##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513152_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2061378 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.80408445224505 31.0 31.0 34.0 27.0 34.0 2 30.909191812467196 33.0 31.0 34.0 27.0 34.0 3 31.004106476347374 33.0 31.0 34.0 27.0 34.0 4 34.395791067916704 37.0 35.0 37.0 31.0 37.0 5 34.14095085908552 37.0 35.0 37.0 30.0 37.0 6 34.055087907215466 37.0 35.0 37.0 30.0 37.0 7 33.92243926150371 37.0 35.0 37.0 29.0 37.0 8 33.89141341374556 37.0 35.0 37.0 29.0 37.0 9 35.45297369041486 39.0 37.0 39.0 30.0 39.0 10 35.297930801628816 39.0 35.0 39.0 29.0 39.0 11 35.30885601767361 39.0 35.0 39.0 29.0 39.0 12 35.25120574683537 39.0 35.0 39.0 29.0 39.0 13 35.21754379837177 39.0 35.0 39.0 29.0 39.0 14 36.38959084651141 40.0 37.0 41.0 28.0 41.0 15 36.36900607263685 40.0 37.0 41.0 28.0 41.0 16 36.332746832458675 40.0 37.0 41.0 28.0 41.0 17 36.27348647361134 40.0 37.0 41.0 27.0 41.0 18 36.230901367919905 40.0 36.0 41.0 27.0 41.0 19 36.31056264304752 40.0 37.0 41.0 28.0 41.0 20 36.27825706881513 40.0 37.0 41.0 28.0 41.0 21 36.22894393944245 40.0 37.0 41.0 27.0 41.0 22 36.173061903251124 40.0 37.0 41.0 27.0 41.0 23 36.09665815779542 40.0 37.0 41.0 27.0 41.0 24 36.03373423020911 40.0 37.0 41.0 27.0 41.0 25 35.97816121060766 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3261997305133093 1101 2 0.41195012661752983 1101 3 0.4051803142764143 1101 4 0.5602173150736434 1101 5 0.3563809623017704 1101 6 0.41864886667959667 1101 7 0.3678427855195494 1101 8 0.4309842518865423 1101 9 0.42325544094361334 1101 10 0.567766326542916 1101 11 0.5078950303261252 1101 12 0.4741074719037357 1101 13 0.46964830928705226 1101 14 0.5249629136651777 1101 15 0.7241471534004233 1101 16 0.6303701550100271 1101 17 0.6010101993652484 1101 18 0.5761937352589683 1101 19 0.5497627301242503 1101 20 0.5558637738765384 1101 21 0.5451175452894859 1101 22 0.6380711841077726 1101 23 0.5897073912661384 1101 24 0.6082252553060243 1101 25 0.6333102991953012 1102 1 0.5143478874451901 1102 2 0.5926889876400345 1102 3 0.5088017583479889 1102 4 0.6192426636010708 1102 5 0.6085598763428735 1102 6 0.5977842577773771 1102 7 0.5758096726139215 1102 8 0.6627923783300886 1102 9 0.6104832825571478 1102 10 0.6885199969343248 1102 11 0.71048298071738 1102 12 0.7175924695123612 1102 13 0.7805561209656346 1102 14 0.7478715843925627 1102 15 0.6544113636091353 1102 16 0.7510368664737115 1102 17 0.914597268309187 1102 18 0.8262296387556418 1102 19 0.7102507014646946 1102 20 0.719413753520243 1102 21 0.7299184422840028 1102 22 0.862947629964637 1102 23 0.8908902961949963 1102 24 0.9389900063729186 1102 25 0.9122916675862598 1103 1 0.45716008992631885 1103 2 0.41623995969563055 1103 3 0.4689479850038296 1103 4 0.5244857361826973 1103 5 0.5134912304415025 1103 6 0.3140445275285373 1103 7 0.3394586890155864 1103 8 0.3954553930989775 1103 9 0.3449122713639241 1103 10 0.47303455488737 1103 11 0.5823032637599113 1103 12 0.3842245725752136 1103 13 0.5185081208415951 1103 14 0.6988984033802836 1103 15 0.7133105440533285 1103 16 0.6128601924054848 1103 17 0.7262284270642354 1103 18 0.6694076770633117 1103 19 0.729790981845511 1103 20 0.666981412582274 1103 21 0.6582828430210981 1103 22 0.5618031332117894 1103 23 0.6334942771899676 1103 24 0.7042373471827617 1103 25 0.7227101929887851 1104 1 0.21542990122486572 1104 2 0.2584870550119227 1104 3 0.2374383341469546 1104 4 0.3792777949413306 1104 5 0.30968424524620985 1104 6 0.1309636440707891 1104 7 0.18770999376791764 1104 8 0.2800132692392552 1104 9 0.2830737070214724 1104 10 0.34715557877134273 1104 11 0.4147816426572959 1104 12 0.3841426063790081 1104 13 0.44512748604998364 1104 14 0.3657175858454096 1104 15 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-0.9653068172937438 