##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513151_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2773297
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.40296946197973	31.0	30.0	34.0	25.0	34.0
2	29.273779908895442	31.0	30.0	34.0	22.0	34.0
3	29.44303765518082	31.0	30.0	34.0	23.0	34.0
4	32.49683066761332	37.0	33.0	37.0	22.0	37.0
5	32.64859876169051	37.0	33.0	37.0	24.0	37.0
6	33.001599540186284	37.0	35.0	37.0	26.0	37.0
7	32.97047449299516	37.0	35.0	37.0	26.0	37.0
8	32.84730881690638	37.0	35.0	37.0	25.0	37.0
9	34.08455567506834	39.0	35.0	39.0	23.0	39.0
10	33.653602553206525	39.0	34.0	39.0	18.0	39.0
11	34.12545933594563	39.0	35.0	39.0	25.0	39.0
12	34.11735814808151	39.0	35.0	39.0	25.0	39.0
13	34.16414974667337	39.0	35.0	39.0	25.0	39.0
14	34.37531609488634	39.0	34.0	41.0	17.0	41.0
15	34.81925808883794	40.0	34.0	41.0	19.0	41.0
16	34.66680200497819	39.0	34.0	41.0	17.0	41.0
17	34.2700046190509	39.0	33.0	41.0	17.0	41.0
18	34.00115314010724	39.0	33.0	41.0	16.0	41.0
19	34.63473043096358	39.0	34.0	41.0	18.0	41.0
20	34.85397344748868	40.0	35.0	41.0	22.0	41.0
21	34.89685093230188	40.0	35.0	41.0	22.0	41.0
22	34.57319464882413	40.0	34.0	41.0	18.0	41.0
23	34.11405413844965	39.0	34.0	41.0	10.0	41.0
24	34.36692824461282	39.0	34.0	41.0	15.0	41.0
25	34.01541017784969	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7548325357579451
1101	2	1.0751635145169374
1101	3	0.9291259099768396
1101	4	1.3134759301016317
1101	5	1.0859209733395971
1101	6	0.8476106562913728
1101	7	0.7710561806025069
1101	8	0.8626769547253801
1101	9	1.2079568417633055
1101	10	1.5503099893774035
1101	11	1.0721866604778967
1101	12	0.947869989975672
1101	13	0.8653229727940968
1101	14	1.8819733296695063
1101	15	1.4956371226229237
1101	16	1.5847900749511936
1101	17	1.8958962628374394
1101	18	1.91724727876462
1101	19	1.5168226488359693
1101	20	1.158085100378706
1101	21	1.165905951544552
1101	22	1.3859055033462937
1101	23	1.6852189648932523
1101	24	1.4109930082575204
1101	25	1.7371838972845595
1102	1	1.0309744226426538
1102	2	1.2348900086762882
1102	3	1.0994320378828384
1102	4	1.37483536108725
1102	5	1.3909429378449545
1102	6	1.0954390351962857
1102	7	1.0885102843360883
1102	8	1.1059287710944758
1102	9	1.394014766938831
1102	10	1.584241826832418
1102	11	1.3045578338790733
1102	12	1.2790589763996962
1102	13	1.2051376305680037
1102	14	2.1972314412568323
1102	15	1.7647207252601902
1102	16	1.8477116979562425
1102	17	2.1934580881451353
1102	18	2.4653896545983187
1102	19	1.8359465133665438
1102	20	1.5776583395893624
1102	21	1.4100335977899618
1102	22	1.7078567154050006
1102	23	1.9916148760573336
1102	24	1.7208876550300758
1102	25	2.0337831042704195
1103	1	0.5823930788497123
1103	2	0.8197525732609385
1103	3	0.5778599993220439
1103	4	1.0860241508667983
1103	5	0.9066307272265135
1103	6	0.6065347186274934
1103	7	0.5963973104977143
1103	8	0.623272124615859
1103	9	0.9695585926552752
1103	10	1.2246944641552773
1103	11	0.722461086118038
1103	12	0.6443602033504732
1103	13	0.6657518443236086
