##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513153_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2161669 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.811154251645373 31.0 31.0 34.0 27.0 34.0 2 30.895415995695917 33.0 31.0 34.0 27.0 34.0 3 31.004441012939537 33.0 31.0 34.0 27.0 34.0 4 34.40902145518116 37.0 35.0 37.0 31.0 37.0 5 34.164204140411876 37.0 35.0 37.0 30.0 37.0 6 34.08037308209536 37.0 35.0 37.0 30.0 37.0 7 33.939350566622366 37.0 35.0 37.0 30.0 37.0 8 33.90632377112315 37.0 35.0 37.0 29.0 37.0 9 35.467880605217545 39.0 35.0 39.0 30.0 39.0 10 35.31455602129651 39.0 35.0 39.0 29.0 39.0 11 35.3380563814349 39.0 35.0 39.0 29.0 39.0 12 35.275324760636344 39.0 35.0 39.0 29.0 39.0 13 35.246942524503055 39.0 35.0 39.0 29.0 39.0 14 36.411667096118784 40.0 37.0 41.0 29.0 41.0 15 36.39213912953371 40.0 37.0 41.0 29.0 41.0 16 36.343283361143634 40.0 36.0 41.0 28.0 41.0 17 36.271819598652705 40.0 36.0 41.0 28.0 41.0 18 36.222990661382475 40.0 36.0 41.0 27.0 41.0 19 36.30303806919561 40.0 37.0 41.0 28.0 41.0 20 36.268271876961734 40.0 37.0 41.0 28.0 41.0 21 36.21597663657109 40.0 37.0 41.0 28.0 41.0 22 36.16240738059342 40.0 37.0 41.0 27.0 41.0 23 36.08243630269019 40.0 37.0 41.0 27.0 41.0 24 36.016346165856106 40.0 37.0 41.0 27.0 41.0 25 35.961876679547146 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4535732933540224 1101 2 0.47934732513422773 1101 3 0.4255888287897136 1101 4 0.5473947493648126 1101 5 0.37982087490099303 1101 6 0.27985291905930865 1101 7 0.4387609614899617 1101 8 0.47936441304746324 1101 9 0.5052213853934475 1101 10 0.5208776075705046 1101 11 0.5896883817552663 1101 12 0.5290601930070125 1101 13 0.5839635534463596 1101 14 0.5843641602137168 1101 15 0.6941319704114903 1101 16 0.5809168451595497 1101 17 0.45231357600942346 1101 18 0.46883405986239524 1101 19 0.5133009245016282 1101 20 0.5283410594504474 1101 21 0.6349540794075565 1101 22 0.6145613160342549 1101 23 0.6649134845520877 1101 24 0.5924398329001406 1101 25 0.6105448406168463 1102 1 0.48896910302748253 1102 2 0.6099298367407968 1102 3 0.5049907958143116 1102 4 0.6299000056101534 1102 5 0.5475041346148899 1102 6 0.6214934583345766 1102 7 0.5583650952051045 1102 8 0.6670039293793621 1102 9 0.579237983855684 1102 10 0.7007487600259878 1102 11 0.7198897755771441 1102 12 0.6942333525088884 1102 13 0.7008043616996176 1102 14 0.8778612826316845 1102 15 0.7664369355265634 1102 16 0.762858596460255 1102 17 0.8439770305945302 1102 18 0.8448851735562997 1102 19 0.8622079060655139 1102 20 0.7737862293412547 1102 21 0.8213764998845434 1102 22 0.8477974254429199 1102 23 0.7470593814809448 1102 24 0.8522417520757983 1102 25 0.8653610184810034 1103 1 0.4663404994456606 1103 2 0.4918621801056915 1103 3 0.479430092110281 1103 4 0.5348349015579643 1103 5 0.5152996818083722 1103 6 0.43828687495654606 1103 7 0.5095117060724803 1103 8 0.6173364098979661 1103 9 0.6121507710945266 1103 10 0.5021258653207639 1103 