##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513151_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2773297 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.700973967086828 31.0 31.0 34.0 27.0 34.0 2 30.815187482624474 33.0 31.0 34.0 27.0 34.0 3 30.914036974763253 33.0 31.0 34.0 27.0 34.0 4 34.29697576566809 37.0 35.0 37.0 31.0 37.0 5 34.02898283162604 37.0 35.0 37.0 30.0 37.0 6 33.945495559977886 37.0 35.0 37.0 30.0 37.0 7 33.80606909393404 37.0 35.0 37.0 29.0 37.0 8 33.777146840024706 37.0 35.0 37.0 29.0 37.0 9 35.33494068612197 39.0 35.0 39.0 29.0 39.0 10 35.175278017464414 39.0 35.0 39.0 28.0 39.0 11 35.18961366200591 39.0 35.0 39.0 28.0 39.0 12 35.12416232376121 39.0 35.0 39.0 28.0 39.0 13 35.089530980634244 39.0 35.0 39.0 28.0 39.0 14 36.24264260192832 40.0 36.0 41.0 27.0 41.0 15 36.22628193085703 40.0 36.0 41.0 27.0 41.0 16 36.18711266770202 40.0 36.0 41.0 27.0 41.0 17 36.11924218718731 40.0 36.0 41.0 27.0 41.0 18 36.071121845226095 40.0 36.0 41.0 27.0 41.0 19 36.1513350355191 40.0 37.0 41.0 27.0 41.0 20 36.114745012885386 40.0 37.0 41.0 27.0 41.0 21 36.06196559546273 40.0 37.0 41.0 27.0 41.0 22 36.00419897327982 40.0 37.0 41.0 27.0 41.0 23 35.92838632140734 40.0 37.0 41.0 27.0 41.0 24 35.86611891910603 40.0 36.0 41.0 27.0 41.0 25 35.80817669366101 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5318042094890636 1101 2 0.5726953747576502 1101 3 0.4379807999119265 1101 4 0.6047445963023037 1101 5 0.46593840348659654 1101 6 0.48348207999694637 1101 7 0.5437768473703883 1101 8 0.5895391376325989 1101 9 0.6960763913189965 1101 10 0.8511381954755635 1101 11 0.7824243307439005 1101 12 0.7238738248653362 1101 13 0.765373816026873 1101 14 0.7630489828986384 1101 15 0.8969182581915405 1101 16 0.8506305311846276 1101 17 0.9410292918032184 1101 18 0.9041187739692234 1101 19 0.8704573405035845 1101 20 0.7924728612832936 1101 21 0.687069363086124 1101 22 0.8688149236168172 1101 23 0.9469659398565256 1101 24 0.9431833791687723 1101 25 0.8554719902057499 1102 1 0.5077772561760518 1102 2 0.6172389332367132 1102 3 0.46271642366568244 1102 4 0.5864365126003506 1102 5 0.5453250780986849 1102 6 0.5244242235798708 1102 7 0.5079860869783133 1102 8 0.5731727926026906 1102 9 0.5722017761753264 1102 10 0.6687663344898738 1102 11 0.6668212045263004 1102 12 0.6940347960402633 1102 13 0.6912152284782707 1102 14 0.8275838319738398 1102 15 0.8099574581487587 1102 16 0.7563290788533337 1102 17 0.8886329069698107 1102 18 0.9093117178241314 1102 19 0.8328440227555021 1102 20 0.918450873858454 1102 21 0.9112567527861088 1102 22 0.8558611369360918 1102 23 0.834639383050586 1102 24 0.9201657999246535 1102 25 0.9615109686079606 1103 1 0.37059216543788054 1103 2 0.35793865355748977 1103 3 0.35809532328191196 1103 4 0.4779825642248454 1103 5 0.455680953253335 1103 6 0.36096729472068745 1103 7 0.3977438483919471 1103 8 0.4561775523721039 1103 9 0.5226187737567258 1103 10 0.5162313025509775 1103 11 0.5174069461084656 1103 12 0.443105263318877 1103 13 0.5221137497621626 1103 14 0.5404488962028111 1103 15 0.5186777591145812 1103 16 0.41181596022288147 1103 17 0.5385258436736962 1103 18 0.5288624301687364 1103 19 0.4367179311871183 1103 20 0.49443934091735287 1103 21 0.4069356817569485 1103 22 0.34878703544837464 1103 23 0.3454570080643151 1103 24 0.5458105658891483 1103 25 0.4852458838487621 1104 1 0.2717097192892268 1104 2 0.3783013676496054 1104 3 0.37531908273192016 1104 4 0.5341268529398775 1104 5 0.42728273027432095 1104 6 0.4402137162765598 1104 7 0.42407630955455033 1104 8 0.4399657116331994 1104 9 0.37513556322245023 1104 10 0.48891451394523955 1104 11 0.5084734668639044 1104 12 0.4766146791078185 1104 13 0.5469344244367491 1104 14 