1103	14	1.4750447567252678
1103	15	1.1250430136953753
1103	16	1.1742707790668163
1103	17	1.5361658080481035
1103	18	1.8802445293549752
1103	19	1.174176304156397
1103	20	0.8739358865522391
1103	21	0.9735112369587355
1103	22	1.1681188538975391
1103	23	1.531207585307996
1103	24	1.188711937958118
1103	25	1.4565185219613426
1104	1	0.7814081780815307
1104	2	0.9063038725946626
1104	3	0.8066403434460732
1104	4	1.2413454314742935
1104	5	1.0821506076309078
1104	6	0.8272347628451868
1104	7	0.8311180187834779
1104	8	0.8342808255401337
1104	9	1.1681003403936856
1104	10	1.3977519134751404
1104	11	0.9069022626097052
1104	12	0.9227431973828786
1104	13	0.873632698778593
1104	14	1.8324014845872227
1104	15	1.3739674917366713
1104	16	1.3761163123988638
1104	17	1.8515125012883829
1104	18	1.9574585136128988
1104	19	1.285095709704322
1104	20	1.1770001656067421
1104	21	1.16613913681892
1104	22	1.4460109383569204
1104	23	1.7884768094343784
1104	24	1.5027149006153024
1104	25	1.6878032215641028
1105	1	0.6635427918677088
1105	2	0.8821749662863887
1105	3	0.7831358923379312
1105	4	1.1590942385091125
1105	5	1.0951835346776306
1105	6	0.7462683562616235
1105	7	0.7117319882512447
1105	8	0.7893232480000592
1105	9	1.0671094528929501
1105	10	1.323064514043331
1105	11	0.9034286861579872
1105	12	0.8264069520565229
1105	13	0.6514307233973327
1105	14	1.4925873861080063
1105	15	1.2030990727591941
1105	16	1.3866020447697238
1105	17	1.805028951313858
1105	18	1.3010260922399084
1105	19	1.2207050721277426
1105	20	1.0626243401927624
1105	21	1.0116745663041442
1105	22	1.1481863015745901
1105	23	1.4437363348626349
1105	24	1.2443364381390936
1105	25	1.4458538272998496
1106	1	0.7126793266482139
1106	2	0.9133021969321717
1106	3	0.81535881226106
1106	4	1.3125160821567547
1106	5	1.092574742473765
1106	6	0.7985117865225106
1106	7	0.7984912686790722
1106	8	0.7840753187752085
1106	9	1.1020165098215529
1106	10	0.9648244651605893
1106	11	0.9388316049845713
1106	12	0.9411944986815115
1106	13	0.8312992820633482
1106	14	1.8109377935733804
1106	15	1.4510170907185014
1106	16	1.552424056555239
1106	17	1.811483949087993
1106	18	1.886340717993484
1106	19	1.4788794658607145
1106	20	1.1262010424076934
1106	21	1.0624948936074787
1106	22	1.2437733375790643
1106	23	1.6619110872161684
1106	24	1.294224179557112
1106	25	1.4879343300307113
1107	1	0.5436326133059026
1107	2	0.8270835461236032
1107	3	0.7796346568870405
1107	4	1.0000857772234752
1107	5	0.9481727721437707
1107	6	0.708701703174107
1107	7	0.6878686070751456
1107	8	0.803579066585371
1107	9	1.0039511372885812
1107	10	1.243300740902292
1107	11	0.8449468830757851
1107	12	0.8627539985000752
1107	13	0.8453342231227197
1107	14	1.6824636442527776
1107	15	1.0365703711626608
1107	16	1.2051872438632714
1107	17	1.612851330552374
1107	18	1.7209866177564095
1107	19	1.2211064978583224
1107	20	1.0404645301956066
1107	21	0.8486754710850235
1107	22	1.179150136307996
1107	23	1.472437416607164
1107	24	1.2145827149411872
1107	25	1.5052724429519344
1108	1	0.5703242880776962