11 0.6496031887995812 1103 12 0.5616482102412235 1103 13 0.7200331358443819 1103 14 0.7662222665043998 1103 15 0.7260255330126597 1103 16 0.678837882733454 1103 17 0.7399591302771427 1103 18 0.7662386579345508 1103 19 0.6809496087057241 1103 20 0.8549856209414699 1103 21 0.7673939137165533 1103 22 0.8213953364864324 1103 23 0.7434245151325456 1103 24 0.9168719206166074 1103 25 0.9454529231884834 1104 1 0.18001655055764942 1104 2 0.27577083250243817 1104 3 0.3060402897711292 1104 4 0.42551674257897076 1104 5 0.4269622128257424 1104 6 0.40060164038406754 1104 7 0.348057310302444 1104 8 0.37490677391240723 1104 9 0.38157981958597986 1104 10 0.3834516694533221 1104 11 0.4194245442103224 1104 12 0.4168355512892816 1104 13 0.4415634420370367 1104 14 0.5212849583096286 1104 15 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2214 8 -0.5448494764134466 2214 9 -0.529906365090838 2214 10 -0.6493901063818015 2214 11 -0.640896049632282 2214 12 -0.6866951800372405 2214 13 -0.650562373291784 2214 14 -0.7126756621363981 2214 15 -0.6478157954593726 2214 16 -0.7748805334170967 2214 17 -0.7843436501099603 2214 18 -0.7564995908302237 2214 19 -0.7712042758225053 2214 20 -0.820025921990279 2214 21 -0.8062107183160094 2214 22 -0.7626239388828111 2214 23 -0.8608800696668766 2214 24 -0.7806220690982428 2214 25 -0.6962295320859724 2215 1 -0.37826333603910456 2215 2 -0.29747922609941213 2215 3 -0.4230934639235642 2215 4 -0.188565281979713 2215 5 -0.32947393473718734 2215 6 -0.37084368929121325 2215 7 -0.43672046038609125 2215 8 -0.5586253780446171 2215 9 -0.6566497387710584 2215 10 -0.7192440849986852 2215 11 -0.5671441891713727 2215 12 -0.6035752303414981 2215 13 -0.6830922287495582 2215 14 -0.7827967472330641 2215 15 -0.9017910477186675 2215 16 -0.8126401528213094 2215 17 -0.8566020631705271 2215 18 -0.7786357824409507 2215 19 -0.7909825345929775 2215 20 -0.7703742713738038 2215 21 -0.8577141802254857 2215 22 -0.8891801951894891 2215 23 -0.819535700376214 2215 24 -0.8474169405268626 2215 25 -0.8662400290690826 2216 1 -0.4840267271799945 2216 2 -0.4271946636184296 2216 3 -0.556003087431499 2216 4 -0.38019477232506915 2216 5 -0.5771053814827667 2216 6 -0.6396849496422163 2216 7 -0.764860311751562 2216 8 -0.565062836917761 2216 9 -0.7964906382670378 2216 10 -0.697099261853019 2216 11 -0.6130520815186529 2216 12 -0.7513008413775282 2216 13 -0.8464531215001472 2216 14 -1.1117638528220724 2216 15 -0.9893210894882216 2216 16 -0.9599037868907416 2216 17 -1.134714555593753 2216 18 -1.108909218558999 2216 19 -1.1607978971107542 2216 20 -1.094031000376404 2216 21 -1.2474188746426123 2216 22 -1.185049614024237 2216 23 -1.1823503576936716 2216 24 -1.1689294713941152 2216 25 -1.2725949099279674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48611.0 3 0.0 4 812.0 5 6601.0 6 7633.0 7 5885.0 8 4579.0 9 4165.0 10 3776.0 11 3530.0 12 3178.0 13 2982.0 14 3035.0 15 3089.0 16 3141.0 17 3332.0 18 3629.0 19 4525.0 20 5478.0 21 7340.0 22 