FastQCFastQC Report
Thu 26 May 2016
SRR1513150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675417
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29680.1771499274508973No Hit
CCCATGTACTCTGCGTTGATACCAC26420.15769208501525292No Hit
GTCCTACAGTGGACATTTCTAAATT21090.12587910949930675No Hit
GTCCTAAAGTGTGTATTTCTCATTT20230.12074605904082386No Hit
CTGTAGGACGTGGAATATGGCAAGA17160.10242226263670476No Hit
CTTTAGGACGTGAAATATGGCGAGG17140.10230288937022843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5100.013.446264
CCAACGA951.0512958E-611.98919419
GTATAAG2000.011.4560951
AGGACCT9300.011.350535
GTCCTAC11850.011.2788561
GGACCTG8850.011.25733956
CTTAGAC1803.6379788E-1211.111843
GTCCTAT2150.011.1008671
TAGGACT3450.011.0428224
TGTAGGA14050.010.9891542
CGTGGAA6150.010.9562959
GACGTGG6150.010.9549877
GTAGGAC12550.010.9284343
TAATACC1605.2023097E-1010.7149894
TGGCGAG12950.010.48090218
TTATACT2100.010.4315234
ACGTGGA6600.010.3515228
GTCCTAA8700.010.3148751
GGACGTG11400.010.2374076
GTATAAA2800.010.2286561
Produced by FastQC (version 0.11.5)