1108	2	0.8128642739194092
1108	3	0.5746292828393855
1108	4	1.027472350974449
1108	5	0.883848275223869
1108	6	0.5491425343453997
1108	7	0.52557081958129
1108	8	0.4818565444040175
1108	9	0.7421458731329622
1108	10	1.061027732203165
1108	11	0.6993954642011175
1108	12	0.5535007820899764
1108	13	0.5372943978793572
1108	14	1.5249666652882112
1108	15	1.09661371303946
1108	16	1.2069362148973894
1108	17	1.4880255529965822
1108	18	1.7203920881586683
1108	19	1.100381265758969
1108	20	0.8937543564896657
1108	21	0.8275061583902144
1108	22	1.1165688039290629
1108	23	1.4384424453620284
1108	24	1.1185360187915379
1108	25	1.4087088030129067
1109	1	0.3459964216021376
1109	2	0.45781199419866425
1109	3	0.463400257396966
1109	4	0.7154679732957234
1109	5	0.7854354457952439
1109	6	0.44159636651749423
1109	7	0.3558355416222767
1109	8	0.4559485822169549
1109	9	0.571275560555172
1109	10	0.9029747074385384
1109	11	0.5273833478484988
1109	12	0.47796660833316196
1109	13	0.422003442083259
1109	14	1.3994206564262939
1109	15	0.948891293856633
1109	16	1.137976856910349
1109	17	1.364164845632878
1109	18	1.5470995095119946
1109	19	0.8454277720446726
1109	20	0.7265339291855923
1109	21	0.5732538182274567
1109	22	0.8819985492603166
1109	23	1.2281889644872948
1109	24	0.9698161317097984
1109	25	1.2346572054484
1110	1	0.2609126037250178
1110	2	0.5197067459379312
1110	3	0.3552939218251545
1110	4	0.8580929838668041
1110	5	0.660441702148681
1110	6	0.3502676292308351
1110	7	0.3093165335261219
1110	8	0.3396585048798002
1110	9	0.5592543010347271
1110	10	0.8799638395054146
1110	11	0.5254468985922713
1110	12	0.42080101258161307
1110	13	0.2502352585313403
1110	14	0.9900881618757964
1110	15	0.7373303293599278
1110	16	0.9517970553532535
1110	17	1.2165317320054214
1110	18	0.96657851491576
1110	19	0.6871569159511566
1110	20	0.4398680305402465
1110	21	0.30268853727835676
1110	22	0.6153342125459602
1110	23	-0.6814093172160227
1110	24	0.16156883402300792
1110	25	0.6227474384608911
1111	1	0.30578705489995883
1111	2	0.6422952066119407
1111	3	0.5657568247853924
1111	4	0.9595471559954376
1111	5	0.8288147546212912
1111	6	0.46561184248881915
1111	7	0.4634778581473924
1111	8	0.5372703407501547
1111	9	0.7364850608883913
1111	10	1.0162881115728055
1111	11	0.7075277206880841
1111	12	0.5474169592696612
1111	13	0.39230105658742076
1111	14	1.2499106119688506
1111	15	0.9426837043404106
1111	16	1.0524997609502194
1111	17	1.3359087221774644
1111	18	1.5798759434960132
1111	19	1.1679880380410026
1111	20	0.8112068335354863
1111	21	0.593771635307732
1111	22	0.9670151526500064
1111	23	1.4240716531096496
1111	24	0.9830151904341236
1111	25	1.166581169599361
1112	1	0.3041716849842899
1112	2	0.45087639086419884
1112	3	0.44249693082835506
1112	4	0.8548122662883415
1112	5	0.6427226497745551
1112	6	0.5114586838181623
1112	7	0.32614680005490015
1112	8	0.4654657361638783
1112	9	0.6859422156655341
1112	10	0.9126600163546783
1112	11	0.42778499372344214
1112	12	0.5148177992641152
1112	13	0.39169900615195985
