FastQCFastQC Report
Thu 26 May 2016
SRR1513152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061378
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31550.1530529577787286No Hit
CCCATGTACTCTGCGTTGATACCAC31060.15067590708739495No Hit
GTATCAACGCAGAGTACTTTTTTTT30180.14640691809071407No Hit
GTCCTAAAGTGTGTATTTCTCATTT22360.10847112950657278No Hit
GTCCTACAGTGGACATTTCTAAATT22300.10818006207498092No Hit
TATCAACGCAGAGTACTTTTTTTTT21670.10512385404326621No Hit
CTTTAGGACGTGAAATATGGCGAGG21630.10492980908887162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5100.012.6948764
GGCGAGG6300.012.64899219
GTCGAGG701.10554996E-412.19724219
CGTACTA658.1199274E-411.67599119
ACACCGT907.2930216E-611.6352696
TGGCGAG12650.011.02893618
ACCGTAC700.001502836510.84751817
CGTGCGC700.001509237710.84199210
GCGCCAC803.802833E-410.67802513
TATGTCG909.6035554E-510.54619916
GACGTGA7650.010.5441517
GTAGGAC11900.010.401773
TGTAGGA12600.010.2022322
TATGGCG14350.010.18608516
AGGACGT13550.010.1184245
CTACACT3300.010.0981964
TGTCGAG951.6604118E-49.99040818
ATGGCGA14450.09.984220517
TTAATAC2105.456968E-129.9750313
AAGGCGT1151.001606E-59.9336686
Produced by FastQC (version 0.11.5)