##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513152_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2061378
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.56290743376518	31.0	30.0	34.0	25.0	34.0
2	29.433019077529693	31.0	30.0	34.0	23.0	34.0
3	29.604884208524588	31.0	30.0	34.0	24.0	34.0
4	32.67117481606964	37.0	33.0	37.0	23.0	37.0
5	32.82831387547553	37.0	35.0	37.0	25.0	37.0
6	33.171026371679524	37.0	35.0	37.0	27.0	37.0
7	33.1452615677474	37.0	35.0	37.0	27.0	37.0
8	33.020362107289394	37.0	35.0	37.0	26.0	37.0
9	34.267262481699134	39.0	35.0	39.0	25.0	39.0
10	33.85336895998696	39.0	34.0	39.0	19.0	39.0
11	34.313604782820036	39.0	35.0	39.0	27.0	39.0
12	34.30543937113911	39.0	35.0	39.0	26.0	39.0
13	34.35313125491783	39.0	35.0	39.0	26.0	39.0
14	34.58838408094003	40.0	34.0	41.0	17.0	41.0
15	35.02684854500242	40.0	34.0	41.0	23.0	41.0
16	34.87784482030952	40.0	34.0	41.0	19.0	41.0
17	34.494960167421986	39.0	34.0	41.0	17.0	41.0
18	34.235481313956	39.0	33.0	41.0	17.0	41.0
19	34.86292373354135	40.0	34.0	41.0	21.0	41.0
20	35.07683258480492	40.0	35.0	41.0	24.0	41.0
21	35.12381911517441	40.0	36.0	41.0	24.0	41.0
22	34.81288439092685	40.0	35.0	41.0	18.0	41.0
23	34.36150332447518	40.0	34.0	41.0	15.0	41.0
24	34.612512600794226	40.0	34.0	41.0	18.0	41.0
25	34.2617802266251	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7314774545222171
1101	2	0.993431462119684
1101	3	0.9000643447619261
1101	4	1.2982671635475285
1101	5	1.0876205295449708
1101	6	0.8394301553056422
1101	7	0.7586895225672521
1101	8	0.8239968039623449
1101	9	1.2154972848374115
1101	10	1.5476766948988967
1101	11	1.0207866145667452
1101	12	0.9436301647257324
1101	13	0.8392057578626648
1101	14	1.780618118768011
1101	15	1.3972757150729223
1101	16	1.529273515974424
1101	17	1.825745232254107
1101	18	2.0060180582532112
1101	19	1.5791388816120886
1101	20	1.224789026208974
1101	21	1.2532292217551984
1101	22	1.4155958270455429
1101	23	1.7193269896955385
1101	24	1.2791454552222703
1101	25	1.5715718352761385
1102	1	0.8859685537489419
1102	2	1.1775696266802314
1102	3	1.0363263493760186
1102	4	1.2194632704226862
1102	5	1.2344519965552578
1102	6	0.9183506524471952
1102	7	0.9328300455593279
1102	8	0.9934184875662382
1102	9	1.2469131738145762
1102	10	1.4365491142939035
1102	11	1.1272289535510964
1102	12	0.9721289769458323
1102	13	1.0577631883054082
1102	14	1.9849123087258391
1102	15	1.537545076939324
1102	16	1.6406883245527908
1102	17	1.942160179176227
1102	18	2.1837660591860413
1102	19	1.601272563835174
1102	20	1.308499510952295
1102	21	1.3119831530233839
1102	22	1.5253228418904854
1102	23	1.823249496115345
1102	24	1.5543981301974625
1102	25	1.8420878838097607
1103	1	0.9081940277494667
1103	2	1.1936085386222928
1103	3	0.9792216767859472
1103	4	1.4656806016257846
1103	5	1.279324168288774
1103	6	1.0368213346509023
1103	7	1.0386081325790641
1103	8	0.9425172819748155
1103	9	1.3789325733640254
1103	10	1.6119170874647892
1103	11	1.257555490237074
1103	12	1.1711584826346169
1103	13	1.2455359419780336
1103	14	2.102516747338619
1103	15	1.706345103987232
1103	16	1.682193505970794
1103	17	2.109161283704097
1103	18	2.335419846872483
1103	19	1.7118397019666247
1103	20	1.4727090391795628
1103	21	1.5238853938001284
1103	22	1.6962254343330514
1103	23	2.0248584587916056
1103	24	1.6889322741926662
1103	25	1.85211337473676
1104	1	0.7422372929646137