1112	14	1.0325875147350558
1112	15	0.8838493476717915
1112	16	0.7300365931740203
1112	17	1.2911462119436337
1112	18	1.3826792070491933
1112	19	0.8428403897772299
1112	20	0.6656317625278092
1112	21	0.5688286086142398
1112	22	0.8330136103386039
1112	23	1.1452051559436285
1112	24	0.8192553726059444
1112	25	1.1227695746989497
1113	1	0.12667511093205164
1113	2	0.3485073660667837
1113	3	0.264257024743884
1113	4	0.5721771879323398
1113	5	0.5480904889630835
1113	6	0.23593487399316615
1113	7	0.2247011079045791
1113	8	0.26037079594901513
1113	9	0.4204929448624313
1113	10	0.6689111883416885
1113	11	0.33249639057746805
1113	12	0.24784826981174746
1113	13	0.13692016350303504
1113	14	0.7791888496117352
1113	15	0.48721503774007147
1113	16	0.6402043105096027
1113	17	0.8669049298291114
1113	18	0.8242745807015979
1113	19	0.5734074129698783
1113	20	0.31594432955172636
1113	21	0.31479781575559684
1113	22	0.45293640189514406
1113	23	0.617212597766283
1113	24	0.5090353555525411
1113	25	0.834422120839271
1114	1	-1.0199950677524292
1114	2	-0.7099115883522487
1114	3	-0.7495102509116833
1114	4	-0.4588611239925058
1114	5	-3.539516318273634
1114	6	-1.2756723482694383
1114	7	-0.9740439565564074
1114	8	-0.6916776735516592
1114	9	-0.40343074925780087
1114	10	-0.3288356109437487
1114	11	-0.740292124494836
1114	12	-0.7482808625283113
1114	13	-0.7842709587602599
1114	14	-6.152632031794418
1114	15	-2.585043375163579
1114	16	-1.4764104000870049
1114	17	-0.4668518219557569
1114	18	-6.364588085933125
1114	19	-2.4590817636666813
1114	20	-1.463152507926651
1114	21	-1.1127728066654683
1114	22	-0.9120952103261075
1114	23	-0.4094778916028403
1114	24	-0.5604608204834065
1114	25	-0.4339378507667817
1115	1	-0.9617470465470852
1115	2	-0.633343083466432
1115	3	-0.5857859921363264
1115	4	-3.220358045276928
1115	5	-1.3361785342579005
1115	6	-0.9331392823060725
1115	7	-0.8251932595506943
1115	8	-0.6368350033396268
1115	9	-0.5196117353655225
1115	10	-0.7540208672768642
1115	11	-0.855416801181299
1115	12	-0.9359141409779141
1115	13	-0.9923002296215557
1115	14	-6.32296929342127
1115	15	-2.6636106534225945
1115	16	-1.507224178321529
1115	17	-0.7007704257196181
1115	18	-0.3342862089397656
1115	19	-0.7797732987269086
1115	20	-0.6729261438405842
1115	21	-0.8988713115171123
1115	22	-2.16721830390091
1115	23	-1.1933200352499114
1115	24	-0.8749231243930069
1115	25	-0.5468732681634734
1116	1	-0.28297684290387437
1116	2	-0.06654841295487657
1116	3	-0.20395299084731988
1116	4	0.05901388581853695
1116	5	-0.5909855702940021
1116	6	-0.24958929771800342
1116	7	-0.22228848729873363
1116	8	-0.21225893973103638
1116	9	-0.24576157332161586
1116	10	0.011905912847048228
1116	11	-0.29424684026455594
1116	12	-0.6023159050923184
1116	13	-0.4910872635438821
1116	14	0.4294822281207473
1116	15	-0.09701470146784175
1116	16	-0.026825981646631192
1116	17	0.361483242599121
1116	18	0.13903386760602388
1116	19	-0.01750607157286055
1116	20	-0.4114492935113887
1116	21	-0.4968847006171302
1116	22	-0.4477029256581275
1116	23	-1.1503272838132617