9200.0 23 11422.0 24 13260.0 25 14677.0 26 16235.0 27 18368.0 28 20732.0 29 24944.0 30 31345.0 31 40586.0 32 50980.0 33 66896.0 34 88979.0 35 119549.0 36 172199.0 37 278312.0 38 1058664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.43409608194731 18.84492549633194 12.95730273131453 27.76367569040622 2 14.2816496104521 20.44631456224439 38.90093824601662 26.371097581286897 3 16.97995891855882 24.523769869908772 29.832852199337516 28.663419012194897 4 11.399176821468703 16.993867102512418 37.31440432897349 34.292551747045394 5 13.010323014948163 37.86532995729956 35.935360898102765 13.188986129649512 6 32.50121607973027 37.1782494393037 17.66207279397567 12.65846168699036 7 28.091596130032965 31.362040339612374 22.342498005600973 18.203865524753684 8 25.637438134644736 36.160736799782725 20.307659991662607 17.894165073909925 9 26.516198038636947 14.616771791457827 19.400682285647665 39.46634788425756 10 14.127562994342572 29.005548248667758 34.927689568246976 21.939199188742695 11 34.94845880659805 22.078819652777554 23.95246450774841 19.020257032875985 12 22.705603864421427 24.734221566761608 31.63518559039335 20.924988978423617 13 28.8861985808188 21.336892928565845 25.56594002134462 24.210968469270735 14 21.24627191227365 21.025512680008752 27.555443089037862 30.172772318679737 15 23.667314468588856 29.506876399670812 23.12819400194942 23.69761512979092 16 23.089103835971187 27.708913806877927 26.31744267970721 22.88453967744368 17 22.458697207243638 27.878980249246492 27.069396465167582 22.59292607834228 18 22.448910653857563 27.34297455840101 27.97118275370454 22.23693203403689 19 23.578632991452437 26.972353306634826 27.125799555806186 22.32321414610655 20 23.801655110009904 26.795915563391066 27.19519038298648 22.207238943612552 21 23.543564909735533 26.399210232193216 26.80607573480974 23.25114912326151 22 23.464970816531117 27.03896850072791 26.610086928202236 22.88597375453874 23 22.91947907751382 26.770003007101383 26.88672480396012 23.423793111424672 24 23.055657457270286 26.798367372618102 27.22123507345482 22.924740096656794 25 23.19810109249171 26.58946996387968 26.95539177226433 23.25703717136428 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 112.0 1 112.0 2 202.0 3 292.0 4 292.0 5 292.0 6 740.5 7 1189.0 8 1189.0 9 1189.0 10 1828.5 11 2468.0 12 2468.0 13 2468.0 14 4746.0 15 7024.0 16 7024.0 17 7024.0 18 13958.5 19 20893.0 20 20893.0 21 20893.0 22 33843.0 23 46793.0 24 46793.0 25 46793.0 26 67526.0 27 88259.0 28 88259.0 29 88259.0 30 113368.5 31 138478.0 32 138478.0 33 138478.0 34 163523.5 35 188569.0 36 188569.0 37 188569.0 38 211121.5 39 233674.0 40 233674.0 41 233674.0 42 250263.5 43 266853.0 44 266853.0 45 266853.0 46 279874.0 47 292895.0 48 292895.0 49 292895.0 50 285824.0 51 278753.0 52 278753.0 53 278753.0 54 252131.0 55 225509.0 56 225509.0 57 225509.0 58 195364.0 59 165219.0 60 165219.0 61 165219.0 62 134773.0 63 104327.0 64 104327.0 65 104327.0 66 80652.0 67 56977.0 68 56977.0 69 56977.0 70 41754.5 71 26532.0 72 26532.0 73 26532.0 74 18638.0 75 10744.0 76 10744.0 77 10744.0 78 7404.5 79 4065.0 80 4065.0 81 4065.0 82 2738.0 83 1411.0 84 1411.0 85 1411.0 86 952.5 87 494.0 88 494.0 89 494.0 90 309.5 91 125.0 92 125.0 93 125.0 94 68.5 95 12.0 96 12.0 97 12.0 98 7.