1104	2	1.02831389559568
1104	3	0.8736962962661003
1104	4	1.2702879289371651
1104	5	1.1093968976475281
1104	6	0.9511790658817318
1104	7	0.7762981724774107
1104	8	0.8433003295416128
1104	9	1.112435869030726
1104	10	1.3263553427957504
1104	11	0.9770681211430841
1104	12	0.9072637013576497
1104	13	0.8058340614105219
1104	14	1.7920631519226973
1104	15	1.3062635407296312
1104	16	1.4740153791115134
1104	17	1.8544340980616951
1104	18	1.9470008115011552
1104	19	1.4011309827886507
1104	20	1.1061860059891941
1104	21	1.0955278256533987
1104	22	1.3456231418456426
1104	23	1.712653883041959
1104	24	1.465473760911017
1104	25	1.748546806044267
1105	1	0.7334086645892839
1105	2	0.9770295409492142
1105	3	0.934714647318895
1105	4	1.2201271489705547
1105	5	1.107606065570394
1105	6	0.8653877308340583
1105	7	0.829355034897489
1105	8	0.9084582881973517
1105	9	1.0932717015954552
1105	10	1.4087425698715705
1105	11	0.9415183404344418
1105	12	0.977404501514691
1105	13	0.9361758854959419
1105	14	1.775338854970414
1105	15	1.4430897460844179
1105	16	1.4200368252866085
1105	17	1.806516360283318
1105	18	1.37942372884401
1105	19	1.3244927426631818
1105	20	1.2429235385648525
1105	21	1.1412846646731296
1105	22	1.3339376071140308
1105	23	1.6364448949921453
1105	24	1.4257072841501284
1105	25	1.641355949155603
1106	1	0.5802597939180707
1106	2	0.7582388903436303
1106	3	0.6595211186556469
1106	4	1.190027622325431
1106	5	1.0444100428016796
1106	6	0.6785940692963379
1106	7	0.650413179147975
1106	8	0.6997224170505731
1106	9	0.764134597900437
1106	10	0.6360387703202974
1106	11	0.6165992092200767
1106	12	0.6158004716004299
1106	13	0.5092691389489872
1106	14	1.4853697103560606
1106	15	1.1072474415709763
1106	16	1.2042031317723243
1106	17	1.5269062559885285
1106	18	1.5936408802371318
1106	19	0.9486290898740108
1106	20	0.8463113790504693
1106	21	0.7713624345827341
1106	22	0.9546003789638178
1106	23	1.3693438716336601
1106	24	1.12502940762419
1106	25	1.3478134066101362
1107	1	0.6096199696111704
1107	2	0.7944848703635436
1107	3	0.7657276613194739
1107	4	1.1346224938555025
1107	5	0.936515632933947
1107	6	0.6510502468741706
1107	7	0.6970282066524689
1107	8	0.7182643482869295
1107	9	0.986705949017157
1107	10	1.142823117046298
1107	11	0.6595694278843993
1107	12	0.8137642195692578
1107	13	0.7080725847153673
1107	14	1.5290848254811849
1107	15	0.9956521488809287
1107	16	1.1984468752875088
1107	17	1.5858367839553296
1107	18	1.694811051460448
1107	19	1.237843360258715
1107	20	1.07711773905028
1107	21	0.9339384571865992
1107	22	1.2486910687464103
1107	23	1.4730270851004548
1107	24	1.2793290718257495
1107	25	1.5152745219256758
1108	1	0.5351082479927598
1108	2	0.7657325513240423
1108	3	0.5224847291803059
1108	4	0.9954967945713165
1108	5	0.9769758764400436
1108	6	0.5551016495705667
1108	7	0.6114881969198436
1108	8	0.5743261210690846
1108	9	0.8066998601740281
1108	10	1.0804534575848947
1108	11	0.7251198529523322
1108	12	0.489604769676788
1108	13	0.4279720076645077
1108	14	1.3697810017353333
1108	15	0.936146607398598
1108	16	1.0198736889014342
1108	17	1.340748344898138
1108	18	1.511040815186341
1108	19	0.9489662556584264
1108	20	0.7435466801869595
1108	21	0.7294778635918675
1108	22	0.904639677957789
1108	23	1.2110125353850876
1108	24	0.9291220551286656
1108	25	1.39406407261351
1109	1	0.4175572421015552
1109	2	0.7099744750001982
1109	3	0.5295037879115476