2215 18 -1.04269059269415 2215 19 -0.9174648436176724 2215 20 -0.8488365980728219 2215 21 -0.8893920791326124 2215 22 -1.000409681967966 2215 23 -0.8658408941773672 2215 24 -0.9098600123498315 2215 25 -0.8309136098233196 2216 1 -0.4615768069751205 2216 2 -0.4304500141069063 2216 3 -0.456526144854589 2216 4 -0.37429732536391924 2216 5 -0.6339740983801576 2216 6 -0.7073167643363263 2216 7 -0.8269652061816544 2216 8 -0.7934580700008809 2216 9 -1.0482733426284483 2216 10 -0.8340739961588142 2216 11 -0.8013025280391659 2216 12 -0.8208990026258576 2216 13 -0.8836001619512999 2216 14 -1.0513378968703009 2216 15 -1.1153749315131876 2216 16 -0.9751627593283629 2216 17 -1.2710341354076604 2216 18 -1.2480023736665657 2216 19 -1.1692150120705591 2216 20 -1.2623323668325526 2216 21 -1.4613372565756606 2216 22 -1.3752357140747051 2216 23 -1.2962844447438613 2216 24 -1.286938328629688 2216 25 -1.3224568544721649 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48430.0 3 1.0 4 843.0 5 6962.0 6 7682.0 7 5942.0 8 4687.0 9 4193.0 10 3778.0 11 3467.0 12 3148.0 13 3007.0 14 2850.0 15 2841.0 16 2994.0 17 3123.0 18 3517.0 19 4205.0 20 5298.0 21 6945.0 22 8715.0 23 10581.0 24 12269.0 25 13866.0 26 15049.0 27 16667.0 28 19085.0 29 22780.0 30 28925.0 31 36841.0 32 46895.0 33 61865.0 34 81936.0 35 110642.0 36 158796.0 37 259138.0 38 1033415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.542323731507864 19.600485785335948 13.313706232058237 27.54348425109795 2 13.625540222434829 20.857146461167055 39.993731770221835 25.52358154617628 3 16.705631563652666 25.082607360460145 30.347453034725895 27.86430804116129 4 11.063843618500396 17.52354561499002 38.19920070556799 33.21341006094159 5 12.415154155935264 38.38355346362328 36.35409864717577 12.847193733265689 6 31.252457688818637 38.04078887431039 18.204174080289043 12.502579356581933 7 27.28151914778189 31.796347705997757 22.943613289486006 17.978519856734344 8 24.893490564435723 36.939379016391854 20.86900831358241 17.298122105590018 9 26.149104512295523 14.752733618302036 20.10048130311326 38.99768056628918 10 13.988231681315211 29.406339794799102 35.351965291755626 21.253463232130063 11 33.913818814404735 22.48321268588294 24.873652479069825 18.729316020642504 12 22.41146456399554 25.199550979975534 32.20845473270793 20.180529723321 13 28.503214839781933 21.881382259828133 26.04451973388675 23.570883166503183 14 20.666322554605152 22.007098281509542 28.135381576291973 29.191197587593336 15 23.009317068485256 30.54393711391118 23.496030325345473 22.95071549225809 16 22.035017352470046 28.286369603245987 27.611626785577414 22.066986258706557 17 21.702530899330267 28.606571575381846 27.825773047312257 21.86512447797563 18 21.50580263472031 27.879578103133362 29.252111328288017 21.36250793385831 19 23.01741844533123 27.315562696409877 27.801742329645506 21.865276528613382 20 23.27511014476724 27.410305145393032 28.061034899955274 21.253549809884458 21 22.8739278922653 26.86648620328327 27.399289789265342 22.86029611518609 22 22.78053376049179 27.95703254237222 27.29834566653116 21.96408803060483 23 22.052676518246123 27.68728835760651 27.693837749725898 22.56619737442147 24 22.543560666699655 27.373339581580865 28.144522741583543 21.938577010135937 25 22.467080241547393 27.29293442826539 27.735454376106055 22.504530954081154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 153.0 1 153.0 2 262.5 3 372.0 4 372.0 5 372.0 6 886.0 7 1400.0 8 1400.0 9 1400.0 10 2125.5 11 2851.0 12 2851.0 13 2851.0 14 5486.0 15 8121.0 16 8121.0 17 8121.0 18 15787.5 19 23454.0 20 23454.0 21 23454.0 22 37891.0 23 52328.0 24 52328.0 25 52328.0 26 75271.0 27 98214.0 28 98214.0 29 98214.0 30 126654.5 31 155095.0 32 155095.0 33 155095.0 34 178663.0 35 202231.0 36 202231.0 37 202231.0 38 