	10	-4.3009977341115615
2213	11	-1.7195787204271014
2213	12	-1.014940474819774
2213	13	-0.9156476689400677
2213	14	-0.13645061714682782
2213	15	-0.6201381001001423
2213	16	-0.35147141772070967
2213	17	0.04189337034094365
2213	18	0.1764571976405449
2213	19	-0.40487431198143753
2213	20	-0.7662150746959426
2213	21	-0.7634150313425536
2213	22	-0.9538183261143018
2213	23	-1.2305837520130893
2213	24	-0.6699715122019256
2213	25	-0.4209642434423273
2214	1	-1.0532297814603275
2214	2	-0.8300061963140095
2214	3	-0.80825343271745
2214	4	-0.3851725337495324
2214	5	-3.4966552971732128
2214	6	-1.4922148679308158
2214	7	-1.3011249626083092
2214	8	-1.0590263716828723
2214	9	-0.8800761165741449
2214	10	-4.605086320142224
2214	11	-2.0750585614265376
2214	12	-1.538222700827724
2214	13	-1.2132058745050855
2214	14	-0.4308855972717538
2214	15	-0.705316975738981
2214	16	-0.6731636380500703
2214	17	-0.4847960106925555
2214	18	-0.13018675465928453
2214	19	-1.0005585822824017
2214	20	-1.3587761195568149
2214	21	-1.326731328900756
2214	22	-1.0020863556429802
2214	23	-0.790776824131207
2214	24	-1.0924587353705775
2214	25	-0.8309585648506186
2215	1	-2.2691430745388246
2215	2	-4.866696437547823
2215	3	-3.0319066240764663
2215	4	-2.023236905300198
2215	5	-1.9552196704696776
2215	6	-2.1320861362564933
2215	7	-2.258810884112968
2215	8	-2.558917973747228
2215	9	-8.07805851833594
2215	10	-4.086418545055086
2215	11	-3.3498858944659418
2215	12	-3.645838520768436
2215	13	-3.033143095753367
2215	14	-8.087013540482026
2215	15	-4.938737884446969
2215	16	-9.701850524820095
2215	17	-4.858039622911718
2215	18	-3.2764503270172085
2215	19	-3.7242152652293896
2215	20	-3.400163816280024
2215	21	-3.634391333300158
2215	22	-9.55065862945077
2215	23	-10.641524769887162
2215	24	-6.780441864017501
2215	25	-5.220435204381296
2216	1	-2.1897163505127217
2216	2	-2.060816240233244
2216	3	-2.0976050849001133
2216	4	-7.015948164684197
2216	5	-3.8287581192176496
2216	6	-2.961464215611578
2216	7	-2.562370730619868
2216	8	-8.140896479288593
2216	9	-4.376708496047836
2216	10	-2.579241539774337
2216	11	-2.6187769787527095
2216	12	-2.7515823066328267
2216	13	-2.520113989140757
2216	14	-1.857333009199671
2216	15	-2.51632230211424
2216	16	-2.2248949795634516
2216	17	-1.9813946959907511
2216	18	-7.924110931083785
2216	19	-5.30505194767462
2216	20	-4.748671191401783
2216	21	-3.751639141636616
2216	22	-3.377659605881984
2216	23	-2.581753883339509
2216	24	-3.008414230306567
2216	25	-2.416381835575681
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103441.0
3	2.0
4	869.0
5	5027.0
6	6045.0
7	5460.0
8	5103.0
9	4677.0
10	4456.0
11	4303.0
12	4147.0
13	4241.0
14	4244.0
15	4301.0
16	4386.0
17	4624.0
18	5379.0
19	6491.0
20	8310.0
21	10731.0
22	13144.0
23	15754.0
24	17961.0
25	20721.0
26	23604.0
27	26225.0
28	29560.0
29	34969.0
30	42249.0
31	50647.0
32	64466.0
33	83395.0
34	109625.0
35	145609.0
36	205792.0
37	297031.0
38	784680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93464684094114	18.076524013444324	12.001045442939251	25.987783702675287
2	14.815462395642651	20.046999939244206	40.62030273081056	24.517234934302582
3	18.46635658109319	24.52072483585069	29.560141078422458	27.45277750463366
4	11.577867138132394	16.489727587251835	38.74505713919207	33.1873481354237
5	12.949794113472743	37.66039808129897	36.2088029825022	13.18100482272609
6	32.30990823922209	36.816606146750615	18.04608582353355	12.827399790493747
7	28.30235384859543	31.33316583914358	22.355893690234765	18.008586622026225
8	25.562175570064372	35.756684315069094	20.736957679461028	17.944182435405505
9	26.653208511488863	14.764210067520581	19.769237211320952	38.8133442096696
10	13.720338720327153	28.64075536058094	35.68431879984805	21.95458711924386
11	34.87116892563115	22.11886596635914	24.11137860146092	18.898586506548785
12	22.95604629005367	24.65701002605655	31.61268797988403	20.774255704005746
13	28.90538672115764	21.361746746552207	25.56929552281902	24.163571009471138
14	20.740575189565096	20.99905095989091	28.647361034569396	29.6130128159746
15	23.71780305747447	29.356540138087556	23.341507763549014	23.584149040888967
16	22.870561235231108	27.57157432277677	26.8259752037627	22.73188923822942
17	22.151013566359648	27.575613688722527	27.813212414371435	22.460160330546383
18	22.119527568601626	27.18257503154803	28.669207784761713	22.028689615088634
19	23.500044884591073	26.97346441484838	27.276643632079615	22.24984706848093