1109	4	0.869443999979012
1109	5	0.901058481729379
1109	6	0.6306979573674454
1109	7	0.4766372257060283
1109	8	0.5186848215858859
1109	9	0.7782660826210659
1109	10	1.1878163263038175
1109	11	0.7454752813464935
1109	12	0.594308742361676
1109	13	0.5422029748672088
1109	14	1.4643611534266725
1109	15	0.9442786027709928
1109	16	1.0822063408718705
1109	17	1.4414604662749184
1109	18	1.5974695086985093
1109	19	1.068929958017769
1109	20	0.9204712998471649
1109	21	0.9025433149363309
1109	22	1.215300356271186
1109	23	1.4356404944479877
1109	24	1.2103526972926133
1109	25	1.3801032136022826
1110	1	0.47922437574970544
1110	2	0.698262384215095
1110	3	0.6520916007688022
1110	4	0.9696276359716691
1110	5	0.8440924685439057
1110	6	0.5349463824032483
1110	7	0.6667827774877679
1110	8	0.6090626275018778
1110	9	0.7407364895002431
1110	10	1.1007243452707485
1110	11	0.5465514214565559
1110	12	0.5175457733747777
1110	13	0.36960788881407325
1110	14	1.194867670503065
1110	15	0.9949657489348951
1110	16	1.1715025395775314
1110	17	1.283469567044314
1110	18	0.959900181474012
1110	19	0.8047744151103089
1110	20	0.7330643747884125
1110	21	0.5755216837683932
1110	22	0.8311292711786962
1110	23	-0.39824605736401253
1110	24	0.3949981509594309
1110	25	0.807086672695192
1111	1	0.23461830987633903
1111	2	0.5561721372684332
1111	3	0.43070764687001883
1111	4	0.8038572584660457
1111	5	0.7201190148877501
1111	6	0.41392363839585045
1111	7	0.4433335392828539
1111	8	0.47320840545991416
1111	9	0.636401729385426
1111	10	0.8920786771579898
1111	11	0.5734109329710009
1111	12	0.43292068374827863
1111	13	0.3291782610084937
1111	14	1.16516550812036
1111	15	0.8216721047515065
1111	16	0.842066854589639
1111	17	1.2560812531330754
1111	18	1.5957976173714457
1111	19	0.9825199072292108
1111	20	0.6583712293749997
1111	21	0.4809069026984787
1111	22	0.828130664161634
1111	23	1.181888702267969
1111	24	0.8983612511552224
1111	25	1.267154318071185
1112	1	0.2500646496680119
1112	2	0.362284776885371
1112	3	0.32510433757445156
1112	4	0.8006717236016385
1112	5	0.43914196178263154
1112	6	0.3180299451718014
1112	7	0.15936363958501687
1112	8	0.3040650766638322
1112	9	0.6127681653281556
1112	10	0.7672898523054528
1112	11	0.35698599547884413
1112	12	0.4266830284440388
1112	13	0.2881191512746497
1112	14	0.8437337647548802
1112	15	0.6901518367642083
1112	16	0.6303748469052763
1112	17	1.129108080823812
1112	18	1.135108534764555
1112	19	0.6096629850285495
1112	20	0.392824973570697
1112	21	0.4837645971968243
1112	22	0.5756215655295662
1112	23	0.9840688020874495
1112	24	0.74274201321996
1112	25	1.0800967722401467
1113	1	-0.3134778227919277
1113	2	-0.16097670425950028
1113	3	-0.2088374576048011
1113	4	0.13931881187668438
1113	5	0.01214553126614959
1113	6	-0.2987620446312178
1113	7	-0.2427206019218957
1113	8	-0.1777039047903486
1113	9	-0.05953746432665241
1113	10	0.248408706740598
1113	11	-0.2509363423343629
1113	12	-0.3648506160868976
1113	13	-0.5161353793793211
1113	14	0.14292025321331892
1113	15	-0.16140866079735616
1113	16	-0.0544470046086758
1113	17	0.21934642411477512
1113	18	0.21659613176327497
1113	19	-0.10545717524939136
1113	20	-0.3161399378782832
1113	21	-0.3549875212324807
1113	22	-0.17531301012741096
1113	23	-0.062156125094858794
1113	24	-0.17694065695876304
1113	25	0.23363226067661458
1114	1	-0.7722196856838934
1114	2	-0.43023021491390523