1116	24	-0.7049434268333243
1116	25	-0.07014382747625802
1201	1	0.7194538131099684
1201	2	0.8760043986065718
1201	3	0.8977713088927182
1201	4	1.2367452722435885
1201	5	1.1156355409624084
1201	6	0.7869807133165665
1201	7	0.7384592209246179
1201	8	0.8175655855385955
1201	9	1.0094087319292981
1201	10	1.483635437443418
1201	11	0.8312337483935224
1201	12	0.8936779183463983
1201	13	0.77185387395145
1201	14	1.715281189095208
1201	15	1.4115221774878393
1201	16	1.4967629136780687
1201	17	1.7134041302301242
1201	18	1.1410124734806786
1201	19	1.0960956925593663
1201	20	1.1176891414760703
1201	21	0.9628101669962064
1201	22	1.3224633390242673
1201	23	1.5132189771501459
1201	24	1.0855362398484303
1201	25	1.5024256185199292
1202	1	0.9289981390044524
1202	2	1.0993597200725276
1202	3	0.9783800240520897
1202	4	1.394600353284396
1202	5	1.2372104433632458
1202	6	0.973992447312682
1202	7	0.8890237276399446
1202	8	1.010284954870528
1202	9	1.2662882296929325
1202	10	1.527454781844149
1202	11	1.074492356982823
1202	12	0.9260315532335497
1202	13	0.9742553392718634
1202	14	1.9387011203158409
1202	15	1.5225386690676714
1202	16	1.5537861861597406
1202	17	1.959789106372554
1202	18	2.2028880826497854
1202	19	1.6808671733813227
1202	20	1.3915193326296702
1202	21	1.355879461798743
1202	22	1.5035888601655927
1202	23	1.7393782178582313
1202	24	1.5076392129059286
1202	25	1.951644032636274
1203	1	0.6882395952943803
1203	2	0.8445636054122581
1203	3	0.7536812803752646
1203	4	1.1074207869415034
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2211	14	-0.2143075271548298
2211	15	-0.467990843613741
2211	16	-6.95683478569541
2211	17	-2.325418038802944
2211	18	-1.133415645267867
2211	19	-0.9398061933363522
2211	20	-1.0602936223478423
2211	21	-1.028834452978458
2211	22	-0.9216267470120414
2211	23	-0.47555663631712974
2211	24	-0.8591045391226402
2211	25	-7.88520740706327
2212	1	-1.4425713693682276
2212	2	-3.8201000982265754
2212	3	-1.6790434645562051
2212	4	-3.6287444274036353
2212	5	-1.2616886701587333
2212	6	-1.129801612183261
2212	7	-1.0285771702009328
2212	8	-0.9140744419538116
2212	9	-1.108996278880781
2212	10	-0.5045537437983327
2212	11	-1.0481915858951965
2212	12	-1.0550582413433958
2212	13	-1.2755187145950941
2212	14	-6.763942624254021
2212	15	-5.4519643805495726
2212	16	-3.006706629889514
2212	17	-7.450918249244808
2212	18	-3.103142843563287
2212	19	-2.0147370598964827
2212	20	-2.1110429926663272
2212	21	-1.6206273847624146
2212	22	-1.536870793809051
2212	23	-0.952790279394577
2212	24	-1.269841430896335
2212	25	-1.0625285150396735
2213	1	-0.21525877296394924
2213	2	-0.13343257287699117
2213	3	-0.14938532044919484
2213	4	0.2974995828879159
2213	5	0.1107147359150531
2213	6	-0.23109090087772444
2213	7	-0.30230961197906936
2213	8	-0.337499812399102
2213	9	-0.011987498189455437
2213	10	-3.931780761650508
2213	11	-1.408471363754451
2213	12	-0.8589577153869357
2213	13	-0.5568663041720541
2213	14	0.22861765212598328
2213	15	-0.34170830866042934
2213	16	-0.1591802688507542