0.5711705189420329 1104 15 0.5429337086161112 1104 16 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-0.37439624280388273 2216 5 -0.6547834655722298 2216 6 -0.7370794770326938 2216 7 -0.9355945203834963 2216 8 -0.9474717806243476 2216 9 -1.1597964849361517 2216 10 -1.0237940444823153 2216 11 -0.9528311720500611 2216 12 -1.1276505970224449 2216 13 -1.052699097171157 2216 14 -1.2709953209226867 2216 15 -1.2904427002125232 2216 16 -1.2603541978317452 2216 17 -1.393109982884745 2216 18 -1.3470427028918337 2216 19 -1.3333788391494252 2216 20 -1.4093965996395497 2216 21 -1.5542954127497097 2216 22 -1.4377461584328302 2216 23 -1.4858058699554562 2216 24 -1.3876683805818288 2216 25 -1.5502609931335058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70423.0 3 4.0 4 1201.0 5 9734.0 6 10749.0 7 8189.0 8 6486.0 9 5768.0 10 5228.0 11 4802.0 12 4465.0 13 4176.0 14 4019.0 15 4131.0 16 4156.0 17 4386.0 18 4881.0 19 5854.0 20 7144.0 21 9460.0 22 12076.0 23 14297.0 24 16907.0 25 18610.0 26 20759.0 27 22842.0 28 25686.0 29 31462.0 30 39652.0 31 50581.0 32 64330.0 33 84554.0 34 111669.0 35 150847.0 36 216585.0 37 353837.0 38 1363347.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.61520955401588 19.18515601858207 13.520984300607603 27.678650126794444 2 13.729788558335237 20.246529282835052 39.987731955119585 26.03595020371013 3 16.76302579068512 24.562110796705387 29.98273072369771 28.69213268891178 4 11.199892111792664 17.283243684915952 37.59464107158375 33.92222313170763 5 12.681881021727387 38.109155497560366 36.20443208518172 13.004531395530531 6 32.1250115305936 37.52053350021795 17.740437746551223 12.614017222637223 7 27.888831619644368 31.463358243651257 22.4299624992651 18.217847637439274 8 25.12941296964268 36.85703734637957 20.353773261697295 17.65977642228045 9 26.24303186366162 14.70103228844794 19.65228102680012 39.40365482109031 10 14.217772955414897 29.024217949647173 35.053481711140705 21.70452738379722 11 34.44055937752069 22.17595158398109 24.48165486783421 18.901834170664014 12 22.864085599198354 24.785156440150477 31.772904236365594 20.57785372428557 13 28.833586882328145 21.494019573092967 25.74859454288524 23.923799001693652 14 20.840953738133084 21.562235870038684 27.658535670706204 29.93827472112203 15 23.22124172059466 30.318209697699167 23.171265104314468 23.289283477391713 16 22.489008569944005 27.899536183827404 27.09616027421513 22.515294972013457 17 21.926275624287715 28.390917353106655 27.430863111123934 22.2519439114817 18 21.919918975419947 27.494434609730163 28.814402349943663 21.771244064906227 19 23.481545611595152 26.96004070245632 27.32210073425241 22.23631295169612 20 23.678134725563112 27.052025080617042 27.53188713650215 21.7379530573177 21 23.184629591634735 26.636502592420445 26.877300752863853 23.30156706308097 22 23.16130204731269 27.654803277258015 26.715270721387636 22.468623954041654 23 22.434524605853074 27.408343102640654 27.140560108555604 23.016572182950668 24 22.985637672416622 26.975004840808616 27.638330838709308 22.40102664806546 25 22.83937440458891 26.985382725379996 27.30788729936314 22.86735557066795 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 106.0 1 106.0 2 208.5 3 311.0 4 311.0 5 311.0 6 820.5 7 1330.0 8 1330.0 9 1330.0 10 2260.5 11 3191.0 12 3191.0 13 3191.0 14 6396.0 15 9601.0 16 9601.0 17 9601.0 18 18752.5 19 27904.0 20 27904.0 21 27904.0 22 45807.0 23 63710.0 24 63710.0 25 63710.0 26 92173.0 27 120636.0 28 120636.0 29 120636.0 30 156271.0 31 191906.0 32 191906.0 33 191906.0 34 224319.5 35 256733.0 36 256733.0 37 256733.0 38 283216.0 39 309699.0 40 309699.0 41 309699.0 42 328303.5 43 346908.0 44 346908.0 45 346908.0 46 362005.0 47 377102.0 48 377102.0 49 377102.0 50 365271.0 51 353440.0 52 353440.0 53 353440.0 54 315018.5 55 276597.0 56 276597.0 57 276597.0 58 236598.0 59 196599.0 60 196599.0 61 196599.0 62 160137.5 63 123676.0 64 123676.0 65 123676.0 66 94875.0 67 66074.0 68 66074.0 69 66074.0 70 48032.5 71 29991.0 72 29991.0 73 29991.0 74 20799.0 75 11607.0 76 11607.0 77 11607.0 78 7897.5 79 4188.0 80 4188.0 81 4188.0 82 2788.0 83 1388.0 84 1388.0 85 1388.0 86 918.0 87 448.0 88 448.0 89 448.0 90 287.5 91 127.0 92 127.0 93 127.0 94 73.0 95 19.0 96 19.0 97 19.0 98 12.