1114	3	-0.44545268382911374
1114	4	-0.047768059038546085
1114	5	-3.1733640370170413
1114	6	-1.0482054256041309
1114	7	-0.7532239693624518
1114	8	-0.38500810736857005
1114	9	-0.1905751089632446
1114	10	-4.935396891170285E-4
1114	11	-0.2786839735372837
1114	12	-0.47571059189632336
1114	13	-0.5021897571272262
1114	14	-5.871956693778429
1114	15	-2.289306174285308
1114	16	-1.114976721518481
1114	17	-0.040048710119961584
1114	18	-5.980252590139436
1114	19	-2.1175544341620878
1114	20	-1.152029597372838
1114	21	-0.8901170922920727
1114	22	-0.6324727770114436
1114	23	-0.09195485124111968
1114	24	-0.23871900129477552
1114	25	-0.15784133867779815
1115	1	-1.058034410172212
1115	2	-0.6686070412780509
1115	3	-0.6286344116874574
1115	4	-3.4281366041059442
1115	5	-1.356492117215808
1115	6	-0.964017804128737
1115	7	-0.8748618736767355
1115	8	-0.7429229538200843
1115	9	-0.5496507364636827
1115	10	-0.8288286545493833
1115	11	-0.8539926830733364
1115	12	-0.9424535734053805
1115	13	-0.962172774682486
1115	14	-6.3860854508527645
1115	15	-2.715178341242563
1115	16	-1.540099794901387
1115	17	-0.7590067433859957
1115	18	-0.490957675587687
1115	19	-0.8538480178392049
1115	20	-0.9356855418340544
1115	21	-1.026822363443756
1115	22	-1.9972874481212344
1115	23	-1.2782498478791666
1115	24	-1.048171216525958
1115	25	-0.6079595564092486
1116	1	-0.5321178305014271
1116	2	-0.23116985473698293
1116	3	-0.4355007998050766
1116	4	-0.2310407658752922
1116	5	-0.7086650674590302
1116	6	-0.4148180445537122
1116	7	-0.39852386081675917
1116	8	-0.27014370110050123
1116	9	-0.24684310159167921
1116	10	-0.1324925397813317
1116	11	-0.3721931945847814
1116	12	-0.6614327892987362
1116	13	-0.6050561872246547
1116	14	0.29537635572518184
1116	15	-0.15079076384867562
1116	16	-0.0800148025627081
1116	17	0.24098757856122432
1116	18	0.0943149155592522
1116	19	-0.15810331282621348
1116	20	-0.6167160597454711
1116	21	-0.7259449420584261
1116	22	-0.57461303659057
1116	23	-1.1665089325608378
1116	24	-0.8222269766266024
1116	25	-0.33911389642889134
1201	1	1.132892014875143
1201	2	1.2368087836263442
1201	3	1.1470718199881524
1201	4	1.670958705528406
1201	5	1.4368927224315087
1201	6	1.156539752043436
1201	7	1.0902872770607033
1201	8	1.3288925661586575
1201	9	1.4595980931513495
1201	10	1.7266641291677445
1201	11	1.2425082368439107
1201	12	1.2694950702864745
1201	13	1.134677199761569
1201	14	2.022821501238802
1201	15	1.6444329755338742
1201	16	1.747620255415221
1201	17	2.029530127498745
1201	18	1.4868459868177126
1201	19	1.4171039340632348
1201	20	1.4990124566548957
1201	21	1.3386276395143284
1201	22	1.6508843072670913
1201	23	1.8695710102382677
1201	24	1.51309003512403
1201	25	1.8117181379493346
1202	1	1.1455155985741179
1202	2	1.252934333625845
1202	3	1.1521580772414062
1202	4	1.7053087394389053
1202	5	1.4150879846229216
1202	6	1.2004471230908393
1202	7	1.0088254834208712
1202	8	1.2337928400487144
1202	9	1.491248529178307
1202	10	1.678994186286964
1202	11	1.3029323840017142
1202	12	1.2236855966609212
1202	13	1.2018390593155246
1202	14	2.2540208778195137
1202	15	1.7516537192257928
1202	16	1.8563817498671824
1202	17	2.29560848247187
1202	18	2.4225804505434354
1202	19	1.928416332932926
1202	20	1.6963051465425778
1202	21	1.5216531299135596
1202	22	1.742185760704409
1202	23	2.1095058630925436
1202	24	1.6787129024756027
1202	25	2.2711895282290016