2213	17	0.25106570339382017
2213	18	0.31922326511752175
2213	19	-0.20164243514618363
2213	20	-0.6028284789231861
2213	21	-0.5391906607378374
2213	22	-0.7614275711878662
2213	23	-1.0862754362964964
2213	24	-0.534607879678795
2213	25	-0.24332486179164192
2214	1	-1.016175623711991
2214	2	-0.8246524713888235
2214	3	-0.72571410081121
2214	4	-0.3333303103296714
2214	5	-3.4529414776680234
2214	6	-1.4574708646091317
2214	7	-1.186448881328154
2214	8	-0.8855923547454267
2214	9	-0.7407330406818247
2214	10	-4.381643546862534
2214	11	-1.9248124364924593
2214	12	-1.294973654399712
2214	13	-1.0292690738360477
2214	14	-0.2674075784321346
2214	15	-0.5998791540503277
2214	16	-0.611705150262793
2214	17	-0.3931745558870716
2214	18	0.01964547324205057
2214	19	-0.8655922376010068
2214	20	-1.0885697316349692
2214	21	-1.0334413982043316
2214	22	-0.7368103482455481
2214	23	-0.6446275200672105
2214	24	-1.0304418025289195
2214	25	-0.7164768882841415
2215	1	-2.3223604263950186
2215	2	-4.786401366210782
2215	3	-3.043457733170282
2215	4	-2.0497073819052893
2215	5	-2.1470762136783392
2215	6	-2.1855948939234437
2215	7	-2.4127425156151574
2215	8	-2.5770149454906885
2215	9	-8.323616442403114
2215	10	-4.064695422226709
2215	11	-3.3233723260069006
2215	12	-3.5818479096117812
2215	13	-3.0782091279472255
2215	14	-8.373616824498747
2215	15	-5.089966616447015
2215	16	-9.49869539262324
2215	17	-4.806459251278099
2215	18	-3.2619887316140854
2215	19	-3.761271361839949
2215	20	-3.5098138952854327
2215	21	-3.6720441880524746
2215	22	-9.653408965428813
2215	23	-10.90647822725354
2215	24	-7.046609289471448
2215	25	-5.243465626717846
2216	1	-1.7417768359239467
2216	2	-1.766751484169955
2216	3	-1.6566303699999878
2216	4	-6.784940275511929
2216	5	-3.3932247160913427
2216	6	-2.5352553830163593
2216	7	-2.0302994977330044
2216	8	-7.738306731345517
2216	9	-3.777038213669872
2216	10	-2.0195948261406222
2216	11	-1.9822310178249367
2216	12	-2.1426215891820064
2216	13	-1.9408465397733963
2216	14	-1.2392625732888192
2216	15	-1.843078194727795
2216	16	-1.5795901955360918
2216	17	-1.2477384901164186
2216	18	-7.2167681995515345
2216	19	-4.378812837949408
2216	20	-3.6743806614803987
2216	21	-2.9783494921910503
2216	22	-2.5963235098890607
2216	23	-1.8097920111654915
2216	24	-2.1283122895285445
2216	25	-1.6006455446893497
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132240.0
3	2.0
4	1150.0
5	6413.0
6	7627.0
7	6800.0
8	6287.0
9	5902.0
10	5703.0
11	5415.0
12	5280.0
13	5237.0
14	5257.0
15	5448.0
16	5341.0
17	5780.0
18	6717.0
19	8015.0
20	10411.0
21	13003.0
22	16437.0
23	19353.0
24	22429.0
25	25705.0
26	28633.0
27	32934.0
28	36506.0
29	43537.0
30	52376.0
31	61978.0
32	79356.0
33	104521.0
34	137577.0
35	183604.0
36	261906.0
37	380016.0
38	1038401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.438446437827	18.396159798887886	12.491344919192155	25.67404884409296
2	14.357133172438106	19.947103433938047	41.63910657953015	24.0566568140937
3	18.378498771206495	24.521453899362953	29.722882387630566	27.377164941799986
