FastQCFastQC Report
Thu 26 May 2016
SRR1513151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773297
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC35580.12829495001797497No Hit
GTCCTACAGTGGACATTTCTAAATT33250.11989339764186815No Hit
CCCATGTACTCTGCGTTGATACCAC33030.11910011801837307No Hit
CTTTAGGACGTGAAATATGGCGAGG30440.10976105335995388No Hit
GTCCTAAAGTGTGTATTTCTCATTT29860.10766967980710325No Hit
GTATCAACGCAGAGTACTTTTTTTT29480.10629946954833903No Hit
CTGTAGGACGTGGAATATGGCAAGA29230.10539801543073102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6550.013.0797424
GGCGAGG7050.011.306581519
TGGCGAG17050.010.85538818
GTCCTAA11200.010.7156291
CTAGGAC3750.010.4079763
GTAGGAC18250.010.2236723
GTCCTAC19000.010.1265821
AGGACGT18000.09.9951035
CTAATAC3050.09.9876613
AGTACCG1054.0174906E-59.9724385
ATAGGAC3250.09.9588513
TAGGACT4400.09.9518344
TTAGGAC12100.09.9128573
TAGGACA7600.09.76964954
TGTAGGA20300.09.7089982
GGACGTG18250.09.5965186
TCCTACA21350.09.5882792
ATGGCGA19350.09.56508317
TATGGCG18400.09.54274616
CAGGACG1502.5738154E-79.5191464
Produced by FastQC (version 0.11.5)