1203	1	0.731292572536745
1203	2	0.8723862135000147
1203	3	0.6571268149326386
1203	4	1.1239249684500052
1203	5	1.0687623502835706
1203	6	0.7370527853808468
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2211	18	-0.6641309175953722
2211	19	-0.7170287471625656
2211	20	-0.8436105600999326
2211	21	-0.791731961458602
2211	22	-0.6363440915870271
2211	23	-0.22588840280462108
2211	24	-0.6266014137070144
2211	25	-7.712957509273089
2212	1	-1.2579521622871574
2212	2	-3.574850636367561
2212	3	-1.5164642866636022
2212	4	-3.3214279683430448
2212	5	-1.0348658706877103
2212	6	-0.8208653160197841
2212	7	-0.7451269221243635
2212	8	-0.6893027217237986
2212	9	-0.7340111818567365
2212	10	-0.19200342291248518
2212	11	-0.7954317471966519
2212	12	-0.841067020713723
2212	13	-1.0887242648622077
2212	14	-6.345204099831864
2212	15	-4.834664640918167
2212	16	-2.5840554146140278
2212	17	-7.354272498582365
2212	18	-2.926969527764122
2212	19	-1.7217873328583195
2212	20	-1.7531531020667117
2212	21	-1.2256925372332717
2212	22	-1.177504048942474
2212	23	-0.5599151069379218
2212	24	-0.898725837602619
2212	25	-0.7659329190490993
2213	1	-0.2955517370758969
2213	2	-0.17554829728070231
2213	3	-0.29292791566182075
2213	4	0.054699507216511734
2213	5	-0.031594810549073316
2213	6	-0.3732373347959381
2213	7	-0.37041534198719717
2213	8	-0.4545567507492123
2213	9	-0.22395295964206952
2213	10	-3.914253678577481
2213	11	-1.550444657924949
2213	12	-1.01505926861104
2213	13	-0.7911040031632339
2213	14	-0.008102009469517668
2213	15	-0.5223231036420657
2213	16	-0.4460400478020361
2213	17	-0.0037177197007167706
2213	18	0.15393852915356376
2213	19	-0.4410963787250495
2213	20	-0.7633168480617698
2213	21	-0.7488916570657977
2213	22	-0.8818237605013692
2213	23	-1.178524367251626
2213	24	-0.6517162682246251
2213	25	-0.4156162768866096
2214	1	-0.8986677588614427
2214	2	-0.6944045180130018
2214	3	-0.6546958485327963
2214	4	-0.2635738971578476
2214	5	-3.4956128379116116
2214	6	-1.4235873953017162
2214	7	-1.2047403604091542
2214	8	-0.8833281248107454
2214	9	-0.6876108624108568
2214	10	-4.281738914162016
2214	11	-1.7738168306697375
2214	12	-1.3098928751124745
2214	13	-1.0363481586303536
2214	14	-0.2549028940006508
2214	15	-0.6038912375176508
2214	16	-0.6058950071455413
2214	17	-0.2336405570737199
2214	18	0.12322106188090487
2214	19	-0.6157838290325302
2214	20	-0.7885079011741709
2214	21	-0.93027666264868
2214	22	-0.6703292902067091
2214	23	-0.45119134363584124
2214	24	-1.0867660049127466
2214	25	-0.7051874566644756
2215	1	-2.279033953848451
2215	2	-4.733554672179171
2215	3	-2.859109053278665
2215	4	-1.9870410810529435
2215	5	-1.9570816615624764
2215	6	-2.0354962714864477
2215	7	-2.295552587812608
2215	8	-2.4703034607936196
2215	9	-8.143886783445566
2215	10	-3.7037513930974946
2215	11	-3.1419892054970013
2215	12	-3.4718047069698272
2215	13	-2.9262434421478964
2215	14	-8.209170242728039
2215	15	-4.851214726033792
2215	16	-9.294628754158815
2215	17	-4.554028272784272
2215	18	-3.0231384490023956
2215	19	-3.4855255375141105
2215	20	-3.320577548815155
2215	21	-3.5233501937864773
2215	22	-9.072212781875567
2215	23	-10.295743267950058
2215	24	-6.5518626811089575
2215	25	-4.986669782170562
2216	1	-2.0253487491566737
2216	2	-1.9335104720631477
2216	3	-1.836904260208474
2216	4	-6.937982475462512
2216	5	-3.5689943134853586
2216	6	-2.6715277736778376