4	11.33496979194029	16.736804920245287	39.1411309680166	32.78709431979783
5	12.691306193313167	37.91328103363373	36.49744346592409	12.89796930712901
6	32.01870490935346	37.14823705580168	18.19844428703943	12.634613747805426
7	28.206768706992314	31.39875070327039	22.359598378510942	18.034882211226357
8	25.118417436701577	36.58390128742673	20.74752111389929	17.550160161972407
9	26.469284586735025	14.92735940818556	20.08499831034842	38.518357694730994
10	13.833520815578423	28.682475284983234	35.820265177676546	21.663738721761803
11	34.480827234304066	22.22443826597021	24.60698183617538	18.687752663550338
12	23.12608971339411	24.61993033047937	31.781362645797067	20.472617310329454
13	28.94436431274231	21.63765153638262	25.7232502284024	23.69473392247267
14	20.405335268482048	21.487292110674254	28.737319071229606	29.370053549614095
15	23.288655584045294	30.22320597601571	23.32252823442327	23.165610205515723
16	22.34720588245902	27.680997490548986	27.692033593030374	22.279763033961622
17	21.73788042264714	28.094289372100395	28.148002252405806	22.019827952846658
18	21.651827580933254	27.232788247617236	29.55037642367213	21.565007747777383
19	23.503924384056912	27.044147652493006	27.35837249421739	22.093555469232694
20	23.75859255577542	27.134333504781676	27.536215156370268	21.570858783072637
21	23.352125102757157	26.62891960611951	26.84487646236193	23.1740788287614
22	23.212531605522674	27.601681491365817	26.940910073111613	22.244876829999896
23	22.401580682091158	27.28523333311692	27.646246637479738	22.666939347312184
24	23.16234151585608	26.9054984460482	27.624735521552402	22.30742451654332
25	22.961843405741163	26.8779263128278	27.514590983529686	22.64563929790135
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	191.0
1	191.0
2	322.0
3	453.0
4	453.0
5	453.0
6	875.0
7	1297.0
8	1297.0
9	1297.0
10	2244.0
11	3191.0
12	3191.0
13	3191.0
14	6572.0
15	9953.0
16	9953.0
17	9953.0
18	19293.5
19	28634.0
20	28634.0
21	28634.0
22	46785.5
23	64937.0
24	64937.0
25	64937.0
26	94211.0
27	123485.0
28	123485.0
29	123485.0
30	159736.0
31	195987.0
32	195987.0
33	195987.0
34	229300.5
35	262614.0
36	262614.0
37	262614.0
38	288958.5
39	315303.0
40	315303.0
41	315303.0
42	333776.5
43	352250.0
44	352250.0
45	352250.0
46	363588.5
47	374927.0
48	374927.0
49	374927.0
50	360661.5
51	346396.0
52	346396.0
53	346396.0
54	309052.5
55	271709.0
56	271709.0
57	271709.0
58	231809.5
59	191910.0
60	191910.0
61	191910.0
62	155398.5
63	118887.0
64	118887.0
65	118887.0
66	91399.0
67	63911.0
68	63911.0
69	63911.0
70	46625.5
71	29340.0
72	29340.0
73	29340.0
74	20418.0
75	11496.0
76	11496.0
77	11496.0
78	7871.5
79	4247.0
80	4247.0
81	4247.0
82	2881.0
83	1515.0
84	1515.0
85	1515.0
86	998.0
87	481.0
88	481.0
89	481.0
90	307.0
91	133.0
92	133.0
93	133.0
94	81.5
95	30.0
96	30.0
97	30.0
98	25.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16918490879267528
2	0.2712295149059044
3	0.2731766557999378
4	0.47856396195575157
5	0.26838091989426305
6	0.019795932422672365
