FastQCFastQC Report
Thu 26 May 2016
SRR1513152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061378
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC27420.13301781623748773No Hit
CCCATGTACTCTGCGTTGATACCAC23270.11288565221904959No Hit
GTCCTACAGTGGACATTTCTAAATT21420.10391107307830005No Hit
GTCCTAAAGTGTGTATTTCTCATTT21150.10260126963613661No Hit
GTATCAACGCAGAGTACTTTTTTTT20820.10100039876238127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG350.002185007816.26950517
TACCGTA500.001515838413.2812817
TCCAACG655.5066124E-513.14043518
CGCAGAT802.0327352E-613.0437988
TAGACCG550.003050085212.1009075
TAGGACC6000.012.0682334
GCGTGCG658.099276E-411.6795379
GTGCTAG2100.011.3711441
CGCTCGC600.00592154611.07203318
ATACCGT600.00594512411.0661236
AAGAGCG951.3482675E-511.0090965
GTCCTAC12850.011.0012511
GGCGAGG6000.010.91412619
GCGTTTA700.001504507310.84607213
GTACCGT700.001511222910.8402836
TGTAGGA13500.010.8057932
GTAGGAC13550.010.7585723
TGGCGAG13000.010.65835218
GGACGTG11700.010.4580946
TCCTACA14000.010.3517682
Produced by FastQC (version 0.11.5)