2216	7	-2.127666454357886
2216	8	-8.024749636142367
2216	9	-3.975500516069296
2216	10	-2.2911951970940017
2216	11	-2.2856416644687627
2216	12	-2.519262259444453
2216	13	-2.254634369753788
2216	14	-1.6568082790097662
2216	15	-2.246975598601736
2216	16	-1.9552566600461958
2216	17	-1.616135521323045
2216	18	-7.668189257115838
2216	19	-4.853762469353448
2216	20	-4.0193651284224075
2216	21	-3.290706886720983
2216	22	-2.8899740219770855
2216	23	-2.207402049460157
2216	24	-2.5480794074097517
2216	25	-2.005282995523004
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91504.0
3	1.0
4	757.0
5	4553.0
6	5357.0
7	4959.0
8	4634.0
9	4232.0
10	4114.0
11	3927.0
12	3732.0
13	3764.0
14	3779.0
15	3716.0
16	3927.0
17	4235.0
18	4755.0
19	5728.0
20	7438.0
21	9533.0
22	11767.0
23	14067.0
24	15974.0
25	18628.0
26	21021.0
27	23594.0
28	26681.0
29	31642.0
30	38193.0
31	45361.0
32	58004.0
33	76107.0
34	100842.0
35	134773.0
36	193130.0
37	284008.0
38	792941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11005849533174	18.88226198794718	12.383487970020536	25.624191546700537
2	14.188787604946581	20.491127144042498	41.66424292063504	23.655842330375876
3	18.201812729965493	25.213629277996514	30.031055220900626	26.55350277113737
4	11.230581930436596	17.005291871727916	39.69478893434433	32.06933726349116
5	12.382258601864212	38.0753321222998	36.81109092721883	12.73131834861716
6	31.196855108192995	37.740302854012604	18.548583134440534	12.51425890335387
7	27.534194731658086	31.687781114901092	22.958578159040073	17.81944599440075
8	24.789255039490115	36.624936560599544	21.315347434428162	17.270460965482172
9	26.40032683147655	14.91765409138569	20.53288668952611	38.14913238761165
10	13.519548518667857	29.033566575398034	36.20977520549999	21.23710970043412
11	33.979357252861035	22.496732114940276	24.99885975799698	18.52505087420171
12	22.752933572334598	24.99915081439326	32.229505269318004	20.018410343954137
13	28.618774611015546	21.97451008460245	25.929159094824133	23.47755620955787
14	20.186902011096304	21.883227730478353	29.20049144388815	28.72937881453719
15	23.055072804190385	30.502099705560763	23.63545830751065	22.807369182738203
16	21.80834881557796	28.105793262664104	28.13543959789671	21.950418323861225
17	21.434617176580215	28.370331184966606	28.471132939897497	21.72391869855568
18	21.256602684019867	27.606680205656776	29.99056152840155	21.146155581921807
19	23.043825528627607	27.288762465984217	27.903181664667454	21.76423034072073
20	23.377295801138697	27.46634393987111	28.05869448017807	21.097665778812125
21	23.05304703434802	26.90422358458015	27.31001925127254	22.73271012979929
22	22.768545485694705	27.953744331972313	27.4949149729114	21.782795209421582
23	22.072158850210045	27.608177375423328	28.045200821639014	22.27446295272761
24	22.66878602196202	27.392100952496513	28.072996420554507	21.866116604986956
25	22.520400759978358	27.267887517379773	27.903241069890345	22.308470652751524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	198.0
2	326.0
3	454.0
4	454.0
5	454.0
6	901.0
7	1348.0
8	1348.0
9	1348.0
10	2194.5
11	3041.0
12	3041.0
13	3041.0
14	5899.5
15	8758.0
16	8758.0
17	8758.0
18	16450.0
19	24142.0
20	24142.0
21	24142.0
22	39066.0
23	53990.0
24	53990.0
25	53990.0
26	77525.5
27	101061.0
28	101061.0
29	101061.0
30	129183.5
31	157306.0
32	157306.0
33	157306.0
34	181267.0
35	205228.0
36	205228.0