7	0.019363234446220512
8	0.017271860893369878
9	0.019327176281516187
10	0.021274317175549534
11	0.02120220084614089
12	0.02808931030466625
13	0.028053252139961928
14	0.026250343904745867
15	0.04143083124526511
16	0.020877677363802003
17	0.04146688940996943
18	0.03205570842214159
19	0.02740420517528415
20	0.030252800186925527
21	0.035481234069052105
22	0.044495775245132416
23	0.029675869551656388
24	0.0480294753861559
25	0.037392316798381134
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2773297.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.08188830683423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92056162377959	57.51986323067797
2	12.621647996655325	17.943411462928637
3	3.5195501489929906	7.505288117430645
4	1.316777768635209	3.7439620110020133
5	0.5841937047775454	2.076279583627658
6	0.3119294161195037	1.3303519149733547
7	0.1781098689801397	0.8862270069233819
8	0.11720728178878859	0.6665051930286653
9	0.0847433582164741	0.542134613114051
>10	0.3167911729044456	3.7832624812124784
>50	0.016576360598021596	0.8037473213547431
>100	0.0096084934945722	1.3638093391777175
>500	0.001432856480325766	0.6727565780757939
>1k	8.699485770886858E-4	1.1624011464728854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3375	0.12169630587708422	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3089	0.11138367077164833	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2921	0.10532589910132235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.211632940864249E-5
2	0.0	0.0	0.0	0.0	7.211632940864249E-5
3	0.0	0.0	0.0	0.0	7.211632940864249E-5
4	0.0	3.6058164704321246E-5	0.0	7.211632940864249E-5	7.211632940864249E-5
5	0.0	7.211632940864249E-5	0.0	7.211632940864249E-5	1.4423265881728498E-4
6	0.0	7.211632940864249E-5	0.0	7.211632940864249E-5	1.4423265881728498E-4
7	0.0	1.0817449411296375E-4	0.0	7.211632940864249E-5	1.4423265881728498E-4
8	0.0	1.0817449411296375E-4	0.0	7.211632940864249E-5	1.4423265881728498E-4
9	0.0	1.0817449411296375E-4	0.0	7.211632940864249E-5	1.4423265881728498E-4
10	0.0	1.0817449411296375E-4	0.0	7.211632940864249E-5	1.4423265881728498E-4
11	0.0	1.0817449411296375E-4	0.0	1.4423265881728498E-4	1.4423265881728498E-4
12	0.0	1.0817449411296375E-4	0.0	1.8029082352160624E-4	2.524071529302487E-4
13	0.0	1.0817449411296375E-4	0.0	1.8029082352160624E-4	2.524071529302487E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	760	0.0	12.916451	4
CGTGCGC	105	2.7671922E-7	11.747198	10
GGCGAGG	730	0.0	11.569171	19
TGGCGAG	1740	0.0	11.507164	18
AGACCGT	125	1.8715582E-8	11.384308	6
GTCCTAC	1805	0.0	11.220247	1
TAGGACT	515	0.0	11.103595	4
TGTAGGA	2050	0.0	10.9803505	2
ATAGGAC	395	0.0	10.857826	3
GTATAAA	440	0.0	10.855781	1
GTAGGAC	1970	0.0	10.691866	3
GGCGAGA	1130	0.0	10.6649885	19
GGACGTG	1655	0.0	10.604718	6
GTCCTAA	960	0.0	10.548201	1
GTCCTAT	300	0.0	10.508396	1
AGGACGT	1680	0.0	10.473166	5
TCCTACA	2055	0.0	10.443084	2
AGGACCT	1435	0.0	10.272954	5
TATGGCG	1940	0.0	10.174116	16
ATGGCGA	2010	0.0	10.150268	17
>>END_MODULE