FastQCFastQC Report
Thu 26 May 2016
SRR1513153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161669
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC26260.12148020811696889No Hit
GTATCAACGCAGAGTACTTTTTTTT25350.11727049793469768No Hit
CCCATGTACTCTGCGTTGATACCAC25220.1166691107658018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC1251.8559149E-811.39039213
GTCCTAC10150.010.5091851
TACACCG1002.3475892E-510.4707535
ACTATAC2650.010.4168723
GTATTAG2200.010.3897631
AGGACCG1105.9060785E-610.3842175
TGGCGAG8000.010.32206518
CCTAGAC2500.010.28037553
CCTATAC2151.8189894E-1210.1829743
TATGGCG8900.010.0250216
GGCGAGA5250.09.94100319
GTCCTAA5850.09.9309561
CGTTGAT6150.09.87784714
TCGAACT1651.0417352E-89.7766919
GCGTTGA6150.09.72350513
ACTGTGC4700.09.69148358
TCCTACA12200.09.6760352
GAACTGT3350.09.6598186
ATGGCGA9000.09.59768217
CTGCGTT6650.09.56247611
Produced by FastQC (version 0.11.5)