20	23.86524912946403	26.762346571650024	27.24679260998832	22.125611688897628
21	23.722215640687704	26.477328258970218	26.728192702394526	23.072263397947548
22	23.52678738782184	26.91216006390412	26.792247274888155	22.768805273385887
23	22.82192026772394	26.746295288547323	27.253645480492654	23.178138963236083
24	23.154246710132	26.83041067753254	27.253154029182898	22.76218858315256
25	23.268193627267234	26.517358093455762	27.10285650291037	23.111591776366634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	186.0
2	291.0
3	396.0
4	396.0
5	396.0
6	783.5
7	1171.0
8	1171.0
9	1171.0
10	1830.0
11	2489.0
12	2489.0
13	2489.0
14	4970.0
15	7451.0
16	7451.0
17	7451.0
18	14354.0
19	21257.0
20	21257.0
21	21257.0
22	34594.5
23	47932.0
24	47932.0
25	47932.0
26	69154.0
27	90376.0
28	90376.0
29	90376.0
30	116284.5
31	142193.0
32	142193.0
33	142193.0
34	167517.0
35	192841.0
36	192841.0
37	192841.0
38	214990.0
39	237139.0
40	237139.0
41	237139.0
42	253009.5
43	268880.0
44	268880.0
45	268880.0
46	280007.0
47	291134.0
48	291134.0
49	291134.0
50	283578.5
51	276023.0
52	276023.0
53	276023.0
54	249054.0
55	222085.0
56	222085.0
57	222085.0
58	191566.5
59	161048.0
60	161048.0
61	161048.0
62	131262.0
63	101476.0
64	101476.0
65	101476.0
66	78376.0
67	55276.0
68	55276.0
69	55276.0
70	40448.0
71	25620.0
72	25620.0
73	25620.0
74	18035.0
75	10450.0
76	10450.0
77	10450.0
78	7274.0
79	4098.0
80	4098.0
81	4098.0
82	2779.0
83	1460.0
84	1460.0
85	1460.0
86	979.5
87	499.0
88	499.0
89	499.0
90	327.0
91	155.0
92	155.0
93	155.0
94	89.5
95	24.0
96	24.0
97	24.0
98	17.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17264437802457266
2	0.254247990788599
3	0.2639164460423867
4	0.4663526192030325
5	0.2603081230290114
6	0.018365438927051274
7	0.018504220581411865
8	0.01748648844943421
9	0.01859674168431892
10	0.022020022491880117
11	0.019799516022110695
12	0.027756330872117794
13	0.02669233818868661
14	0.024888176681998955
15	0.040061637558756684
16	0.021002290359902465
17	0.04084806693346669
18	0.0313646538854931
19	0.026414774879965432
20	0.0297917951360731
21	0.03427906862706548
22	0.043114833954689645
23	0.02946797127589839
24	0.04718576248260025
25	0.03585192737648548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2161669.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.59547556882342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.6086464442918	64.8063951163497
2	10.766626596452424	16.49349768854432
3	2.588085416325051	5.9470689982846086
4	0.8723051563838127	2.6725851317742007
5	0.3880205712867871	1.4860310094098999
6	0.22688089577410595	1.042683006557898
7	0.12790641089510002	0.6857936659568066
8	0.08368693174026984	0.5128032268433306
9	0.0656028885998499	0.4522396005894643
>10	0.252524975163459	3.2527437509024315
>50	0.011725629231411542	0.6168663348137813
>100	0.006647948478625686	1.0345774133674874
>500	9.746439107606549E-4	0.5410498921638588
>1k	3.654914665351399E-4	0.45566516444214294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2484	0.11491120981056767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.626055145352966E-5
2	0.0	0.0	0.0	0.0	4.626055145352966E-5
3	0.0	0.0	0.0	0.0	4.626055145352966E-5
4	0.0	0.0	0.0	4.626055145352966E-5	4.626055145352966E-5
5	0.0	0.0	0.0	4.626055145352966E-5	4.626055145352966E-5
6	0.0	0.0	0.0	4.626055145352966E-5	4.626055145352966E-5
7	0.0	0.0	0.0	4.626055145352966E-5	4.626055145352966E-5
8	0.0	0.0	0.0	4.626055145352966E-5	4.626055145352966E-5
9	0.0	0.0	0.0	9.252110290705932E-5	9.252110290705932E-5
10	0.0	0.0	0.0	1.3878165436058897E-4	9.252110290705932E-5
11	0.0	0.0	0.0	1.3878165436058897E-4	9.252110290705932E-5
12	0.0	0.0	0.0	1.3878165436058897E-4	2.7756330872117795E-4
13	0.0	0.0	0.0	1.8504220581411863E-4	2.7756330872117795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	515	0.0	11.840971	4
GTCTACG	80	3.5978522E-4	10.742284	1
CCGTTCA	80	3.8099953E-4	10.6759	9
TGGACCG	125	2.2083623E-7	10.651379	5
CGCAGAT	125	2.2828863E-7	10.625747	8
CGTGCGC	120	1.5456426E-6	10.280496	10
GTAGGAC	925	0.0	10.197837	3
ACTGTCG	75	0.0026778982	10.119759	8
GTCCTAG	275	0.0	10.069535	1
GCCTACG	105	3.881839E-5	10.003397	1
TAGACTG	335	0.0	9.935987	5
TGTAGGA	1095	0.0	9.926313	2
GGACCGT	115	1.04465435E-5	9.898619	6
GTGCGCC	195	1.6734703E-10	9.733239	11
GTCCTAC	880	0.0	9.657205	1
GTCCTAA	515	0.0	9.641402	1
TGGCGAG	780	0.0	9.611795	18
AGGACGT	675	0.0	9.580605	5
AGGACCT	755	0.0	9.57314	5
GGACGTG	665	0.0	9.557501	6
>>END_MODULE