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18162497561566612 2 0.008617901364332778 3 0.005913539011508684 4 0.0020192572234419897 5 0.07882314804364625 6 0.32135036384491095 7 0.029495578728134783 8 0.014098742399389606 9 0.004976026729196332 10 0.0038221654586580523 11 0.0 12 0.0 13 0.0 14 0.001478384752877171 15 0.0 16 0.0 17 0.019363234446220512 18 0.029026822586978602 19 0.0 20 0.0 21 7.21163294086425E-4 22 1.0817449411296373E-4 23 0.005625073693874114 24 0.0 25 1.0817449411296373E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2773297.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.08495817250837 #Duplication Level Percentage of deduplicated Percentage of total 1 78.91340835170205 53.72816106875706 2 13.648457324285843 18.585092920865343 3 3.9655909437070482 8.09991280594717 4 1.5291147955174096 4.164388675750661 5 0.711626956195169 2.4225545773488766 6 0.36265389845232193 1.4814765304334063 7 0.214473464238148 1.0221691789237095 8 0.14903242960916957 0.8117493389030087 9 0.08862220207945166 0.5430455029561546 >10 0.3836656190988569 4.410599092686955 >50 0.01869200127655316 0.8818392387758783 >100 0.011856275794742412 1.574581813573824 >500 0.0017999074241091153 0.8655395802986666 >1k 0.001005830619189283 1.4088896747793191 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3558 0.12829495001797497 No Hit GTCCTACAGTGGACATTTCTAAATT 3325 0.11989339764186815 No Hit CCCATGTACTCTGCGTTGATACCAC 3303 0.11910011801837307 No Hit CTTTAGGACGTGAAATATGGCGAGG 3044 0.10976105335995388 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2986 0.10766967980710325 No Hit GTATCAACGCAGAGTACTTTTTTTT 2948 0.10629946954833903 No Hit CTGTAGGACGTGGAATATGGCAAGA 2923 0.10539801543073102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.6058164704321246E-5 0.0 0.0 0.0 3 0.0 3.6058164704321246E-5 0.0 0.0 0.0 4 0.0 3.6058164704321246E-5 0.0 3.6058164704321246E-5 0.0 5 0.0 3.6058164704321246E-5 0.0 3.6058164704321246E-5 0.0 6 0.0 3.6058164704321246E-5 0.0 3.6058164704321246E-5 3.6058164704321246E-5 7 0.0 3.6058164704321246E-5 0.0 7.211632940864249E-5 3.6058164704321246E-5 8 0.0 3.6058164704321246E-5 0.0 7.211632940864249E-5 3.6058164704321246E-5 9 0.0 3.6058164704321246E-5 0.0 1.0817449411296375E-4 3.6058164704321246E-5 10 0.0 3.6058164704321246E-5 0.0 1.0817449411296375E-4 3.6058164704321246E-5 11 0.0 3.6058164704321246E-5 0.0 1.0817449411296375E-4 3.6058164704321246E-5 12 0.0 7.211632940864249E-5 0.0 1.0817449411296375E-4 7.211632940864249E-5 13 0.0 7.211632940864249E-5 0.0 1.0817449411296375E-4 1.0817449411296375E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 655 0.0 13.079742 4 GGCGAGG 705 0.0 11.3065815 19 TGGCGAG 1705 0.0 10.855388 18 GTCCTAA 1120 0.0 10.715629 1 CTAGGAC 375 0.0 10.407976 3 GTAGGAC 1825 0.0 10.223672 3 GTCCTAC 1900 0.0 10.126582 1 AGGACGT 1800 0.0 9.995103 5 CTAATAC 305 0.0 9.987661 3 AGTACCG 105 4.0174906E-5 9.972438 5 ATAGGAC 325 0.0 9.958851 3 TAGGACT 440 0.0 9.951834 4 TTAGGAC 1210 0.0 9.912857 3 TAGGACA 760 0.0 9.7696495 4 TGTAGGA 2030 0.0 9.708998 2 GGACGTG 1825 0.0 9.596518 6 TCCTACA 2135 0.0 9.588279 2 ATGGCGA 1935 0.0 9.565083 17 TATGGCG 1840 0.0 9.542746 16 CAGGACG 150 2.5738154E-7 9.519146 4 >>END_MODULE