FastQCFastQC Report
Thu 26 May 2016
SRR1513151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773297
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33750.12169630587708422No Hit
GTACATGGGAAGCAGTGGTATCAAC30890.11138367077164833No Hit
GTCCTAAAGTGTGTATTTCTCATTT29210.10532589910132235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7600.012.9164514
CGTGCGC1052.7671922E-711.74719810
GGCGAGG7300.011.56917119
TGGCGAG17400.011.50716418
AGACCGT1251.8715582E-811.3843086
GTCCTAC18050.011.2202471
TAGGACT5150.011.1035954
TGTAGGA20500.010.98035052
ATAGGAC3950.010.8578263
GTATAAA4400.010.8557811
GTAGGAC19700.010.6918663
GGCGAGA11300.010.664988519
GGACGTG16550.010.6047186
GTCCTAA9600.010.5482011
GTCCTAT3000.010.5083961
AGGACGT16800.010.4731665
TCCTACA20550.010.4430842
AGGACCT14350.010.2729545
TATGGCG19400.010.17411616
ATGGCGA20100.010.15026817
Produced by FastQC (version 0.11.5)