37	205228.0
38	223255.5
39	241283.0
40	241283.0
41	241283.0
42	253660.5
43	266038.0
44	266038.0
45	266038.0
46	271772.0
47	277506.0
48	277506.0
49	277506.0
50	263203.5
51	248901.0
52	248901.0
53	248901.0
54	218529.0
55	188157.0
56	188157.0
57	188157.0
58	159844.0
59	131531.0
60	131531.0
61	131531.0
62	105440.5
63	79350.0
64	79350.0
65	79350.0
66	60700.0
67	42050.0
68	42050.0
69	42050.0
70	30560.0
71	19070.0
72	19070.0
73	19070.0
74	13371.5
75	7673.0
76	7673.0
77	7673.0
78	5252.0
79	2831.0
80	2831.0
81	2831.0
82	1929.5
83	1028.0
84	1028.0
85	1028.0
86	668.0
87	308.0
88	308.0
89	308.0
90	202.0
91	96.0
92	96.0
93	96.0
94	58.0
95	20.0
96	20.0
97	20.0
98	15.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16668461582494817
2	0.25866192420798123
3	0.26016577260453927
4	0.46337935109426803
5	0.2562848735166476
6	0.018676826860478765
7	0.01882236057627471
8	0.01727000094111803
9	0.0182887369516896
10	0.020811321358819197
11	0.019986630302642215
12	0.028136518387214766
13	0.027311827331037784
14	0.02483775416250683
15	0.04128306404744787
16	0.02013216401843815
17	0.04356309226158424
18	0.03216295119090239
19	0.027651406001228306
20	0.029349299352180922
21	0.03565576037000492
22	0.04448480579495852
23	0.030028456692561968
24	0.048656772314442084
25	0.03846941220872639
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2061378.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.4267105840924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.34716586922127	63.620292686061894
2	10.999051615699143	16.592445658336665
3	2.6182252612711507	5.924523570775764
4	0.9068603269516726	2.736059656847169
5	0.3949959573726472	1.4896622879316581
6	0.19774938843982312	0.8949351534019083
7	0.1161736616591302	0.6133818008820241
8	0.08245611821769787	0.4975515011755205
9	0.05766779929992954	0.3914723167035581
>10	0.2535069179640232	3.2807063818501154
>50	0.013646725108128685	0.6985659424000981
>100	0.010548523570009554	1.61614623367324
>500	0.0011711011352175701	0.6134187943802567
>1k	7.807340901450161E-4	1.0308380155801022
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2742	0.13301781623748773	No Hit
CCCATGTACTCTGCGTTGATACCAC	2327	0.11288565221904959	No Hit
GTCCTACAGTGGACATTTCTAAATT	2142	0.10391107307830005	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2115	0.10260126963613661	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2082	0.10100039876238127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.851123859864615E-5	0.0
10	0.0	0.0	0.0	4.851123859864615E-5	0.0
11	0.0	0.0	0.0	9.70224771972923E-5	0.0
12	0.0	0.0	0.0	9.70224771972923E-5	0.0
13	0.0	0.0	0.0	9.70224771972923E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTCG	35	0.0021850078	16.269505	17
TACCGTA	50	0.0015158384	13.281281	7
TCCAACG	65	5.5066124E-5	13.140435	18
CGCAGAT	80	2.0327352E-6	13.043798	8
TAGACCG	55	0.0030500852	12.100907	5
TAGGACC	600	0.0	12.068233	4
GCGTGCG	65	8.099276E-4	11.679537	9
GTGCTAG	210	0.0	11.371144	1
CGCTCGC	60	0.005921546	11.072033	18
ATACCGT	60	0.005945124	11.066123	6
AAGAGCG	95	1.3482675E-5	11.009096	5
GTCCTAC	1285	0.0	11.001251	1
GGCGAGG	600	0.0	10.914126	19
GCGTTTA	70	0.0015045073	10.846072	13
GTACCGT	70	0.0015112229	10.840283	6
TGTAGGA	1350	0.0	10.805793	2
GTAGGAC	1355	0.0	10.758572	3
TGGCGAG	1300	0.0	10.658352	18
GGACGTG	1170	0.0	10.458094	6
TCCTACA	1400	0.0	10.351768	2
>>END_MODULE