220376.5 39 238522.0 40 238522.0 41 238522.0 42 250736.5 43 262951.0 44 262951.0 45 262951.0 46 271128.5 47 279306.0 48 279306.0 49 279306.0 50 265878.5 51 252451.0 52 252451.0 53 252451.0 54 222403.0 55 192355.0 56 192355.0 57 192355.0 58 163349.5 59 134344.0 60 134344.0 61 134344.0 62 108084.5 63 81825.0 64 81825.0 65 81825.0 66 62864.5 67 43904.0 68 43904.0 69 43904.0 70 31770.0 71 19636.0 72 19636.0 73 19636.0 74 13617.0 75 7598.0 76 7598.0 77 7598.0 78 5199.5 79 2801.0 80 2801.0 81 2801.0 82 1908.0 83 1015.0 84 1015.0 85 1015.0 86 687.0 87 359.0 88 359.0 89 359.0 90 218.5 91 78.0 92 78.0 93 78.0 94 45.5 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17847284680441916 2 0.009217135333742769 3 0.005869859870436184 4 0.0029106743159187693 5 0.07824862785961624 6 0.32027119722826186 7 0.028670142011799876 8 0.014213792909403321 9 0.004802612621265969 10 0.003929410326490339 11 0.0 12 0.0 13 0.0 14 0.001649382112353969 15 0.0 16 0.0 17 0.019938119064043566 18 0.028621630773201227 19 0.0 20 0.0 21 8.732022947756307E-4 22 1.9404495439458462E-4 23 0.005724326154640246 24 0.0 25 9.702247719729231E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2061378.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.70398184383086 #Duplication Level Percentage of deduplicated Percentage of total 1 82.71536505090234 60.13736398866646 2 11.875009102050301 17.26720892301583 3 3.001816529022824 6.547320432737604 4 1.0710587495405839 3.114809435210992 5 0.4581755856049855 1.6655594728555723 6 0.23942850289883055 1.044444331659131 7 0.1430227791925567 0.7278827879168911 8 0.09796264828127474 0.5697819681612311 9 0.068735816622412 0.4497630807363057 >10 0.29778660158966774 3.729943276642114 >50 0.017471318712129945 0.8581185997431037 >100 0.011896460298379588 1.8229826404752543 >500 0.0014025870871125971 0.7537796281504328 >1k 8.682681967838879E-4 1.3110414340289824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3155 0.1530529577787286 No Hit CCCATGTACTCTGCGTTGATACCAC 3106 0.15067590708739495 No Hit GTATCAACGCAGAGTACTTTTTTTT 3018 0.14640691809071407 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2236 0.10847112950657278 No Hit GTCCTACAGTGGACATTTCTAAATT 2230 0.10818006207498092 No Hit TATCAACGCAGAGTACTTTTTTTTT 2167 0.10512385404326621 No Hit CTTTAGGACGTGAAATATGGCGAGG 2163 0.10492980908887162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.851123859864615E-5 2 0.0 0.0 0.0 0.0 4.851123859864615E-5 3 0.0 0.0 0.0 0.0 4.851123859864615E-5 4 0.0 0.0 0.0 0.0 4.851123859864615E-5 5 0.0 0.0 0.0 0.0 4.851123859864615E-5 6 0.0 0.0 0.0 0.0 4.851123859864615E-5 7 0.0 0.0 0.0 0.0 4.851123859864615E-5 8 4.851123859864615E-5 0.0 0.0 0.0 4.851123859864615E-5 9 4.851123859864615E-5 0.0 0.0 4.851123859864615E-5 1.4553371579593844E-4 10 4.851123859864615E-5 0.0 0.0 4.851123859864615E-5 1.4553371579593844E-4 11 4.851123859864615E-5 0.0 0.0 9.70224771972923E-5 1.4553371579593844E-4 12 4.851123859864615E-5 0.0 0.0 1.4553371579593844E-4 1.4553371579593844E-4 13 4.851123859864615E-5 0.0 0.0 1.4553371579593844E-4 1.4553371579593844E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 510 0.0 12.694876 4 GGCGAGG 630 0.0 12.648992 19 GTCGAGG 70 1.10554996E-4 12.197242 19 CGTACTA 65 8.1199274E-4 11.675991 19 ACACCGT 90 7.2930216E-6 11.635269 6 TGGCGAG 1265 0.0 11.028936 18 ACCGTAC 70 0.0015028365 10.847518 17 CGTGCGC 70 0.0015092377 10.841992 10 GCGCCAC 80 3.802833E-4 10.678025 13 TATGTCG 90 9.6035554E-5 10.546199 16 GACGTGA 765 0.0 10.544151 7 GTAGGAC 1190 0.0 10.40177 3 TGTAGGA 1260 0.0 10.202232 2 TATGGCG 1435 0.0 10.186085 16 AGGACGT 1355 0.0 10.118424 5 CTACACT 330 0.0 10.098196 4 TGTCGAG 95 1.6604118E-4 9.990408 18 ATGGCGA 1445 0.0 9.9842205 17 TTAATAC 210 5.456968E-12 9.975031 3 AAGGCGT 115 1.001606E-5 9.933668 6 >>END_MODULE