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1845796002995833 2 0.008743244224717105 3 0.005042400108434732 4 0.0022205064697694236 5 0.08040083842623454 6 0.33330727322268117 7 0.0285427602468278 8 0.01489589756803655 9 0.005134921211341792 10 0.00397840742500355 11 0.0 12 0.0 13 0.0 14 0.001757900955234127 15 0.0 16 0.0 17 0.0201233398822854 18 0.027848851975024857 19 0.0 20 0.0 21 9.714715805241227E-4 22 0.0 23 0.005505005622970029 24 0.0 25 2.3130275726764826E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2161669.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.64448773331974 #Duplication Level Percentage of deduplicated Percentage of total 1 82.56001452235215 60.800899767540606 2 11.999656442091114 17.674171033072604 3 2.9946364441809146 6.616154006377006 4 1.0650306965451808 3.1373456026932214 5 0.47611183674287894 1.7531506160349635 6 0.2558393842396258 1.130469623660112 7 0.1569057618206485 0.8088671116181554 8 0.10324932972429345 0.6082995197083371 9 0.07015995540394379 0.4650204577604398 >10 0.29343832458555696 3.690606239464374 >50 0.014896756910069978 0.7550461154988187 >100 0.008301663692427895 1.208211562547361 >500 0.0011935268755696763 0.6218458473777829 >1k 5.653548357955769E-4 0.7299124966462751 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2626 0.12148020811696889 No Hit GTATCAACGCAGAGTACTTTTTTTT 2535 0.11727049793469768 No Hit CCCATGTACTCTGCGTTGATACCAC 2522 0.1166691107658018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.626055145352966E-5 0.0 5 0.0 0.0 0.0 4.626055145352966E-5 0.0 6 0.0 0.0 0.0 4.626055145352966E-5 0.0 7 0.0 0.0 0.0 4.626055145352966E-5 0.0 8 0.0 0.0 0.0 4.626055145352966E-5 0.0 9 0.0 0.0 0.0 2.313027572676483E-4 4.626055145352966E-5 10 0.0 0.0 0.0 2.7756330872117795E-4 4.626055145352966E-5 11 0.0 0.0 0.0 2.7756330872117795E-4 4.626055145352966E-5 12 0.0 0.0 0.0 2.7756330872117795E-4 2.313027572676483E-4 13 4.626055145352966E-5 0.0 0.0 2.7756330872117795E-4 2.313027572676483E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAC 125 1.8559149E-8 11.390392 13 GTCCTAC 1015 0.0 10.509185 1 TACACCG 100 2.3475892E-5 10.470753 5 ACTATAC 265 0.0 10.416872 3 GTATTAG 220 0.0 10.389763 1 AGGACCG 110 5.9060785E-6 10.384217 5 TGGCGAG 800 0.0 10.322065 18 CCTAGAC 250 0.0 10.2803755 3 CCTATAC 215 1.8189894E-12 10.182974 3 TATGGCG 890 0.0 10.02502 16 GGCGAGA 525 0.0 9.941003 19 GTCCTAA 585 0.0 9.930956 1 CGTTGAT 615 0.0 9.877847 14 TCGAACT 165 1.0417352E-8 9.77669 19 GCGTTGA 615 0.0 9.723505 13 ACTGTGC 470 0.0 9.6914835 8 TCCTACA 1220 0.0 9.676035 2 GAACTGT 335 0.0 9.659818 6 ATGGCGA 900 0.0 9.597682 17 CTGCGTT 665 0.0 9.562476 11 >>END_MODULE